Integrative taxonomy and phylogeny
of barnacles (Crustacea: Cirripedia)
from the Moluccas, eastern Indonesia
Dissertation
Submitted to the Department of Earth Sciences of the
Freie Universität Berlin
In fulfillment of the requirements for the Degree of
Doctor rerum naturalium (Dr. rer. nat.)
by
Pipit Pitriana
from Bandung, Indonesia
Berlin, September 2020
II
III
Reviewers of dissertation
1st Reviewer
: Prof. Dr. Hannelore Hoch
Humboldt-Universität zu Berlin
2nd Reviewer
: Prof. Dr. Frank Riedel
Freie Universität Berlin
Day of Disputation : 8 December 2020
IV
V
Abstract
Barnacles (Cirripedia) are crustaceans, with sessile adult forms that are permanently
attached to hard substrata or to other living organisms. They are adjusted to various lifestyles,
from parasites of decapod crustaceans to free-living groups. As cosmopolite animals, barnacles
are abundant, with over 1000 species found worldwide in polar, tropical, and temperate
waters, from the ocean shore to its depths. However, knowledge of the barnacle fauna of the
Maluku Islands, eastern Indonesia (also known as the Moluccas), remains sparse. The lack of
studies on barnacles in the Moluccas is unfortunate, given that the exceptionally high marine
biodiversity of this region—part of the global epicentre of marine biodiversity—suggests that
many barnacle species may not yet have been discovered.
In the doctoral thesis presented here, barnacles from the Moluccas Islands were studied
to establish a taxonomic checklist, including information on their morphology, distribution,
and substrate. This doctoral thesis is divided into three major sections. In its first section,
it focuses on integrative biodiversity discovery of barnacles from the Moluccas, including
morphological and molecular data of recently collected material. In the second section, deepsea barnacles collected from the Moluccas by previous scientific expedition and deposited
in the Muséum National d’Histoire Naturelle, Paris were investigated. In the third section of
this thesis, a modern non-destructive imaging method, X-ray micro-computed tomography
(Micro-CT scanning), was used to study sponge-dwelling barnacles from the Moluccas that still
embedded in their sponge host.
The first section provides the most comprehensive overview of barnacle species from
the Moluccas Islands to date. Morphological analysis of recently collected material revealed
24 intertidal species and one deep-water species from three families of stalked barnacles
(Heteralepadidae: one genus and species; Lepadidae: two genera and species; Pollicipedidae:
one genus and species) and four families of acorn barnacles (Pachylasmatidae: one genus and
species; Chthamalidae: five genera and species; Tetraclitidae: five genera and eight species;
Balanidae: two genera and seven species). Including previous records from the literature, a
total of 97 species from the Moluccas Islands are recorded from the superorder Thoracica
(free living or epizoic). Of these, 21 species are new records, and two (Amphibalanus sp. and
Microeuraphia sp.) are currently unidentified.
The second section examines the deep-sea barnacles collected by the Karubar expedition
(1991) and deposited in the Muséum National d’Histoire Naturelle, Paris. It identifies 40
species from three families of stalked barnacles (Heteralepadidae: two genera, three species;
Poecilasmatidae: four genera, five species; Scalpellidae: 12 genera, 21 species) and five families
of acorn barnacles (Verrucidae: two genera and species; Pachylasmatidae: one genus and
species; Archaeobalanidae: three genera, six species; Pyrgomatidae: one genus and species;
Balanidae: one genus and species). Thirty of the species are new records for the Indonesian
VI
Kei Islands and Tanimbar Island, which increases the total number of species recorded from
Kei, Aru, and Tanimbar to 40.
The third section explores the sponge-dwelling barnacles of the Moluccas using a modern
non-destructive imaging method, X-ray micro-computed tomography (Micro-CT scanning). The
results reveal the advantage of using micro-CT scanning when examining sponge barnacles, as
their deep anchors in sponge tissue makes it almost impossible to pull out the barnacle without
breaking it. Although there are some methodological limitations regarding contrast-enhancing
techniques, this study demonstrates that micro-CT is a useful non-destructive technique for
the integrative taxonomy of the sponge-inhabiting barnacles.
Overall, this doctoral thesis demonstrates that 126 species now are recorded from the
Moluccas Islands, including 46 new records; five further species are proposed. This shows that
the Indo-Malayan region (including the Moluccas) has not been replaced by other areas as
the centre of benthic biodiversity. However, a comparison of the number of species previously
recorded from the Moluccas with those recorded in this study indicates that species diversity
for each island has been heavily underestimated. Molecular results also indicate that the
barnacle fauna of the region is understudied. Nonetheless, a modern non-destructive imaging
method, X-ray micro-computed tomography (Micro-CT scanning) is proven to be a very useful
technique for studying the morphology and taxonomy of barnacles from the Moluccas. When
paired with classic morphological examination, this technique has the potential to be an
extremely useful tool for understanding the integrative taxonomy of barnacles and helping
identify further species of barnacles.
VII
Kurzfassung
Rankenfußkrebse (Cirripedia) sind Krustentiere mit festsitzenden adulten Formen,
welche dauerhaft an harte Substrate oder an andere lebende Organismen gebunden sind.
Sie sind an verschiedene Lebensstile angepasst, vom Parasiten der Zehnfußkrebse bis hin zu
den frei lebenden Gruppen. Als kosmopolitische Tiere sind Seepocken in über 1000 Arten
weltweit reichlich vorhanden. Sie sind lokalisiert von der Meeresküste bis in die Tiefe des
Ozeans, in polaren, tropischen sowie gemäßigten Gewässern. Dennoch sind die Kenntnisse
über die Seepockenfauna der Maluku-Inseln im Osten Indonesiens (ebenfalls bekannt als die
Molukken) spärlich und lückenhaft. Das Fehlen von Studien über jene Seepockenart auf den
Molukken stellt ein Problem von wissenschaftlicher Relevanz dar, denn die bemerkenswert
hohe marine Biodiversität dieser Region, die einen Teil des globalen Epizentrums dieser
Biodiversität darstellt, deutet darauf hin, dass eine Vielfalt an Seepockenarten möglicherweise
unentdeckt geblieben sind.
Im Rahmen der vorliegenden Doktorarbeit wurden Seepocken von den Molukkeninseln
untersucht, um eine taxonomische Prüfliste mit Information über deren Morphologie,
Verbreitung und dem jeweiligen Substrat zu erstellen. Die Doktorarbeit gliedert sich in drei
Hauptabschnitten. Der erste Teil fokussiert sich auf das Material der kürzlich gesammelten
molekularen und biogeographischen Daten über die Seepocken und die darauf aufbauende
Erstellung der taxonomischen Liste. Der zweite Teil der Dissertation behandelt die Analyse von
Tiefsee-Rankenfußkrebsen, die in früheren wissenschaftlichen Expeditionen auf den Molukken
gesammelt und in Museumssammlungen deponiert wurden. Im dritten Abschnitt wurde eine
moderne zerstörungsfrei Bildgebungsmethode, die Mikro-Röntgen-Computertomographie
(Mikro-CT-Scanning), zur Erforschung von schwammbewohnenden Seepocken der Molukken
verwendet.
Den bisher umfangreichsten Überblick über die auf den Molukken lebenden
Seepockenarten, bietet der erste Abschnitt. Eine morphologische Analyse des kürzlich
angeeigneten Materials ergab sowohl vierundzwanzig intertidale Arten, als auch eine
Tiefseeart aus drei Familien von gestielten Seepocken (Heteralepadidae: eine Gattung und Art;
Lepadidae: zwei Gattungen und Arten; Pollicipedidae: eine Gattung und Art) und vier Familien
von Eichel-Seepocken (Pachylasmatidae: eine Gattung und Art; Chthamalidae: fünf Gattungen
und Arten; Tetraclitidae: fünf Gattungen und acht Arten; Balanidae: zwei Gattungen und sieben
Arten). Unter Berücksichtigung früherer literarischer Aufzeichnungen sind insgesamt 97 Arten
von den Molukkeninseln aus der Überordnung Thoracica (freilebend oder epizoisch) erfasst.
Darunter fallen einundzwanzig neu erfasste Arten neben zwei derzeit unidentifizierten Arten
(Amphibalanus sp. und Microeuraphia sp.).
VIII
Die von der Karubar-Expedition (1991) zusammengetragenen und im Muséum
National d’Histoire Naturelle hinterlegten Tiefsee-Rankenfußkrebse werden im zweiten
Abschnitt aufgearbeitet. Es werden vierzig Arten aus drei Familien von gestielten Seepocken
(Heteralepadidae: zwei Gattungen und drei Arten; Poecilasmatidae: vier Gattungen und
fünf Arten; Scalpellidae: zwölf Gattungen und einundzwanzig Arten) und fünf Familien von
Eichelrassen (Verrucidae) identifiziert: Verrucidae: zwei Gattungen und Arten; Pachylasmatidae:
eine Gattung und Art; Archaeobalanidae: drei Gattungen und sechs Arten; Pyrgomatidae: eine
Gattung und Art; Balanidae: eine Gattung und Art. Durch die dreißig neu erfassten Arten erhöht
sich die Gesamtzahl der Arten für die indonesischen Kei- und Tanimbar-Inseln auf vierzig.
Der dritte Abschnitt dieser Doktorarbeit befasst sich mit den schwammbewohnenden
Seepocken der Molukken, die mit einer modernen zerstörungsfreien Bildgebungsmethode,
der Röntgen-Mikro-Computertomographie (Mikro-CT-Scanning), untersucht werden. Die
Ergebnisse deuten auf den Vorteil des Mikro-CT-Scannings bei der Untersuchung von
Schwamm-Seepocken, da diese es ermöglicht die Seepocke aus ihrer tiefen Verankerung im
Schwammgewebe zu befreien, ohne sie dabei zu zerbrechen. Obwohl bei kontrastverstärkenden
Techniken diverse methodische Einschränkungen bekannt sind, erweist sich die Studie als
nützlich für das Verständnis über Mikro-CT als zerstörungsfreie Technik für die integrative
Taxonomie der schwammbewohnenden Seepocken.
Zielsetzung dieser Doktorarbeit ist das Darstellen der nun bekannten 126 Arten auf den
Molukken-Inseln, worunter sich 46 neue Datensätze und 5 weitere bislang vorgeschlagene
Arten befinden. Dadurch lässt sich nachweisen, dass die Region Indo-Malayan (inklusive der
Molukken) fortwährend als Zentrum der benthischen Biodiversität bestehen geblieben ist und
nicht von einem anderen Gebiet überholt wurde. Vergleicht man jedoch die in dieser Studie
zusammengetragenen Daten mit der Anzahl der früher auf den Molukken nachgewiesenen
Arten, offenbart sich eine starke Unterschätzung der Artenvielfalt der individuellen Inseln. Die
molekularen Ergebnisse deuten ebenfalls auf eine schwache Erforschung der Seepockenfauna
in jener Region. Nichtsdestotrotz konnte die Mikro-Röntgen-Computertomographie als
eine effektive Technik zur Analyse der Morphologie und Taxonomie von Seepocken von
den Molukken sichergestellt werden. In Assoziation mit der klassischen morphologischen
Untersuchung besitzt diese Technik das Potenzial, um sich als vorteilhaftes Instrument zum
Verständnis der integrativen Taxonomie von Seepocken herauszukristallisieren und zur
zukünftigen Identifizierung weiterer Seepockenarten beizutragen.
IX
Acknowlegments
This dissertation would not have been possible without the support and help of numerous
people to whom I would like to express my gratitude.
I would like to express my sincere gratitude toward Prof. Frank Riedel (FU Berlin) and Dr.
Thomas von Rintelen (MfN Berlin) for giving me the great opportunity to become a doctoral
student in Freie Universitat Berlin and to do the doctoral research at Museum für Naturkunde
Berlin. I thank both for their invaluable supports to me. I am very grateful to Dr. Thomas von
Rintelen who always supported the barnacles project albeit his focus is on mollusks and kindly
helped in financed molecular labwork, international workshop of Dest Taxonomy, visiting
research to MNHN Paris, and commented on the manuscript of the checklist of the Moluccan
barnacles.
I also would like to express my deeply sincere gratitude to my supervisor at the Museum
für Naturkunde Berlin, Dr. Kristina von Rintelen, for her constant encouragement and support
over the years. I greatly benefited from the inspiring discussions as well as from her constructive
criticism, her broad knowledge, her great support, her beliefs, and her patience. Thank you
very much for always open-heartedly and provide a time for our weekly meeting to discuss
everything regarding my amazing journey as a doctoral student, as a wife, and as a mother in
Germany. I would like to acknowledge my first reviewer Prof. Dr. Hannelore Hoch (HU Berlin)
for her kindness, support, and encouragement.
I would like to thankful to the Ministry of Research, Technology and Higher Education,
the Republic of Indonesia within the Program for Research and Innovation in Science and
Technology (RISET-Pro) who funded my study. The study took place at the Museum für
Naturkunde Berlin. I thank all the institutions and organizations that made this doctoral study
possible and successful.
I would like to thank Dr. Luis Valente, Dr. Andreas Wessel, Robert Schreiber, Bernhard
Schurian, Christine Zorn, Philipp Vogt, Anke Sänger, Petra Ebber, Michael Czura, Kristin Mahlow,
Dr. Charles Oliver Coleman, Dr. Christian Mitgutsch, Dr. Carsten Lüter, Dr. Peter Giere, Dr.
Frederik Berger, Lukas Kirschey, Sarah Ehlers, Hans-Ulrich Raake, Martina Rissberger, Isabelle
Waurick, Nora Lentge-Mass, Tina Aschenbach, Falko Glöckler, Dr. Jörg Plötner for supporting
the molecular, morphological, and logistic work progress at the Museum für Naturkunde Berlin.
I would like to thank Dr. Romanus E. Prabowo, Prof. Zainal Arifin, Dr. Daisy Wowor, Dr.
Abdul Basit, Bani Wahyudi, Ristiyanti Marwoto, Prof. Dwi Listyo Rahayu, Dr. Augy Syahailatua,
Dr. Nugroho Dwi Hananto, Daniel Josep Tala, Ading, Nurul Fitriya, Doni Nurdiansah, M. Masrur
Islami, Frilla R. Saputra, Terry Indra Budi, Dharma Arif Nugroho, Rianta Pratiwi, Dewi Citra
Murniati, and Dr. Evy Arida (all Indonesian colleagues) for supporting administration things,
field works, and for their moral and general support.
X
I would like to thank Dr. Diana S. Jones (Australia), Prof. William Anderson Newman (USA),
Prof. Benny Kwok Kan Chan (Taiwan), Dr. Laure Corbari (France), and Dr. Martin Husemann
(Hamburg) for their supports, comments, suggestions, and sharing knowledge when writing
and publish manuscripts. I also thanks Prof. Keiji Baba (Japan), Dr. Enrique Macpherson (Spain),
Ferran Palero, PhD (Spain), Paula Martin-Lefèvre (France) and Sébastien Soubzmaigne (France)
for the great time working in the Crustacean Laboratory of the Muséum National d’Histoire
Naturelle (MNHN).
I am grateful to my lunch group: Isa, Tine, Nora, and Virginia for our daily small talk after
lunchtime; Marcy and all my fellow doctoral students in MfN Berlin for the nice time in all
activities related to the MfN‘s doctoral program.
Finally and most importantly, I would like to thank with all my heart to my family. My
beloved husband, Abdul Basit, for your great moral, support, patience, and love. Our beloved
sons, Mizan Ilman Ramadhan and Nahyan Ilzam Ahlami, for their love, patience, and support,
whose always trying to understand why their mom lives in another city to work and finish her
study. You all are the greatest happiness and source of strength for me! Also, greatest thanks
to my parents, Uji Sanudji and Dede Painem, in-laws, Abdussamad Abbas and Hamidah for
your love, prayers, and support throughout this study.
This work is dedicated to my beloved and only one big sister, Santi Sanita, who passed
away when delivered her fourth baby at the beginning of my study here. Thank you very much
for all you did for me. Rest in peace in heaven, my dearest sister. Until we meet again.
XI
Table of contents
Abstract ..........................................................................................................................................
Kurzfassung ..................................................................................................................................
Acknowlegments .......................................................................................................................
Table of contents ........................................................................................................................
List of figures .................................................................................................................................
List of tables...................................................................................................................................
V
VII
IX
XI
XIII
XVI
Chapter I
General introduction ................................................................................................................
1
1.1
1.2
1.3
1.4
1.5
1.6
Preface
..........................................................................................................
Scientific background ..........................................................................................
1.2.1 The Moluccas, eastern Indonesia ...............................................................
1.2.2 Barnacles (Crustacea, Cirripedia) ................................................................
1.2.2.1 General description and taxonomy.................................................
1.2.2.2 Distribution ....................................................................................
Aims and objectives of the thesis ........................................................................
Material and methods .........................................................................................
1.4.1 Fieldwork ....................................................................................................
1.4.2 Morphological analysis ...............................................................................
1.4.3 Molecular phylogenetic analysis.................................................................
1.4.4 X-ray micro-computed tomography and 3D reconstruction ......................
Overview of the integrated manuscripts .............................................................
Contribution to the individual manuscripts .........................................................
Chapter 2
An annotated checklist and integrative biodiversity discovery of
barnacles (Crustacea, Cirripedia) from the Moluccas, East Indonesia .........
2.1
2.2
2.3
2.4
2.5
2.6
2.7
Abstract
..........................................................................................................
Introduction .........................................................................................................
Material and methods .........................................................................................
2.3.1 Sampling......................................................................................................
2.3.2 Morphological analyses ..............................................................................
2.3.3 Molecular phylogenetic analyses ................................................................
Results
..........................................................................................................
2.4.1 Checklist tabulation ...................................................................................
2.4.2 Molecular results ........................................................................................
Molecular study of Moluccan barnacles ..............................................................
Discussion ..........................................................................................................
Acknowledgements..............................................................................................
1
1
1
4
4
6
7
7
7
8
9
9
9
10
11
11
12
13
13
13
15
16
16
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70
73
74
XII
Chapter 3
New insights gained from museum collections: Deep-sea barnacles
(Crustacea, Cirripedia, Thoracica) in the Muséum National d’Histoire
Naturelle, Paris, collected during the Karubar expedition in 1991 ......................
75
3.1
3.2
3.3
3.4
3.5
3.6
Abstract
..........................................................................................................
Introduction .........................................................................................................
Material and Methods .........................................................................................
Results
..........................................................................................................
Discussion ..........................................................................................................
Acknowledgements..............................................................................................
75
76
76
77
146
147
Chapter 4
Exploring sponge-inhabiting barnacles of eastern Indonesia using
micro-CT scanning ..................................................................................................................
149
4.1
4.2
4.3
4.4
4.5
4.6
4.7
Abstract
..........................................................................................................
Introduction .........................................................................................................
Material and Methods .........................................................................................
4.3.1 Material ......................................................................................................
4.3.2 Specimen preparation, micro-CT, and 3D reconstruction .........................
Results
..........................................................................................................
Discussion ..........................................................................................................
Acknowledgements..............................................................................................
Non-invasive 3D visualization of the sponge-inhabiting barnacle Acasta sulcata
(Crustacea, Cirripedia, Balanomorpha) from the Moluccas, Indonesia. [Dataset].
149
149
150
150
151
152
166
168
169
Chapter 5
General conclusion and outlook.........................................................................................
171
5.1
5.2
General conclusion...............................................................................................
Outlook
..........................................................................................................
171
172
Chapter 6
Overall references .....................................................................................................................
175
Chapter 7
Appendix.........................................................................................................................................
193
7.1
7.2
7.3
7.4
7.5
7.6
Supplementary material for An annotated checklist and integrative biodiversity
discovery of barnacles (Crustacea, Cirripedia) from the Moluccas,
East Indonesia ......................................................................................................
Supplementary material for New insights gained from museum collections:
Deep-sea barnacles (Crustacea, Cirripedia, Thoracica) in the Muséum National
d’Histoire Naturelle, Paris, collected during the Karubar expedition in 1991 ......
List of publications, presentations, seminars, trainings, courses, and interviews
Curriculum vitae ..................................................................................................
Declaration of originality......................................................................................
Statement from proof reader .............................................................................
193
229
241
243
245
244
XIII
List of figures
Figure 1.1.
Figure 2.1.
Figure 2.2.
Figure 2.3.
Figure 2.4.
Figure 2.5.
Figure 2.6.
Figure 2.7.
Figure 2.8.
Figure 2.9.
Figure 2.10.
Figure 2.11.
Figure 2.12.
Figure 2.13.
Figure 2.14.
Figure 2.15.
Figure 2.16.
Figure 2.17.
Figure 12.8.
Figure 2.19.
Figure 2.20.
Figure 2.21.
Figure 2.22.
Figure 2.23.
Figure 2.24.
Figure 2.25.
Figure 2.26.
Figure 2.27.
Figure 2.28.
Figure 2.29.
Figure 3.1.
Figure 3.2.
Figure 3.3.
Figure 3.4.
Figure 3.5.
Figure 3.6.
Figure 3.7.
Map of Maluku Province and North Maluku Province...................................
Map of the Moluccas Islands (Maluku in grey) in eastern Indonesia. ...........
External morphology of barnacles .................................................................
Heteralepas japonica (Aurivillius, 1892) ........................................................
Dosima fascicularis (Ellis and Solander, 1786) ...............................................
Lepas anserifera Linnaeus, 1767....................................................................
Capitulum mitella (Linnaeus, 1758) ..............................................................
Pseudoctomeris sulcata (Nilsson-Cantell, 1932) ............................................
Hexechamaesipho pilsbryi (Hiro, 1936) .........................................................
Nesochthamalus intertextus (Darwin, 1854) .................................................
Euraphia hembeli Conrad, 1837 ....................................................................
Microeuraphia sp. ..........................................................................................
Chthamalus moro Pilsbry, 1916 ....................................................................
Tetraclitella divisa (Nilsson-Cantell, 1921) ....................................................
Tetraclitella karandei Ross, 1971 ..................................................................
Tesseropora rosea (Krauss, 1848) ..................................................................
Tetraclita kuroshioensis Chan, Tsang & Chu, 2007 .........................................
Tetraclita squamosa (Bruguiére, 1789) .........................................................
Yamaguchiella coerulescens (Spengler, 1790) ...............................................
Neonrosella vitiata (Darwin, 1854) ...............................................................
Newmanella spinosus Chan & Cheang, 2016 ................................................
Amphibalanus amphitrite (Darwin, 1854) ....................................................
Amphibalanus reticulatus (Utinomi, 1967) ...................................................
Amphibalanus variegatus (Darwin, 1854) ....................................................
Amphibalanus zhujiangensis (Ren, 1989) .....................................................
Amphibalanus sp. ..........................................................................................
Megabalanus tintinnabulum (Linnaeus, 1758) .............................................
Megabalanus zebra (Darwin, 1854) ..............................................................
Bayesian phylogeny of 18S gene sequences ..................................................
Bayesian phylogeny of COI gene sequences .................................................
Heteralepas japonica (Aurivillius, 1892) .......................................................
Paralepas minuta (Philippi, 1836) .................................................................
2
12
14
24
26
28
30
32
34
36
37
38
41
42
43
45
46
49
51
53
54
56
59
61
63
65
68
69
71
72
79
81
Paralepas morula (Hoek, 1907) ....................................................................
Dianajonesia amygdalum (Aurivillius, 1894) ................................................
Dianajonesia excavatum (Hoek, 1907) .........................................................
Dichelaspis orthogonia Darwin, 1852 ...........................................................
Glyptelasma gracile (Hoek, 1883) ..................................................................
82
84
86
88
89
XIV
Figure 3.8.
Figure 3.9.
Figure 3.10.
Figure 3.11.
Figure 3.12.
Figure 3.13.
Figure 3.14.
Figure 3.15.
Figure 3.16.
Figure 3.17.
Figure 3.18.
Figure 3.19.
Figure 3.20.
Figure 3.21.
Figure 3.22.
Figure 3.23.
Figure 3.24.
Figure 3.25.
Figure 3.26.
Figure 3.27.
Figure 3.28.
Figure 3.29.
Figure 3.30.
Figure 3.31.
Figure 3.32.
Figure 3.33.
Figure 3.34.
Figure 3.35.
Figure 3.36.
Figure 3.37.
Figure 3.38.
Figure 3.39.
Figure 3.40.
Figure 4.1.
Figure 4.2.
Figure 4.3.
Figure 4.4.
Figure 4.5.
Figure 4.6.
Megalasma striatum Hoek, 1883 .................................................................
Diotascalpellum rubrum (Hoek, 1883) ..........................................................
Regioscalpellum moluccanum (Hoek, 1883) .................................................
Scalpellum sp. ................................................................................................
Scalpellum stearnsi Pilsbry, 1890 ..................................................................
Alcockianum persona (Annandale, 1916) .....................................................
Annandaleum japonicum (Hoek, 1883) ........................................................
Annandaleum laccadivicum (Annandale, 1906) ............................................
Annandaleum lambda (Annandale, 1910) ....................................................
Litoscalpellum juddi (Calman, 1918) .............................................................
Litoscalpellum recurvirostrum (Hoek, 1883) ..................................................
Litoscalpellum walleni Newman & Ross, 1971...............................................
Amigdoscalpellum costellatum (Withers, 1935) ...........................................
Amigdoscalpellum tenue (Hoek, 1883) ..........................................................
Catherinum rossi (Rao & Newman, 1972)......................................................
Planoscalpellum distinctum (Hoek, 1883)......................................................
Teloscalpellum ecaudatum (Calman, 1918) ..................................................
Trianguloscalpellum balanoides (Hoek, 1883) ..............................................
Trianguloscalpellum hirsutum (Hoek, 1883) ..................................................
Verum australicum (Hoek, 1883) ...................................................................
Verum carinatum (Hoek, 1883)......................................................................
Verum novaezelandiae (Hoek, 1883) ............................................................
Altiverruca navicula (Hoek, 1913) .................................................................
Newmaniverruca albatrossiana (Pilsbry, 1912) ............................................
Hexelasma arafurae Hoek, 1913 ...................................................................
Conopea cymbiformis (Darwin, 1854) ...........................................................
Conopea navicula (Darwin, 1854) .................................................................
Solidobalanus auricoma (Hoek, 1913) ...........................................................
Solidobalanus pseudauricoma (Broch, 1931) ................................................
Striatobalanus amaryllis (Darwin, 1854) .......................................................
Striatobalanus tenuis (Hoek, 1883) ...............................................................
Pyrgomina sp. ................................................................................................
Amphibalanus amphitrite (Darwin, 1854) ....................................................
Acasta sp1. and its host sponge ....................................................................
Hard body of Acasta sp. 1 . ............................................................................
Soft body of Acasta sp. 1 ..............................................................................
Acasta sp. 2 and its host sponge ...................................................................
Hard body of Acasta sp. 2 ..............................................................................
Soft body of Acasta sp. 2 ...............................................................................
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109
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112
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XV
Figure 4.7.
Figure 4.8.
Figure 4.9.
Figure 4.10.
Figure 4.11.
Figure 4.12.
Figure 4.13.
Figure 4.14.
Figure 4.15.
Figure 7.1.
Figure 7.2.
Figure 7.3.
Figure 7.4.
Figure 7.5.
Figure 7.6.
Figure 7.7.
Figure 7.8.
Figure7.9.
Figure 7.10.
Figure 7.11.
Figure 7.12.
Figure 7.13.
Acasta sulcata and its host sponge ...............................................................
Hard body of Acasta sulcata .........................................................................
Soft body of Acasta sulcata ...........................................................................
Euacasta dofleini and its host sponge ...........................................................
Hard body of Euacasta dofleini ....................................................................
Soft body of Euacasta dofleini ......................................................................
Membranobalanus longirostrum and its host sponge ..................................
Hard body of Membranobalanus longirostrum ............................................
Soft body of Membranobalanus longirostrum ..............................................
The occurrence of Heteralepas japonica in the Moluccas ............................
The occurrence of Amphibalanus reticulatus, Dosima fascicularis,
Euraphia hembeli, Hexechamaesipho pilsbryi, Megabalanus zebra,
Nesochthamalus intertextus, Newmanella spinosus,
Pseudoctomeris sulcata, Tetraclitella divisa and Tetraclitella karandei
in the Moluccas .............................................................................................
The occurrence of Chthamalus moro and Lepas anserifera
in the Moluccas .............................................................................................
The occurrence of Amphibalanus amphitrite, Amphibalanus variegatus,
Capitulum mitella, Megabalanus tintinnabulum, Tesseropora rosea,
Tetraclita kuroshioensis and Tetraclita squamosa in the Moluccas ...............
The occurrence of Microeuraphia sp. in the Moluccas..................................
The occurrence of Yamaguchiella coerulescens in the Moluccas ..................
The occurrence of Neonrosella vitiata in the Moluccas ................................
The occurrence of Amphibalanus zhujiangensis in the Moluccas ................
The occurrence of Amphibalanus sp. in the Moluccas .................................
Bayesian phylogeny of concatenated COI and 18S gene sequences..............
ML phylogeny of concatenated COI and 18S gene sequences. ......................
ML phylogeny of 18S gene sequences. ..........................................................
ML phylogeny of COI gene sequences. ..........................................................
159
160
161
161
162
163
164
165
166
220
220
221
221
222
222
223
223
224
225
226
227
228
XVI
List of tables
Table 1.1.
Overview of the three manuscripts presented in this thesis .......................... 10
Table 1.2.
Overview of the author’s contribution to each manuscript. .......................... 10
Table 2.1.
Annotated checklist tabulation of barnacle species from the Moluccas,
Eastern Indonesia .......................................................................................... 17
Table 4.1.
Barnacle species and the host sponges from eastern Indonesia .................... 152
Table 7.1.
Table of sample information for DNA samples sequenced for this study. ...... 193
Table 7.2.
Measurements for Heteralepas japonica ...................................................... 195
Table 7.3.
Measurements for Dosima fascicularis ......................................................... 196
Table 7.4.
Measurements for Lepas anserifera .............................................................. 197
Table 7.5.
Measurements for Capitulum mitella ............................................................ 198
Table 7.6.
Measurements for Pseudoctomeris sulcata .................................................. 198
Table 7.7.
Measurements for Hexechamaesipho pilsbryi................................................ 199
Table 7.8.
Measurements for Nesochthamalus intertextus ........................................... 199
Table 7.9.
Measurements for Euraphia hembeli ............................................................ 200
Table 7.10. Measurements for Microeuraphia sp. ............................................................ 200
Table 7.11. Measurements for Chthamalus moro ............................................................ 201
Table 7.12. Measurements for Tetraclitella divisa ........................................................... 201
Table 7.13. Measurements for Tetraclitella karandei ....................................................... 202
Table 7.14. Measurements for Tesseropora rosea ........................................................... 202
Table 7.15. Measurements for Tetraclita kuroshioensis ................................................... 203
Table 7.16. Measurements for Tetraclita squamosa ........................................................ 203
Table 7.17. Measurements for Yamaguchiella coerulescens ............................................ 204
Table 7.18. Measurements for Neonrosella vitiata .......................................................... 204
Table 7.19. Measurements for Newmanella spinosus ..................................................... 205
Table 7.20. Measurements for Amphibalanus amphitrite ............................................... 205
Table 7.21. Measurements for Amphibalanus reticulatus ............................................... 206
Table 7.22. Measurements for Amphibalanus variegatus ............................................... 206
Table 7.23. Measurements for Amphibalanus zhujiangensis ........................................... 207
Table 7.24. Measurements for Amphibalanus sp.............................................................. 207
Table 7.25. Measurements for Megabalanus tintinnabulum ........................................... 208
Table 7.26. Measurements for Megabalanus zebra ........................................................ 208
Table 7.27. Kimura 2-parameter (K2P) distances of COI sequences between species for
Microeuraphia sp............................................................................................ 209
XVII
Table 7.28. Kimura 2-parameter (K2P) distances of COI sequences between species for
Amphibalanus sp. ........................................................................................... 212
Table 7.29. Checklist of barnacle species collected during the Karubar expedition (1991)
and deposited in MNHN, Paris. ...................................................................... 229
Table 7.30. Measurements for Heteralepas japonica ...................................................... 231
Table 7.31. Measurements for Paralepas minuta ............................................................ 231
Table 7.32. Measurements for Paralepas morula ............................................................ 232
Table 7.33. Measurements for Dianajonesia amygdalum ............................................... 232
Table 7.34. Measurements for Dianajonesia excavatum ................................................. 232
Table 7.35. Measurements for Dichelaspis orthogonia .................................................... 232
Table 7.36. Measurements for Glyptelasma gracile ........................................................ 232
Table 7.37. Measurements for Megalasma striatum ....................................................... 233
Table 7.38. Measurements for Diotascalpellum rubrum .................................................. 233
Table 7.39. Measurements for Regioscalpellum moluccanum ......................................... 233
Table 7.40. Measurements for Scalpellum sp. ................................................................. 233
Table 7.41. Measurements for Scalpellum stearnsi ......................................................... 234
Table 7.42. Measurements for Alcockianum persona ...................................................... 234
Table 7.43. Measurements for Annandaleum japonicum ................................................ 234
Table 7.44. Measurements for Annandaleum laccadivicum ............................................ 235
Table 7.45. Measurements for Annandaleum lambda ..................................................... 235
Table 7.46. Measurements for Litoscalpellum juddi ........................................................ 235
Table 7.47. Measurements for Litoscalpellum recurvirostrum ......................................... 236
Table 7.48. Measurements for Litoscalpellum walleni ..................................................... 236
Table 7.49. Measurements for Amigdoscalpellum costellatum ....................................... 236
Table 7.50. Measurements for Amigdoscalpellum tenue ................................................. 236
Table 7.51. Measurements for Catherinum rossi ............................................................. 237
Table 7.52. Measurements for Planoscalpellum distinctum ............................................ 237
Table 7.53. Measurements for Teloscalpellum ecaudatum .............................................. 237
Table 7.54. Measurements for Trianguloscalpellum balanoides ...................................... 237
Table 7.55. Measurements for Trianguloscalpellum hirsutum ......................................... 237
Table 7.56. Measurements for Verum australicum .......................................................... 238
Table 7.57. Measurements for Verum carinatum ............................................................ 238
Table 7.58. Measurements for Verum novaezelandiae .................................................... 238
Table 7.59. Measurements for Hexelasma arafurae ........................................................ 238
XVIII
Table 7.60. Measurements for Conopea cymbiformis ...................................................... 238
Table 7.61. Measurements for Conopea navicula ............................................................ 239
Table 7.62. Measurements for Solidobalanus auricoma .................................................. 239
Table 7.63. Measurements for Solidobalanus pseudauricoma ........................................ 239
Table 7.64. Measurements for Striatobalanus amaryllis ................................................. 240
Table 7.65. Measurements for Striatobalanus tenuis ...................................................... 240
Table 7.66. Measurements for Pyrgomina sp. ................................................................. 240
Table 7.67. Measurements for Amphibalanus amphitrite ............................................... 240
1
Chapter I
General introduction
1.1
Preface
Barnacles, belonging to subphylum Crustacea, are an ancient group that have endured
and flourished; approximately 1,400 extant species are known, and although they are
almost exclusively marine, a few species can tolerate the reduced salinity environments of
bays, estuaries, and similar habitats (Newman and Abbot, 1980; Van Syoc, 2019). Barnacles
often form a dense carpet-like layer, coating the surface of the sediment in the middle of the
intertidal zone (Stephenson & Stephenson, 1972). An abundance of barnacles can be found,
for example, on the Florida shelf, where 50% of carbonate sediments were built by barnacles
(Milliman, 1974). According to Newman (1987), barnacles have an advantage compared with
many other invertebrates in biogeographical studies because there is reasonable agreement
on their phylogeny; the group has a well-preserved fossil record, beginning in the Palaeozoic,
becoming stratigraphically useful in the Mesozoic, and being well represented in the Cenozoic.
The most diverse region in the world for marine species is the East Indies Triangle, a region
including Indonesia, Philippines, Malay Peninsula, and New Guinea (Briggs, 1999). However,
research on barnacles in this region remains very limited, particularly in the Moluccas, eastern
Indonesia. The lack of studies on barnacles in this region is unfortunate, considering that the
exceptionally high marine biodiversity of this region suggests that many barnacle species may
have not yet been discovered. This area lies on the collision zone of the Asian and Australian
continents, which have been separated for an extensive period (Turner et al., 2001). The
region’s high diversity is believed to be partly due to the Indonesian Throughflow (ITF), the
vast mass of seawater that flows from the Pacific Ocean to the Indian Ocean, as well as the
counter-intake from the Indian Ocean to the South China Sea through Malacca Strait. The
mixture of these two oceans, as well as the above-mentioned continental shelves, Australian
(of Gondwanaland origin) and Eurasian (of Laurasian origin), contribute to making the East
Indies Triangle a biodiversity hotspot (Hoeksema, 2013).
The absence of occurrence, distribution, and biogeographic data regarding barnacles in
the Moluccas, a biodiversity hotspot area, has highly motivated me to study the topic.
1.2
Scientific background
1.2.1 The Moluccas, eastern Indonesia
The Indonesian archipelago of the Moluccas (also known as the Maluku Islands) is also
known as the Spice Islands, as these islands were the only or best sources of spices such as
cloves, nutmeg, and mace until the 1700s (Delaney, 2010). Geographically, the Moluccas lie
2
Chapter 1
between the island of Sulawesi to the west; New Guinea to the east; the Philippine Sea and the
Pacific Ocean to the north; the Arafura Sea and the Timor Sea to the south; tectonically, they
are located on the Halmahera Plate, within the Moluccas Sea Collision Zone (Kastanya, 2019).
The Moluccas (Figure 1.1) were administratively one province until 1999, when they
were divided between Maluku Province and North Maluku Province. Maluku Province lies
between 2°30’ – 9° S latitude and 124° – 136° E longitude; it consists of 1,340 islands with an
area of 712,479.65 km2, including a land area of 54,185 km2 (7.6%), a sea area of 658,294.69
km2 (92.4%), and a coastline 10,622 km long. Its most prominent islands are Seram, Buru, and
Ambon; other large islands include Banda, Kei, Aru, Tanimbar, and Wetar (https://maluku.bps.
go.id/ProvinsiMalukudalamangka2019.html; Kastanya, 2019; Yushantarti and Rezky, 2020).
North Maluku Province lies between 3° N latitude and 3° S latitude, and from 124° – 129°
E longitude. It consists of approximately 600 islands and covers an area of 140,255.36 km2,
which includes a land area of 33,278 km2 (23.73%) and a sea area of 106,977.32 km2 (76.27%).
Its most prominent islands are Halmahera, Morotai, Taliabu, Mangole, Sulabesi, Ternate, and
Tidore (https://malut.bps.go.id/ProvinsiMalukuUtaradalamangka2019.html; Kastanya, 2019;
Yushantarti and Rezky, 2020).
Figure 1.1. Map of the Moluccas showing Maluku Province and North Maluku Province. Map modified
from Gebco Digital Atlas Team (2003). Map courtesy of Abdul Basit.
General introduction
3
The geology of the Moluccas is complicated. In the general, these islands have been formed
through the gradual impact of the Sunda Plate and the Australian Plate as well as through volcanic
processes. Seven different island groups represent different elements of the Moluccas’ complicated
tectonic system. The first is Halmahera, which is a mixture of Plio-Pleistocene volcanic arcs and
linked to an older impact complex that formed on the front edge of the Irian spur, moving westward
from the Australian plate. The second group consists of the Banggai Islands, Sula Islands, and Bacan
Islands, which represents small pieces of the Irian spur that have separated and collided with eastern
Sulawesi after moving about 1,000 km westward; to the north and south, these islands are bound
by crack lines and consist of a Palaeozoic granite base covered by Mesozoic metamorphic rocks. The
third group of islands, which is dominated by Buru and Seram Island, represent Palaeozoic and old
Mesozoic volcanic complexes on the front edge the Australian plate and resembles Halmahera. The
fourth group is centred around Obi Island, which has geological features similar to Buru Island. The
fifth spans from Wetar Island to the Banda Islands, which were formed from Quaternary volcanoes
that have developed over the subduction zone of the Australian plate. The sixth group, which
reaches from the Leti Islands to Tanimbar, Kai, Watubela and Gorong Island, consists of a mélange
formed from the mixture of tertiary younger deposits and materials of an ancient continent that
originated from the margins of the Australian plate. The last is the Aru Archipelago; these islands
are the only ones that are not directly controlled by plate tectonic events, being composed of
young sedimentary structures located on the shallow Arafura Seabed (Center for Data, Statistics
and Information of the Secretariat General of the Ministry of Marine Affairs and Fisheries, Republic
of Indonesia, 2013).
Although the Moluccas is far from the European centres where most of the formal work in
natural science was occurring from the 15th through the mid-19th centuries, this archipelago and its
surrounding seas have contributed importantly to the history of natural science (Lamoureux, 1990).
Georg Everhard Rumpf (1627 or 1628–1702) and Alfred Russel Wallace (1823–1913) are probably
the most renowned natural scientists whose ideas and publications reflect the contributions of the
natural phenomena that they observed in the Moluccas (Strack, 1993; Glasby & al Hakim, 2017).
After Rumphius’s death in 1702, there was a vacuum in marine research activities in the Moluccas
waters until the early 19th century, when many international research and scientific expeditions
were conducted in the region. These included, for example, the Astrolobe expedition (1826–1829),
research by Pieter Bleeker (1852–1874) which produced 90 scientific papers regarding fish in the
Moluccas, and the Swiss expedition to Ambon Bay by M. Bedot and C. Pictet in 1890 (Tapilatu &
Syahailatua, 2017; Strack, 1993).
The Moluccas have been a frequent destination for explorers, from the first
circumnavigation voyages to modern scientific expeditions. There is recorded that the ships of
Magellan (1521) and Francis Drake’s Golden Hind (1579) landed in Ternate, one of the islands
in the Moluccas (Lamoureux, 1990). Since then, at least fifty expeditions have visited the
Moluccas, primarily for scientific exploration. Some important marine scientific expeditions
in the Moluccas have included the famous British Challenger Expedition (1872–1876), which
4
Chapter 1
is considered the foundation of modern oceanography; the Dutch Siboga Expedition (1899–
1900), which produced the first bathymetric map; the Danish Mortensen Expedition to the
Kei Islands (1914–16), which suggested that the archipelago was an ideal place for a marine
laboratory to study deep-sea fauna; the Dutch Snellius I Expedition (1929–1930), which was
primarily a geological expedition; and the Danish Galathea Expedition to the Banda Sea
(1950–1952). The most recent expedition, the French Karubar Expedition of 1991, focused on
exploring deep-sea benthos in the Moluccas (Lamoureux, 1990; Strack, 1993; Nontji, 2009).
1.2.2 Barnacles (Crustacea, Cirripedia)
1.2.2.1 General description and taxonomy
Barnacles have been known since ancient times (Igić, 2007). In the Middle Ages,
Europeans used Aristotle’s mistaken observations to explain the arrival of barnacle geese
each fall from their high Arctic breeding grounds as the transformation of the goose-neck
barnacles commonly found on floating driftwood (Wilson, 2014). In 1758, Linnaeus described
the order Thoracica of barnacle as “Vermes testacea”, and Lamarck (1818) described barnacles
as “Crustaces conchyliferes”. Later barnacles were classified as molluscs, per Cuvier (1830),
who argued that Thoracica are modified molluscs (“Cirrhopoda-Mollusca”) due to their hard
shells. However, based on their larval development, Strausse (1810), Thompson (1830), and
Burmeister (1834) identified barnacles as crustaceans.
This past confusion can be attributed to the drastic adaptations that distinguish barnacles
from all other crustaceans. Not only are barnacles the only sessile group of crustaceans,
they also have significant morphological differences. Barnacles do not have an exoskeleton
like other crustaceans, but rather possess a protective shell that consists of mineralised
shell plates. These realities were observed by Charles Darwin, who comprehensively studied
barnacles for eight years from 1846 to 1854 and produced four incomparable monographs on
extant barnacles (Darwin 1851a; 1854a) and fossil barnacles (Darwin 1851b; 1854b). Through
these four monographs, Darwin presented an understanding of barnacles that remains the
foundation of studies today. Owing to his observations and taxonomic description of barnacles,
Darwin has been recognised as history’s greatest cirripedologist (Anderson, 1994).
Nonetheless, as knowledge has advanced, the classification of barnacles has continued
to change. Newman (1987) placed barnacles under the superclass Maxillopoda and class
Thecostraca. According to Anderson (1994), maxillopodan crustaceans are crustaceans with
six head segments, six thoracic segments, and five abdominal segments; while Thecostraca
includes all maxillopodans with a bivalve carapace and a reproductive system, usually
hermaphroditic, which includes paired female openings on the first thoracic segment. The
superclass Multicrustacea constitutes the largest superclass of crustaceans, containing
approximately four-fifths of all described crustacean species, including barnacles (Regier et
al., 2010). This classification, however, has changed based on recent work which indicates
General introduction
5
that Maxillopoda is not monophyletic (Regier et al. 2005), leading to the redefinition of
Thecostraca’s taxonomic placement within Arthropoda (Newman 1992; Regier et al. 2010;
Oakley et al. 2013). Recent works using multiple lines of evidence have placed Thecostraca
together with two other subclasses in the class Hexanauplia (Regier et al. 2010; Oakley et al.
2013).
Even though the higher classification of barnacles has changed, the majority of barnacles
still fall under the classification of Cirripedia, as defined by Burmeister in 1834. Based on
generally agreed recent advances, this study employs the following classification:
Kingdom
Phylum
Subphylum
Superclass
: Animalia
: Arthropoda von Siebold, 1848
: Crustacea Brünnich, 1772
: Multicrustacea Regier, Shultz, Zwick, Hussey, Ball, Wetzer, Martin &
Cunningham, 2010
Class
: Hexanauplia Oakley, Wolfe, Lindgren & Zaharof, 2013
Subclass
: Thecostraca Gruvel, 1905
Infraclass
: Cirripedia Burmeister, 1834
Superorder : Acrothoracica Gruvel, 1905
Superorder : Rhizocephala Müller, 1862
Superorder : Thoracica Darwin, 1854
The superorder Acrothoracica is a small group in Cirripedia with cirri bunched terminally
at the end of the thorax; and the superorder Rhizocephala is a group of parasitic barnacles
without cirri. The most abundant is the superorder Thoracica, which are characterised by six
pairs of cirri spread along the length of a well-developed thorax (Anderson, 1994). All Thoracica
are permanently attached as adults. Many form symbiotic associations with larger organisms
such as whales, turtle, lobsters, corals, and sponges, and a few become dependent on their
hosts for nutrition, support, and protection (Krüger, 1940; Newman, Zullo & Withers, 1969).
Thoracic barnacles, also called true barnacles, are considered the most ecologically
important because of their abundance and conspicuousness (Newman and Abbott, 1980).
There are two major morphological variants within Thoracica, i.e. acorn or sessile barnacles
and stalked or pedunculata barnacles. According to Anderson (1994), pedunculata barnacles
have an obvious peduncle between the basal attachment disc and plated apex, while in acorn
barnacles the peduncle has been replaced by a broad basis. In stalked barnacles, the body
is divided into two major parts: a capitulum (containing cirri, mouthparts, and most other
organs) and a peduncle/stalk by which the animal is attached to the substratum. In sessile
barnacles, meanwhile, the body lacks a peduncle; in the capitulum, certain plates become
articulated to form a rigid wall, while others (anteriorly the scuta, posteriorly the terga) form
a movable lid or operculum.
6
Chapter 1
Buckeridge and Newman (2006) revised the Pedunculata barnacles into four orders:
the Ibliformes, Cyprilepadiformes, Lepadiformes and Scalpelliformes. The bivalved
Cyprilepadiformes, restricted to the Silurian (Wills, 1963), are certainly very distinct. Anderson
(1994) recognized Ibla as distinct from Thoracica, and proposed a new superorder, the
Prothoracica, to accommodate that sole genus. However, Ibla appears to be a thoracican
cirripede, and related to Eolepas and separated from other living Thoracica by fossil forms
such as Praelepas (Newman et al. 1969, Høeg et al., 1999). The genetic work of Pérez-Losada,
et al., (2004), places Ibla as a sister group to other thoracicans. These results strengthen the
assessment that Ibla is a part of Thoracica. Therefore, the superorder Thoracica Darwin, 1854
now consists of five orders, i.e. Cyprilepadiformes Buckeridge and Newman, 2006; Ibliformes
Buckeridge and Newman, 2006; Lepadiformes Buckeridge and Newman, 2006; Scalpelliformes
Buckeridge and Newman, 2006; and Sessilia Lamarck, 1818.
1.2.2.2 Distribution
Barnacles are abundant, with over 1000 species found in polar, tropical, and temperate
waters worldwide, from the shore to the depths of the ocean (Fertl and Newman, 2009).
They have adjusted to various lifestyles, from parasites of decapod crustaceans to free-living
groups. Barnacles also have one of the best fossil records of the crustacea, extending from
Middle Cambrian Burgess Shale to the Recent era (Newman, 1979; Schram, 1982). The most
abundant number of thoracicans were found in the Mesozoic and Cenozoic and extends back
to the Upper Silurian. Although it appears that cirripeds are an old group, extending well back
in the Palaeozoic, the great radiation of calcareous forms may not have begun until the Triassic
era (Whyte, 1976; Schram, 1982).
As burrowing barnacles, acrothoracicans are generally very small and show their greatest
diversity in coral seas (Tomlinson, 1969). Their fossil record is known from the Late Devonian to
the Recent era, but the only definitely identifiable acrothoracican is Typetesa caveata, in Late
Palaeozoic myalinid clams (Rodriguez and Gutschick, 1977; Schram, 1982). On the other hand,
the distribution of extant acrothoracican is cosmopolitan, with the largest number of species
found in the Indo-West Pacific (Tomlinson 1969, 1973).
Rhizocephalan barnacles are a purely parasitic group that is found exclusively on
crustaceans, primarily decapods. This order is probably the poorest known of the cirripeds,
and has no fossil record (Schram, 1982; Abele, 1982).
As the most abundant Cirripedia, the Thoracica can be found in all marine environments,
from the highest reaches of the tides to the depths of the oceans. Some Thoracica can be
found in estuaries, but none completes its life cycle in freshwater. Their diversity is greatest
in the tropical Indo-Pacific, less in the northeast Pacific, and far less in the North Atlantic
(Cornwall, 1951, 1955; Henry, 1940, 1942; Ross, 1962; Zullo, 1966). There is a latitudinal
pattern in these cirripeds, where the number of species of littoral thoracicans increases with
decreasing latitude (Nilsson-Cantell 1938, 1978; Stubbings, 1967; Newman and Ross, 1971;
General introduction
7
Spivey, 1981). There is also a longitudinal pattern, with the number of species increasing in the
order Eastern Atlantic, Western Atlantic, Eastern Pacific, and Indo-West Pacific, as shown by
the Amphibalanus amphitrite complex (Henry and McLaughlin, 1975).
1.3
Aims and objectives of the thesis
The main goal of this doctoral thesis is to provide an integrative taxonomic approach
of barnacles from the Moluccas, including information on their morphology, distribution,
and substrate. This thesis is divided into three major sections. The first seeks to establish a
taxonomic checklist of barnacles from the Moluccas, eastern Indonesia, including molecular
phylogeny and distribution data from recently collected material and previous records. The
second examines deep-sea barnacle material collected from the Moluccas through the Karubar
expedition (1991), which has been deposited at the Muséum National d’Histoire Naturelle
(MNHN), Paris, but remained unstudied today. The third explores a new method for studying
sponge-dwelling barnacles from the Moluccas, using X-ray micro-computed tomography
(Micro-CT scanning), a modern non-destructive imaging method. This thesis seeks to answer
the following key questions:
•
•
•
•
How many species of Moluccan barnacles were recently collected?
How many were already described? Are there new species or first records?
How are the species from the checklist related? Are the morphologically characterized
species supported by the molecular results?
How many species of deep-sea barnacles were collected from the Moluccas Islands
by the Karubar expedition (1991) and deposited in the Muséum National d’Histoire
Naturelle (MNHN), Paris?
Does an innovative imaging method like X-ray micro-computed tomography (Micro-CT
scanning) allow a non-destructive study of the shell morphology of sponge-inhabiting
barnacles? What are the differences compared to a conventional light microscopy
approach?
This doctoral thesis consists of three research articles (three chapters and one additional
data publication) that were written for and submitted to international peer-reviewed journals
and the MfN Data Repository in Berlin. The first manuscript was published in ZooKeys and the
second one was published in Zoosystematics and Evolution; the third manuscript has been
submitted to Treubia and currently is under review. Chapter 1.4 below provides an overview
of these three manuscripts and one published data repository of micro-CT scanning results.
1.4 Material and methods
1.4.1 Fieldwork
Fieldwork was undertaken during nineteen field trips to the intertidal zones of the
Moluccan islands of Ambon, Saparua, Seram, Pombo, and Banda Neira, conducted between
January 2016 and September 2017. Sampling was carried out at 24 localities in Ambon Island,
8
Chapter 1
12 localities in Saparua Island, 7 localities in Seram Island, 4 localities in Pombo Island, and 2
localities in Banda Island. Additional material for molecular analysis and micro-CT scanning
was collected from the island of Sulawesi between September and October 2017. Access to
the deep-sea barnacle collection of the Muséum National d’Histoire Naturelle (MNHN), Paris,
was undertaken between 1 and 14 September 2019.
Barnacles were collected by directly extracting them from their natural and artificial
substrate in the sampling sites while snorkelling for underwater samples. Barnacle specimens
firmly attached to a hard substrate (rocks, stone, concrete) were sampled using a chisel and
hammer, whereas those attached to the softer substrate were sampled using a craft knife.
The sample of barnacles was then put in a labelled plastic bag, with 96% ethanol added as a
fixative, before being placed in a plastic container. After a day, the ethanol was replaced with
the new ethanol solution. The ethanol replacement was repeated up to three times, before
the specimen was transferred into 75% ethanol for long-term preservation.
1.4.2 Morphological analysis
The specimen of barnacle were determined at the Museum für Naturkunde Berlin using
classical systematics by observing the morphology of its hard parts, such as the shell (parietes)
and the cover shell (opercular plates); and soft body, such as the mouth part (trophy) and the
filter legs (cirri). Observation of the hard parts was done using a stereomicroscope, whereas
the anatomy of the soft body was observed using light microscopy. In this study, classical
systematics was combined with biometric methodology using digital callipers (accurate to 0.1
mm), and many characters were utilised.
Characteristics measured through biometrics were basal length of shell, basal width,
orifice length, orifice width, and carinal height (for acorn barnacles); total height, capitular
height, diameter of the base of the capitulum, carina and scutum distance, scutal length, scutal
width, tergal length and tergal width (for stalked barnacles); capitular height, capitular width,
peduncular length, orifice height, number of crests, capitular thickness, and peduncular width
(for deep-sea barnacles of Heteralepas); the number of teeth on upper lip (labrum), their
average number on both sides of the labrum; the number of teeth or setae on the maxillule,
the average number of spines between the upper and the lower pair; and the number of teeth
on the mandible.
Species identification was conducted according to the classification and description
of the species by Darwin (1852, 1854), Hoek (1883, 1907, 1913), Pilsbry (1890, 1907, 1916),
Annandale (1905, 1906, 1908, 1909, 1910, 1913, 1916), Broch (1916, 1922, 1931), Calman
(1918, 1919), Newman and Ross (1971, 1976), Foster (1974, 1978, 1980, 1981), Rosell (1981,
1989, 1991), Kolbasov (1993), Southward and Newman (2003), Chan et al. (2007), Pérez-Losada
(2008, 2014), Chan et al. (2009, 2010), Shalaeva and Boxshall (2014), Tsang et al. (2015), and
Chan et al. (2017).
General introduction
9
1.4.3 Molecular phylogenetic analysis
Genomic DNA was extracted from the adductor muscle tissue using a CTab isolation
buffer following the method of Doyle and Doyle (1987). This study used two gene fragments,
cytochrome oxidase subunit I (COI) and 18S ribosomal RNA gene (18S). COI was sequenced
using the primers LCO1490 (5’-GGT CAA CAA ATC ATA AAG ATA TTG G-3’) and HCO2198 (5’-TAA
ACT TCA GGG TGA CCA AAA AAT CA-3’) (Folmer et al. 1994), and 18S using the primers ai (5’-CCT
GAG AAA CGG CTA CCA CAT C-3’) and 7R (5’-GCA TCA CAG ACC TGT TAT TGC-3’) (Whiting 2002).
PCR was conducted and the products were sent to Macrogen Europe for cycle sequencing
of both strands of each gene. Chromatograms were edited using CodonCode Aligner version
5.1.5 (http://www.codoncode.com) for COI and Geneious 11 (http://www.geneious.com)
for 18S. All new DNA sequences generated for this study were deposited in GenBank. For
comparison, 84 COI sequences and 88 18S sequences of related barnacles were downloaded
from GenBank. Sequences were aligned using Muscle (Edgar 2004), as implemented in
Geneious and later exported as nexus or fasta files. Phylogenetic trees were reconstructed
for each gene using both Maximum Likelihood (ML) and Bayesian Inference (BI). ML analyses
were conducted using RAxML Black Box (Stamatakis et al. 2008), with 100 bootstrap replicates
and under the GTR + I + G model of sequence evolution. Bayesian analyses were conducted in
BEAST 2. Genetic distances (K2P) were calculated using MEGA version X (Kumar et al. 2018).
All molecular work was done at the Museum für Naturkunde Berlin.
1.4.4 X-ray micro-computed tomography and 3D reconstruction
3D-data sets were obtained via μCT using an FF35 Scanner (Yxlon, Hamburg) at the
Museum für Naturkunde Berlin. Scans were performed with the following parameters: e.g.
80 kV, 15 W, 16 μm voxel size, 2000 projections/turn helical CT. For all of the example data
sets, raw files were produced with the help of the software VGStudio Max (Volume Graphics,
Heidelberg, Germany).
For subsequent processing of the reconstructed 3D-data, the software Amira 6.0.0 was
used. Segmentation was carried out with the magic wand tool to mark the barnacles inside
the sponges. Each segmented barnacle was assigned a different “label”, making it possible
to differentiate between single barnacles by assigning different colours to individual labels.
Switching back to project mode and using the “Volren” volume renderer, the opacity of the
surrounding medium was adjusted so that the barnacles were clearly visible.
1. 5 Overview of the integrated manuscripts
This dissertation is written in a cumulative form and consists of three separate manuscripts
and one published data repository (Chapters 2-4), which have been published in international
peer-reviewed scientific journals (for Manuscript 1 and 2), submitted for publication in such a
journal (for Manuscript 3) and published in the MfN Data Repository Berlin. Table 1.1 provides
the title and authorship, selected scientific journal, and publication status of each manuscript.
Chapter 1
10
Table 1.1: Overview of the three manuscripts presented in this thesis
Chapter
2
Title
Journal
Authorship
Status
An annotated checklist and integrative biodiversity
ZooKeys, Volume 945, pp. 17–83
discovery of barnacles (Crustacea, Cirripedia) from
doi: 10.3897/zookeys.945.39044
the Moluccas, East Indonesia
Pipit Pitriana, Luis Valente, Thomas von Rintelen,
published: 3 July 2020
Diana S. Jones, Romanus E. Prabowo, Kristina von
Rintelen
3
New insights gained from museum collections: Deep-
Zoosystematics and Evolution, Volume 96,
sea barnacles (Crustacea, Cirripedia, Thoracica) in
Issue 2, pp.649–698
the Muséum National d’Histoire Naturelle, Paris,
doi: 10.3897/zse.96.55733
collected during the Karubar expedition in 1991
Pipit Pitriana, Diana S. Jones, Laure Corbari, Kristina
published: 28 September 2020
von Rintelen
4
Exploring sponge-inhabiting barnacles of eastern
Treubia
Indonesia using Micro-CT scanning
Pipit Pitriana, Andreas Wessel, Tina Aschenbach,
submitted: 9 September 2020
Kristina von Rintelen
Non-invasive 3D visualization of the sponge-
Data Publisher: Museum für Naturkunde
inhabiting barnacle Acasta sulcata (Crustacea,
Berlin (MfN) - Leibniz Institute for
Cirripedia, Balanomorpha) from the Moluccas,
Evolution and Biodiversity Science.
Indonesia. [Dataset].
https://doi.org/10.7479/87tp-gr35.
Pipit Pitriana, Kristina von Rintelen, Andreas Wessel.
published: 17 September 2020
1.6 Contribution to the individual manuscripts
Pipit Pitriana is the main author of all manuscripts (chapters 2, 3 and 4) and predominantly
planned, wrote and accomplished them. She collected all samples from the Maluku Islands,
Indonesia and also conducted the research visit to the Muséum National d’Histoire Naturelle
(MNHN), Paris, in order to study deep-sea barnacles collected by the Karubar expedition
(1991). She treated all barnacle samples in the laboratory, identified the taxa, analysed the
samples, took pictures of the specimens, and wrote, finalised, and published the manuscripts.
Pipit Pitriana’s contribution to each manuscript is given in Table 1.2.
Table 1.2: Overview of the author’s contribution to each manuscript.
Chapter/
Manuscript
2/ 1
3/ 2
4/ 3
Contribution of Pipit Pitriana [%]
conception
accomplishment
90
80
90
80
90
90
publication
85
90
90
11
CHAPTER 2
An annotated checklist and integrative biodiversity discovery
of barnacles (Crustacea, Cirripedia) from the Moluccas,
East Indonesia
Pipit Pitriana1,2,6, Luis Valente1,3, Thomas von Rintelen 1, Diana S. Jones4,
Romanus E. Prabowo5, Kristina von Rintelen1
Museum für Naturkunde – Leibniz Institute for Evolution and Biodiversity Science, Invalidenstrasse 43,
10115 Berlin, Germany
2
Research Centre for Deep-sea, Indonesian Institute of Science (LIPI), Jl. Y. Syaranamual, Poka, Tlk.
1
Ambon, Kota Ambon, Maluku, Indonesia
Naturalis Biodiversity Center, Understanding Evolution Group, Postbus 9517, 2300 RA Leiden, the
Netherlands
3
The Western Australian Museum, 49 Kew Street, Welshpool WA 6106, Locked Bag 49, Welshpool DC
WA 6986, Australia
4
Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Indonesia, 53122
Institute of Geological Sciences, Freie Universität Berlin, Malteserstrasse 74–100 Building C and D,
5
6
12249 Berlin, Germany
published in: ZooKeys, Volume 945, pp. 17-83
accepted: 7 April 2020, published: 3 July 2020
This is a post-peer-review, original version of an article published in a peer-reviewed,
open-access journal of ZooKeys. The final authenticated version is available online at:
https://doi.org/10.3897/zookeys.945.39044
2. 1 Abstract
To contribute to the taxonomic knowledge of barnacles in this understudied area,
the first checklist of barnacles from the Moluccas Islands is presented, including additional
information on morphology, distribution, and substrate as well as molecular data. The species
of barnacles from the Moluccas have been determined using morphological analysis and DNA
sequences. During 19 field trips conducted between January 2016 and September 2017, 1,513
specimens of 24 species of intertidal and one species of deep-sea barnacles were collected
from 51 localities from the islands. Morphological and molecular analysis of the collected
material detected members of three families of stalked barnacles and four families of acorn
barnacles. In addition to sampling in the field, we also surveyed the literature on barnacles
12
Chapter 2
from the Moluccas Islands. In total, our checklist comprises 97 species from the Moluccas
including 23 new records, two of them yet to be described species. Results suggest that the
Moluccas have a much higher diversity of barnacles than previously known, for example, from
the reports of Challenger and Siboga expeditions. For further work, routine application of
molecular systematics could aid the detection of cryptic species, while increased sampling of
more islands and a taxonomic revision of several groups would likely lead to an even higher
number of species than currently known.
2. 2 Introduction
Barnacles (Crustacea: Cirripedia) are an ancient, species-rich and abundant group
of crustaceans with about 1,400 extant species (Newman and Abbott 1980). They have a
worldwide distribution in tropical and temperate marine environments and at different depths
and are adjusted to various lifestyles, from parasites of decapod crustaceans to free-living
groups. Most cirripeds usually have two free-swimming planktonic larval stages consisting of
distinctive nauplii and a unique non-feeding cyprid (Darwin 1852, 1854; Pochai et al. 2017). In
the most abundant group, the Thoracica, adult specimens are permanently attached to various
types of substrates, other living organisms (e.g., mangroves, corals, molluscs, other barnacles,
sponges), rocks, and man-made materials such as cargo ships and concrete walls (Newman
and Abbott 1980; Power et al. 2010). The Thoracica comprise the orders Cyprilepadiformes,
Ibliformes, Lepadiformes, Scalpelliformes, and Sessilia (Buckeridge and Newman 2006).
Figure 2.1. Map of the Moluccan Islands (Maluku in grey) in eastern Indonesia. Map modified from
Shorthouse (2010).
Checklist of Cirripedia from the Indonesian Moluccas
13
The Indonesian Moluccas (or Spice Islands; Fig. 2.1) are part of the Coral Triangle, one
of the most complex biogeographical and oceanographic areas on Earth. Although part of
the global epicentre of marine biodiversity, knowledge of the barnacle fauna of the Moluccas
Islands is relatively sparse. The exploration of the natural history of the Moluccas Islands dates
back to the 17th century, starting with Georg Everhard Rumphius, and later, for example, Alfred
Russel Wallace (Strack 1993; Lamoureux 1990). In the 19th and 20th centuries, there were
approximately fifty scientific expeditions passing through or specifically targeting the area, such
as the British Challenger (1872-1876), the Dutch Siboga (1899-1900) and the Snellius (19291930) expeditions (Lamoureux 1990). The most recent being the French Karubar expedition in
1991 (Crosnier et al. 1997).
Rumphius provided the first record of a barnacle (the stalked Mitella Oken, 1815 (=
Capitulum, Gray, 1825) found on a rock near the beach at Ambon Island) in his posthumously
published ‘Amboinsche Rariteitkamer’ (Rumphius 1705). Indonesian and Moluccan barnacles
were also studied by Darwin (1854), who assigned them to one of four geographical ‘provinces’,
the third being the East Indian Archipelago. Moluccan barnacles have not been studied since
Buckeridge (1994) examined some material from the Karubar expedition.
To contribute to the taxonomic knowledge of this understudied area, we herein present
the first checklist of barnacles from the Moluccas Islands, including additional information on
morphology and molecular data, as well as distribution and substrate.
2.3 Material and methods
2.3.1 Sampling
Specimens examined in this study were collected by the first author during 19 field trips
between January 2016 and September 2017 to the intertidal zones of the Moluccan islands
of Ambon, Saparua, Seram, Pombo, and Banda Neira (Fig. 2.1, Table 2.1, Supplementary Table
7.1). Deep-water barnacles (Table 2.1) from the Lifamatola Passage (250 m) and Halmahera Sea
(250 m) were provided by Nurul Fitriya. Additional material used for the molecular analyses
was collected from the island of Sulawesi in September to October 2017 (Supplementary Table
7.1). In total, 159 lots containing 1,513 specimens were collected from 51 Moluccan localities.
Barnacle specimens firmly attached to hard substrate (rocks, stone, concrete) were
sampled using a chisel and hammer whereas those attached to softer substrate using a craft
knife. Specimens were fixed and stored in 96% ethanol and transferred into 75% ethanol for
long-term preservation.
2.3.2 Morphological analyses
For detailed morphological analyses, all samples were studied at the Museum für
Naturkunde in Berlin (ZMB), Germany. All specimens are deposited at the Museum Zoologicum
Bogoriense (MZB; Supplementary Table 7.1), Research Center for Biology, Indonesian Institute
14
Chapter 2
of Sciences – LIPI, Indonesia. Barnacle species attached to other barnacle species were not
separated (except for specimens dissected and measured) but were kept within the same glass
container, enabling further morphological studies of different species attached to each other,
e.g., with MicroCT scans.
Specimens were studied by the first author. All species were determined based on
external shell morphology, including the pattern of the parietes, opercular plates, mouth
parts, and arthropodal characters, as described by Darwin (1852, 1854), Hoek (1907, 1913),
Southward and Newman (2003), Chan et al. (2007), Pérez-Losada (2008, 2014), Chan et al.
(2009a), Tsang et al. (2015), and Chan et al. (2017).
Hard body parts (parietes and opercular plates) were separated from soft body parts
using a scalpel. Shell plates were separated and cleaned with a bleach solution to remove
any organic material, rinsed with fresh water, dried and observed under a stereo microscope
(Leica M125) and photographed with a digital camera (Leica Microsystems M205C and Leica
Z16 APo-A) (Fig. 2.2).
Figure 2.2 External morphology of barnacles showing the two parts of the operculum (tergum and
scutum) and carina (without scale).
The mouthparts (labrum, palps, maxilla, maxillule, and mandible) were dissected using a
scalpel, each was mounted on a glass slide and examined under a light microscope (Axioskop
20). The cirri were separated into couples of cirri I-VI and the penis, before being mounted on
glass slides. The anatomy of these soft body parts was studied using a light microscope.
All measurements were made using digital callipers (accurate to 0.1 mm; Supplementary
Tables 2-26) generally following the method described in Beşir and Çınar (2012): basal length
of shell, basal width, orifice length, orifice width, and carinal height. For stalked cirripeds,
measurement of total height, capitular height, diameter of the base of the capitulum, carina
and scutum distance, scutal length, scutal width, tergal length and tergal width were taken
following the method described by Igić (2007). For deep-water barnacles, capitular height,
capitular width, peduncular length, orifice height, number of crests, capitular thickness, and
peduncular width were taken following the method described by Chan et al. (2009b).
Checklist of Cirripedia from the Indonesian Moluccas
15
2.3.3 Molecular phylogenetic analyses
We performed molecular phylogenetic analyses including new DNA sequences from our
new samples from the Moluccas in combination with sequences of multiple barnacle specimens
retrieved from GenBank. Our aim with the molecular analyses was not to provide a robust
phylogeny of barnacles or to develop DNA barcodes for Moluccan barnacle taxa. Instead, our
goal was to confirm the molecular taxonomic identity of the barnacles from the Moluccas with
the published sequences in the GenBank, to examine whether they cluster near to congeneric
or conspecific accessions. With this exercise we aimed to gain insights into the taxonomic
positions of Moluccan barnacles in addition to those we may gain from morphology.
Genomic DNA was extracted from the adductor muscle tissue using CTAB isolation buffer
following the method of Doyle & Doyle (1987), as described at http://www.geocities.com/
CapeCanaveral/8431/CTAB.html. Tissue was ground and placed in 200 μl CTAB buffer and 5 μl
Proteinase K, homogenized by shaking, and incubated at 56°C overnight. DNA was extracted
from the lysate using a Qiagen BioSprint 96 using the manufacturer’s protocol. The purified
DNA was stored at −20°C until required, and dilutions of 1 to 10 were used for the polymerase
chain reaction (PCR).
We sequenced two gene fragments: cytochrome oxidase subunit I (COI), a rapidly
evolving gene from the mitochondrial genome; and the 18S ribosomal RNA gene (18S), a slowly
evolving and generally highly conserved gene from the ribosome. We chose these markers
because of their contrasting evolutionary rates, but also because they have been widely used
in barnacle phylogenetic analyses (Pérez-Losada et al., 2008, 2014; Tsang et al., 2014, 2015).
COI was sequenced using primers LCO1490 (5’-GGT CAA CAA ATC ATA AAG ATA TTG G-3’)
and HCO2198 (5’-TAA ACT TCA GGG TGA CCA AAA AAT CA-3’) (Folmer et al. 1994), and 18S
using primers ai (5’-CCT GAG AAA CGG CTA CCA CAT C-3’) and 7R (5’-GCA TCA CAG ACC TGT TAT
TGC-3’) (Whiting 2002). PCR was performed in 25 μl volumes containing 17.8 µl ddH2O, 2.5 µl
10 x Puffer, 1 µl Mg (25mM), 0.5 µl dNTP Mix, 0.5 µl of each primer, and 0.2 µl Taq Polymerase
(2.00 units/25 µl PCR) with 2 µl DNA. The reaction conditions of PCR were 94º C for 3 minutes
before 35 cycles of amplification, with 94º C for 30 sec, 55º C for 1 minute and 72º C for 60
sec, followed by a final extension at 72º C for 5 minutes. PCR products were sent to Macrogen
Europe for cycle sequencing of both strands of each gene.
Chromatograms were edited using CodonCode Aligner version 5.1.5 (http://www.
codoncode.com) for COI and Geneious 11 (http://www.geneious.com) for 18S. All new DNA
sequences generated for this study are deposited in GenBank under the accession numbers
provided in Supplementary Table 7.1.
For comparison, 84 COI sequences and 88 18S sequences of related barnacles were
downloaded from GenBank (accession numbers are provided in Figs 2.28-2.29). Sequences
were aligned using Muscle (Edgar 2004) as implemented in Geneious and later exported as
nexus or fasta files.
16
Chapter 2
Phylogenetic trees were reconstructed for each gene using both Maximum Likelihood
(ML) and Bayesian Inference (BI). ML analyses were conducted with RAxML Black Box
(Stamatakis et al. 2008) with 100 bootstrap replicates and under the GTR + I + G model of
sequence evolution. Bayesian analyses were conducted in BEAST 2. As our goal was to obtain
a topology and not dates for the branching events, we used a Bayesian relaxed lognormal
clock with a rate of 1. Therefore, the ages obtained in the ultrametric trees emerging from this
analysis are relative not absolute. Substitution model selection was performed in jModeltest
(Posada 2008) using the Akaike information criterion, and GTR + I + G was identified as the best
model for both genes. For each analysis, we ran two independent chains of between 10 and
40 million generations, with a birth-death tree prior. Convergence of chains and burn-ins were
assessed with Tracer, runs combined using LogCombiner, and maximum clade credibility trees
produced in Tree Annotator.
Genetic distances (K2P) were calculated by MEGA version X (Kumar et al. 2018).
2.4 Results
2.4.1 Checklist tabulation
This study provides the most comprehensive overview of barnacle species from the
Moluccan islands (Table 2.1). The morphological analyses of the collected material revealed
24 intertidal species and one deep-water species from three families of stalked barnacles
(Heteralepadidae: one genus and species; Lepadidae: two genera and species; Pollicipedidae:
one genus and species) and four families of acorn barnacles (Pachylasmatidae: one genus and
species; Chthamalidae: five genera and species; Tetraclitidae: five genera and eight species;
Balanidae: two genera and seven species).
Including previous records from the literature, we found a total of 97 species from the
Moluccan islands (Table 2.1) from the superorder Thoracica (free living or epizoic). Of these, 21
are new records and two (Amphibalanus sp. and Microeuraphia sp.) are currently unidentified
species.
All specimens obtained from field work, except for one floating specimen, were attached
to types of natural and artificial substrates (Table 2.1), and several smaller species, e.g.,
Chthamalus moro, were also attached to other larger barnacle species, e.g., Megabalanus
tintinnabulum. In general, the smallest species was C. moro (basal length: 2.4-5.1 mm; basal
width: 1.4-4.1; height 0.8-1.7 mm), and the largest M. tintinnabulum (basal length: 26.0-49.3
mm; basal width 29.0-43.1 mm; height 20.1-49.4 mm).
Checklist of Cirripedia from the Indonesian Moluccas
17
Table 2.1. Annotated checklist tabulation of barnacle species from the Moluccas, Eastern Indonesia
ORDER
Family
Suborder
Subfamily
LEPADIFORMES
Heteralepadidae
Heteralepadomorpha
Lepadomorpha
No.
Name
Locality
Substrate
Deep-water
cable to
mooring
References
1
Heteralepas japonica
(Aurivillius, 1892)
Lifamatola
Sea and
Halmahera
Sea
2
Heteralepas ovalis
(Hoek, 1907)
West from
Kei Islands
Hoek (1913)
3
Heteralepas tenuis
(Hoek, 1907)
South of
Seram
Hoek (1913)
Oxynaspididae
4
Oxynaspis connectens
Broch, 1931
Kei Islands
Jones and
Hosie (2016)
Poecilasmatidae
5
Glyptelasma
carinatum (Hoek,
1883)
Seram Sea
Hoek (1913)
6
Megalasma striatum
(Hoek, 1883)
East of Kei
Islands
Hoek (1913)
7
Octolasmis orthogonia Tual
Darwin, 1852
anchorage
Hoek (1913)
8
Octolasmis weberi
(Hoek, 1907)
Kei Islands,
Banda Sea
Hoek (1913),
Jones and
Hosie (2016)
9
Poecilasma kaempferi
Darwin, 1852
Banda Sea
Jones and
Hosie (2016)
10 Temnaspis fissum
(Darwin, 1852)
Ternate
anchorage
Hoek (1913)
11 Conchoderma
virgatum Spengler,
1789
Banda Sea
Jones et al.
(2001)
Lepadidae
SCALPELLIFORMES
Genus / Species
This study
12 Dosima fascicularis
Ambon
(Ellis & Solander, 1786)
Floating in water This study
at the beach
13 Lepas anserifera
Linnaeus, 1767
Ambon,
Saparua,
Seram,
Pombo
Mangrove,
This study
stone ship chart
and ship wall,
port pole, shell
of Megabalanus
zebra
14 Lepas pectinata
Spengler, 1793
Banda Sea
Jones and
Hosie (2016)
15 Calantica
pollicipedoides (Hoek,
1907)
East of Kei
Islands
Hoek (1913)
16 Euscalpellum
rostratum (Darwin,
1852)
Kei Islands
Jones and
Hosie (2016)
Pollicipedidae
17 Capitulum mitella
(Linnaeus, 1758)
Ambon,
Saparua
Scalpellidae
Scalpellinae
18 Compressoscalpellum
inflatum (Hoek, 1907)
West of Aru
Island
Calanticidae
Rocks, stone,
wall of fortress,
port pole and
concrete wall
Rumphius
(1705), this
study
Hoek (1913)
18
Chapter 2
ORDER
Family
Suborder
Subfamily
Arcoscalpellinae
Genus / Species
No.
Name
Locality
Substrate
References
19 Scalpellum fissum
Hoek, 1913
West of
Halmahera
Hoek (1913)
20 Scalpellum stearnsi
Pilsbry, 1890
Near Kei
Islands
Hoek (1913)
21 Amigdoscalpellum
vitreum (Hoek, 1883)
South of
Seram,
South of
Ambon
Hoek (1913)
22 Anguloscalpellum
pedunculatum (Hoek,
1883)
Kei Islands
Hoek (1913)
23 Arcoscalpellum
South of
cilliatum (Hoek, 1907) Ambon
Hoek (1913)
24 Arcoscalpellum
discolor (Hoek, 1907)
Banda Sea
Hoek (1913)
25 Arcoscalpellum
Banda Sea
sculptum (Hoek, 1907)
Hoek (1913)
26 Arcoscalpellum
sociabile (Annandale,
1905)
Banda Sea
Jones et al.
(2001)
27 Planoscalpellum
hexagonum
(Hoek, 1907)
Banda Sea
Hoek (1913)
28 Teloscalpellum
imbricatum
(Hoek, 1907)
Near Kei
Islands
Hoek (1913)
29 Trianguloscalpellum
balanoides (Hoek,
1883)
Banda Sea
Hoek (1913),
Shalaeva
and Boxshall
(2014)
30 Trianguloscalpellum
diota (Hoek, 1907)
Near Kei
Islands
Hoek (1913)
31 Trianguloscalpellum
hamulus (Hoek, 1907)
Kei Islands
Hoek (1913)
32 Trianguloscalpellum
Moluccan
hirsutum (Hoek, 1883) Sea
Hoek (1913),
Shalaeva
and Boxshall
(2014)
33 Trianguloscalpellum
indicum (Hoek, 1883)
Banda Sea
Hoek (1913),
Shalaeva
and Boxshall
(2014)
34 Trianguloscalpellum
moluccanum (Hoek,
1883)
Banda Sea;
West of Aru
Island
Hoek (1913),
Shalaeva
and Boxshall
(2014)
35 Trianguloscalpellum
sessile (Hoek, 1907)
Seram Sea
Hoek (1913)
36 Verum candidum
(Hoek, 1907)
Near Kei
Islands
Hoek (1913),
Jones and
Hosie (2016)
Checklist of Cirripedia from the Indonesian Moluccas
ORDER
Family
Suborder
Subfamily
Meroscalpellinae
SESSILIA
Verrucomorpha
Balanomorpha
Verrucidae
Pachylasmatidae
Hexelasmatinae
Pachylasmatinae
Chthamalidae
Notochthamalinae
Euraphiinae
Genus / Species
No.
Name
Locality
Substrate
19
References
37 Annandaleum
japonicum (Hoek,
1883)
Aru Island
Hoek (1913)
38 Annandaleum
laccadivicum
(Annandale, 1906)
Kei Islands
Hoek (1913)
39 Altiverruca navicula
(Hoek, 1913)
Between
Seram
and New
Guinea,
Kei Islands,
Tanimbar
Island
Hoek (1913),
Buckeridge
(1994)
40 Brochiverruca dens
(Broch, 1932)
Tanimbar
Island
Buckeridge
(1994)
41 Cristallinaverruca
cristallina (Gruvel,
1907)
Banda Sea
Jones and
Hosie (2016)
42 Metaverruca recta
(Aurivillius, 1898)
Kei Islands
Hoek (1913)
43 Newmaniverruca
albatrossiana (Pilsbry,
1912)
East of Kei
Islands,
Tanimbar
Island
Hoek (1913),
Buckeridge
(1994)
44 Rostratoverruca
intexta (Pilsbry, 1912)
Kei Islands,
Tanimbar
Island
Hoek (1913),
Buckeridge
(1994)
45 Rostratoverruca
Kei Islands,
kruegeri (Broch, 1922) Tanimbar
Island
Jones et al.
(2001),
Buckeridge
(1994)
46 Verruca capsula Hoek, Between
1913
Seram and
New Guinea
Hoek (1913)
47 Hexelasma arafurae
Hoek, 1913
Kei Islands,
Arafura Sea
Hoek (1913),
Jones and
Hosie (2016)
48 Hexelasma velutinum
Hoek, 1913
Kei Islands
Jones et al.
(2001)
49 Pachylasma
integrirostrum Broch,
1931
Ambon
Jones et al.
(2001)
50 Pseudoctomeris
sulcata (NilssonCantell, 1932)
Ambon
Rocks, shell
of Tetraclita
squamosa
This study
51 Hexechamaesipho
pilsbryi (Hiro, 1936)
Ambon
Rocks
This study
52 Nesochthamalus
intertextus (Darwin,
1854)
Ambon
Stone
This study
53 Europhia hembeli
Conrad, 1837
Ambon
Rocks
This study
20
Chapter 2
ORDER
Family
Suborder
Subfamily
Genus / Species
No.
Name
Locality
Substrate
54 Microeuraphia
Kei Islands
withersi (Pilsbry, 1916)
Chthamalinae
Tetraclitidae
Tetraclitellinae
Tetraclitinae
Newmanellinae
Archaeobalanidae
Archaeobalaninae
55 Microeuraphia sp.
Seram
Island
56 Chthamalus
malayensis Pilsbry,
1916
Arafura Sea
57 Chthamalus moro
Pilsbry, 1916
Ambon,
Saparua,
Seram,
Pombo
References
Jones and
Hosie (2016)
Stone, concrete
wall at port
This study
Jones et al.
(2001)
Mangrove,
stone, port
pole, mol-lusk
shell, shells
of Tetraclita
squamosa,
Tesseropora
rosea, and
Capitulum
mitella
58 Tetraclitella
Banda
(Eotetraclitella)
costata (Darwin, 1854)
This study
Hoek (1913)
59 Tetraclitella divisa
Ambon
(Nilsson-Cantell, 1921)
Concrete wall at This study
port
60 Tetraclitella karandei
Ross, 1971
Ambon
Stone, shells
of Capitulum
mitella and
Euraphia
hembeli
This study
61 Tesseropora rosea
(Krauss, 1848)
Ambon,
Saparua
Stone, mollusc
shell
This study
62 Tetraclita
kuroshioensis Chan,
Tsang & Chu, 2007
Ambon,
Saparua
Rocks, concrete
wall at port
This study
63 Tetraclita squamosa
(Bruguière, 1789)
Ambon,
Saparua
Stone, rocks,
This study
concrete bridge
and wall at port,
shipyard.
64 Yamaguchiella
Banda, Kei
coerulescens (Spengler, Islands,
1790)
Ambon,
Saparua
Stone
Hoek (1913),
Jones and
Hosie (2016),
This study
65 Neonrosella vitiata
(Darwin, 1854)
Ambon,
Banda
Neira,
Saparua
Port pole, reef,
stone
This study
66 Newmanella spinosus
Chan & Cheang, 2016
Ambon
Stone, reef
surface
This study
67 Armatobalanus allium Banda Sea
(Darwin, 1854)
Jones and
Hosie (2016)
68 Armatobalanus cepa
(Darwin, 1854)
Jones and
Hosie (2016)
Aru Island
Checklist of Cirripedia from the Indonesian Moluccas
ORDER
Family
Suborder
Subfamily
Bryozobiinae
Pyrgomatidae
Pyrgomatinae
Balanidae
Amphibalaninae
Genus / Species
No.
Name
Locality
Substrate
21
References
69 Armatobalanus
Banda Sea
quadrivittatus (Darwin,
1854)
Jones and
Hosie (2016)
70 Conopea dentifer
(Broch, 1922)
Kei Islands
Jones et al.
(2001)
71 Conopea navicula
(Darwin, 1854)
Near Damar
Island
(South of
Halmahera)
Hoek (1913)
72 Membranobalanus
cuneiformis
(Hiro, 1936)
Arafura Sea
Jones and
Hosie (2016)
73 Solidobalanus
Banda Sea,
auricoma (Hoek, 1913) Ternate, Kei
Islads
Hoek (1913)
Jones et al.
(2001)
74 Solidobalanus socialis
(Hoek, 1883)
Arafura Sea
Hoek (1913)
75 Striatobalanus
amaryllis (Darwin,
1854)
Arafura Sea
Jones and
Hosie (2016)
76 Striatobalanus
Moluccas
kruegeri (Pilsbry, 1916)
Jones and
Hosie (2016)
77 Striatobalanus tenuis
(Hoek, 1883)
Kei Islands,
Arafura Sea
Hoek (1913),
Jones and
Hosie (2016)
78 Multatria terebratus
(Darwin, 1854)
Kei Islands
Hoek (1913),
Jones and
Hosie (2016)
79 Eoatria
Banda Sea,
quinquevittatus (Hoek, Ambon
1913)
Island
Jones and
Hosie (2016)
80 Cantellius
euspinulosum (Broch,
1931)
Ambon
Jones and
Hosie (2016)
81 Cantellius gregarious
(Sowerby, 1823)
Banda Sea
Jones et al.
(2001)
82 Cantellius pallidus
(Broch, 1931)
Banda Sea
Jones et al.
(2001)
83 Galkinius indica
(Annandale, 1924)
Kei Islands
Jones and
Hosie (2016)
84 Hoekia fornix Ross &
Newman, 1995
Moluccas
Jones and
Hosie (2016)
85 Nobia grandis
Sowerby, 1839
Kei Islands
Jones et al.
(2001)
86 Pyrgoma kuri Hoek,
1913
Kei Islands
Hoek (1913)
87 Amphibalanus
amphitrite (Darwin,
1854)
Ambon,
Saparua
Stone, mollusc This study
shell, capitulum
of Lepas
anserifera
22
Chapter 2
ORDER
Family
Suborder
Subfamily
Balaninae
Megabalaninae
Genus / Species
No.
Name
Locality
Substrate
References
88 Amphibalanus
reticulatus (Utinomi,
1967)
Ambon
Stone, concrete
wall at port
This study
89 Amphibalanus
variegatus (Darwin,
1854)
Ambon,
Saparua
Stone, plastic
This study
90 Amphibalanus
zhujiangensis (Ren,
1989)
Ambon,
Saparua,
Seram
Stone,
This study
capitulum of
Lepas anserifera
91 Amphibalanus sp.
Ambon,
Seram
Stone, concrete
wall at port
92 Balanus arcuatus
Hoek, 1913
Banda
Hoek (1913)
93 Balanus hystrix Hoek,
1913
Ambon
Hoek (1913)
94 Balanus longirostrum
Hoek, 1913
Bacan
Hoek (1913)
95 Megabalanus occator
(Darwin, 1854)
Near
Obilatu
Island
Coral
Kolosváry
(1950)
96 Megabalanus
tintinnabulum
(Linnaeus, 1758)
Ambon,
Saparua
Conrete bridge
at port, stone,
reef surface
This study
97 Megabalanus zebra
(Darwin, 1854)
Ambon
Stone,
This study
capitulum of
Lepas anserifera
This study
Systematic account of the 25 species morphologically examined for this
study
Class Hexanauplia Oakley, Wolfe, Lindgren & Zaharoff, 2013
Subclass Thecostraca Gruvel, 1905
Infraclass Cirripedia Burmeister, 1834 (= Cirrhipèdes Lamarck, 1806)
Superorder Thoracica Darwin, 1854
Order Lepadiformes Buckeridge & Newman, 2006
Suborder Heteralepadomorpha Newman, 1987
Family Heteralepadidae Nilsson-Cantell, 1921
Genus Heteralepas Pilsbry, 1907
Heteralepas japonica (Aurivillius, 1892)
Figure 2.3a-g, Table 2.1: species no. 1
Alepas japonica Aurivillius, 1892: 125: Aurivillius 1894: 28, pl. II figs 14, 15, pl. VIII figs 3, 7, pl. IX fig. 3.
Alepas indica Gruvel, 1901: 259: Gruvel 1905a: 162, fig. 179.
Heteralepas (Heteralepas) japonica: Pilsbry 1907a: 101.
Checklist of Cirripedia from the Indonesian Moluccas
23
Heteralepas (Heteralepas) japonica var. alba Krüger, 1911a: 34, pl. 1 fig. 2b.
Heteralepas (Heteralepas) dubia Broch, 1922: 288, fig. 38.
japonica: Pilsbry 1911a: 71, fig. 4; Zevina et al. 1992: 31, fig. 19; Chan et al. 2009a: 61;
Chan et al. 2009b: 88-91, figs 2A-D, 3A-D, 4, 5.
Material examined:
Deep-Sea: 32 specimens, MZB Cru Cir 050, Stn.23 Mooring Lifamatola, 250 m, coll. N. Fitriya,
9 Nov 2016; 13 specimens, MZB Cru Cir 051, Stn.39 Mooring Halmahera Seas, 250 m, coll. N.
Fitriya, 13 Nov 2016; 27 specimens, MZB Cru Cir 052, Stn.56 MO2, 200 m, coll. N. Fitriya, 23
Nov 2016; 42 specimens, MZB Cru Cir 053, Stn.58 MO3, 250 m, coll. N. Fitriya, 25 Nov 2016.
GenBank accession numbers: COI gene (MK995372), 18S (MK981386).
Diagnosis:
Capitulum rounded without hard valves and opercular plates, wall of capitulum tick with crest
not more than two on the carinal region; cirrus I with filamentary appendage at the basal
region; anterior rami shorter than posterior rami in cirri V-VI; caudal appendage present;
maxillule strongly notched.
Description:
Orifice slightly protuberant, crenulated, occupying one half to one third capitular length, parallel to
or at oblique angle to capitulum; integument thick, chitinous; carinal margin sometimes with warty
protuberances on slight keel; peduncle naked; colour of capitulum and peduncle yellowish (Fig.
2.3a-c). Cirrus I with anterior rami (19-segmented) shorter than posterior rami (25-segmented) and
a filamentary appendage present at the basal region (Fig. 2.3d); cirri II - IV long, slender, anterior rami
of cirri V and VI shorter than posterior rami; cirrus VI has a caudal appendage with 20-segmented
and one fourth length of anterior ramus (Fig. 2.3e). Mandible with four large teeth excluding inferior
angle (Fig. 2.3f); maxillule strongly notched with two big teeth on upper angle and blade-shaped
setae on cutting margin (Fig. 2.3g); labrum concave, teeth numerous. Ranges of height of capitulum
11.9-18.6 mm, width 8.7-15.3 mm, thickness 7.0-13.3 mm; length of peduncle 7.7-27.6 mm and
width 5.1-10.4 mm (measurements for 25 specimens are presented in Supplementary Table 7.2).
Distribution:
Heteralepas japonica is widely distributed in Indo-west Pacific: Indian Ocean; Australia; Singapore,
Malacca Str., Indonesia; Malay Archipelago; Vietnam; Condor Island; S China Sea; E China Sea;
Taiwan, Philippines; S Japan; NE New Zealand; fouling hard rock substrata, crabs, gorgonians,
antipatharians, deep-sea cables; 48–500 m (Jones and Hosie 2016). In this study, Heteralepas
japonica was found attached to cable moorings in Lifamatola Sea and Halmahera Sea (a map with
the occurrence of Heteralepas japonica in the Moluccas is shown in Supplementary Fig. 7.1).
Remarks:
The external appearance of this species is extremely variable (Nilsson-Cantell 1927). After an
extensive study of H. japonica, Nilsson-Cantell could not distinguish H. japonica and H. indica
(Gruvel, 1901) and placed the latter in synonymy with H. japonica, and later authors have
24
Chapter 2
followed this suggestion (e.g., Broch 1931, Utinomi 1958). At the same time, Nilsson-Cantell
(1927) also suggested that H. nicobarica Annandale, 1909, H. gigas Annandale, 1905 and H.
cygnus Pilsbry, 1907 could be invalid species and future revision may synonymize some or all
of them. Zullo & Newman (1964) pointed out the uncertainty surrounding the status of several
of the species assigned to Heteralepas due to a lack of zoogeographic and morphological
data, since extensive collections are unavailable. Furthermore, Foster (1978) suggested that
a revision of the genus was called for since the variability of H. japonica, as noted by Foster
and tabulated by Nilsson-Cantell (1927), encompasses characters which have been used to
distinguish several different species by other authors (e.g., H. dubia Broch, 1922, H. cornuta
Darwin, 1852 , H. indica Gruvel, 1901, H. lankestri Gruvel, 1900).
Figure 2.3. Heteralepas japonica (Aurivillius, 1892) (MZB Cru Cir 050-2). (a) Side view showing the capitulum
and peduncle, (b) upper view showing the capitulum and peduncle, (c) side view showing the opening of
operculum, (d) cirrus I showing the filamentary appendage at the basal region, (e) cirrus VI showing the
caudal appendage, (f) mandible, (g) maxillule. Scale bars: a-c) 5 mm, d) 2 mm, e) 3 mm, f-g) 0.5 mm.
Suborder Lepadomorpha Pilsbry, 1916
Family Lepadidae Darwin, 1852
Genus Dosima Gray, 1825
Checklist of Cirripedia from the Indonesian Moluccas
25
Dosima fascicularis (Ellis and Solander, 1786)
Figure 2.4a-c, Table 2.1: species no. 12
Lepas fascicularis Ellis and Solander, 1786: 197, tab. 15 fig. 6; Darwin 1852: 92, pl. 1 fig. 6.
Lepas fascicularis aurivillii Nilsson-Cantell, 1921: 238, fig. 40b.
Lepas cygnea Spengler, 1790: pl. 6 fig. 8.
Pentalasmis spirulicola, P. donovani Leach, 1818: 413.
Pentalasmis fascicularis: Brown 1844: pl. 51 fig. 2.
Lepas fasciculatus: Pilsbry 1907: 81, pl. IX fig.6.
Lepas (Dosima) fascicularis: Weisbord 1979: 28, pl. 2 figs 10-11; Jones et al. 1990: 8.
Dosima fascicularis: Gray 1825: 100; Zevina 1982: 21, fig. 11.
Material examined:
Ambon Island: 19 specimens, MZB Cru Cir 048, Tial, 3°38’10.2”S 128°20’46.9”E, coll. Adin, 19
Sep 2017.
GenBank accession numbers: COI gene (MK995371), 18S (MK981385).
Diagnosis:
The only pelagic barnacle with its own gas-filled float; plates very thin and paper-like; carina
angle bent with a prominent umbo and expanded basal disk; cirri acanthopod.
Description:
Five capitular plates, white, thin, delicate, wide interspaces between dark purple; base of carina
almost round, not imbedded in membrane, distinct angle formed at sub-central carinal umbo
peduncle short, naked (Fig. 2.4a); five filamentary appendages located at base of cirri on each
side of body; caudal appendages small, smooth, summits rounded; mandible with five teeth
(Fig. 2.4c); penis hirsute. Ranges of diameter of capitulum base 4.0-6.8 mm; capitular height
10.5-17.1 mm; total height 11.7-19.5 mm; scutal width 5.4-7.3 mm; scutal length 8.1-11.1
mm; tergal width 2.7-4.4 mm; tergal length 6.2-11.1 mm (measurements for six specimens are
presented in Supplementary Table 7.3).
Distribution:
Dosima fascicularis is cosmopolitan in tropical and temperate seas (Jones and Hosie 2016).
While it has been reported found at New Zealand, South Africa and South America (Newman
and Ross 1971). In the present study, D. fascicularis was found at Ambon Island at Tial (floating
in water at the beach) (a map with the occurrence of Dosima fascicularis in the Moluccas is
shown in Supplementary Fig. 7.2).
Remarks:
Dosima fascicularis is the only pelagic barnacle that produces its own gas-filled float enabling
it to sustain itself on the sea surface (Weisbord 1979). Dosima can also be distinguished from
members of the genus Lepas by the distinct angle formed at the sub-central umbo of the
carina, and by very thin and brittle plates (Hinojosa et al. 2006).
26
Chapter 2
Figure 2.4. Dosima fascicularis (Ellis and Solander, 1786) (MZB Cru Cir 048-19). (a) Side view showing
the capitulum and peduncle, (b) maxillule, (c) mandible. Scale bars: a) 5 mm, b-c) 0.5 mm.
Genus Lepas Linnaeus, 1758
Subgenus Anatifa Bruguière, 1789
Lepas anserifera Linnaeus, 1767
Figure 2.5a-n, Table 2.1: species no.13
Anatifa striata Bruguière, 1789: pl. 166 fig. 3.
Pentalasmis anseriferus: Brown 1844: pl. 51 fig. 1.
Lepas anserifera Linnaeus, 1767: 1109; Darwin 1852: 81, pl. 1 fig. 4; Hoek 1907: 2; Hiro 1937a: 57, fig.
48; Utinomi 1949: 20; Stubbings 1967: 237; Newman 1971: 32, fig. 1; Dong et al. 1982: 73; Zevina
et al. 1992: 14, fig. 6; Igic 2007: 37, fig. 10; Chan et al. 2009a: 45, fig. 34; Keable and Reid 2015: 266.
Material examined:
Ambon Island: 5 specimens, MZB Cru Cir 056, Galala, 3°41’22.2”S 128°10’52.6”E, coll.
P. Pitriana & D. Tala, 6 Sep 2016; 8 specimens, MZB Cru Cir 057, Laha, 3°43’22.5”S
128°05’02.5”E, coll. P. Pitriana & D. Tala, 5 Sep 2016; 74 specimens, MZB Cru Cir 058,
Suli, 3°37’02.0”S 128°16’31.6”E, coll. Adin, 19 Sep 2017; 93 specimens, MZB Cru Cir 059,
Tial, 3°38’10.2”S 128°20’46.9”E, coll. Adin, 19 Sep 2017.
Pombo Island: 5 specimens, MZB Cru Cir 060, Pombo, 3°31’55.5”S 128°22’28.8”E, coll. P.
Pitriana & D. Tala, 8 Sep 2016.
Checklist of Cirripedia from the Indonesian Moluccas
-
-
27
Seram Island: 13 specimens, MZB Cru Cir 061, Lepas Pantai Kawa, 2°57’32.5”S
128°05’33.4”E, coll. P. Pitriana & D. Tala, 19 Sep 2017; 10 specimens, MZB Cru Cir 062,
Desa Murnaten, 2°51’48.8”S 128°20’32.3”E, coll. P. Pitriana & D. Tala, 20 Sep 2017; 10
specimens, MZB Cru Cir 063, Desa Kasie, 2°51’05.5”S 128°32’54.1”E, coll. P. Pitriana &
D. Tala, 20 Sep 2017; 10 specimens, MZB Cru Cir 064, Dermaga Pelita Jaya, 3°00›13.5»S
128°07›09.2»E, coll. P. Pitriana & D. Tala, 21 Sep 2017.
Saparua Island: 19 specimens, MZB Cru Cir 065, Negeri Mahu, 3°31’52.9”S 128°41’12.4”E,
coll. P. Pitriana & D. Tala, 11 Apr 2016.
GenBank accession numbers: COI gene (MK995373 - MK995375), 18S (MK981387 MK981388).
Diagnosis:
Capitulum with five completely calcified plates; surfaces striated with radiating lines; scuta
with conspicuous growth lines; scutal margin of terga without notch, occluding margin of
scutum strongly convex and swollen; carina apex extending to tergum, base of carina forked;
filamentary appendages and caudal appendage present.
Description:
Five capitular plates, closely approximate, white, slightly furrowed, terga sometimes strongly
pectinated, occluding margin arched, protuberant (Fig. 2.5a, b). Scutum with well developed,
strong internal umbonal tooth, left internal umbonal tooth small, or mere ridge; carina produced
below base of scutum as fork, apex pointed (Fig. 2.5c-f); 5-6 filamentary appendages on each
side, one on side of prosoma below base of pedicel of cirrus I, four others placed in pairs beneath
basal segment of pedicel of cirrus I, lowest posterior filament of the four generally larges (Fig.
2.5g); caudal appendages small, smooth, curved, claw-like, tip pointed; mandible with five teeth
excluding inferior angle, lower angle pectinate (Fig. 2.5j), labrum concave, toothed (Fig. 2.5l-m).
Basal diameter of capitulum 2.0-7.2 mm; capitular height 8.1-14.8 mm; total height 14.2-31.9 mm.
Scutal width 4.7-11.3 mm; scutal length 6.3-12.5 mm. Tergal width 4.7-11.3 mm; tergal length 4.39.6 mm (measurements for 25 specimens are presented in Supplementary Table 7.4).
Distribution:
Lepas anserifera is a cosmopolitan, pelagic species occurring in tropical and temperate oceans
(Jones et al. 2001). In this study, Lepas anserifera was found on the islands of Ambon (at Suli,
Tial, Galala, Laha), Pombo, Seram (at Lepas Pantai Kawa, Desa Murnaten, Desa Kasie, Dermaga
Pelita Jaya), and Saparua (at Negeri Mahu). Lepas anserifera was found attached to mangroves,
stone ship charts and ship walls, port poles, and shells of Megabalanus zebra (a map with the
occurrence of Lepas anserifera in the Moluccas is shown in Supplementary Fig. 7.3).
Remarks:
Lepas anserifera can be easily recognized by the presence and positions of the 5-6 filamentary
appendages and the curved caudal appendages (Igić 2007).
28
Chapter 2
Figure 2.5. Lepas anserifera Linnaeus, 1767 (MZB Cru Cir 058-2). (a) Side view showing the capitulum
and peduncle, (b) side view showing the carina, (c) external view of scutum, (d) internal view of scutum,
(e) external view of tergum and carina, (f) internal view of tergum and carina, (g) cirrus I, (h) maxilla, (i)
maxillule, (j) mandible, (k) mandibular palp, (l) labrum, (m) close up view on the teeth of labrum, (n)
penis. Scale bars: a-b) 4 mm, c-f) 3 mm, g, n) 1 mm, h-m) 0.5 mm.
Order Scalpelliformes Buckeridge & Newman, 2006
Suborder Scalpellomorpha Newman, 1987
Family Pollicipedidae Leach, 1817
Checklist of Cirripedia from the Indonesian Moluccas
29
Genus Capitulum Gray, 1825
Capitulum mitella (Linnaeus, 1758)
Figure 2.6a-l, Table 2.1: species no.17
Lepas mitella Linnaeus, 1758: 668.
Pollicipes mitella: Sowerby 1833: fig. 2; Darwin 1852: 316, pl. VII fig. 3; Utinomi 1970: 339; Dong et al.
1982: 69; Zevina et al. 1992: 37, fig. 23.
Polylepas mitella: Blainville 1824: pl. 1 fig. 5.
Mitella mitella: Pilsbry 1907: 6; Annandale 1916: 128, pl. 12 fig. 1.
Capitulum mitella: Gray 1825: 101; Foster 1980: 209; Chan et al. 2009a: 85, fig. 70; Williamson 2014:
758, fig. 1D.
Material examined:
Ambon Island: 10 specimens, MZB Cru Cir 023, Liang, 3°30’13.3”S 128°20’34.1”E, coll.
P. Pitriana & D. Tala, 30 Aug 2016; 2 specimens, MZB Cru Cir 024, Liang, 3°30’13.3”S
128°20’34.1”E, coll. P. Pitriana & D. Tala, 7 Sep 2016; 2 specimens, MZB Cru Cir 025,
Alang, 3°45’11.0”S 128°01’23.1”E, coll. Adin, 20 Sep 2017; 4 specimens, MZB Cru Cir
026, Asilulu, 3°40’50.4”S 127°55’27.6”E, coll. Adin, 20 Sep 2017; 3 specimens, MZB Cru
Cir 027, Dermaga Tulehu, 3°35’21.8”S 128°20’02.8”E, coll. P. Pitriana & D. Tala, 7 Sep
2017; 15 specimens, MZB Cru Cir 028, Tawiri, 3°42’10.1”S 128°06’13.4”E, coll. P. Pitriana
& D. Tala, 29 Mar 2016; 5 specimens, MZB Cru Cir 029, Tawiri, 3°42’10.1”S 128°06’13.4”E,
coll. P. Pitriana & D. Tala, 5 Sep 2016; 2 specimens, MZB Cru Cir 030, Laha, 3°43’22.5”S
128°05’02.5”E, coll. P. Pitriana & D. Tala, 5 Sep 2017; 6 specimens, MZB Cru Cir 031,
Morella, 3°31’06.5”S 128°13’18.0”E, coll. Adin, 20 Sep 2017; 5 specimens, MZB Cru Cir
032, Ureng, 3°40’14.0”S 127°56’47.6”E, coll. Adin, 20 Sep 2017; 5 specimens, MZB Cru
Cir 033, Wakasihu, 3°46’27.6”S 127°56’36.6”E, coll. Adin, 20 Sep 2017.
Saparua Island: 10 specimens, MZB Cru Cir 034, Teluk Saparua, 3°34’25.7”S 128°39’25.8”E,
coll. P. Pitriana & D. Tala, 8 Apr 2016; 11 specimens, MZB Cru Cir 035, Benteng Durstede,
3°34’32.8”S 128°39’34.7”E, coll. P. Pitriana & D. Tala, 8 Apr 2016.
Genbank accession number: 18S (MK981390).
Diagnosis:
Capitulum with more than 18 plates, all with apical umbones; lateral plates numerous and
only one big plate under the rostrum; scales of the peduncle symmetrically arranged in close
whorls.
Description:
Capitulum fan-shaped, with eight large plates, basal ring of 18-25 smaller plates, all plates
yellowish, umbos apical; peduncle covered by numerous yellowish, fine scales (Fig. 2.6a, b);
mandible with five teeth (Fig. 2.6j); labrum concave, teeth lacking (Fig. 2.6l). Capitular diameter
base ranges 4.5-20.4 mm; total height 10.7-47.1 mm; capitular height 7.0-24.6 mm. Ranges of
distance from rostrum to carina 5.8-27.3 mm and rostral height 5.5-24.2 mm (measurements
for 25 are presented in Supplementary Table 7.5).
30
Chapter 2
Figure 2.6. Capitulum mitella (Linnaeus, 1758) (MZB Cru Cir 026-4). (a) Side view showing the capitulum
and peduncle, (b) side view showing the rostrum, (c) external view of scutum, (d) internal view of
scutum, (e) external view of tergum, (f) internal view of tergum, (g) cirrus I, (h) maxilla, (i) maxillule,
(j) mandible, (k) mandibular palp, (l) labrum. Scale bars: a-b) 6 mm, c-f) 2 mm, g) 1 mm, h-l) 0.5 mm.
Distribution:
Darwin (1852) reported Capitulum mitella from the Philippine Archipelago, Ambon, East Indian
Archipelago and Madagascar. Chan et al. (2009) and Jones & Hosie (2016) reported the species
as widely distributed in warmer parts of the Indo-Pacific region, from Madagascar to southern
Japan. In this study, C. mitella was found on the islands of Ambon (at Ureng, Alang, Dermaga
Checklist of Cirripedia from the Indonesian Moluccas
31
Liang, Asilulu, Doc. Tawiri, Morella, Wakasihu, Laha, and Tulehu) and Saparua (at Benteng
Duurstede and Teluk Saparua). Capitulum mitella attach on rocks, stone, wall of fortress, port
pole and concrete wall (a map with the occurrence of Capitulum mitella in the Moluccas is
shown in Supplementary Fig. 7.4).
Remarks:
Capitulum mitella is the famous Japanese goose barnacle or ‘kame-no-te’ (meaning the hand
of the turtle, referring to its shape). This barnacle is edible and sold as an expensive seafood
in Japan, China, Taiwan, and Korea, as well as in Portugal and Spain, where it is known as
‘percebes’.
Order Sessilia Lamarck, 1818
Suborder Balanomorpha Pilsbry, 1916
Family Pachylasmatidae Utinomi, 1968
Subfamily Pachylasmatinae Utinomi, 1968
Genus Pseudoctomeris Poltarukha, 1996
Pseudoctomeris sulcata (Nilsson-Cantell, 1932)
Figure 2.7a-h, Table 2.1: species no. 50
Octomeris sulcata Nillson-Cantell, 1932: 8; Newman & Ross 1976: 40.
Pseudoctomeris sulcata: Poltarukha 1996: 988; Chan et al. 2009a: 156, fig. 131.
Material examined:
Ambon Island: 4 specimens, MZB Cru Cir 073, Leahari, 3°42’45.3”S 128°16’16.5”E, coll. P.
Pitriana, 14 Jan 2016; 2 specimens, MZB Cru Cir 074, Hatu, 3°43’52.7”S 128°02’51.4”E, coll.
Adin, 20 Sep 2017.
Diagnosis:
Shell with eight plates; compound rostrum; scutum and tergum fused; mandible tridentate;
multi-jointed caudal appendage present.
Description:
Shell externally white, internally black; eight plated, rostrum partially fused with rostrolaterals
giving external appearance of six plates (Fig. 2.7a-b); basis membranous, calcareous; orifice
rhomboidal; scutum and tergum thick (Fig. 2.7c, d); maxilla triangular, maxillule not notched
(Fig. 2.7g), mandible with three teeth (Fig. 2.7h), labrum concave, with blunt teeth; cirrus VI
with long, multi-segmented caudal appendages. Ranges of basal length 16.2-16.7 mm, basal
width 11.5-15.0 mm, height 7.4-7.7 mm. Orifice length 5.5-7.9 mm, orifice width 4.3-6.1 mm
(measurements for two specimens are presented in Supplementary Table 7.6).
32
Chapter 2
Distribution:
Pseudoctomeris sulcata was previously recorded from southern Japan, China, and Taiwan (Jones
et al. 2001; Poltarukha and Zevina 2006). In this study, P. sulcata was found on Ambon Island
at Leahari and Hatu on rocks and shells of Tetraclita squamosa (a map with the occurrence of
Pseudoctomeris sulcata in the Moluccas is shown in Supplementary Fig. 7.2).
Figure 2.7. Pseudoctomeris sulcata (Nilsson-Cantell, 1932) (MZB Cru Cir 073-4). (a) upper view, (b) side
view, (c) external view of scutum and tergum, (d) internal view of scutum and tergum, (e) cirrus I, (f)
cirrus III, (g) maxillule, (h) mandible. Scale bars: a-b) 4 mm, c-d) 2 mm, e) 1 mm, f) 2 mm, g-h) 0.5 mm.
Remarks:
Externally, the fused rostrum and rostrolaterals are six-plated, but the sutures are visible
internally (Poltarukha 1996). Morphologically, Pseudoctomeris sulcata shows features of the
Checklist of Cirripedia from the Indonesian Moluccas
33
scutum and tergum similar to those of representatives of the family Pachylasmatida. However,
the species can be distinguished by its tridentate mandible and the presence of multi-jointed
caudal appendages (Poltarukha 2006). A previous molecular study showed that P. sulcata
clustered together with members of the family Pachylasmatidae, not with members of the
Chthamalidae (Chan et al. 2017). According to Chan et al. (2017), P. sulcata is an intertidal
species of the Pachylasmatidae, previously believed to be an exclusive deep-sea taxon.
Superfamily Chthamaloidea Darwin, 1854
Family Chthamalidae Darwin, 1854
Subfamily Notochthamalinae Foster & Newman, 1987
Genus Hexechamaesipho Poltarukha, 1996
Hexechamaesipho pilsbryi (Hiro, 1936)
Figure 2.8a-f, Table 2.1: species no. 51
Chthamalus pilsbryi Hiro, 1936: 227, fig. 3.
Euraphia pilsbryi: Newman & Ross, 1976: 41.
Hexechamaesipho pilsbryi: Poltarukha 1996: 989; Poltarukha 2006: 73-74; Chan et al. 2008: 320, fig. 3;
Chan et al. 2009a: 149, fig. 125; Tsang et al. 2013: 188.
Material examined:
Ambon Island: 20 specimens, MZB Cru Cir 054, Hila, 3°34’57.5”S 128°05’31.9”E, coll. Adin, 20 Sep
2017; 1 specimen, MZB Cru Cir 055, Hatu, 3°43’52.7”S 128°02’51.4”E, coll. Adin, 20 Sep 2017.
Diagnosis:
Shell with six plates; surface grey with black spots scattered; scutum and tergum deeply
interlock forming a sinuous line; cirri I and II with multi-cuspid setae.
Description:
Surface of parietes grey or light brown in colour and spotted with black; orifice rhomboidal
(Fig. 2.8a); basis calcareous; scutum and tergum strongly articulated, forming sinuous line;
scutum elongated, triangular, tergal margin strongly articulated tergum narrow, basi-scutal
angle almost 90˚ (Fig. 2.8b-c); cirrus II with multi-cuspid setae; mandible with three large teeth
(Fig. 2.8f), labrum with row of large teeth. Basal length 8.9-17.0 mm, basal width 10.3-16.4
mm, height 1.0-3.7 mm. Orifice length 4.2-6.9 mm, orifice width 3.6-5.6 mm (measurements
for ten specimens are presented in Supplementary Table 7.7).
Distribution:
Previously, Hexechamaesipho pilsbryi was reported from Japan (Honshu, Shimoda, Wakayama,
Okinawa); Taiwan (Turtle Island, Da Xiang Lang, Shi Ti Ping, Kenting); Philippines (Puerto Galera,
Tiwi-Bicol, Boracay); Malaysia (Nexus Beach, Kota Kinnabalu, Sabah) (Tsang et al. 2013). In the
present study, H. pilsbryi was collected from Hatu and Hila on Ambon Island (a map with the
occurrence of Hexechamaesipho pilsbryi in the Moluccas is shown in Supplementary Fig. 7.2).
34
Chapter 2
A previous study of H. pilsbryi indicated that the distribution of this species bridges the
junction of the Japan region and the Indo-Polynesian province of Briggs (Briggs 1974). Molecular
results of H. pilsbryi analysed by Tsang et al. (2013) suggested that this species can be divided
into two highly diverged lineages: (1) a northern lineage, predominantly distributed in Japan
and Okinawa, and (2) a southern lineage, primarily distributed in Taiwan and Southeast Asia.
Assuming that we have molecular data of H. pilsbryi in our samples, there is a probability that
our samples from the Moluccas include members of the southern lineage.
Remarks:
Hexechamaesipho pilsbryi was first identified from Japan as Chthamalus pilsbryi Hiro, 1936.
However, due to the presence of three large teeth on the mandible, a characteristic of the
subfamily Euraphiinae, the species was placed in the genus Euraphia (Nilsson-Cantell 1921).
Later, due to the presence of multicuspidate setae on cirrus II, Poltarukha (1996) moved E.
pilsbryi to the sub-family Notochthamalinae and determined a new genus, Hexechamaesipho,
which had six parietes and a deeply interlocking scutum and tergum. Currently, H. pilsbryi is
the only species in the genus.
Figure 2.8. Hexechamaesipho pilsbryi (Hiro, 1936) (MZB Cru Cir 055). (a) Upper view, (b) external view
of scutum and tergum, (c) internal view of scutum and tergum, (d) maxilla, (e) maxillule, (f) mandible.
Scale bars: a) 4 mm, b-c) 2 mm, d-f) 0.5 mm.
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35
Genus Nesochthamalus Foster & Newman, 1987
Nesochthamalus intertextus (Darwin, 1854)
Figure 2.9a-h, Table 2.1: species no. 52
Chthamalus intertextus Darwin, 1854: 467, pl. 19 figs 1a, b; Dong et al. 1982: 82; Pope 1965: 29, pl. I
figs 1f, 3a-d.
Euraphia intertextus: Newman & Ross 1976: 41; Zevina et al. 1992: 79, fig. 53.
Nesochthamalus intertextus: Foster & Newman 1987: 326, fig. 3; Southward et al. 1998: 120, fig. 1D,
1H; Chan et al. 2009a: 147, fig. 124.
Material examined:
Ambon Island: 5 specimens, MZB Cru Cir 070, Laha, 3°43’22.5”S 128°05’02.5”E, coll. P. Pitriana
& D. Tala, 7 Sep 2016; 5 specimens, MZB Cru Cir 071, Hila, 3°34’57.5”S 128°05’31.9”E, coll.
Adin, 20 Sep 2017.
GenBank accession numbers: COI gene (MK995376), 18S (MK981389).
Diagnosis:
Shell depressed with large diamond-shaped orifice; scutum and tergum fused; external radii
consist of oblique laminae arising on both sides of the sutures, standing nearly parallel to the
parietes, interfolding with each other; cirri II and III with multi-cuspid setae.
Description:
Shell with six plates, oval, flattened, colour of external shell white to pale grey, interior of shell
violet; orifice rhomboidal; parietal sutures with conspicuous interlocking pattern (Fig. 2.9a,
b); basis membranous with partial secondary calcification with age; opercular plates fused but
separable (Fig. 2.9c, d); cirrus I with rami unequal; mandible with three large teeth (Fig. 2.9e),
mandibular palp with long setae on exterior basal margin (Fig. 2.9f); labrum strongly dentate
(Fig. 2.9g). Basal length 8.9-12.1 mm, basal width 6.9-10.3 mm, height 1.3-3.1 mm. Orifice
length 3.0-4.9 mm, orifice width 2.6-3.9 mm (measurements for ten specimens are presented
in Supplementary Table 7.8).
Distribution:
Nesochthamalus intertextus is known from islands in the West and Central Pacific Ocean - Indonesia,
New Guinea, Malaysia to Vietnam; China; Taiwan; Philippines; Japan; Hawaii; Pitcairn I (Pope 1965;
Newman and Ross 1976; Chan et al. 2009; Jones and Hosie 2016). In this study, N. intertextus was
found on Ambon Island at Laha and Hila on stone (a map with the occurrence of Nesochthamalus
intertextus in the Moluccas is shown in Supplementary Fig. 7.2).
Remarks:
Nesochthamalus intertextus can be distinguished by the conspicuous interlocking pattern
exhibited by the parietal sutures and features of the basis, which is membranous in young
specimens but becomes secondarily calcified with age, leaving a membranous centre only
(Poltarukha 2008; Pope 1965).
36
Chapter 2
Figure 2.9. Nesochthamalus intertextus (Darwin, 1854) (MZB Cru Cir 070-5). (a) Upper view, (b) side
view, (c) external view of scutum and tergum, (d) internal view of scutum and tergum, (e) mandible,
(f) mandibular palp, (g) labrum, (h) penis. Scale bars: a, ) 3 mm, c-d) 0.75 mm, e-g) 0.25 mm, h) 1 mm.
FAMILY CHTHAMALIDAE Darwin, 1854
SUBFAMILY EURAPHIINAE Newman & Ross, 1976
Genus Euraphia Conrad, 1837
Euraphia hembeli Conrad, 1837
Figure 2.10a-c, Table 2.1: species no. 53
Chthamalus hembeli Darwin, 1854: 465, fig. 5a-5d; Pilsbry 1916: 324.
Euraphia hembeli Conrad, 1837: 261, pl.20 fig.6; Newman and Ross 1976: 41; Foster and Newman
1987: 330; Southward et al. 1998: 120, fig. 1E; Paulay and Ross 2003: 307; Jones 2012: 372; Pochai
et al. 2017: 17.
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37
Material examined:
Ambon Island: 1 specimen, MZB Cru Cir 049, Asilulu, 3°40’50.4”S 127°55’27.6”E, coll. Adin, 20
Sep 2017.
Diagnosis:
Shell with interlocking teeth between plates; base with a true calcareous and complete secondary
calcification; scutum higher than wide and interlocked but not concrescent with tergum.
Description:
Shell with six plates, parietes symmetrical, calcareous, solid, separable, due to coarsely serrate
sutures with interlocking toothed structure (Fig. 2.10a-c); colour yellowish or brownish grey, inner
surface of parietes smooth, white with dark brown and pale violet horizontal striations around
aperture; external surface of shell irregularly ribbed around basal margin, basis calcareous; orifice
rhomboidal; tergum and scutum separable; scutum triangular, occluding margin with strong teeth;
tergum strongly marked with 10-12 lateral depressor crests, scutal margin strongly articulated.
Measurements for one specimen are presented in Supplementary Table 7.9.
Distribution:
Euraphia hembeli has been recorded from the Mediterranean, West Africa, Indian Ocean:
Ceylon; Andaman Sea, Cocos-Keeling Islands; Malay Archipelago (Sunda Islands); Pacific Ocean
(Japan; Caroline Islands; Hawaiian Islands, California (Newman and Ross 1976; Jones 2012;
Barrett and Freeman 2016; Pochai et al. 2017). In this study, Euraphia hembeli was found
on Ambon Island at Asilulu on rocks (a map with the occurrence of Euraphia hembeli in the
Moluccas is shown in Supplementary Fig. 7.2).
Remarks:
Euraphia hembeli has a true calcareous basis and complete secondary calcification on its
parietal wall and basis (Southward et al. 1998). It can be also distinguished from other species
of the genus Euraphia by its size (up to 30 mm) and the presence of strong marked lateral
depressor crests (between 10-12 in number) (Pochai et al. 2017).
Figure 2.10. Euraphia hembeli Conrad, 1837 (MZB Cru Cir 049). (a) Upper view of E. hembeli which is
overgrown with other species of barnacles in its operculum, (b) right side view, (c) left side view. Scale
bar: 16 mm.
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Chapter 2
Genus Microeuraphia Poltarukha, 1997
Microeuraphia sp.
Figure 2.11a-o, Table 2.1: species no. 55
Material examined:
Seram Island: 2 specimens, MZB Cru Cir 138, Pantai Waimeteng-Piru, 3°04’15.3”S 128°11’45.8”E,
coll. P. Pitriana & D. Tala, 21 Sep 2017.
GenBank accession numbers: COI gene (MK995389, MK995390), 18S (MK981401, MK981402).
Figure 2.11. Microeuraphia sp. (MZB Cru Cir 136-1). (a) Upper view, (b) lower view, (c) side view, (d)
external view of scutum and tergum, (e) internal view of scutum and tergum, (f) cirrus I, (g) cirrus II,
(h) cirrus III, (i) cirrus IV, (j) cirrus V, (k) cirrus VI, (l) penis, (m) maxilla, (n) maxillule, (o) mandible. Scale
bars: a-c) 3 mm, d-l) 0.5 mm, m-o) 0.25 mm.
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39
Diagnosis:
Shell small with six thin plates; basis membranous; scutum and tergum remain articulated,
scutum higher than wide; mandible tridentate; caudal appendage absent; one individual with
two penises.
Description:
Shell brownish (Fig. 2.11a, b), depressed (Fig. 2.11c); orifice diamond shaped (Fig. 2.11a, b);
overlap of ‘rostrolateral’ forming T junction (Fig. 2.11b); scutum and tergum triangular, tergal
margins straight (Fig 2.11d, e); cirrus I with anterior ramus longer than posterior (Fig 2.11f);
mandible with smooth tridentate teeth (2.11o). Ranges of basal length 3.6-9.9 mm, basal
width 3.0-9.1 mm, height 1.2-2.2 mm. Orifice of diamond shape with orifice length 1.5-4.5 mm,
orifice width 0.7-3.6 mm (measurements for two specimens are presented in Supplementary
Table 7.10).
Distribution:
In this study, Microeuraphia sp. was found on Seram Island (at Pantai Waimeteng, Piru) (a map
with the occurrence of Microeuraphia sp. in the Moluccas is shown in Supplementary Fig. 7.5).
Remarks:
Microeuraphia sp. clustered as a unit, forming a well-supported clade in the COI tree (Fig.
2.29). Morphologically, one individual of this species exhibited two penises.
Family Chthamalidae Darwin, 1854
Subfamily Chthamalinae Darwin, 1854
Genus Chthamalus Ranzani, 1817
Chthamalus moro Pilsbry, 1916
Figure 2.12a-b, Table 2.1: species no. 57
Chthamalus malayensis: Utinomi 1954: 18–21 (part.); Karande & Palekar 1963 (part.); Pope 1965
(part.); Newman & Ross 1976 (part.).
Chthamalus moro Pilsbry, 1916: 311; Nilsson-Cantell 1921: 277; Broch 1922: 307 (part.); Hiro 1937b:
49; Rosell 1972: 178; Dong et al. 1980: 125; Ren 1984: 153; Southward & Newman 2003: 798, fig.
2B; Chan et al. 2009a: 165, fig.141.
non Chthamalus moro Broch, 1922: 307 (part.); Broch 1931: 56 (includes a euraphiid).
non Chthamalus moro Nilsson-Cantell, 1934: 50 (a euraphiid).
non Chthamalus moro Poltarukha, 2001b: 160 (= C. malayensis).
Material examined:
Ambon Island: 2 specimens, MZB Cru Cir 036, Alang, 3°45’11.0”S 128°01’23.1”E, coll.
Adin, 20 Sep 2017; 2 specimens, MZB Cru Cir 037, Asilulu, 3°40’50.4”S 127°55’27.6”E,
coll. Adin, 20 Sep 2017; 10 specimens, MZB Cru Cir 038, Hila, 3°34’57.5”S 128°05’31.9”E,
coll. Adin, 20 Sep 2017; 7 specimens, MZB Cru Cir 039, Hatu, 3°43’52.7”S 128°02’51.4”E,
40
-
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Chapter 2
coll. Adin, 20 Sep 2017; 44 specimens, MZB Cru Cir 040, Mamala, 3°33›20.5»S
128°11›32.8»E, coll. Adin, 20 Sep 2017; 38 specimens, MZB Cru Cir 041, Morella,
3°31’06.5”S 128°13’18.0”E, coll. Adin, 20 Sep 2017; 25 specimens, MZB Cru Cir 042,
Wakasihu, 3°46’27.6”S 127°56’36.6”E, coll. Adin, 20 Sep 2017.
Pombo Island: 4 specimens, MZB Cru Cir 043, Pombo, 3°31’55.5”S 128°22’28.8”E, coll. P.
Pitriana & D. Tala, 8 Sep 2016.
Saparua Island: 32 specimens, MZB Cru Cir 044, Dermaga Ihamahu, 3°31’13.0”S
128°41’14.9”E, coll. P. Pitriana & D. Tala, 11 Apr 2016; 31 specimens, MZB Cru Cir 045,
Kulur, 3°29’48.5”S 128°36’10.7”E, coll. P. Pitriana & D. Tala, 20 Sep 2016; 40 specimens,
MZB Cru Cir 046, Waisisil, 3°34’48.6”S 128°39’04.8”E, coll. P. Pitriana & D. Tala, 8 Apr
2016.
Seram Island: 15 specimens, MZB Cru Cir 047, Desa Murnaten, 2°51’48.8”S 128°20’32.3”E,
coll. P. Pitriana & D. Tala, 19 Sep 2017.
GenBank accession numbers: COI gene (MK995377 - MK995388), 18S (MK981391 MK981400).
Diagnosis:
Shell with six plates; rostrum and carina with radii; rostral lateral lacking radii; carinal lateral
absent; base membranous; conical spines on cirrus I absent; basal guard on apex setae of
cirrus II absent.
Description:
Shell white to grey, surface with strong, radiating lines, orifice elliptical (Fig. 2.12a); parietes solid (Fig.
2.12b); scutum triangular, tergal margin straight; tergum triangular, scutal margin curved; conical spines
on dorsal side of cirrus I absent, cirrus II with multi-cuspidate setae without basal guard. Basal length
2.4-5.1 mm, basal width 1.4-4.1 mm and height 0.8-1.7 mm. Orifice length 1.0-3.4 mm and orifice
width 0.7-1.7 mm (measurements for 25 specimens are presented in Supplementary Table 7.11).
Distribution:
Chthamalus moro is widely distributed in the Indo-Pacific-Indonesia, Philippines, Taiwan, Xisha
Islands, Ryukyu Islands, Palau, Mariana Islands, Caroline Islands, Fiji, and Samoa (Southward
and Newman 2003). In this study, C. moro was found on the islands of Ambon (at Hatu,
Mamala, Alang, Asilulu, Hila, Morella, Wakasihu), Pombo, Seram (at Murnaten), and Saparua
(at Ihamahu, Kulur, and Waisisil) on mangroves, stone, port pole, mollusd shell, shells of
Tetraclita squamosa, Tesseropora rosea and Capitulum mitella (a map with the occurrence of
Chthamalus moro in the Moluccas is shown in Supplementary Fig. 7.3).
Remarks:
Species of the genus Chthamalus are very difficult to distinguish in the field. Chthamalus moro
has a stellate appearance and is smaller than C. malayensis (Southward and Newman 2003).
In addition, conical spines on the dorsal side of cirrus I are absent and setae on cirrus II are
without basal guards in C. moro.
Checklist of Cirripedia from the Indonesian Moluccas
41
Figure 2.12. Chthamalus moro Pilsbry, 1916 (MZB Cru Cir 042-1). (a) Upper view, (b) lower view. Scale
bar: 1 mm.
Superfamily Tetraclitoidea Gruvel, 1905
Family Tetraclitidae Gruvel, 1903
Subfamily Tetraclitellinae Newman & Ross, 1976
Genus Tetraclitella Hiro, 1939
Subgenus Tetraclitella Hiro, 1939
Tetraclitella divisa (Nilsson-Cantell, 1921)
Figure 2.13a-b, Table 2.1: species no. 59
Tetraclita divisa Nilsson-Cantell, 1921: 362, fig. 83, pl. 3 fig. 11.
Tetraclitella (Tetraclitella) divisa: Ross & Perreault 1999: 6.
Tetraclitella divisa: Ross 1968: 13; Dong et al. 1982: 111; Foster 1974: 45, figs 6E-F, 7Eal. 1984: 86; Paulay and Ross 2003: 308; Chan et al. 2009a: 208, fig. 178.
F; Bacon et
Material examined:
Ambon Island: 1 specimen, MZB Cru Cir 120, Laha, 3°43’22.5”S 128°05’02.5”E, coll. P. Pitriana
& D. Tala, 7 Sep 2016.
Diagnosis:
Shell with four plates, flattened, not strongly articulated; radii tubiferous; summit of radii
horizontal; tergal spur well separated from scutal margin.
Description:
Shell depressed, covered by furry chitinous integument; shell plates with prominent radiating
ribs; radii wide, porose, tubes running parallel to base of shell; colour of shell pale purplish;
orifice diamond shaped (Fig. 2.13a–b); scutum triangular, tergal margin straight; tergum higher
42
Chapter 2
than wide, scutal margin straight, spur short; mandible with four teeth, second and third teeth
bidentate; labrum with smooth cutting edge (measurements for one specimen are presented
in Supplementary Table 7.12).
Distribution:
Tetraclitella divisa was previously recorded from Western Africa, Java, Malaysia, Sumatra,
Northern Australia, Singapore, South China Sea, China, Taiwan, Japan, the Pacific Ocean to
Hawaii and Pitcairn (Jones and Hosie 2016). In this study, T. divisa was found on Ambon Island
at Laha on a concrete wall at the port (a map with the occurrence of Tetraclitella divisa in the
Moluccas is shown in Supplementary Fig. 7.2).
Remarks:
Tetraclita divisa exhibits a brooded phase to the cypris larval stage in the mantle cavity, whereas
most other species release the first stage nauplius (Nilsson-Cantell 1921; Hiro 1939).
Figure 2.13. Tetraclitella divisa (Nilsson-Cantell, 1921) (MZB Cru Cir 120098). (a) Upper view, (b) side
view. Scale bar: 4 mm.
Tetraclitella karandei Ross, 1971
Figure 2.14a-g, Table 2.1: species no. 60
Tetraclitella (Tetraclitella) karandei: Ross & Perreault 1999: 6.
Tetraclitella karandei Ross, 1971: 217, figs 2-3, 4A-J; Newmann & Ross 1979: 47; Chan et al. 2009a: 214,
fig.184.
Material examined:
Ambon Island: 10 specimens, MZB Cru Cir 121, Waitatiri, 3°37’04.0”S 128°16’20.3”E, coll.
P. Pitriana & D. Tala, 21 Sep 2017; 2 specimens, MZB Cru Cir 122, Asilulu, 3°40’50.4”S
127°55’27.6”E, coll. Adin, 20 Sep 2017.
Diagnosis:
Shell with four plates, tubiferous, not strongly articulated; radii tubiferous; summit of radii
horizontal and elevated above the surface of the parietes; parietes with longitudinal ribs;
scutum with nodose ornamentation.
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Description:
Shell with orifice diamond shaped, colour greyish (Fig. 2.14a); surface of parietes with chitinous
coating and fine hairs, parietes with longitudinal ribs intercalated with lower secondary and
tertiary ribs; radii broad, horizontally ridged from base to apex; scutum triangular, occluding
and basal margins almost perpendicular, tergal margin straight, surface ornamentation nodose;
tergum higher than wide, scutal margin straight, spur small; mandible with four teeth (Fig.
2.14g), labrum slightly notched, two small teeth on each cutting edge. Basal length 10.1-17.4
mm, basal width 8.2-18.2 mm, height 0.4-0.7 mm. Orifice length 3.8-5.6 mm, orifice width
2.7-5.2 mm (measurements for three specimens are presented in Supplementary Table 7.13).
Distribution:
Tetraclitella karandei was previously recorded from India, Taiwan, the Philippine (Chan et
al. 2009a). In this study, T. karandei was found on Ambon Island at Waitairi and Asilulu on
stone, on the shells of Capitulum mitella and Euraphia hembeli (a map with the occurrence of
Tetraclitella karandei in the Moluccas is shown in Supplementary Fig. 7.2).
Remarks:
Tetraclitella karandei can be distinguished by its radii, which are broad and have extended out
and over the adjoining plates. The scutum is also unique because it has nodose ornamentation
(Ross 1971).
Figure 2.14. Tetraclitella karandei Ross, 1971 (MZB Cru Cir 122-2) on tergum of Capitulum mitella. (a)
Upper view of Tetraclitella karandei on Capitulum mitella, (b) external view of scutum, (c) internal view
of scutum, (d) external view of tergum, (e) internal view of tergum, (f) maxillule, (g) mandible. Scale
bars: a) 15 mm, b-e) 1 mm, f-g) 0.25 mm.
44
Chapter 2
Subfamily Tetraclitinae Gruvel, 1903
Genus Tesseropora Pilsbry, 1916
Tesseropora rosea (Krauss, 1848)
Figure 2.15a-e, Table 2.1: species no. 61
Conia rosea Krauss, 1848: 136.
Tetraclita rosea Darwin, 1854: 335, pl.10 fig. 3a-3d; Pilsbry 1916: 260, pl. 58 fig. 4.
Tesseropora rosea Newman & Ross, 1976: 47; Anderson & Anderson 1985: 89, figs 1-10; Jones &
Anderson 1990: 13.
Material examined:
Ambon Island: 6 specimens, MZB Cru Cir 075, Rutong, 3°42›23.7»S 128°16›08.9»E, coll. P.
Pitriana, 14 Jan 2016; 1 specimen, MZB Cru Cir 076, Leahari, 3°42’45.3”S 128°16’16.5”E,
coll. P. Pitriana, 14 Jan 2016; 25 specimens, MZB Cru Cir 077, Liang, 3°30’13.3”S
128°20’34.1”E, coll. P. Pitriana & D. Tala, 7 Sept 2016.
Saparua Island: 25 specimens, MZB Cru Cir 078, Dermaga Ihamahu, 3°31’13.0”S 128°41’14.9”E,
coll. P. Pitriana & D. Tala, 11 Apr 2016; 4 specimens, MZB Cru Cir 079, Kulur, 3°29’48.5”S
128°36’10.7”E, coll. P. Pitriana & D. Tala, 20 Sep 2016; 10 specimens, MZB Cru Cir 080, Porto,
3°34›58.2»S 128°36›58.2»E, coll. P. Pitriana & D. Tala, 20 Sep 2016.
GenBank accession number: COI gene (MK995370).
Diagnosis:
Shell with four plates; wall of the parietes with a single row of parietal pore; orifice with traces
of pink in colour; oral cone relatively broad; mouthparts relatively large.
Description:
Shell steeply conical, whitish tinged pink, with longitudinal purple pinkish striations (Fig. 2.15a); four
parietal plates with single row of large, square tubes, often eroded in upper areas giving pillared
appearance (Fig. 2.15b, c); radii solid, well developed; orifice pentagonal in uneroded specimens,
triangular in eroded specimens; basis mostly calcareous; scutum thick, articular furrow short, deep,
articular ridge long, adductor ridge prominent, crests for lateral depressor faint; tergum with short,
broad spur set close to basiscutal angle, wide articular furrow, carinal depressor crests prominent
(Fig.2.15d, e); maxillule with two large setae at the lateral angle; mandible with four teeth, labrum
shallowly concave in shape, teeth on each side. Basal length 9.7-25.6 mm, basal width 9.7-24.5
mm, height 4.4-13.0 mm. Orifice length 2.9-7.8 mm, orifice width 2.3-6.9 mm (measurements for
15 specimens are presented in Supplementary Table 7.14).
Distribution:
Tesseropora rosea was originally described from a specimen collected at Algoa Bay, South
Africa (Krauss 1848; Darwin 1854) and has since been recorded from Australia (SW and SE);
Lord Howe Island and the Kermadec Islands (Jones 1990). In this study, T. rosea was found
on Ambon Island (at Rutong, Leahari, and Liang) and Saparua Island (at Ihamahu, Kulur, and
Porto) on stone and mollusc shells (a map with the occurrence of Tesseroppora rosea in the
Moluccas is shown in Supplementary Fig. 7.4).
Checklist of Cirripedia from the Indonesian Moluccas
45
Remarks:
According to Anderson and Anderson (1985), T. rosea feeds in different ways, extending the
cirral fan only in response to the fast water currents. Thus, T. rosea cannot survive in areas with
a low current velocity. Tesseropora rosea exhibits a wide distribution although the species is
represented by relatively few specimens.
Figure 2.15. Tesseropora rosea (Krauss, 1848) (MZB Cru Cir 077-1). (a) Upper view, (b) lower view, (c)
side view, (d) external view of scutum and tergum, (e) internal view of scutum and tergum. Scale bars:
a-c) 6 mm, d, e) 2 mm.
Genus Tetraclita Schumacher, 1817
Tetraclita kuroshioensis Chan, Tsang & Chu, 2007
Figure 2.16a-g, Table 2.1: species no. 62
Tetraclita squamosa viridis: Hiro 1936b: 635.
Tetraclita squamosa squamosa: Utinomi 1968a: 178.
Tetraclita pacifica Chan et al., 2007a: 88, figs 4-6.
Tetraclita kuroshioensis Chan et al., 2007: 56; Chan et al. 2009a: 192, fig. 164; Pochai et al. 2017: 21,
fig. 6.
Material examined:
Ambon Island: 1 specimen, MZB Cru Cir 097, Hatu, 3°43’52.7”S 128°02’51.4”E, coll. Adin,
20 Sep 2017; 6 specimens, MZB Cru Cir 098, Ureng, 3°40’14.0”S 127°56’47.6”E, coll.
Adin, 20 Sep 2017.
46
-
Chapter 2
Saparua Island: 1 specimen, MZB Cru Cir 100, Dermaga Ihamahu, 3°31’13.0”S
128°41’14.9”E, coll. P. Pitriana & D. Tala, 11 Apr 2016.
GenBank accession numbers: COI gene (MK995363, MK995364, MK995367), 18S
(MK981375, MK9876, MK981379).
Diagnosis:
Shell conical with four plates, tubiferous; radii solid; tergum broad, apex not beaked.
Description:
Shell with four inseparable, multi-tubiferous plates, greyish black to purplish-grey or deep
green to green, surfaces with mosaic scales pattern radiating randomly from base to apex,
internal surface of parietes smooth, white with dark grey striations around aperture; radii solid
(Fig. 2.16a-c); basis membranous; scutum larger than tergum, triangular, external surface with
horizontal striations, occluding margin with fine teeth; tergum broad, higher than wide, apex
not produced as beak, spur sharp, basi-scutal angle smaller than that of Tetraclita squamosa
(Fig. 2.16d, e); external surface of operculum grey and yellowish-light brown, internal surface
greyish-dusky green; mandible with four large teeth; maxillule not notched with eleven setae;
labrum with five small teeth on each side; cirrus I possessing serrulate setae. Basal length
12.1-21.6 mm, basal width 18.1-21.8 mm, height 7.3-10.4 mm. Orifice length 3.2-5.3 mm,
orifice width 2.4-4.2 mm (measurements for five specimens are presented in Supplementary
Table 7.15).
Figure 2.16. Tetraclita kuroshioensis Chan, Tsang & Chu, 2007 (MZB Cru Cir 097). (a) Upper view, (b)
lower view, (c) side view, (d) external view of scutum and tergum, (e) internal view of scutum and
tergum, (f) cirrus I, (g) cirrus VI. Scale bars: a-c) 7 mm, d-e) 2 mm, f, g) 1 mm.
Checklist of Cirripedia from the Indonesian Moluccas
47
Distribution:
Tetraclita kuroshioensis was previously recorded from Japan, Taiwan, Palau, and Thailand
(Chan et al. 2009a; Pochai et al. 2017). In this study, T. kuroshioensis was found on Ambon
Island (at Hatu and Ureng) and Saparua Island (at Dermaga Ihamahu) on rocks and concrete
wall of a port (a map with the occurrence of Tetraclita kuroshioensis in the Moluccas is shown
in Supplementary Fig. 7.4).
Remarks:
Tetraclita kuroshioensis and T. squamosa share great morphological similarity. However, DNA
sequences separate the two species (Chan et al. 2007), which was confirmed in this study (Fig.
2.29). Morphologically, the shape of the tergum is definitive; that of T. kuroshioensis is broader
and the apex blunter compared to T. squamosa (Chan et al. 2007).
Tetraclita squamosa (Bruguiére, 1789)
Figure 2.17 a-k, Table 2.1: species no. 63
Balanus squamosus Bruguière, 1789: 170, pl. 165 figs 9, 10.
Lepas fungites Spengler, 1790: 189.
Lepas porosa Gmelin, 1791: 3212; Wood 1815: pl. 9 fig. 4.
Tetraclita squamulosa Schumacher, 1817: 91.
Asemus porosus: Ranzani 1820: pl. 3 figs 32–35.
Conia porosa: Sowerby 1823: pl. 1.
Tetraclita porosa var. (3) viridis Darwin, 1854a: 329.
Tetraclita porosa viridis: Nilsson-Cantell 1921: 364.
Tetraclita squamosa squamosa: Pilsbry 1916: 251; Dong et al. 1982: 110, fig.
Tetraclita squamosa forma viridis: Broch 1922: 337.
Tetraclita squamosa viridis: Hiro 1936b: 635.
Tetraclita porosa perfecta Nilsson-Cantell, 1931a: 133, pl. II fig. 8a-e.
Tetraclita squamosa: Stebbing 1910: 570; Ren & Liu 1979: 339, pl. 1 figs 1–11; Yamaguchi 1987: 344;
Zevina et al. 1992: 45, fig. 30; Chan 2001: 625, fig. 8; Chan et al. 2007a: 82, fig. 4; Chan et al. 2009a:
195, fig. 167; Pochai et al. 2017: 25, fig.8.
Material examined:
Ambon Island: 17 specimens, MZB Cru Cir 081, Alang, 3°45’11.0”S 128°01’23.1”E,
coll. Adin, 20 Sep 2017; 15 specimens, MZB Cru Cir 082, Dermaga Liang, 3°30’13.3”S
128°20’34.1”E, coll. P. Pitriana & D. Tala, 30 Aug 2016; 2 specimens, MZB Cru Cir 083,
Dermaga Tulehu, 3°35’21.8”S 128°20’02.8”E, coll. P. Pitriana & D. Tala, 7 Sep 2016; 15
specimens, MZB Cru Cir 084, Doc Tawiri, 3°42’10.1”S 128°06’13.4”E, coll. P. Pitriana
& D. Tala, 29 Mar 2016; 2 specimens, MZB Cru Cir 085, Gudang Arang, 3°42’07.2”S
128°09’43.7”E, coll. P. Pitriana & D. Tala, 5 Sep 2016; 5 specimens, MZB Cru Cir 086,
Hila, 3°34’57.5”S 128°05’31.9”E, coll. Adin, 20 Sep 2017; 17 specimens, MZB Cru Cir
087, Hutumuri, 3°41’47.6”S 128°17’44.1”E, coll. P. Pitriana, 14 Jan 2016; 5 specimens,
48
Chapter 2
MZB Cru Cir 088, Leahari, 3°42’45.3”S 128°16’16.5”E, coll. P. Pitriana, 14 Jan 2016; 6
specimens, MZB Cru Cir 089, Tawiri, 3°42’10.1”S 128°06’13.4”E, coll. P. Pitriana & D.
Tala, 5 Sep 2016; 3 specimens, MZB Cru Cir 090, Tulehu, 3°35’21.8”S 128°20’02.8”E,
coll. P. Pitriana & D. Tala, 7 Sep 2016; 7 specimens, MZB Cru Cir 091, Waai, 3°33’23.5”S
128°19’33.9”E, coll. P. Pitriana & D. Tala, 7 Sep 2016; 2 specimens, MZB Cru Cir 092, Waai,
3°33’23.5”S 128°19’33.9”E, coll. P. Pitriana & D. Tala, 31 Mar 2017; 45 specimens, MZB
Cru Cir 096, Asilulu, 3°40’50.4”S 127°55’27.6”E, coll. Adin, 20 Sep 2017; 40 specimens,
-
MZB Cru Cir 099, Wakasihu, 3°46’27.6”S 127°56’36.6”E, coll. Adin, 20 Sep 2017.
Saparua Island: 5 specimens, MZB Cru Cir 093, Benteng Durstede, 3°34’32.8”S 128°39’34.7”E,
coll. P. Pitriana & D. Tala, 8 Apr 2016; 6 specimens, MZB Cru Cir 094, Teluk Saparua, 3°34’25.7”S
128°39’25.8”E, coll. P. Pitriana & D. Tala, 8 Apr 2016; 5 specimens, MZB Cru Cir 095, Teluk
Saparua, 3°34’25.7”S 128°39’25.8”E, coll. P. Pitriana & D. Tala, 22 Sept 2016.
GenBank accession numbers: COI gene (MK995360 - MK995362), 18S (MK981368 MK981373).
Diagnosis:
Shell conical with four plates, tubiferous; radii solid; tergum narrow, concaved, apex beaked.
Description:
Shell consisting of four fused, inseparable plates (Fig. 2.17a), parietes with eight rows of multitubiferous parietal tubes (Fig. 2.17b), external surface with longitudinal lines from base to
apex, internal surface smooth, white with purplish grey striations close to aperture; orifice
rhomboidal; basis membranous; shell greenish with brownish grey (Fig. 2.17a), external
surface of operculum brownish grey, internal surface purplish grey; scutum triangular, larger
than tergum, external surface with horizontal striations, occluding margin with very shallow
teeth; tergum narrow, apex beaked, spur long, sharp (Fig. 2.17c, d); mandible with four large
teeth, first tooth with three small spines, lower margin pectinate (Fig. 2.17k); maxillule notched
with two large setae above notch, elevan small setae below notch (Fig. 2.17j); labrum with
four large teeth on each side of notch; cirrus I with bidentate serrulate setae (Fig. 2.17e).
Basal length 20.1-30.2 mm, basal width 19.3-28.3 mm, height 11.9-14.2 mm. Orifice length
4.4-7.9 mm, orifice width 3.4-7.2 mm (measurements for five specimens are presented in
Supplementary Table 7.16).
Distribution:
Tetraclita squamosa is widespread in the Indo-Pacific region, Australia, South China coast,
and Taiwan (Newman 1978; Jones et al. 2001; Chan et al. 2009a). In this study, T. squamosa
was found on Ambon Island (at Alang, Dermaga Liang, Dermaga Tulehu, Doc Tawiri, Gudang
Arang, Hila, Hutumuri, Leahari, Tawiri, Tulehu, Waai, Asilulu, Wakasihu) and Saparua Island (at
Benteng Duurstede and Teluk Saparua) on stone, rocks, shipyards, concrete bridges and walls
of the port (a map with the occurrence of Tetraclita squamosa in the Moluccas is shown in
Supplementary Fig. 7.4).
Checklist of Cirripedia from the Indonesian Moluccas
49
Remarks:
Tetraclita squamosa has characteristic green parietes (Yamaguchi 1987) and a wide distribution
throughout the Indo-Pacific (Newman and Ross 1976). However, the taxonomy of Tetraclita
squamosa has been confusing due to a high degree of morphological variation, and it is now
considered a species complex. Tetraclita squamosa and Tetraclita japonica can be separated
using characters such as the shape of the parietes, scutum geometry, and mandible structures
(Darwin 1854; Pilsbry 1916). In addition, a key character for T. squamosa is the tergum with a
beak on its apex (Chan et al. 2009a).
Figure 2.17. Tetraclita squamosa (Bruguiére, 1789) (MZB Cru Cir 081-3). (a) Upper view, (b) lower view,
(c) external view of scutum and tergum, (d) internal view of scutum and tergum, (e) cirrus I, (f) cirrus
II, (g) cirrus III, (h) cirrus IV, (i) cirrus VI, (k) maxillule, (l) mandible. Scale bars: a, b) 8 mm, c, d) 2 mm,
e, f) 1 mm, g-i) 2 mm, j, k) 0.5 mm.
50
Chapter 2
Subfamily Newmanellinae Ross & Perreault, 1999
Genus Yamaguchiella Ross & Perreault, 1999
Yamaguchiella coerulescens (Spengler, 1790)
Figure 2.18a-g, Table 2.1: species no. 64
Lepas coerulescens Spengler, 1790: 191.
Tetraclita coerulescens: Darwin 1854: 342, pl. 11 figs 4a-d; Hoek 1883: 161, pl. 13 fig. 34; Pilsbry 1916:
259; Nilsson-Cantell 1938: 77; Newman & Ross 1976: 47; Dong et al. 1982: 111; Zevina et al. 1992:
48, fig. 31.
Yamaguchiella (Yamaguchiella) coerulescens: Ross & Perreault 1999: 5; Jones & Hosie 2016: 271; Chan
et al. 2009a: 202, fig. 173.
Material examined:
Ambon Island: 13 specimens, MZB Cru Cir 123, Gudang Arang, 3°42’07.2”S 128°09’43.7”E,
coll. P. Pitriana & D. Tala, 5 Sep 2016; 4 specimens, MZB Cru Cir 124, Dermaga Tulehu,
3°35’05.4”S 128°19’43.3”E, coll. P. Pitriana & D. Tala, 7 Sep 2016; 5 specimens, MZB Cru
Cir 125, Tulehu, 3°35’21.8”S 128°20’02.8”E, coll. Adin, 19 Sep 2017; 14 specimens, MZB
Cru Cir 126, Doc Tawiri, 3°42’10.1”S 128°06’13.4”E, coll. P. Pitriana & D. Tala, 29 Mar
2016; 8 specimens, MZB Cru Cir 127, Tawiri, 3°42’10.1”S 128°06’13.4”E, coll. P. Pitriana
& D. Tala, 5 Sep 2016; 2 specimens, MZB Cru Cir 128, Galala, 3°41’22.2”S 128°10’52.6”E,
coll. P. Pitriana & D. Tala, 6 Sep 2016; 11 specimens, MZB Cru Cir 129, Waai, 3°33’23.5”S
128°19’33.9”E, coll. P. Pitriana & D. Tala, 7 Sep 2016; 4 specimens, MZB Cru Cir 130,
Pelabuhan Yos Sudarso, 3°41’36.5”S 128°10’35.6”E, coll. P. Pitriana & D. Tala, 6 Sep 2016.
Saparua Island: 1 specimen, MZB Cru Cir 131, Kulur, 3°29’48.5”S 128°36’10.7”E, coll. P.
Pitriana & D. Tala, 20 Sep 2016.
GenBank accession number: 18S (MK981381).
Diagnosis:
Shell with the upper part tinged greenish-blue, longitudinally ribbed; radii moderately wide,
with their summits oblique; scutum with a small adductor and extremely prominent articular
ridge, united together and forms a small sub-cylindrical cavity; tergum with the spur not joined
to the basi-scutal angle.
Description:
Shell low conical to cylindro-conic (Fig. 2.18c) with four multi-tubiferous plates (Fig. 2.18b),
parieties greenish or greyish with radiating lines (Fig. 2.18a); radii wide, summits oblique; basis
calcareous, radii wide, tubiferous, summits oblique; orifice occluded wholly by scutum (Fig.
2.18a); scutum and tergum strongly articulated (Figs 2.18d, e); mandible with four teeth, the
third teeth is tridentate (Fig. 2.18g); labrum with three large teeth on each side of cutting
edge; penis with basidorsal point. Basal length 8.3-29.0 mm, basal width 8.5-27.8 mm, height
5.7-17.7 mm. Orifice length 4.2-11.5 mm, orifice width 3.2-11.8 mm (measurements for 25
specimens are presented in Supplementary Table 7.17).
Checklist of Cirripedia from the Indonesian Moluccas
51
Distribution:
Yamaguchiella coerulescens was previously recorded from the Indo-west Pacific: the Indian
Ocean, Bay of Bengal, Mergui Archipelago, Kei Islands, Banda Island, Malay Archipelago, Sulu
Archipelago, Vietnam, China, Philippines, Goram Island, Palao Island, and Taiwan (Jones and
Hosie 2016). In this study, Y. coerulescens was found on Ambon Island (at Tulehu, Dermaga
Gudang Arang, Waai, Doc. Tawiri, Liang, Galala, Dermaga Yos Sudarso) and Saparua Island (at
Kulur) on stone (a map with the occurrence of Yamaguchiella coerulescens in the Moluccas is
shown in Supplementary Fig. 7.6).
Remarks:
The subgenus Yamaguchiella was proposed by Ross and Perreault (1999) in honour of Toshiyuki
Yamaguchi (Chiba University Japan), in appreciation of his contributions to the knowledge of
recent and fossil barnacles.
Figure 2.18. Yamaguchiella coerulescens (Spengler, 1790) (MZB Cru Cir 123-2). (a) Top view, (b) bottom
view, (c) side view, (d) external view of scutum and tergum, (e) internal view of scutum and tergum, (f)
maxillule, (g) mandible. Scale bars: a-c) 7 mm. d, e) 1 mm, f, g) 0.5 mm.
Genus Yamaguchiella Ross & Perreault, 1999
Subgenus Neonrosella Jones, 2010
Neonrosella vitiata (Darwin, 1854)
Figure 2.19a-f, Table 2.1: species no. 65
52
Chapter 2
Tetraclita vitiata Darwin, 1854: 340, pl. 11 fig. 3a-e.
Tetraclita (Tetraclita) vitiata: Rosell 1972: 214.
Newmanella vitiata: Ikeya & Yamaguchi 1993: 93; Jones et al. 1990: 14.
Yamaguchiella (Rosella) vitiata: Ross & Perreault 1999: 5.
Yamaguchiella (Neonrosella) vitiata: Jones 2010: 214.
Neonrosella vitiata: Sukparangsi et al. 2019:4, figs 1-4.
Material examined:
Ambon Island: 3 specimens, MZB Cru Cir 132, Liang, 3°30’13.3”S 128°20’34.1”E, coll. P.
Pitriana & D. Tala, 7 Sep 2016.
Banda Neira Island: 1 specimen, MZB Cru Cir 133, Banda Neira, 4°31’22.8”S 129°53’52.5”E,
coll. P. Pitriana, 25 May 2016.
Saparua Island: 4 specimens, MZB Cru Cir 134, Tuhaha, 3°32’38.1”S 128°40’58.0”E, coll.
P. Pitriana & D. Tala, 21 Sep 2016.
GenBank accession number: 18S (MK981384).
Diagnosis:
Parietes low with wall spreading; peritreme slightly toothed; base calcareous with two rows
of irregular shape and size of parietal tubes; tergum with broad spur; lateral scutal depressor
crests numerous and deep; five toothed mandibles; segments of posterior cirri with four pairs
spines.
Description:
Shell four plated, conical, whitish with spots of purple in upper part (Fig. 2.19a); parietal tubes
irregular shape, size unequal (Fig. 2.19b); radii moderately wide, summits oblique, interior of
irregularly branching ridges with solid interspaces (Fig. 2.19c); orifice trigonal; scutum and
tergum coalesced, strongly articulated (Fig. 2.19d, e); tergum with broad spur, lateral tergal
depressor crests on basal margin long, with numerous, deep crests, lateral scutal depressor
crests numerous, deep; mandible with five teeth (Fig. 2.19f). Basal length 15.4-22.8 mm, basal
width 13.8-22.8 mm, height 5.3-6.9 mm. Orifice length 5.2-6.9 mm, orifice width 4.6-5.4 mm
(measurements for four specimens are presented in Supplementary Table 7.18).
Distribution:
Neonrosella vitiata was previously recorded from the Indo-west Pacific, Indian Ocean, Nicobar
Island to Australia, Indonesia, Malay Archipelago, Sulu Archipelago, Philippines, and the
Pacific Ocean (Jones and Hosie 2016). Recently, Neonrosella vitiata also was discovered in
the Andaman Sea of Thailand (Sukparangsi et al. 2019). In this study, N. vitiata was found
on Ambon Island (at Liang), Banda Island and Saparua Island (at Tuhaha) on port poles, reef
and stones (a map with the occurrence of Neonrosella vitiata in the Moluccas is shown in
Supplementary Fig. 7.7).
Checklist of Cirripedia from the Indonesian Moluccas
53
Remarks:
Neonrosella vitiata can be distinguished by its irregular parietal tubes, the shape of the terga,
the five toothed mandibles and four pairs of spines on the segments of the posterior cirri
(Darwin 1854).
Figure 2.19. Neonrosella vitiata (Darwin, 1854) (MZB Cru Cir 132-3). (a) Upper view, (b) lower view,
(c) side view, (d) external view of scutum and tergum, (e) internal view of scutum and tergum, (f)
mandible. Scale bars: a-c) 8 mm, d, e) 1 mm, f) 0.5 mm.
Genus Newmanella Ross, 1969
Newmanella spinosus Chan & Cheang, 2016
Figure 2.20a-e, Table 2.1: species no. 66
Newmanella radiata: Chan et al. 2009: 199, fig. 170.
Newmanella sp. Tsang et al., 2015: 325, fig. 1A; 327 fig. 2.
Newmanella spinosus Chan & Cheang, 2016: 212, figs 9-15; Pochai et al. 2017: 20, fig. 5; Sukparangsi
et al. 2019: 10, figs 5-8.
Material examined:
Ambon Island: 5 specimens, MZB Cru Cir 072, Rutong, 3°42’23.7”S 128°16’08.9”E, coll. P.
Pitriana, 14 Jan 2016.
Diagnosis:
Shell low conical to cylindro-conical; parietes discrete; base calcareous; radii broad; scutum
with very deep depressor muscle crests; cirrus II and cirrus IV having numerous triangular
spines; fourth and fifth teeth of mandible separated; cutting edge of maxillule below notch
protruding; intromittent organ of penis lacking basi-dorsal point.
54
Chapter 2
Description:
Shell low conical, four plates externally greyish in colour, parietes with deep longitudinal,
radiating lines from base to apex, internally with multiple rows of irregular parietal tubes (Fig.
2.20a, b); radii wide with horizontal striations, summits oblique (Fig. 2.20c); scutum triangular,
external surface with horizontal striations, adductor ridge conspicuous; tergum high, narrow,
basal margin with well-developed depressor muscle crests projecting beyond border; orifice
pentagonal (Fig. 2.20d, e); basis calcareous, tubiferous, tubes in single layer; mandible with
five teeth, the first tooth is the largest and separated from the rest, while the fifth tooth is the
smallest and located at the middle of lower margin; labrum with V-shaped notch, two large
teeth on the right side, five teeth on the left side of cutting margin; penis without basidorsal
point, with few bundles of setae distally. Basal length 17.4-20.9 mm, basal width 15.9-20.5
mm, height 6.8-8.9 mm. Orifice length 5.3-7.3 mm, orifice width 5.0-6.7 mm (measurements
for five specimens are presented in Supplementary Table 7.19).
Distribution:
Newmanella spinosus was previously recorded from Japan, Taiwan, Philippines, and Thailand
(Chan and Cheang 2016; Pochai et al. 2017). In the current study, this range is extended to
Rutong (on stones and reef surface), Ambon Island (a map with the occurrence of Newmanella
spinosus in the Moluccas is shown in Supplementary Fig. 7.2).
Figure 2.20. Newmanella spinosus Chan & Cheang, 2016 (MZB Cru Cir 072-1). (a) Upper view, (b) lower
view, (c) side view, (d) external view of scutum and tergum, (e) internal view of scutum and tergum.
Scale bars: a-c) 7 mm, d, e) 2 mm.
Checklist of Cirripedia from the Indonesian Moluccas
55
Remarks:
Newmanella spinosus is morphologically close to Newmanella radiata but it can be distinguished
by the morphology of the scutum, tergum, cirrus II, mandible and maxillule. N. spinosus also
has numerous spines on its cirri, especially on cirrus II, which is different from N. radiata (Chan
and Cheang 2016).
Family Balanidae Leach, 1817
Subfamily Amphibalaninae Pitombo, 2004
Genus Amphibalanus Pitombo, 2004
Amphibalanus amphitrite (Darwin, 1854)
Figure 2.21a-h, Table 2.1: species no. 87
Balanus amphitrite Darwin, 1854: 240 (part.), pl. 5. figs 2a-d, i-k, m-o; Weltner 1897:264; Hoek 1913:
167; Pilsbry 1916: 89; Zevina et al. 1992: 89, fig. 61; Puspasari et al. 2001b: 7.
Balanus amphitrite var. (1) communis Darwin, 1854: 240, pl. 5 fig. 2e, h, l.
Balanus amphitrite communis: Nilsson-Cantell 1921: 311, fig. 64.
Balanus amphitrite forma hawaiiensis Broch, 1922: 314, fig. 56 (part.).
Balanus amphitrite forma denticulata Broch, 1927b: 133, fig. 14 (part.).
Balanus amphitrite hawaiiensis: Hiro 1937c: 432, figs 20, 21.
Balanus amphitrite cochinensis Nilsson-Cantell, 1938b: 43, fig. 11a-e.
Balanus amphitrite var. fluminensis Oliveira, 1941: 21, pl. 4 fig. 4, pl. 5 figs 1, 2, pl. 8 figs 1-5.
Balanus amphitrite var. aeratus Oliveira, 1941: 22, pl. 4 fig. 5, pl. 9 figs 1-4.
Balanus amphitrite herzi Rogers, 1949: 8, pl. 1 figs 6, 12-15.
Balanus amphitrite franciscanus Rogers, 1949: 9, pl. 1 figs 5, 7, 16-19.
Balanus amphitrite var. columnarius Tarasov & Zevina, 1957: 179, 184, fig. 68 a-e.
Balanus amphitrite denticulata Henry, 1959: 192, pl. 1 fig. 5, pl. 3 fig. 7, upper row right.
Balanus amphitrite amphitrite: Harding 1962: 274, pl. 1a-g, pl. 2a-k; Dong et al. 1982: 90, fig. A-E; Rosell
1981: 302.
Balanus amphitrite var. hawaiiensis: Stubbings 1963b: 15.
Amphibalanus amphitrite: Pitombo 2004: 263, 274, figs 2A, B, 7A, B, 8C; Chan et al. 2009a: 241; Chen
et al. 2014: 1071; Shahdadi et al. 2014: 213; Pochai et al. 2017: 27, fig. 9; Xu 2017: 48.
Material examined:
Ambon Island: 4 specimens, MZB Cru Cir 005, Galala, 3°41’22.2”S 128°10’52.6”E, coll. P.
Pitriana & D. Tala, 6 Sep 2016.
Saparua Island: 4 specimens, MZB Cru Cir 007, Desa Mahu, 3°32’19.6”S 128°41’17.3”E,
coll. P. Pitriana & D. Tala, 11 Apr 2016; 5 specimens, MZB Cru Cir 008, Negeri Mahu,
3°31’52.9”S 128°41’12.4”E, coll. P. Pitriana & D. Tala, 11 Apr 2016; 2 specimens, MZB Cru
Cir 009, Tuhaha, 3°32’38.1”S 128°40’58.0”E, coll. P. Pitriana & D. Tala, 21 Sep 2016.
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Chapter 2
Diagnosis:
Primary parietal tubes with transverse septa; exterior of shell with longitudinal purple
striations, horizontal striations absent; tergum short with wide spur; cirri III-VI with erect teeth
below posterior angles of distal; cirrus III without complex setae.
Description:
Shell six plated, conical, round;, externally smooth, white with groups of well-spaced, dark
purple vertical stripes, horizontal striations on shell surface absent (Fig. 2.21a, b), interior of
parietes with single row of tubes (Fig. 2.21c); radii solid, wide; alae with summits moderately
oblique (Fig. 2.21a, b); basis porous, calcareous; scutum externally striped, internally with
prominent articular ridge 3/5 length of tergal margin, well separated from straight adductor
ridge, occluding margin toothed, lateral depressor muscle pit small (Fig. 2.21d, e); tergum with
spur wider than long, less than its own width from basi-scutal angle (Fig. 2.21d, e); mandible
with four teeth (Fig. 2.21g); labrum multi-denticulate (Fig. 2.21h). Basal length 3.1-17.8 mm;
basal width 2.8-17.6 mm; height 2.1-10.8 mm; orifice length 1.5-8.1 mm; orifice width 1.5-5.4
mm (measurements for 15 specimens are presented in Supplementary Table 7.20).
Figure 2.21. Amphibalanus amphitrite (Darwin, 1854) (MZB Cru Cir 005-4). (a) Upper view, (b) side
view, (c) lower view, (d) external view of scutum and tergum, (e) internal view of scutum and tergum,
(f) maxilla, (g) mandible, (h) labrum. Scale bars: a-c) 4 mm, d, e) 1 mm, f-h) 0.5 mm.
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57
Distribution:
Amphibalanus amphitrite is commonly found on beaches and in estuaries, lives attached to
harsh natural substrate, such as bedrock, rocks, shells of molluscs, as well as the roots and
trunks of mangrove trees. Many specimens also stick to artificial substrates, such as ship
hulls and the walls and pillars of docks. Amphibalanus amphitrite is spread globally tropical
and subtropical waters (Zullo et al. 1972; Henry and McLaughlin 1975; Chen et al. 2014).
In this study, A. amphitrite was found on the islands of Ambon (at Galala) and Saparua (at
Desa Mahu, Negeri Mahu and Tuhaha) on stone, mollusc shells and the capitulum of Lepas
anserifera (a map with the occurrence of Amphibalanus amphitrite in the Moluccas is shown
in Supplementary Fig. 7.4).
Remarks:
Amphibalanus amphitrite is difficult to distinguish from two other members of the subgenus
Balanus, i.e., Balanus crenatus Bruguière, 1789 and Balanus trigonus Darwin, 1854. However,
A. amphitrite can usually be distinguished from the other species by the multi-denticulated
labrum and also by the colour pattern of the parietes and sheath (Henry and McLaughlin 1975).
Amphibalanus reticulatus (Utinomi, 1967)
Figure 2.22a-o, Table 2.1: species no. 88
Balanus amphitrite var. (1) communis Darwin, 1854: 240, pl. 5 fig. 2e, h, l (part.).
Balanus amphitrite forma communis: Broch 1922: 314 (part.).
Balanus amphitrite forma hawaiiensis Broch, 1922: 314 (part.).
Balanus amphitrite communis: Hiro 1938a: 301, fig. 1a, b.
Balanus amphitrite cirratus: Zevina & Tarasov 1963: 89, fig. 10a-e.
Balanus amphitrite var. variegatus: Stubbings 1963a: 329, fig. 2a-e.
Balanus amphitrite variety: Southward & Crisp 1963: 43, fig. 23.
Balanus amphitrite tesselatus Utinomi, 1964: 52, pl. 26 fig. 11.
Balanus amphitrite var. denticulata: Karande & Palekar 1966: 145, fig. 7, pl.1 fig. 7, pl. 4 row 5 (part.).
Balanus variegatus tesselatus Utinomi & Kikuchi, 1966: 5.
Balanus amphitrite amphitrite: Stubbings 1967: 271, fig. 14d-f (part.).
Balanus reticulatus: Utinomi 1967: 216, figs 9a, b, 10a, b, 11a-e, pl. 6 figs 7, 8 (part.); Dong et al. 1982:
91, fig. A-C; Zevina et al. 1992: 92, fig. 63; Puspasari et al. 2001b.
Amphibalanus reticulatus: Pitombo 2004: 274; Chan, et al. 2009a: 234, fig. 200; Pochai et al. 2017: 26,
fig. 10; Xu 2017: 43, figs 10, 39.
Material examined:
Ambon Island: 5 specimens, MZB Cru Cir 012, Yos Sudarso, 3°41’36.5”S 128°10’35.6”E,
coll. P. Pitriana & D. Tala, 6 Sep 2016.
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Chapter 2
Diagnosis:
Primary parietal tubes with transverse septa; exterior of shell with longitudinal and horizontal
striations; anterior margin of cirrus III with conical denticles, erect hooks below posterior
angles of distal articles of rami present.
Description:
Shell conic or cylindric; six parietal plates, externally smooth, white with groups of well-spaced
purple, or purple-pink vertical stripes intersecting with transverse striations (Fig. 2.22a, b);
parietes with single row of internal tubes; alae with summits moderately oblique; radii narrow,
summits oblique; orifice toothed; basis calcareous, porous; scutum triangular, externally flat,
internally adductor ridge conspicuous, short, low, well separated from prominent articular
ridge; tergum flat, spur narrow, short, basiscutal angle acute, scutal margin straight; scutum
triangular, occluding margin toothed; tergum flat, basiscutal angle acute, scutal margin straight
(Fig. 2.22c, d); cirrus II with simple spinules or conic teeth on outer face near anterior margin
(Fig. 2.22f); maxilla bilobed, dense setae on margin (Fig. 2.22k); maxillule not notched, setae
on upper and lower margins (Fig. 2.22l); mandible with four teeth (Fig. 2.22m); mandibular
palp with setulae on lower margin, pinnate setae on upper margin; labrum simple with four
teeth and setulae on crest on each side of deep notch (Fig. 2.22n). Basal length 7.7-16.3 mm;
basal width 2.9-15.5 mm; height 3.2-10.5 mm. Orifice length 3.5-8.9 mm; orifice width 2.5-7.1
mm (measurements for five specimens are presented in Supplementary Table 7.21).
Distribution:
Amphibalanus reticulatus is native to the Indo-Pacific region and has been introduced by
shipping to tropical-subtropical waters of the Eastern Pacific (Coles et al. 1999; Carlton et al.
2011). A. reticulatus can be found from Japan to the Malay Archipelago, east Asia from the
Yellow Sea to Gulf of Siam, from Malaysia to southeast Africa, in the Mediterranean Sea, West
Africa, the Southeast United States to the West Indies (Henry and McLaughlin 1975).
In this study, A. reticulatus was found on the islands of Ambon (at the port of Yos Sudarso)
on stone and concrete wall of the port (a map with the occurrence of Amphibalanus reticulatus
in the Moluccas is shown in Supplementary Fig. 7.2).
Remarks:
Amphibalanus reticulatus can be confused with A. amphitrite. However, the shell of A.
reticulatus exhibits clear vertical and horizontal striations, whilst A. amphitrite shows only
vertical purple striations on all shell plates (Henry and McLaughlin 1975).
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59
Figure 2.22. Amphibalanus reticulatus (Utinomi, 1967) (MZB Cru Cir 012-1). (a) Upper view, (b) side
view, (c) external view of scutum and tergum, (d) internal view of scutum and tergum, (e) cirrus I, (f)
cirrus II, (g) cirrus III, (h) cirrus IV, (i) cirrus V, (j) cirrus VI, (k) maxilla, (l) maxillule, (m) mandible, (n)
labrum, (o) penis. Scale bars: a, b) 4 mm, c-g) 1 mm, h-j, o) 2 mm, k-n) 0.5 mm.
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Chapter 2
Amphibalanus variegatus (Darwin, 1854)
Figure 2.23a-p, Table 2.1: species no. 89
Balanus amphitrite var. (8) variegatus Darwin, 1854: 241.
Balanus amphitrite var. stutsburi Krüger, 1914: 437.
Balanus concavus sinensis Broch, 1931: 63, fig. 23.
Balanus amphitrite rafflesia Nilsson-Cantell, 1934a: 64.
Balanus amphitrite var. cirratus: Pope 1945: 362, pl. 28 fig. 6, pl. 29 fig. 6, pl. 30 figs 13, 14.
Balanus amphitrite cirratus: Skerman 1960: 610, figs 1, 3 (non Balanus amphitrite cirratus Darwin,
1854).
Balanus variegatus: Harding 1962: 291, pl. 10 figs a-k; Zevina et al. 1992: 92, fig. 64.
Balanus variegatus var. cirratus: Pope 1966: 179.
Balanus amphitrite: Foster 1967: 83 (part.).287
Balanus kondakovi: Henry & McLaughlin 1975: 78 (part., New Zealand specimens; non B. kondakovi
Tarasov & Zevina, 1957).
Balanus variegatus variegatus: Foster 1979: 111, fig. 67, pl. 14b.
Balanus cirratus: Ren & Liu 1978: 145, figs 14, 15 (1-13), pl. 4 figs 15-20, pl. 5 figs 1-6.
non Balanus amphitrite variegatus: Nilsson-Cantell 1934a: 60.
non Balanus variegatus: Henry & McLaughlin 1975: 78, fig. 17, pls. 6, 7; Utinomi 1968b: 171 (= B.
cirratus).
Amphibalanus variegatus: Pitombo 2004: 274; Horikoshi & Okamoto 2005: 49, fig.3.
Material examined:
Ambon Island: 15 specimens, MZB Cru Cir 014, Waitatiri, 3°37’04.0”S 128°16’20.3”E,
coll. P. Pitriana & D. Tala, 19 Sep 2017.
Saparua Island: 10 specimens, MZB Cru Cir 015, Teluk Saparua, 3°34’25.7”S 128°39’25.8”E,
coll. P. Pitriana & D. Tala, 22 Sep 2016.
GenBank accession numbers: COI gene (MK995342 - MK995345), 18S (MK981355).
Diagnosis:
Primary parietal tubes with transverse septa; exterior of shell with longitudinal and horizontal
striations; anterior margin of cirrus III without conical denticles, erect hooks below posterior
angles of distal articles of rami absent.
Description:
Shell steeply conical, tubular in crowded populations; six parietal plates, smooth, thin,
brownish purple externally with longitudinal stripes crosshatched by transverse bands, single
row of internal tubes (Fig. 2.23b); carina forming a spout-like projection; radii wide, summits
oblique, pink-purple; alae with summits oblique; orifice toothed (Fig. 2.23a-c); sheath purple
with white bands, vesicular; basis calcareous, porous; scutum externally with growth lines
prominent, internally with articular ridge high, adductor ridge moderately long; tergum with
spur furrow, externally purple, margins white, spur pointed, basal margin deeply excavated on
either side of spur, depressor muscle crests prominent(Fig. 2.23d, e); cirrus III without conical
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61
Figure 2.23. Amphibalanus variegatus (Darwin, 1854) (MZB Cru Cir 014-1). (a) Upper view, (b) lower
view, (c) side view, (d) external view of scutum and tergum, (e) internal view of scutum and tergum,
(f) cirrus I, (g) cirrus II, (h) cirrus III, (i) cirrus IV, (j) cirrus V, (k) cirrus VI, (l) maxilla, (m) maxillule, (n)
mandible, (o) labrum, (p) penis. Scale bars: a-c) 3 mm, d-h) 1 mm, i-k, p) 2 mm, l-o) 0.5 mm.
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Chapter 2
denticles on anterior margin (Fig. 2.23h); maxilla without notch (Fig. 2.23l), mandible with
four teeth (Fig. 2.23n), labrum notched, denticulate (Fig. 2.23o); penis with basidorsal point,
with two apical setae (Fig. 2.23p). Basal length (8.3-11.8 mm, basal width 6.9-10.4 mm, height
4.3-8.4 mm; orifice length 4.8-11.8 mm; orifice width 3.4-5.3 mm (measurements for ten
specimens are presented in Supplementary Table 7.22).
Distribution:
Amphibalanus variegatus has been reported from the Indo-west Pacific: Bay of Bengal;
Sumatra; New Zealand, Australia; Indonesia; Singapore; Vietnam; Gulf of Siam; Hong Kong;
W Kyushu; Vladivostok; and is a common fouling species (Henry and McLaughlin 1975; Jones
and Hosie 2016). In this study, A. variegatus was found on the islands of Ambon (at Waitatiri)
and Saparua (at Teluk Saparua) on stones and a plastic bag (a map with the occurrence of
Amphibalanus variegatus in the Moluccas is shown in Supplementary Fig. 7.4).
Remarks:
Amphibalanus variegatus is a member of the Balanus amphitrite complex, whose members can
be difficult to distinguish morphologically. Amphibalanus variegatus can be differentiated by its
vesicular sheath, and from A. reticulatus by features of the tergum, armature of cirrus II and the
lack of erect teeth below the posterior distal angles of cirri III-VI (Henry and McLaughlin 1975).
Amphibalanus zhujiangensis (Ren, 1989)
Figure 2.24a-j, Table 2.1: species no. 90
Balanus zhujiangensis Ren, 1989a: 467, fig. 2 (1-14).
Amphibalanus zhujiangensis: Pitombo 2004: 274; Puspasari et al. 2002: 235, figs 1A-G, 2A-H; Liu and
Ren 2007: 501; Chan et al. 2009a: 238, fig. 204.
Material examined:
Ambon Island: 10 specimens, MZB Cru Cir 016, Galala, 3°41’22.2”S 128°10’52.6”E,
coll. P. Pitriana & D. Tala, 6 Sep 2016; 3 specimens, MZB Cru Cir 017, Laha, 3°43’22.5”S
128°05’02.5”E, coll. P. Pitriana & D. Tala, 5 Sep 2016; 6 specimens, MZB Cru Cir 018,
Talake, 3°41’59.4”S 128°10’19.2”E, coll. P. Pitriana & D. Tala, 5 Sep 2016.
Saparua Island: 10 specimens, MZB Cru Cir 019, Desa Pia, 3°30›20.4»S 128°36›55.0»E,
coll. P. Pitriana & D. Tala, 21 Sep 2016; 7 specimens, MZB Cru Cir 020, Negeri Mahu,
3°31’52.9”S 128°41’12.4”E, coll. P. Pitriana & D. Tala, 11 Apr 2016; 1 specimen, MZB Cru
Cir 021, Desa Mahu, 3°32’19.6”S 128°41’17.3”E, coll. P. Pitriana & D. Tala, 11 Apr 2016.
Seram Island: 5 specimens, MZB Cru Cir 022, Desa Kasie, 2°51’05.5”S 128°32’54.1”E,
coll. P. Pitriana & D. Tala, 20 Sep 2017; 3 specimens, MZB Cru Cir 010, Lepas Pantai Kawa,
2°57’32.5”S 128°05’33.4”E, coll. P. Pitriana & D. Tala, 19 Sep 2017.
GenBank accession numbers: COI gene (MK995334, MK995336, MK995337, MK995339),
18S (MK981347, MK981349, MK981350, MK981352).
Checklist of Cirripedia from the Indonesian Moluccas
63
Diagnosis:
Primary parietal tubes without transverse septa; exterior of shell with longitudinal striations;
scutum without adductor ridge, external surface scutum with row of pits; anterior margin
of cirri III with conical denticles, erect hooks below posterior angles of distal articles of rami
present; cirrus IV with erect hooks on posterodistal angles of articles.
Description:
Shell six-plated, conic, purplish-white with longitudinal stripes of purple, not cross-hatched by
transverse striations; parietes externally smooth, parietal tubes lacking transverse septa and
subsidiary tubes; radii wide with slightly oblique summits; orifice rhomboidal, toothed (Fig.
2.24a-c); scutum trigonal, exterior of scutum with single row of pits extending down centre of
valve, occluding margin toothed, inner surface smooth, adductor ridge lacking; tergum with
carinal margin convex, spur furrow open, basal margin straight on both sides of spur (Fig.
2.24d, e); cirrus III without complex setae; cirri III–VI with erect hooks around posterior angle;
first maxilla without notch, mandible with five teeth (Fig. 2.24h). Basal length 5.8-21.6 mm;
basal width 4.8-19.2 mm; height 2.1-16.5 mm; orifice length 3.0-10.0 mm; orifice width 2.67.6 mm (measurements for eleven specimens are presented in Supplementary Table 7.23).
Figure 2.24. Amphibalanus zhujiangensis (Ren, 1989) (MZB Cru Cir 018-5). (a) Upper view, (b) lower view,
(c) side view, (d) external view of scutum and tergum, (e) internal view of scutum and tergum, (f) cirrus I, (g)
cirrus II, (h) mandible, (i) labrum, (j) penis. Scale bars: a-c) 6 mm, d-g) 1 mm, h, i) 0.5 mm, j) 2 mm.
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Chapter 2
Distribution:
Amphibalanus zhujiangensis was first recorded from the estuary of the Zhujiang River, South
China Sea (Puspasari et al. 2002). Afterwards found on Okinawa Island, Japan and Taiwan (Chan
et al. 2009a). In this study, A. zhujiangensis was found on the islands of Ambon Island (at Galala,
Laha, and Talake), Saparua Island (at Dusun Pia, Negeri Mahu, Desa Mahu), and Seram Island
(at Desa Kasie, Lepas Pantai Kawa) on stone and capitulum of Lepas anserifera (a map with the
occurrence of Amphibalanus zhujiangensis in the Moluccas is shown in Supplementary Fig.
7.8).
Remarks:
The presence of a row of pits on the external surface of the scutum and the absence of
an adductor ridge on the scutum are diagnostic for A. zhujiangensis. The species can be
distinguished from A. variegatus by characters of the shell, cirri III and cirri IV; on A. reticulatus
by characters of the shell and first maxilla. Amphibalanus zhujiangensis is distinct from A.
thailandicus in lacking transverse septa in the longitudinal tubes and a notch on the first
maxilla (Puspasari et al. 2002).
Amphibalanus sp.
Figure 2.25a-o, Table 2.1: species no. 91
Material examined:
Ambon Island: 1 specimen, MZB Cru Cir 135, Talake, 3°41’59.4”S 128°10’19.2”E, coll.
P. Pitriana & D. Tala, 5 Sep 2016; 1 specimen, MZB Cru Cir 136, Waitatiri, 3°37’04.0”S
128°16’20.3”E, coll. Adin, 19 Sep 2017.
Seram Island: 2 specimens, MZB Cru Cir 137, Dermaga Pelita Jaya, 3°00’13.5”S
128°07’09.2”E, coll. P. Pitriana & D. Tala, 21 Sep 2017.
GenBank accession numbers: COI gene (MK995349 - MK995351, MK995353), 18S
(MK981356 - MK981358, MK981360).
Diagnosis:
Primary parietal tubes with transverse septa, exterior of shell with longitudinal striations; orifice
toothed; scutum without adductor ridge; anterior margin of cirri III with conical denticles,
erect hooks below posterior angles of distal articles of rami present; cirrus IV with erect hooks
on posterodistal angles of articles; basidorsal of penis absent.
Description:
Shell six-plated, conical, whitish with dark purple transverse stripes (Fig. 2.25a, b); orifice
slightly toothed; scutum trigonal, occluding margin toothed; tergum with closed spur furrow
(Fig. 2.25c, d); mandible with five teeth (Fig. 2.25n); maxilla bilobed with dense setae only on
lower margin (Fig. 2.25l). Basal length 7.4-12.2 mm, basal width 6.3-11.8 mm, height 5.5-9.4
mm; orifice length 3.6-8.3 mm; orifice width 2.6-5.9 mm (measurements for four specimens
are presented in Supplementary Table 7.24).
Checklist of Cirripedia from the Indonesian Moluccas
65
Figure 2.25. Amphibalanus sp. (MZB Cru Cir 135). (a) Upper view, (b) side view, (c) external view of
scutum and tergum, (d) internal view of scutum and tergum, (e) cirrus I, (f) cirrus II, (g) cirrus III, (h)
cirrus IV, (i) cirrus V, (j) cirrus VI, (k) penis, (l) maxilla, (m) maxillule, (n) mandible, (l) mandibular palp.
Scale bars: a, b) 3 mm, c-g) 1 mm, h-k) 2 mm, l-o) 0.5 mm.
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Chapter 2
Distribution:
In this study, Amphibalanus sp. was found on Ambon (at Talake and Waitatiri) and Seram islands
(at Dermaga Pelita Jaya) (a map with the occurrence of Amphibalanus sp. in the Moluccas is
shown in Supplementary Fig. 7.9).
Remarks:
In the molecular phylogeny, Amphibalanus sp. forms a well-supported clade in both, the
COI and the 18S tree (Figs 2.28-29). This species also has different maxilla than those of A.
amphitrite, A. reticulatus and A. variegatus, which have setae on its upper and lower margins.
Subfamily Megabalaninae Newman, 1979
Genus Megabalanus Hoek, 1913
Megabalanus tintinnabulum (Linnaeus, 1758)
Figure 2.26a-e, Table 2.1: species no. 96
Balani Rhumphius, 1705: 121, pl. 41 figs A, C, D.
Balanus tintinnabuliformis laevis Lang, 1772: 4.
Balanus cylindraceus unicum thalamum efformans, magnis ventricosus Gaultierus, 1742: un-numbered
page, pl. 106, fig. H.
Glands de mer de la grande espèce Dezallier d’Argenville, 1742: 364, pl. 30 fig. A; 1757: 364, pl. 26 fig. A.
Lepas Tintinnabulum Linnaeus, 1758: 668; Chemnitz 1785 (part.): pl. 97 figs 830, 831 (non figs 828,
829).
Lepas calyciformis orientalis Ellis, 1758: 845, pl. 34 figs 8, 9.
Balanus tintinnabulum: Bruguière 1789 (part.): 165; Holthuis & Heerebout 1972: 24, pl. 1.
Lepas tintinnabulum: Wood 1815: 38, pl. 6 figs 1, 2.
Lepas spinosa Wood, 1815 (part.): pl. 7 fig. 4 (large shell only; small shells = M. spinosus).
Balanus tintinnabulum var. (1) communis Darwin, 1854: 195, pl. 1 figs a, b, f supra, pl. 2 figs 1 a, 1 c–e,
1 i, 1 k.
Balanus tintinnabulum var. communis: Gruvel 1905a: 211
Balanus tintinnabulum tintinnabulum: Pilsbry 1916: 55, fig. 9, pl. 10 figs 1a-e; Dong et al. 1982: 86.
Balanus tintinnabulum antillensis Pilsbry, 1916: 63, pl. 13 figs 1-2 e.
Balanus (Megabalanus) tintinnabulum forma communis Broch, 1931: 56.
Balanus tintinnabulum var. tintinnabulum; Oliveira 1941: 11, fig. 1, pl. 2 figs 1, 2, pl. 4 fig. 1, pl. 5 fig. 3,
pl. 8 fig. 6.
Megabalanus antillensis Newman & Ross, 1976: 67.
Balanus (Megabalanus) tintinnabulum tintinnabulum: Ren & Liu 1978: 121, fig. 1, pl. 1 figs 1-5.
non Lepas tintinnabulum: Spengler 1790: 180 [ = Megabalanus occator (Darwin, 1854)]
non Lepas tintinnabulum var. a: Spengler 1790: 181 (incertae sedis).
non Lepas tintinnabulum var. b: Spengler 1790: 182 [ = Striatobalanus amaryllis (Darwin, 1854)]
non Lepas tintinnabulum: Chemnitz 1785: pl. 97, figs 828, 829 [= Austromegabalanus nigrescens
(Lamarck, 1818)].
non Balanus tintinnabulum: Chenu 1843: pl. 2 fig. 8, pl. 3 fig. 5, pl. 2 fig. 8 [ = Megabalanus ajax
(Darwin, 1854)]; pl. 3 fig. 5 [= Megabalanus tulipiformis (Darwin, 1854)].
Checklist of Cirripedia from the Indonesian Moluccas
67
non Balanus tintinnabulum var. communis: Krüger 1911a: 46, pl. 3 figs 31 a1-31 b2 [= Megabalanus
volcano Pilsbry, 1916)].
non Balanus (Megabalanus) tintinnabulum: Withers 1924: pl.6 figs 4-7 [ = Megabalanus linzei (Foster,
1979)].
non Balanus tintinnabulum antillensis Pilsbry, 1927: 38, fig. 3 a-c [ = Megabalanus stultus (Darwin,
1854)]
non Balanus tintinnabulum tintinnabulum: Linzey 1942: 279 [ = Megabalanus linzei (Foster, 1979)].
non Balanus tintinnabulum: Foster 1967: 81, fig. 2a, b [ = Megabalanus linzei (Foster, 1979)].
Megabalanus tintinnabulum: Newman & Ross 1976: 68; Henry & McLaughlin 1986: 17, figs 1e, 2a, g,
h, 3a-c, 5 a-l; Zevina et al. 1992: 99, fig. 67; Pitombo 2004: 175; Chan et.al 2009a: 259, fig. 224;
Pochai et.al 2017: 28, fig.11.
Material examined:
Ambon Island: 3 specimens, MZB Cru Cir 066, Laha, 3°43’22.5”S 128°05’02.5”E, coll. P.
Pitriana & D. Tala, 5 Sep 2016.
Saparua Island: 1 specimen, MZB Cru Cir 067, Desa Pia, 3°30’20.4”S 128°36’55.0”E, coll.
P. Pitriana & D. Tala, 21 Sep 2016.
Diagnosis:
Shell relatively large, lightly ribbed; radii wide; surface smooth without spines; tergum wider
than scutum with spur narrow and long, crests for depressor muscle weakly to moderately
well developed.
Description:
Shell cylindrical to conical, parietes purplish, smooth, with longitudinal purple striations,
tubiferous (Fig. 2.26a); radii wide, usually horizontally striated, summits horizontal, sutural
edges with regular denticles; summits of alae oblique; orifice rhomboidal, moderately small to
large, one-third to two-thirds basal diameter, subcircular to subtriangular; scutum triangular,
external surface with horizontal striations, inner surface with conspicuous articular ridge;
tergum triangular, frequently wider than scutum, external surface with horizontal striations,
spur long, narrow, external surface with median furrow, scutal margin denticulate (Fig. 2.26be); mandible with five teeth, labrum with deep cleft, three teeth on each side. Basal length
26.0-49.2 mm, basal width 29.0-43.1 mm, height 20.1-49.4 mm. Orifice length 13.7-16.3 mm,
orifice width 10.4-15.9 mm (measurements for four specimens are presented in Supplementary
Table 7.25).
Distribution:
Megabalanus tintinnabulum is a cosmopolitan species and widely distributed worldwide
(Pochai et al. 2017). In this study, M. tintinnabulum was found on Ambon Island (at Laha) and
in Saparua Island (at Desa Pia) on concrete bridge at the port, stones and reef surface (a map
with the occurrence of Megabalanus tintinnabulum in the Moluccas is shown in Supplementary
Fig. 7.4).
68
Chapter 2
Remarks:
The name Megabalanus was given by Hoek (1913), referring to the largest form of existing Balani.
With the exception of Balanus amphitrite, Darwin (1854) considered Balanus tintinnabulum
as the most difficult and variable species in the genus Balanus (Henry and McLaughlin 1986).
Megabalanus tintinnabulum can be distinguished by its large shell plates and purple surface
with irregular, unclear longitudinal stripes (Pochai et al. 2017).
Figure 2.26. Megabalanus tintinnabulum (Linnaeus, 1758) (MZB Cru Cir 066-3). (a) Upper view, (b)
external view of scutum, (c) internal view of scutum, (d) external view of tergum, (e) internal view of
tergum. Scale bars: a) 8 mm, b-e) 5 mm.
Megabalanus zebra (Darwin, 1854)
Figure 2.27a-c, Table 2.1: species no. 97
Balanus tintinnabulum var. (4) zebra Darwin, 1854: 195. pl. 1 fig. g.
Balanus tintinnabulum zebra: Pilsbry 1916: 57, pl. 10 figs 2, 3; Stubbings 1967: 264; Dong et al. 1982:
86, fig. A-C.
Balanus tintinnabulum var. zebra Karande & Palekar, 1966: 143, pl.I, fig. 2.
Megabalanus zebra: Newman & Ross 1976: 69; Henry & McLaughlin 1986: 47, figs 2f, 4j-k, 12e-l;
Pitombo 2004: 275; Chan et al. 2009a: 265, fig. 232; Pitombo et al. 2017: 135, figs 2, 4, 5, 6, 7, 8.
Material examined:
Ambon Island: 4 specimens, MZB Cru Cir 068, Galala, 3°41’22.2”S 128°10’52.6”E, coll. P. Pitriana
& D. Tala, 6 Sep 2016; 4 specimens, MZB Cru Cir 069, Laha, 3°43’22.5”S 128°05’02.5”E, coll. P.
Pitriana & D. Tala, 5 Sep 2016.
Diagnosis:
Parietes reddish purple with strong longitudinal white ribs; radii and sheath dark purple to
reddish brown; scutum with narrow tergal segment slightly inflected; tergum approximately
as wide as scutum, crest for depressor muscle prominent.
Checklist of Cirripedia from the Indonesian Moluccas
69
Description:
Shell conic, six-plated; parietes smooth, purple with well-developed white ribs and dark purple
interspaces; radii wide, summits horizontal, white with dark purple spots on proximal side (Fig.
2.27a); orifice rhomboidal; scutum triangular, external surface withe intersecting horizontal
and longitudinal striations; tergum triangular, white with purple spots in some areas, scutal
margin slightly curved, basal margin inclined, spur short (Fig. 2.27b, c ); mandible with five
teeth, labrum with deep cleft, three teeth on each side. Basal length 5.4-19.0 mm, basal
width 5.0-20.0 mm, height 4.4-13.1 mm. Orifice length 3.1-7.6 mm, orifice width 1.7-6.2 mm
(measurements for eight specimens are presented in Supplementary Table 7.26).
Distribution:
Megabalanus zebra is a well-known fouling species of ship hulls, floating structures, moveable
oil platforms, etc., and has been widely recorded from the Atlantic Ocean; W Africa; Indowest Pacific: Indian Ocean; Australia; Thailand; China; Philippines; Taiwan (Pilsbry 1896, 1916;
Stubbings 1961; Karande and Pakelar 1966; Foster and Willan 1979; Chan et al. 2009a; Jones
and Hosie 2016; Pitombo et al. 2017). In this study, Megabalanus zebra was found on Ambon
Island (at Galala and Laha) on stones and the capitulum of Lepas anserifera (a map with the
occurrence of Megabalanus zebra in the Moluccas is shown in Supplementary Fig. 7.1).
Figure 2.27. Megabalanus zebra (Darwin, 1854) (MZB Cru Cir 068-3). (a) Upper view, (b) external view
of scutum and tergum, (c) internal view of scutum and tergum. Scale bars: a, b) 4 mm, c, -d) 2 mm.
70
Chapter 2
Remarks:
Megabalanus zebra can be distinguished from other species in the Megabalanus group by, for
example, the inflection of the tergal segment of the scutum and the position of the spur (Henry and
McLaughlin 1986). M. zebra can also be differentiated by an intermediate shape of the tergum and
scutum compared to M. tintinnabulum and M. coccopoma (Pitombo et al. 2017).
2.4.2 Molecular results
In total, we produced 120 new sequences for this study (COI = 62 sequences, 18S =
58 sequences; Supplementary Table 7.1; Figs 2.28, 2.29). We downloaded a total of 172
sequences from GenBank (COI = 84 sequences, 18S = 88 sequences). The final COI alignment
used for phylogenetic analyses was 641 bp long, and included 156 sequences from 50 species
(Fig. 2.29). The 18S alignment was 1918 bp long and included 154 sequences from 83 species
(Fig. 2.28).
In general, support values (bootstrap and posterior probability) were low for both
markers, with the majority of internal nodes receiving support values below 50% bootstrap
or 0.5 posterior probability. However, there are several highly supported nodes throughout
(> 70% bootstrap; > 0.85 posterior probability), which allow us to gain insights into the
evolutionary history of the group. In general, and as expected, COI (Fig. 2.29) provided higher
resolution at terminal nodes but low resolution at deeper nodes (rapidly evolving marker),
whereas 18S (Fig. 2.28) provided higher resolution than COI at basal nodes (slowly evolving
marker). The trees resulting from the BEAST and RaxML analyses were fully congruent, with
no highly supported relationships being favoured in one analysis but not the other. The main
purpose of our phylogenetic analyses was to find out where the new accessions from the
Moluccas sequenced for this study are retrieved on the barnacle tree, and to see whether
putative species are retrieved as monophyletic. We therefore show multiple accessions per
species on the trees. The trees resulting from RaxML analyses and the concatenated analyses
are given in the Supplementary material (Supplementary Figs 7.10-13).
2. 5 Molecular study of Moluccan barnacles
The vast majority of new samples from the Moluccas produced in this study matched
sequences from the same species that are available on GenBank. For example, DNA sequence
of our Heteralepas japonica matched the sequence of H. japonica EU884146.1 and EU884169.1
from Chan, et al. (2009c); and our Nesochathamalus intertextus matched the sequence of N.
intertexus JX083869.1 from Perez-Losada, et al. (2012). This applies to all species for which we
have new sequences. The only exception is Chthamalus moro, for which one of our samples in
the 18S tree does not match the GenBank samples of that species. However, for this particular
case, the support values of that clade in the tree are very low, therefore the odd positioning is
not strongly supported (that clade is essentially a polytomy).
Checklist of Cirripedia from the Indonesian Moluccas
71
Figure 2.28. Bayesian phylogeny of 18S gene sequences. High Bayesian posterior probabilities (≥ 0.85)
are indicated by an asterisk at the respective node. Families with relevance for this study are highlighted
by coloured rectangles. Sample labels in red indicate sequences newly generated for this study. Species
names in bold indicate potential new species.
72
Chapter 2
Figure 2.29. Bayesian phylogeny of COI gene sequences. High Bayesian posterior probabilities (≥ 0.85)
are indicated by an asterisk at the respective node. Families with relevance for this study are highlighted
by coloured rectangles. Sample labels in red indicate sequences newly generated for this study. Species
names in bold indicate potential new species.
Checklist of Cirripedia from the Indonesian Moluccas
73
Two taxa for which we sequenced multiple accessions, but for which we could not assign a
species name, were retrieved in positions on the tree that lead us to propose these may constitute
new unidentified species. The first one is Amphibalanus sp., clustering as a unit in both COI and 18S
trees (Figs 2.28, 2.29; Supplementary Figs 7.10-13). The other was Microeuraphia sp., which formed
well supported and separated clades in the COI tree, and was clustered in the same unresolved
clade in 18S (Figs 2.28, 2.29; Supplementary Figs 7.10-13).
The K2P distances within Microeuraphia sp. were 1.74%±0.51% for the COI sequences.
The K2P distances between Microeuraphia sp. and other species ranged from 10.90% to
22.70%; and overall averaged distances between the species and other species were 13.82%
(Supplementary Table 7.27). Whilst for Amphibalanus sp. the K2P distances within the species
were 0.22%±0.13% for the COI sequences. The K2P distances between Amphibalanus sp. and
other species ranged from 13.34% to 18.33%; and overall averaged distances between the
species and other species were 14.37% (Supplementary Table 7.28).
2.6 Discussion
This checklist lists 97 species, including 23 new records the Moluccas and two of which
still await their species descriptions. The past record on barnacles from these islands dates
back to the Challenger (1872-1876) and Siboga (1899-1900) expeditions (Hoek 1913). Later,
Kolosváry (1950) only mentioned some balanids living in corals collected during Snellius
Expedition (1929-1930), which contrasts reports from other groups numerously collected
during the same expedition, such as in decapod crustaceans and Foraminifera.
Hoek (1913) listed a total of 210 species from the Malay Archipelago that were collected
during the Challenger and Siboga expeditions. Among these, 45 species were found in the
Moluccas. However, the majority of the Moluccan species listed by Hoek (32 species) were
deep-water barnacles found at depths of 204-2,798 m, while 10 species barnacles were
found at depths of 9-90 m, and only three inshore species were recorded: Temnaspis fissum
(Darwin, 1851) from Ternate; Yamaguchiella coerulescent (Spengler, 1790) and Tetraclitella
costata (Darwin, 1854) from Banda Island. In contrast, sampling for this study focused on
inshore habitats with only two deep-sea locations. In consequence to the different sampling
approaches, we found 24 inshore species and only one deep-sea species among the new
samples.
A comparison of the number of species previously recorded from Ambon, Seram, and
Banda by Hoek (1913) and Jones (2001, 2016) with those recorded in this study indicates that
species diversity for each island has been heavily underestimated. On Ambon, for example,
seven species were previously known compared to the 24 species listed here. For other smaller
islands such as Saparua and Pombo, no barnacle species was previously recorded. Given the
size of the Moluccan Archipelago, with ca. 1,000 islands, many of which have never been
sampled despite including relatively large islands such as Haruku, Buru, Yamdena or Wetar, a
much higher number of species can be expected in the Moluccas.
74
Chapter 2
The molecular results also indicate that the barnacle fauna of the region is understudied.
In addition to evidence for two potentially new species (see above), the generic assignment of
some described species is also challenged. For example, Amphibalanus zhujiangensis was found
to be more closely related to Megabalanus than to other Amphibalanus species, suggesting
the need to conduct in-depth research on this species to clarify its taxonomy. However, we
must caution against over interpretation of our phylogenetic trees, because the markers we
used revealed low node support overall.
The molecular phylogeny failed to reveal any biogeographic pattern of barnacles from
the Moluccas, which is not surprising given the limited scope of sampling. These points all
underline again the necessity of a more comprehensive approach to sampling in the region
as well as the need to explore more molecular markers for a truly integrative taxonomy of
barnacles, not just in the Moluccas.
2.7 Acknowledgements
The first author would like to thank Prof. Frank Riedel from the Freie Universität Berlin
for his general support. Daniel Josep Tala, Ading, Nurul Fitriya, Doni Nurdiansah, M. Masrur
Islami, and Frilla R. Saputra (all LIPI) for supporting the sampling of barnacles in Indonesia;
Bani Wahyudi and Abdul Basit for their support and comments on picture editing. Thanks to
Robert Schreiber, Philipp Vogt, Christine Zorn, Bernhard Schurian, Lukas Kirschey, Dr. Christian
Mitgutsch, and Anke Sänger (all MfN Berlin) for supporting the molecular, morphological, and
logistic work progress at the Museum für Naturkunde Berlin. We would like to thank the head
of crustacean collections at MZB (Dr. Daisy Wowor) for providing museum numbers for all
samples and her general support of our crustacean research. We would like to thank Prof.
William Anderson Newman, Prof. Benny Kwok Kan Chan, and Meng-Chen Yu for valuable
comments on the manuscript.
This study was funded by the Ministry of Research, Technology and Higher Education,
the Republic of Indonesia within the Program for Research and Innovation in Science and
Technology (RISET-Pro), World Bank Loan No. 8245-ID.
Barnacle samples were collected by the first author with permit numbers as follows:
488/IPK.3/KP/V 2016 for Banda Island; 694/IPK.3/AP/IX/2016 for the islands of Ambon and
Pombo; 737/IPK.3/AP/IX/2016 for Saparua Island; B-671/IPK.3/KP.01.06/IX72017 for South
Sulawesi; B-684/IPK.5/KP.01.06/IX/2017 for Seram Island.
75
CHAPTER 3
New insights gained from museum collections: Deep-sea
barnacles (Crustacea, Cirripedia, Thoracica) in the Muséum
National d’Histoire Naturelle, Paris, collected during the
Karubar expedition in 1991
Pipit Pitriana1, 2, 5, Diana S. Jones3, Laure Corbari4, Kristina von Rintelen1
Museum für Naturkunde – Leibniz Institute for Evolution and Biodiversity Science, Invalidenstrasse 43,
10115 Berlin, Germany
2
Research Centre for Deep-sea, Indonesian Institute of Science (LIPI), Jl. Y. Syaranamual, Poka, Tlk.
1
Ambon, Kota Ambon, Maluku, Indonesia
3
The Western Australian Museum, 49 Kew Street, Welshpool WA 6106, Locked Bag 49, Welshpool DC
WA 6986, Australia
Muséum national d’Histoire naturelle, Institut de Systématique, Évolution, Biodiversité ISYEB -
4
UMR 7205 - CNRS, MNHN, UPMC, EPHE, 57 rue Cuvier, CP 26, 75005, Paris, France
Institute of Geological Sciences, Freie Universität Berlin, Malteserstrasse 74–100 Building C and D,
12249 Berlin, Germany
5
published in: Zoosystematic and evolution, Volume 96, Issue 2, pp. 649-698
accepted: 13 August 2020, published: 28 September 2020
This is a post-peer-review, original version of an article published in a peer-reviewed,
open-access journal of Zoosystematic and evolution. The final authenticated version is
available online at: https://doi.org/10.3897/zse.96.55733
3.1 Abstract
An examination of the deep-sea barnacles (Cirripedia, Thoracica) collected by the
Karubar expedition to Indonesia (1991) and deposited in the Muséum National d’Histoire
Naturelle, Paris, identified 40 species contained in three families of stalked and five families of
acorn barnacles. Information on these species is presented, including descriptions, updated
distributions and images to aid species identification. Thirty of the species, treated herein,
are new records for the Indonesian Kei Islands and Tanimbar Island, which increases the total
number of species recorded from Kei Islands, Aru Island and Tanimbar Island to 40. This study
demonstrates the value of museum collections as a resource in biodiversity science.
76
Chapter 3
3. 2 Introduction
In 1991, scientists from France and Indonesia conducted collaborative research through
the Karubar expedition in Indonesia. The acronym for this expedition, which collected the
material reported on herein, is a contraction of the names of the Kei, Aru and Tanimbar Islands.
These Islands attracted attention after Professor Th. Mortensen’s Danish expedition to the Kei
Islands (1914–16). Mortensen suggested that the Islands were an ideal place for a marine
laboratory to study deep-sea fauna, as he had found stalked crinoids, elasipods and other
abyssal creatures at depths of 200–400 m around the Kei Islands (Crosnier et al. 1997).
The Karubar expedition was part of the MUSORSTOM-Tropical Deep-Sea Benthos
programme (1976–present). This programme was a collaboration between the Muséum
National d’Histoire Naturelle (MNHN), Paris and the Institut de Recherche pour le
Développement (IRD) (formerly ORSTOM), to explore the deep-sea fauna of the tropical IndoPacific. As the programme was inspired and guided by carcinologists, it is not surprising that ~
33% of the papers resulting from these cruises concern crustaceans, especially crabs, lobsters
and shrimps (Richer de Forges et al. 2013).
Publications on the thoracic barnacles collected by the Karubar expedition are those
of Buckeridge (1994, 1997) who focused on barnacles from the suborder Verrucomorpha.
He reported six species of verrucomorph barnacles: Newmaniverruca albatrossiana (Pilsbry,
1912), Altiverruca navicula (Hoek, 1913), Brochiverruca dens (Broch, 1932), Metaverruca recta
(Aurivillius, 1898), Rostratoverruca intexta (Pilsbry, 1912) and R. kruegeri (Broch, 1922). Here,
we present a study of the remaining barnacle material collected by the Karubar expedition, to
cover the entire taxonomic range of deep-sea barnacles collected by this expedition.
3.3 Material and Methods
In total, 459 specimens were studied at the Muséum National d’Histoire Naturelle
(MNHN), of which 89 specimens were studied in detail at the Museum für Naturkunde (MfN),
Berlin, Germany. Species were determined on external shell morphology and mouthpart and
arthropodal characters, as described in Darwin (1852, 1854), Hoek (1883, 1907, 1913), Pilsbry
(1890, 1907, 1916), Annandale (1905, 1906, 1908, 1909, 1910, 1913, 1916), Broch (1916, 1922,
1931), Foster (1974, 1978, 1980, 1981), Newman and Ross (1971, 1976), Rosell (1981, 1989,
1991), Calman (1918, 1919), Ren (1989), Chan et al. (2009, 2010) and Shalaeva and Boxshall
(2014).
Hard body parts, such as the shell (parietes) and the opercular plates, were studied using
a Leica M125 stereomicroscope. Soft body parts, such as mouthparts and cirri, were studied
using an Axioskop 20 light microscope. Mouthparts and cirri were mounted on glass slides and
examined under a light microscope. Images were taken using a stereomicroscope with a digital
camera (Leica Microsystems M205C and Leica Z16 APo-A).
Deep-sea barnacles from the Karubar expedition (1991)
77
In the “Taxonomic account” section, the diagnosis is followed by description, type
species and localities and known distributions for each species are given. Each species is listed
under its current valid binomen and all known synonyms are also provided. Photographs of
each species are provided to facilitate their recognition.
In the “Material examined” section, there are four station codes dependent on the type
of dredge used and are listed as follows: DW for dredge Warén, ED (drague épibenthique) for
epibenthic dredge, CP (chalut à perche) for beam trawl and CC (chalut à crevettes) for otter
trawl (shrimp).
Measurements were made using digital callipers (accurate to 0.1 mm) as follows: for
acorn barnacles, basal length of shell (LB), basal width (WB), orifice length (LO), orifice width
(WO) and carinal height (H); for stalked barnacles, total height (TH), capitular height (CH),
diameter of base of capitulum (DBC), distance between carina and scutum (CS), scutal length
(LS), scutal width (WS), tergal length (LT) and tergal width (WT).
3.4 Results
Morphological analyses of all the samples revealed 40 species from three families
of stalked barnacles (Heteralepadidae: two genera, three species; Poecilasmatidae: four
genera, five species; Scalpellidae: twelve genera, 21 species) and five families of acorn
barnacles (Verrucidae: two genera and species; Pachylasmatidae: one genus and species;
Archaeobalanidae: three genera, six species; Pyrgomatidae: one genus and species; Balanidae:
one genus and species).
A list of the species of Cirripedia collected during the Karubar expedition and deposited
in the Muséum National d’Histoire Naturelle (MNHN) Paris is presented, including additional
information on substrate, as well as the depth where the sample was found (Supplementary
Table 7.29).
Taxonomic account
Class Hexanauplia Oakley, Wolfe, Lindgren & Zaharoff, 2013
Subclass Thecostraca Gruvel, 1905
Infraclass Cirripedia Burmeister, 1834 (= Cirrhipèdes Lamarck, 1806)
Superorder Thoracica Darwin, 1854
Order Lepadiformes Buckeridge & Newman, 2006
Suborder Heteralepadomorpha Newman, 1987
Diagnosis:
Capitulum and peduncle without calcareous armament. According to Buhl-Mortensen and
Mifsud (2017), Heteralepadomorpha consists of seven families of poorlyknown Pedunculata.
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Chapter 3
Family Heteralepadidae Nilsson-Cantell, 1921
Heteralepadidae Nilsson-Cantell, 1921: 245
Lepadidae Darwin, 1852: 8 (part.)
Diagnosis:
Capitulum naked, thick-walled, globular, supported by stout peduncle; filamentary appendage
at base of cirrus I; caudal appendage multi-segmented; cirri ctenopod or acanthopod, rarely
lasiopod.
Type genus:
Heteralepas Pilsbry, 1907a.
Remarks:
The family consists of two genera: Heteralepas Pilsbry, 1907a and Paralepas Pilsbry, 1907a.
Genus Heteralepas Pilsbry, 1907
Alepas Darwin, 1852: 156, pl. III, figs 5, 6.
Heteralepas (Heteralepas) Pilsbry, 1907a: 100.
Heteralepas s. str. — Newman 1960: 109.
Diagnosis:
Filamentary appendage present at base of cirrus I, short; posterior rami of cirri V and VI shorter
and more slender than anterior rami.
Type species:
Alepas rex Pilsbry, 1907c: 186, fig. 3, pl. IV fig. 7; type locality: Kauai Island, Hawaiian waters.
Heteralepas japonica (Aurivillius, 1892)
Figure 3.1
Alepas japonica Aurivillius, 1892: 125. — 1894: 28, pl. II figs 14, 15, pl. VIII, figs 3, 7, pl. IX, fig. 3.
Alepas indica Gruvel, 1901: 259. — 1905a: 162, fig. 179.
Heteralepas (Heteralepas) japonica. — Pilsbry 1907a: 101.
Heteralepas (Heteralepas) japonica var. alba Krüger, 1911: 34, pl. 1, fig. 2b.
Heteralepas (Heteralepas) dubia Broch, 1922: 288, fig. 38.
Heteralepas japonica. — Pilsbry 1911: 71, fig. 4. — Zevina et al. 1992: 31, fig. 19. — Chan et al. 2009a:
61. — Chan et al. 2009b: 88–91, figs 2A–D, 3A–D, 4, 5. — Pitriana et al. 2020a: 12, fig. 3.
Material examined:
Tanimbar Island: 1 specimen, MNHN-IU-2019-4877, stn. CP 46, 08°01’S, 132°51’E, 271–273 m
depth, 29 October 1991.
Diagnosis:
Capitulum rounded, plates absent; wall of capitulum thick; orifice crenulated with no more
than two crests on carinal region; cirrus VI with caudal appendage; maxillule strongly notched.
Deep-sea barnacles from the Karubar expedition (1991)
79
Description:
Capitulum and peduncle yellowish. Orifice slightly protuberant, crenulated, occupying
one half to one third capitular length, parallel to or at oblique angle to capitulum;
integument thick, chitinous. Cirrus I with anterior rami (20-segmented) shorter than
posterior rami (25-segmented); cirrus VI with long caudal appendage, 24-segmented, 2/3
length of anterior ramus. Mandibles with four large teeth excluding inferior angle; maxillule
strongly notched, two large setae at upper angle, blade-shaped setae on cutting margin;
labrum concave with numerous teeth. Measurements of specimen: height of capitulum 18.50
mm, width 12.82 mm, thickness 5.97 mm; length of peduncle 15.26 mm, width 5.55 mm.
Distribution:
Singapore; Indo-west Pacific: Indian Ocean; Australia; Malay Archipelago; Vietnam; Condor
Island; South China Sea; East China Sea; Taiwan, Philippines; South Japan; NE New Zealand;
fouling hard rock substrata, crabs, gorgonians, antipatharians, deep-sea cables; 48–500 m depth
(Jones and Hosie 2016). Recently, the species has also been recorded from the Lifamatola Sea
and the Halmahera Sea, Indonesia (Pitriana et al. 2020a). In this study, Heteralepas japonica
was found at Tanimbar Island, Indonesia.
Type locality:
Hirado Strait, Japan, 146 m depth (Aurivillius 1892).
Figure 3.1. Heteralepas japonica (Aurivillius, 1892) (MNHN-IU-2019-4877). (a) left lateral view showing
the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c) anterior
view, orifice, (d) posterior view, capitulum and peduncle. Scale bars: a–d) 6 mm.
Genus Paralepas Pilsbry, 1907
Alepas Darwin, 1852: 156 (part.).
Heteralepas (Paralepas) Pilsbry, 1907a: 100, fig. 34A.
Paralepas s. str. — Newman 1960: 108.
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Chapter 3
Diagnosis:
Maxillule slightly notched; filamentary appendages well-developed; posterior and anterior
rami of cirri V and VI similar in length; segments of cirri V and VI square-shaped, each armed
with semi-circular brush of many setae on anterior face.
Type species:
Alepas percarinata Pilsbry, 1907c: 185, fig. 2, pl. IV, fig. 8; type locality: Molokai, Hawaiian
waters.
Paralepas minuta (Philippi, 1836)
Figure 3.2
Alepas minuta Philippi, 1836: 254, pl. 12, fig. 23.
Alepas (Paralepas) minuta. — Weltner 1897: 239.
Heteralepas (Paralepas) minuta. — Broch 1927b: 18, fig. 4, pl. 1, figs 1, 2.
Paralepas minuta nipponica Utinomi, 1970: 342.
Paralepas minuta. — Stubbings 1967: 240. — Newman 1960: 109. — Chan et al. 2009b: 66, figs 55–57.
Material examined:
Kei Islands: 6 specimens, MNHN-IU-2019-4882, Stn. CP 25, 05°30’S, 132°52’E, 336–346
m depth, 26 October 1991, attached to dead stem of gorgonian.
Tanimbar Island: 4 specimens, MNHN-IU-2019-4883, Stn. CP 85, 09°22’S, 131°14’E, 240–
245 m depth, 4 November 1991, attached to spine of sea urchin.
Diagnosis:
Capitulum yellowish with globular shape; anterior and posterior rami of cirrus V and VI similar
in length.
Description:
Capitulum yellowish, globose, small-sized, externally smooth; orifice crenulated; peduncle
rather short. Segments of rami of cirrus I wide, anterior ramus (9-segmented) shorter than
posterior ramus (13-segmented); cirrus VI with short caudal appendage, 8-segmented. Penis
annulated, setae sparsely distributed over most of surface, a few longer setae towards tip.
Maxillule notched, two large and one small setae at upper angle; mandible with four major
teeth; labrum concave, with numerous teeth. Measurements of specimen: height of capitulum
8.34 mm, width 6.67 mm, thickness 4.53 mm; length of peduncle 3.84 mm, width 2.60 mm.
Distribution:
Java Sea (Indonesia); Mediterranean Sea; West Africa; Indo-west Pacific: Indian Ocean;
Australia; Malay Archipelago; Taiwan; Japan; Philippines; Northern New Zealand; 485–736 m
depth; attached to spines of cidarids (Jones and Hosie 2016). In this study, Paralepas minuta
was found at Kei Islands and Tanimbar Island, Indonesia.
Deep-sea barnacles from the Karubar expedition (1991)
81
Type locality:
Mediterranean Sea (Chan et al. 2009b).
Remarks:
Paralepas minuta (Philippi, 1836) can be distinguished from Paralepas ovalis (Hoek, 1907)
by the presence of triangular scutal areas and from Paralepas nodulosa Broch, 1922 by the
absence of “well defined, scanty, almost thorn-like warts” (Broch 1922).
Figure 3.2. Paralepas minuta (Philippi, 1836) (MNHN-IU-2019-4882). (a) left lateral view, capitulum
and peduncle, (b) right lateral view, capitulum and peduncle, (c) anterior view, orifice. Scale bars: a–c)
5 mm.
Paralepas morula (Hoek, 1907)
Figure 3.3
Alepas morula Hoek, 1907: 35, pl. IV, figs 9–12.
Heteralepas (Paralepas) morula. — Broch 1922: 28, fig. 34.
Paralepas morula. — Newman 1960: 109.
Material examined:
Tanimbar Island: 45 specimens, MNHN-IU-2019-4878, Stn. CP 54, 08°21’S, 131°43’E,
836–869 m depth, 30 October 1991, attached to spine of sea urchin.
Diagnosis:
Capitulum globular with numerous tubercles on surface, scuta absent, orifice small, not
protuberant, peduncular length more than half capitular length.
Description:
Capitulum and peduncle yellowish. Capitulum globular with thick, chitinous, large warts
all over surface. Peduncle rather long, narrower than capitulum; surface bearing tubercles,
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smaller than those of capitulum. Cirrus I placed rather far from cirrus II; cirri II–VI similar, length
and number of segments slightly increasing from second to sixth; cirrus VI with 7-segmented
caudal appendage. Penis indistinctly segmented, few long hairs towards tip. Maxillule
notched, two large setae on upper notch; mandibles with three major teeth, inferior angle
considered as fourth tooth; labrum with continuous row of numerous short, strong, blunt
teeth. Measurements of specimen: height of capitulum 5.14 mm, width 3.69 mm, thickness
1.81 mm; length of peduncle 5.08 mm, width 1.31 mm.
Distribution:
Flores Sea (Indonesia); Philippines; Bass Strait, N of Tasmania; attached to spines of echinoids,
for example, Histocidaris elegans (Agassiz, 1879); 182–538 m depth (Jones and Hosie 2016). In
this study, Paralepas morula was found at Tanimbar Island, Indonesia.
Type locality:
Bali Sea, North of Lombok; 538 m depth (Hoek 1907).
Remarks:
Most of the smaller specimens do not show the warts that are characteristic of this species.
Figure 3.3. Paralepas morula (Hoek, 1907) (MNHN-IU-2019-4878). (a) left lateral view, capitulum and
peduncle, (b) right lateral view, capitulum and peduncle. Scale bars: a, b) 3 mm.
Suborder Lepadomorpha Pilsbry, 1916
Lepadomorpha Pilsbry, 1916: 14 (Lepadidae sensu Darwin, 1852).
Lepadoidea Darwin, 1852: 8 (nom. trans. Zevina 1978a, b).
Family Poecilasmatidae Annandale, 1910
Lepadidae Darwin, 1852: 8 (part.). — Pilsbry 1907a: 72 (part.).
Lepadinae. — Gruvel 1905: 104 (part.).
Poecilasmatinae Annandale, 1910a: 84.
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83
Poecilasmatidae. — Nilsson-Cantell 1921: 253.
Trilasmatidae Nilsson-Cantell, 1934: 40. — Hiro 1937a: 79. — Krüger 1940: 29.
Diagnosis:
Capitulum with up to five plates, including tergum and scutum; scutum in some species split
into two (resulting in seven plates); some or all plates may be degenerate or absent; umbos
of terga apical, those of carina and scuta fundamentally basal; peduncle without calcareous
scales; maxillule not stepped; cirrus I widely separated and much shorter than posterior cirri;
caudal appendages uniarticulate, spinose.
Type genus:
Poecilasma Darwin, 1852: 99.
Genus Dianajonesia Koçak & Kemal, 2008
Temnaspis Fischer, 1884: 357. — Broch 1931: 30.
Poecilasma (part). — Darwin 1852: 99. — Pilsbry 1907a: 82. — Nilsson-Cantell 1921: 253.
Dianajonesia Koçak & Kemal, 2008: 2.
Diagnosis:
Capitulum with five plates, approximate or variously reduced; scutum divided into two parts;
apex of carina extending to, or slightly overlapping basal end of terga, basally terminating in
disc.
Type species:
Poecilasma fissa Darwin, 1852: 109, pl. II, fig. 4, pl. X, fig. 29.
Dianajonesia amygdalum (Aurivillius, 1894)
Figure 3.4
Poecilasma amygdalum Aurivillius, 1894: 10, pl. 1, figs 4–6, pl. 8, fig. 4. — Nilsson-Cantell 1921: 262,
fig. 46 d–g, pl. 3, fig. 6.
Poecilasma fissum (non Darwin). — Hoek 1907: 8 (part.).
Trilasmis fissum hawaiense. — Pilsbry 1928: 306, pl. 24, figs 1–8.
Trilasmis (Temnaspis) amygdalum. — Hiro 1937a: 85, fig. 69.
Temnaspis amygdalum amygdalum. — Zevina: 1982: 70, fig. 62.
Temnaspis amygdalum. — Utinomi 1966: 5. — Dong et al. 1982: 77.
Dianajonesia amygdalum. — Koçak & Kemal, 2008: 2.
Material examined:
Tanimbar Island: 2 specimens, MNHN-IU-2019-4861, Stn. CP 79, 09°16’S, 131°22’E, 239–
250 m depth, 3 November 1991, attached to crab leg.
Diagnosis:
Capitulum broadly oval, with five smooth plates; carina not extending to area between terga;
peduncle with circles of small protuberances; cirri short.
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Chapter 3
Description:
Capitulum yellowish, with five tranparent, smooth plates. Scutum bilobed, occludent segment
narrow, bow-shaped; tergum sub-triangular, nearly half as broad as long. Carina very narrow,
slightly curved downwards, end blunt. Peduncle yellow, plainly ringed, spines absent. Cirrus I
with anterior ramus wider than posterior ramus. Maxillule notched, with two strong teeth in
upper part; mandible with four teeth, fourth pectinated, placed very close to inferior angle;
labrum with row of minute teeth. Measurements of specimen: basal diameter of capitulum
0.94 mm; capitular height 7.03 mm; total height 10.40 mm; scutal width 4.11 mm; scutal
length 5.85 mm; tergal width 1.00 mm; tergal length 3.13 mm.
Distribution:
Indo-west Pacific: Indian Ocean; Madagascar through Malaysia, Hong Kong, South China
Sea; Taiwan; Philippines; South Japan; tropical West and central Pacific Ocean to Fiji and
Hawaii; attached to decapod crustaceans; shallow water (Jones and Hosie 2016). In this study,
Dianajonesia amygdalum was found at Tanimbar Island, Indonesia.
Type locality:
Nordwachter Island, Thousand Islands, Java Sea (Aurivillius 1894).
Figure 3.4. Dianajonesia amygdalum (Aurivillius, 1894) (MNHN-IU-2019-4861). (a) left lateral view,
capitulum and peduncle, (b) right lateral view, capitulum and peduncle, (c) anterior view, orifice (d)
posterior view, carina. Scale bars: a–d) 2 mm.
Dianajonesia excavatum (Hoek, 1907)
Figure 3.5
Poecilasma excavatum Hoek, 1907: 10, pl. I, figs 5–10.
Poecilasma tridens. — Weltner 1922: 80, pl. 4, fig. 19 (non Aurivillius).
Poecilasma (Temnaspis) excavatum. — Nilsson-Cantell 1925: 16, fig. 5, pl. 1, fig. 1.
Deep-sea barnacles from the Karubar expedition (1991)
85
Trilasmis (Temnaspis) excavatum. — Hiro 1937b: 412.
Dichelaspis (Dichelaspis) tridens. — Stubbings 1936: 7, fig. 2. (non Aurivillius).
Trilasmis excavatum. — Nilsson-Cantell 1938: 9.
Temnaspis excavatum. — Broch 1931: 31, fig. 10. — Dong et al. 1982: 77.
Dianajonesia excavatum. — Koçak & Kemal, 2008: 2.
Material examined:
Tanimbar Island: 3 specimens, MNHN-IU-2019-4859, Stn. CP 78, 09°06’S, 131°24’E, 284295 m depth, 3 November 1991.
Diagnosis:
Capitulum with scutum divided into two segments parallel to occludent margin; tergum with
excavation in scutal margin near occludent margin, receiving tip of occludent segment of
scutum.
Description:
Capitulum oval, apex pointed, slightly thick, swollen. Scutum with larger segment strongly
bowed, basal margin short, apex pointed; smaller segment bowed, terminating in point at
base, tergal margin rounded, fitting exactly into excavation of tergum. Tergum triangular,
characteristic excavation at scutal margin near occludent margin. Carina narrow, terminating in
spatula-shaped disc. Cirrus I with anterior and posterior rami subequal (each five-segmented);
cirri II–VI longer, more slender; cirrus VI with caudal appendages. Penis thick, ringed, especially
mid-length, terminating in narrower, curved part. Maxillule notched, two large teeth on upper
side; mandible with four teeth, largedistance between first and second teeth. Labrum convex,
with numerous blunt teeth. Measurements of specimen: basal diameter of capitulum 1.06
mm; capitular height 7.12 mm; total height 12.69 mm; scutal width 3.08 mm; scutal length
6.09 mm; tergal width 1.30 mm; tergal length 3.47 mm.
Distribution:
Indo-west Pacific: East coast of Africa (Zanzibar); Gulf of Aden; Indonesia; Malay Archipelago;
South China Sea; East China Sea; Philippines; South Japan (Goto Island); attached to echinoid
spines, crustaceans; palinurids and cirripedes; 189–600 m depth (Jones and Hosie 2016). In
this study, Dianajonesia excavatum was found at Tanimbar Island, Indonesia.
Type locality:
Siboga station 253; 5°48.2’ S, 132°15’ E; depth: 304 m; bottom: grey clay, hard and crumbly
(Hoek 1907).
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Chapter 3
Figure 3.5. Dianajonesia excavatum (Hoek, 1907) (MNHN-IU-2019-4859). (a) left lateral view showing
the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c) anterior
view, orifice, (d) posterior view, carina. Scale bars: a–d) 2 mm.
Genus Dichelaspis Darwin, 1852
Dichelasmis Darwin, 1852: 115.
Heptalasmis. Agassiz, 1842: 178.
Octolasmis Gray, 1825: 100. — Pilsbry 1907a: 93.
Diagnosis:
Capitulum with five plates; scutum with two distinct segments, united at rostral angle; carina
generally extending up between terga, terminating downwards as imbedded disc (fork- or cupshaped).
Type species:
Octolasmis warwicki Gray, 1825: 100. — 1830: pl. VI, fig. 16.
Dichelaspis orthogonia Darwin, 1852
Figure 3.6
Dichelaspis orthogonia Darwin, 1852: 130, pl. II, fig. 10a, b.
Dichelaspis versluysi Hoek, 1907: 28, pl. III, figs 8–13.
Octolasmis (Dichelaspis) orthogonia. — Pilsbry 1907a: 94.
Octolasmis orthogonia. — Krüger 1911: 462. — Pilsbry 1911: 70, pl. 11, figs 6–7. — Broch 1922: 279. —
1931: 38, fig. 14. — Nilsson-Cantell 1925: 21, fig. 8. — 1928: 18, fig. 8. — Hiro 1933: 55, fig. 16, pl.
2, fig. 5a, 5. — 1937a: 91, fig.71. — 1937b: 415, fig. 12. — Stubbings 1963: 327, fig. 1. — Dong et
al. 1982: 79. — Zevina et al. 1992: 26, fig. 15. — Chan 2009: 68, fig. 2 B and fig. 17 A–F.
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87
Material examined:
Kei Islands: 4 specimens, MNHN-IU-2019-4864, Stn. CP 27, 05°33’S, 132°51’E, 314–304
m depth, 27 October 1991.
Tanimbar Island: 6 specimens, MNHN-IU-2019-4862, Stn. DW 24, 05°32’S, 132°51’E,
230-243 m depth, 26 October 1991.
Diagnosis:
Capitulum flattened; five plates, separated by thin membrane; scutum consisting of two
distinct segments, with point of junction perfectly calcified; base of carina oval.
Description:
Capitulum and peduncle yellowish. Scutum with basal segment narrow, L-shaped; tergum
triangular, three prominent ridges at scutal margin; base of carina crescent-formed cup. Cirrus I
with anterior ramus (6-segmented) shorter than posterior ramus (8-segmented); cirrus VI with
small caudal appendage. Maxillule notched, three large setae on upper notch; mandible with
four teeth, inferior angle produced into single strong spine; labrum concave, with numerous
teeth. Measurements of specimen: basal diameter of capitulum 1.89 mm; capitular height
8.69 mm; total height 10.47 mm; scutal width 3.5 mm; scutal length 7.16 mm; tergal width
0.80 mm; tergal length 6.53 mm.
Distribution:
Indo-west Pacific: East and South African Coast, Indian Ocean, Australia, Indonesia, Malay
Archipelago, Vietnam, South China Sea, East China Sea, Philippines, Taiwan, South Japan;
attached to hydroid, gorgonians, antipatharians, sea urchin spines, nylon cord, sometimes
fouling; 14–818 m depth (Jones and Hosie 2016). In this study, Dichelaspis orthogonia was
found at Kei Islands and Tanimbar Island, Indonesia.
Type locality:
Unknown.
Remarks
The type locality of this species is unknown. The species was re-discovered in the Malay
Archipelago by the Siboga Expedition in 40–112 m depth (Hoek 1907) and the Albatross
Expedition, off Kagoshima Gulf, Japan, in 87 m depth (Pilsbry 1907d).
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Chapter 3
Figure 3.6. Dichelaspis orthogonia Darwin, 1852 (MNHN-IU-2019-4864). (a) left lateral view capitulum
and peduncle, (b) right lateral view, capitulum and peduncle. Scale bars: a–b) 2 mm.
Genus Glyptelasma Pilsbry, 1907
Glyptelasma Pilsbry, 1907a: 87.
Diagnosis
Capitulum with five approximate, fully calcified plates; scutum quadrangular, umbone
subcentral; carina becoming larger towards base; peduncle short.
Type species:
Megalasma (Glyptelasma) subcarinatum Pilsbry, 1907a: 91, pl. VII, figs 1–5; type locality:
Atlantic Ocean, East of New Jersey, USA.
Glyptelasma gracile (Hoek, 1883)
Figure 3.7
Poecilasma gracile Hoek, 1883: 46, pl. II, figs 2–4.
Megalasma gracile gracilius. — Pilsbry 1907a: 88, pl. V, fig.16, pl. VII, figs 6–9.
Megalasma gracile. — Nilsson-Cantell 1938: 10.
Megalasma (Glyptelasma) gracile. — Zevina 1982: 84, fig. 74.
Glyptelasma gracile. — Jones et al. 2001: 239.
Material examined:
Tanimbar Island: 14 specimens, MNHN-IU-2019-4873, Stn. CP 54, 08°21’S, 131°43’E,
836–869 m depth, 30 October 1991, attached to spine of sea urchin; 1 specimen,
MNHN-IU-2019-4875, Stn. CC 57, 08°19’S, 31°53’E, 603–620 m depth, 31 October 1991;
3 specimens (1 adult, 2 juveniles), MNHN-IU-2019-4876, Stn. CP 73, 08°29’S, 131°33’E,
840-855 m depth, 2 November 1991, attached to spines of sea urchins.
Deep-sea barnacles from the Karubar expedition (1991)
89
Diagnosis:
Capitulum with five plates; carina enlarged, keel-shaped; tergum with basal point truncated;
maxillule notched; caudal appendages with relatively-long spines at tip.
Description:
Capitulum whitish, peduncle brownish. Capitulum compressed, striated, about twice as long
as broad. Scutum with apex pointed; tergum basally truncated, flat, oblong. Carina flat, very
narrow, enlarged, keel-shaped in basal region. Cirrus I with anterior and posterior rami equal
length; cirrus II–VI long, slender; cirrus VI with small caudal appendages. Penis with few long
hairs towards tip. Maxillule notched, with strong teeth on upper side; mandible with four
teeth; labrum with row of small teeth on crest. Measurements of specimen: basal diameter of
capitulum 1.45 mm; capitular height 10.44 mm; total height 13.99 mm; scutal width 4.79 mm;
scutal length 9.01 mm; tergal width 1.87 mm; tergal length 5.07 mm.
Distribution:
West-southwest Pacific: from Australia (Sydney) to Indonesia (Sumbawa) and the Philippines
(northwest Panay Island); East Indian Ocean; attached to glassy spicule of hexactinellid sponge,
fragments of sea urchin tests, spines of cidarids; 395–935 m depth (Jones and Hosie 2016). In
this study, Glyptelasma gracile was found at Tanimbar Island, Indonesia.
Type locality:
Challenger expedition Station 164a (off Sydney, Australia); 34°13’S, 151°38’E; depth 125 m;
bottom: grey ooze (Hoek 1883).
Figure 3.7. Glyptelasma gracile (Hoek, 1883) (MNHN-IU-2019-4875). (a) left lateral view, capitulum and
peduncle, (b) right lateral view, capitulum and peduncle, (c) anterior view, orifice, (d) posterior view,
carina. Scale bars: a–d) 2 mm.
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Chapter 3
Genus Megalasma Hoek, 1883
Megalasma Hoek, 1883: 50. — Pilsbry 1907a: 87.
Diagnosis:
Capitulum with five strong, calcified plates; scutal umbones subcentral; apex of carina
sometimes slightly overlapping basal end of terga, progressively becoming broader towards
base; peduncle very short.
Type species:
Megalasma striatum Hoek, 1883: 51, pl. II, figs 5–9, pl. VII, figs 8–9.
Megalasma striatum Hoek, 1883
Figure 3.8
Megalasma striatum Hoek, 1883: 51, pl. II, figs 5–9, pl. VII, figs 8, 9. — Broch 1931: 270, figs 29–30.
Megalasma (Megalasma) elegans. — Zullo & Newman 1964: 355, fig. 2 a–i.
Megalasma (Megalasma) striatum. — Zevina 1982: 80, fig. 71. — Chan 2009: 66, figs 2A, 16 A–H.
Material examined:
Kei Islands: 1 specimen, MNHN-IU-2019-4868, Stn. CP 09, 05°23’S, 132°29’E, 368–389 m
depth, 23 October 1991; 1 specimen, MNHN-IU-2019-4869, Stn. CP 16, 05°17’S, 132°50’E,
315–349 m depth, 24 October 1991, attached to sea urchin spine; 2 specimens, MNHNIU-2019-4866, Stn. CP 17, 05°15’S, 133°01’E, 439–459 m depth, 24 October 1991.
Tanimbar Island: 1 specimen, MNHN-IU-2019-4867, Stn. CP 46, 08°01’S, 132°51’E, 271–
273 m depth, 29 October 1991; 4 specimens (3 attached to sea urchin spine), MNHNIU-209-4870, Stn. DW 49, 08°00’S, 132°59’E, 206-210 m depth, 29 October 1991; 6
specimens, MNHN-IU-2019-4871, Stn. CP 85, 09°22’S, 131°14’E, 240–245 m depth, 4
November 1991, attached to spines of sea urchin.
Diagnosis:
Capitulum ovate, occludent margin almost same length as carinal margin; capitular plates
heavily calcified, markedly sculptured with radial striations between grooves of growth lines;
peduncle short, covered by capitulum.
Description:
Capitulum white, with five calcified plates, surfaces strongly striated. Scutum with basal margin
rotated; tergum triangular in lateral view; carina with dorsal roof widening apically on either
side of midline groove. Cirrus I with anterior ramus wider than posterior ramus. Maxillule with
three strong setae at upper angle separated by wide notch; mandible with four teeth, lower
angle sharp. Measurements of specimen: basal diameter of capitulum 2.14 mm; capitular
height 9.36 mm; total height 9.36 mm; scutal width 3.81 mm; scutal length 7.28 mm; tergal
width 1.58 mm; tergal length 4.29 mm.
Deep-sea barnacles from the Karubar expedition (1991)
91
Distribution:
West-southwest Pacific, Indo-west Pacific, East coast of Africa, Indian Ocean, north Australia,
Indonesia, Malay Archipelago, East China Sea, South China Sea, Taiwan, Philippines, south
Japan to New Zealand; attached to echinoid spines, antipatharians, gorgonians, glassy spicule
of hexactinellid sponges, corallines; 125–984 m depth (Jones and Hosie 2016). In this study,
Megalasma striatum was found at Kei Islands and Tanimbar Island, Indonesia.
Type Locality:
Philippine Archipelago (Hoek 1883).
Figure 3.8. Megalasma striatum Hoek, 1883 (MNHN-IU-2019-4868). (a) left lateral view, capitulum,
(b) right lateral, capitulum, (c) anterior view, capitulum and peduncle, (d) posterior view, carina. Scale
bars: a–d) 2 mm.
Order Scalpelliformes Buckeridge & Newman, 2006
Scalpelloidea Pilsbry, 1916: 14 (nom. trans. Zevina, 1978).
Scalpelliformes Buckeridge & Newman, 2006: 22.
Family Scalpellidae Pilsbry, 1907
Pollicipedidae Gray, 1825: 100 (part.). — Annandale 1909: 63 (part.).
Lepadidae Darwin, 1852: 8 (part.).
Polyaspidae Gruvel, 1905: 8, 16 (part.; rejected by Pilsbry, 1907a, because family group name not
derived from generic name).
Scalpellinae Pilsbry, 1907a: 3 (part.).
Scalpellidae. — Krüger 1911: 7. — Pilsbry 1916: 4. — Nilsson-Cantell 1921: 162. — Foster 1978: 38. —
Zevina 1978a: 999. — Buckeridge 1983: 27. — Gale 2016: 296.
Diagnosis:
Capitulum with fully or partially calcified plates; peducle with calcareous or phosphorus scales.
According to Gale (2016), the Scalpellidae have a maximum of 14 plates i.e. carina, rostrum,
paired scuta, terga, upper latera, carinolatera, rostrolatera and inferior median latera; and
rarely 13 plates, due to secondary loss of the rostrum.
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Chapter 3
Subfamily Scalpellinae Pilsbry, 1907
Scalpellinae Pilsbry, 1907a: 4. — Zevina 1978a: 1002. — Zevina 1981: 93. — Gale 2016: 296.
Pollicipedidae. — Annandale 1909: 63 (part.).
Diagnosis:
Formerly, the subfamily was characterised by a subapical carinal umbo, inflexed carina
and subapical umbones of the upper and inframedian latus (Zevina 1978a). Gale (2016)
characterised the subfamily by the broad, low, straplike and gently incurved rostrolatus. The
rostrum is broader than high, rectangular, trapezoidal or triangular and its large, triangular,
lateral surfaces contact the interior of the rostrolatus. The articulation surface between the
rostrum and rostrolatus extends over the entire height of both plates.
Type genus:
Scalpellum Leach, 1817: 68.
Genus Diotascalpellum Gale, 2016
Diotascalpellum Gale, 2016: 297.
Diagnosis:
Scalpellines without specialised contact between carina and carinolatus, merely a cresentic
concavity on interior of plate that forms a rim slightly overlapping the upper latus; umbo of
carinolatus upright, only slightly incurved, dorsal margin gently convex; inframedian surface of
carinolatus well demarcated, slightly inset.
Diotascalpellum rubrum (Hoek, 1883)
Figure 3.9
Scalpellum rubrum Hoek, 1883: 91, pl. IV fig. 18. — Pilsbry 1911: 62, text-fig. 1, pl. VIII, figs 1–4. —
Calman 1918b: 122–123. — Broch 1922: 237. — Nilsson-Cantell 1927: 745–747, text-fig. 2. —
1931: 2.
Trianguloscalpellum rubrum. — Zevina 1981: 316–317, fig. 240. — Rosell 1986: 89, text-fig. 1a. — 1991:
22, fig. 1f–g. — Huang 1994: 517. — 2001: 318. — Jones et al. 2001: 254. — Young 2001: 464, fig.
7. — Jones 2007: 292. — Shalaeva & Boxshall 2014: 49, fig. 34.
Diotascalpellum rubrum. — Gale 2016: 297, figs 2A, 6U–Y, 11Q–T, 13K–M, 18A–G.
Material examined:
Kei Islands: 2 specimens, MNHN-IU-2019-4925, Stn. CP 09, 05°23’S, 132°29’E, 368–389
m depth, 23 October 1991; 1 specimen, MNHN-IU-2019-4926, Stn. CP 16, 05°17’S,
132°50’E, 330–350 m depth, 24 October 1991, attached to gorgonian.
Diagnosis:
Capitulum with 14 plates, smooth, white and reddish coloured; carina simply, strongly bowed,
roof slightly convex with umbo at apex; rostrum triangular, distinct; upper latus quadrangular,
large; infra-median latus triangular.
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93
Description:
Capitulum flat, rather broad, not covered by distinct membrane. Scutum with occludent
margin arched, forming with tergal margin a triangular portion projecting over tergum. Tergum
surpassing scutal area with occludent margin almost straight. Upper latus quadrangular, angle
at apex between scutal and tergal margins distinctly projecting over scutum. Rostrum small,
triangular; rostrolatus very low, quadrangular; infra-median latus small, triangular, umbo at
apex; carinal latus larger than other latera with carinal margin arched. Cirrus I with anterior
and posterior rami almost same length; cirrus VI with long caudal appendages. Maxillule not
notched, with large spine on upper side, cutting edge almost straight; mandible with three
large teeth excluding inferior angle. Measurements of two specimens: height of capitulum
12.51–20.57 mm, width 7.39–10.55 mm, thickness 4.09–6.51 mm; length of peduncle 5.10–
7.76 mm, width 4.36–6.62 mm.
Distribution:
Java Sea, Indonesia. Pacific: western central and northwest; Philippines; Kagoshima Sea and
Sagami Bay, Japan; 133–551 m depth (Shalaeva and Boxshall 2014). In this study, Diotascalpellum
rubrum was found at Kei Islands, Indonesia.
Type locality:
Philippines, 12°43’N, 122°10’E.; depth, 180 m and 207 m; bottom, mud (near Luzon) (Hoek
1883).
Figure 3.9. Diotascalpellum rubrum (Hoek, 1883) (MNHN-IU-2019-4925). (a) left lateral view showing
the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c) rostral view
showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 5 mm.
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Chapter 3
Genus Regioscalpellum Gale, 2016
Regioscalpellum Gale, 2016: 298.
Diagnosis:
Concave scalpellines; internal surface upper latus covered by epidermis, short carinal margin
present on carinolatus; carinolatus with incurved umbones, lateral margin strongly convex.
Type species:
Scalpellum regium Thomson, 1873; type locality: Stn 61: Atlantic, Western Central (North
Western Atlantic Basin); 5,210 m depth.
Regioscalpellum moluccanum (Hoek, 1883)
Figure 3.10
Scalpellum moluccanum Hoek, 1883: 104, pl. V figs 3–4; 1907: 56, 85, pl. VII, fig. 13. — Gruvel 1905: 76,
fig. 85. — Nilsson-Cantell 1927: 747, fig. 3.
Arcoscalpellum moluccanum. — Newman & Ross 1971: 60. — Jones et al. 1990: 6, 27. — Jones 1992:
172–175, figs 18–19.
Trianguloscalpellum moluccanum. — Jones 2012: 371, table 2. — Shalaeva & Boxshall 2014: 45, fig. 31.
Regioscalpellum moluccanum. — Gale 2016: 298.
Material examined:
Tanimbar Island: 3 specimens, MNHN-IU-2019-4909, Stn. CP 38, 07°40’S, 132°27’E, 620–
666 m depth, 28 October 1991; 1 specimen, MNHN-IU-2019-4910, Stn. DW 49, 08°00’S,
132°59’E, 206-210 m depth, 29 October 1991; 1 specimen, MNHN-IU-2019–4911, Stn.
CC 57, 08°19’S, 131°53’E, 603–620 m depth, 31 October 1991; 1 specimen, MNHNIU-2019-4912, Stn. CP 52, 08°03’S, 131°48’E, 1244–1266 m depth, 30 October 1991.
Diagnosis:
Capitulum with 14 plates, surface covered by chitinous, almost smooth membrane; carina
simply, not strongly bowed, apex projecting freely, roof not flat; umbo of the carina apical;
upper latus almost triangular.
Description:
Capitulum inflated, subtriangular, apices of plates projecting freely through membrane,
growth lines fairly distinct. Scutum trapezioid, umbo apical. Tergum large, oval, truncated, very
pointed, extending between upper latus and carina. Carina simply, slightly bowed, roof slightly
convex, apex projecting freely. Upper latus quadrangular; rostrum very small, carinate; rostral
latus trapeziform; infra-median latus triangular, moderately small; carinal margins of carinal
latera convex, touching each other at base and below middle of carina. Peduncle stout, slightly
thinner below apex. Cirrus I unequal, anterior ramus oval, posterior ramus more slender; cirrus
VI with long caudal appendages. Maxillule not notched, large spine on upper side, cutting edge
almost straight; mandible with three large teeth excluding inferior angle. Measurements of
five specimens: height of capitulum 7.88–14.89 mm, width 4.81–10.65 mm, thickness 2.15–
5.24 mm; length of peduncle 3.38–9.13 mm, width 2.09–6.04 mm.
Deep-sea barnacles from the Karubar expedition (1991)
95
Distribution:
Pacific, Central and Southwest; Tasman Sea, north-eastern Australia; 788–2,745 m depth
(Jones 2012; Shalaeva and Boxshall 2014). In this study, Regioscalpellum moluccanum was
found at Tanimbar Island, Indonesia.
Type locality:
Banda Sea (4°21’S, 129°7’E); Challenger stn 195; 2,606 m depth: bottom temp. 3°C; substrate
grey ooze (Hoek 1883).
Figure 3.10. Regioscalpellum moluccanum (Hoek, 1883) (MNHN-IU-2019-4909). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
carinal view showing the capitulum and peduncle, (d) rostral view showing the capitulum and peduncle.
Scale bars: a–d) 4 mm.
Genus Scalpellum Leach, 1817
Scalpellum Leach, 1817: 68. — Darwin 1852: 21. — Hoek 1883: 59. — Gruvel 1905: 23. — Pilsbry
1907d: 181. — Tarasov & Zevina 1957: 126. — Zevina 1978a: 1002. — 1981: 94. — Gale 2016: 297.
Strictoscalpellum Broch, 1924: 14.
Diagnosis:
Scalpellines with carinal and scutal umbones subapical; carinolatus with horn-like projection,
often recurved, extending beyond carinal margin; inframedian latus rectangular to slightly
trapezoidal, with low umbo; upper latus rhomboidal with subapical umbo; rostrum rectangular,
pyramidal, with large sub-umbonal surface.
Type species:
Lepas scalpellum Linnaeus, 1767: 1109.
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Chapter 3
Type locality:
Unknown.
Scalpellum sp.
Figure 3.11
Material examined:
Tanimbar Island: 1 specimen, MNHN-UI-2019-4929, Stn. DW 49, 08°00’S, 132°59’E, 206210 m depth, 29 October 1991.
Diagnosis:
Capitulum with 14 fully calcified plates; tergum triangular; inframedian latus broad,
quadrilateral with umbo near to basal; upper latus rhomboidal, umbo sub-apical; carinal
lateral horn-shaped, with angle extending beyond carina.
Description:
Capitulum subtriangular, covered by membrane. Tergum triangular, occludent and basal
margins slightly convex; scutum quadrangular, basal and upper latus margins concave; upper
latus pentagonal; rostrolatus quadrangular; inframedian latus broad, rectangular, umbo close
to basal margin; carinolatus horn-shaped with slightly deep transverse furrows, umbo at basicarinal angle; carina convex, umbo apical. Cirrus I with rami unequal, anterior ramus broader
than posterior ramus; both rami densely covered with long setae. Maxilla subtriangular, with
dense long setae; maxillule notched, with numerous setae; mandible with three teeth, inferior
angle with large cuspidate setae. Measurements of specimen: height of capitulum 15.90 mm,
width 10.29 mm, thickness 4.58 mm; length of peduncle 6.97 mm, width 5.67 mm.
Distribution:
In this study, Scalpellum sp. was found in Tanimbar Island, Indonesia.
Figure 3.11. Scalpellum sp. (MNHN-UI-2019-4929). (a) left lateral view, capitulum and peduncle, (b)
right lateral view, capitulum and peduncle, (c) rostral view, capitulum and peduncle, (d) carinal view.
Scale bars: a–d) 7 mm.
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97
Scalpellum stearnsi Pilsbry, 1890
Figure 3.12
Scalpellum magnum Darwin, 1852: 18, pl. I, fig. 1.
Scalpellum calcariferum Fischer, 1891: 116.
Scalpellum stearnsii var. gemina and var. robusta Hoek, 1907: 69, pl. VI, figs 2–3, 8–12.
Scalpellum stearnsii var. inerme Annandale, 1916a: 293.
Scalpellum stearnsii forma typica Broch, 1931: 16.
Scalpellum stearnsii Pilsbry, 1890a: 96. — 1890b: 441, pl. IV, figs 1–5. — 1907a: 14, pl. 4, figs 1-5. —
Nilsson-Cantell, 1921: 175. — 1934: 33. — Broch, 1922: 235, fig. 6. — Hiro, 1933: 22, fig. 4, pl. 1,
figs 5, 5a. — 1939a: 237. — Zevina 1981: 98, fig. 68. — Rosell 1991: 15. — Jones 1992: 146, figs 1,
2. — Liu & Ren 2007: 226, fig. 95. — Chan et al. 2009b: 89, figs 73–76. — Chan et al. 2010: 24, figs
2C, 18A–G.
Scalpellum stearnsi: Gruvel, 1905: 44, fig. 46. — Gale 2016: fig. 5, table 1.
Material examined:
Tanimbar Island: 1 specimen, MNHN-IU-2019-4888, Stn. CP 71, 08°38’S, 131°44’E, 477–
480 m depth, 2 November 1991; 1 specimen, MNHN-IU-2019-4889, Stn. CC 58, 08°19’S,
132°02’E, 457–461 m depth, 31 October 1991; 6 specimens, MNHN-IU-2019–4890, Stn.
CC 42, 07°53’S, 132°42’E, 350–354 m depth, 28 October 1991; 3 specimens, MNHNIU-2019-4891, Stn. CP 69, 08°42’S, 131°53’E, 356–368 m depth, 2 November 1991; 5
specimens, MNHN-IU-2019-4892, Stn. CC 41, 07°45’S, 132°42’E, 393-401 m depth, 28
October 1991; 5 specimens, MNHN-IU-2019-4893, Stn. CP 59, 08°20’S, 132°11’E, 399405 m depth, 31 October 1991, 2 specimens attached to glass rope sponge.
Kei Islands: 8 specimens, MNHN-IU-2019-4894, Stn. CP 06, 05°49’S, 132°21’E, 287-298 m
depth, 22 October 1991.
Diagnosis:
Capitulum rhomboidal, compressed; carina strongly angled at umbo; scutum large, umbo at
tergo-occludent angle; tergum triangular; inframedian latus pentagonal; carinolateral hornshaped.
Description:
Capitulum with 14 fully calcified plates. Scutum large, longer than broad, apex slightly projecting
over tergum; tergum large, triangular, apex erect, slightly recurved. Carina distinctly bent near
middle, umbo distant from apex. Cirrus I with anterior ramus oval (12-segments), posterior
ramus slender, long (15-segments); cirri II–VI slender, long, rami almost equal length; cirrus
VI with short caudal appendages (six segments). Maxillule not notched, with numerous setae;
mandible with six teeth excluding inferior angle. Measurements of five specimens: height of
capitulum 31.69–52.00 mm, width 28.20–40.11 mm, thickness 11.19–18.56 mm; peduncle
length 28.29–86.59 mm, width 11.39–17.94 mm.
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Chapter 3
Distribution:
Indo-west Pacific: Indian Ocean, off Nicobar Island; Indonesia: Java Sea, Bali Straits; north west
& north east Australia; Malay Archipelago; Celebes; Sulu Arch.; Sulu Sea; South China Sea; East
China Sea; Philippines; Taiwan; east coast of south Japan, Sagami Bay, off Hondo; attached to
mollusc shells, anchor filaments of hexactinellid sponges, telegraph cables, carapaces of crabs,
stones; 146–2117 m depth (Jones and Hosie 2016). In this study, Scalpellum stearnsi was found
at Tanimbar Island and Kei Islands, Indonesia.
Type locality:
East coast of Japan, between the Bay of Tokyo and the Inland Sea (Jones 1992).
Remarks:
For the first time, Scalpellum stearnsi was found in Japan and described by Pilsbry (1890).
During the Siboga expedition (1899), S. stearnsi was collected from different locations in
the Malay Archipelago with the depths varying from 204 m to 450 m. Hoek (1907) found
intraspecific variations of the shell plate morphology. He then divided S. stearnsi into two
groups, i.e. variety robusta and var. gemina, which differed in the shape of the tergum. The
species S. stearnsi in this study belongs to the group of var. gemina because of the V-shaped
tergum.
Scalpellum stearnsi has a low period of larval development (Ozaki et al. 2008) and a
slow growth rate (Yusa et al. 2018). This can result in the broad geographical distribution of
this species. Recently, Lin et al. (2020) examined the diversity and genetic differentiation of
populations of S. stearnsi from the East China Sea, West Philippine Basin, Sulu Sea and Caroline
Trenches, which resulted in four distinct clades of S. stearnsi.
Figure 3.12. Scalpellum stearnsi Pilsbry, 1890 (MNHN-IU-2019-4888). (a) left lateral view, capitulum
and peduncle, (b) right lateral view, capitulum and peduncle, (c) rostral view, capitulum and peduncle,
(d) carinal view. Scale bars: a–d) 14 mm.
Deep-sea barnacles from the Karubar expedition (1991)
99
Subfamily Meroscalpellinae Zevina, 1978
Meroscalpellinae Zevina, 1978b: 1343.
Diagnosis:
Capitulum with 14 or 13 plates, reduced in differing stages or proportions; carina with two
umbo positions; females considered rarer than hermaphrodites; males saclike, usually without
plates, rarely with two or four reduced plates.
Type genus:
Meroscalpellum Zevina 1978b: 1346.
Genus Alcockianum Zevina, 1978
Alcockianum Zevina, 1978b: 1345.
Diagnosis:
Plates strongly reduced; partly obscured under heavy cuticle; tergum double branched,
depressed at base; scutum triangular; upper lateral triangular; midlateral very small, umbo
apical; caudal appendages very long; hermaphrodites.
Type species:
Scalpellum alcockianum Annandale, 1905: 82; type locality: Gulf of Manaar and Andaman Sea.
Alcockianum persona (Annandale, 1916)
Figure 3.13
Scalpellum persona Annandale, 1916a: 295, pl. IV, fig. 3; pl. V, figs 7, 8; pl. VI, figs 3–5. — Calman 1918b:
120. — Nilsson-Cantell 1934: 44, pl. 5, fig. 2.
Graviscalpellum persona Foster, 1980: 527, figs 1e, 2.
Alcockianum persona Zevina, 1978b: 1345. — 1981: 150–151, fig. 107. — Jones 1992: 151–154, figs 4, 5.
Material examined:
Tanimbar Island: 2 specimens, MNHN-IU-2019-4903, Stn. CP 71, 08°38’S, 131°44’E,
477–480 m depth, 2 November 1991; 5 specimens, MNHN-IU-2019-4904, Stn. CC 21,
05°14’S, 133°00’E, 688–694 m depth, 25 October 1991 attached to glass rope sponge;
1 specimen, MNHN-IU-2019-4905, Stn. CC 40, 07°46’S, 132°31’E, 443–468 m depth, 28
October 1991; 5 specimens, MNHN-IU-2019-4906, Stn. CC 57, 08°19’S, 131°53’E, 603–
620 m depth, 31 October 1991.
Diagnosis:
Capitulum with plates buried or obscure; plates reduced in size; tegum reduced, forming fourpointed star; scutum small; peduncle armed with distinct circles of large, calcareous scales.
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Description:
Capitulum brownish, large, ovoid, inflated, with 13 capitular plates, including a vestigial
rostrum, plates embedded and mostly concealed by thick, opaque membrane. Scutum small,
widely separated from all remaining plates except tergum, margins not excavated or deeply
concave; tergum reduced in form as four-pointed star, with two rays greatly and two rays slightly
produced. Carina reduced in size, apex approaching terga, widely separated from remaining
plates. Peduncle cylindrical, similar length to capitulum, with large calcareous scales arranged
in alternating rows. Cirrus I with anterior ramus oval (8-segments), posterior ramus slender,
long (12-segments); cirri II–VI slender, long, rami almost equal length; cirrus VI with caudal
appendages; caudal appendages 1/3 length of cirrus VI, 15-segmented, tapering distally. Penis
rather short, smooth, pointed. Maxilla bilobed, dense setae on margin. Maxillule relatively
large, with broad, shallow excavation on lower margin occupying more than half margin,
remainder of margin obliquely subtruncate; mandible with three main teeth in addition to
inner angle, which is variously divided, broad as a whole. Measurements of five specimens:
height of capitulum 17.71–35.06 mm, width 12.91–28.06 mm, thickness 4.96–18.20 mm;
length of peduncle 13.42–28.67 mm, width 6.66–10.79 mm.
Distribution:
Indonesian Seas, eastern Australia, New Zealand; 109–915 m depth (Jones 1992). In this study,
Alcockianum persona was found at Tanimbar Island, Indonesia.
Type Locality:
Timor Sea (10°22’30”S, 120°7’30”E); 109–366 m depth: bottom temp. 13.1°C (Jones 1992).
Figure 3.13. Alcockianum persona (Annandale, 1916) (MNHN-IU-2019-4903). (a) left lateral view,
capitulum and peduncle, (b) right lateral view, capitulum and peduncle, (c) rostral view, capitulum and
peduncle, (d) carinal view. Scale bars: a–d) 9 mm.
Deep-sea barnacles from the Karubar expedition (1991)
101
Genus Annandaleum Newman & Ross, 1971
Annandaleum Newman & Ross, 1971: 122. — Zevina, 1978b: 1346.
Diagnosis:
Capitulum of female with 14 partially calcified plates; tergum in shape of inverted V; scutum
with moderately long apicolateral arm, 1/4 to 1/2 length of tergal margin; basal margin of
scutum entire; upper latus pentagonal to triangular or subrectangular, with or without short
depending arm; carinolatus as high as or slightly higher than wide, umbo at basicarinal angle;
inframedian latus higher than wide, vase-shaped, umbo submedial to basal; rostrolatus wider
than high, commonly less than twice height of inframedian latus; caudal appendage relatively
long.
Type species:
Scalpellum subflavum Annandale, 1906.
Annandaleum japonicum (Hoek, 1883)
Figure 3.14
Scalpellum japonicum Hoek, 1883: 67, pl. III, figs 9–10. — Annandale 1906: 131, fig. 3. — Pilsbry 1911:
66, pl. II, figs 1–3. — Weltner 1922: 69, taf. II, fig. 4. — Hiro 1937 b: 392. — Tarasov & Zevina, 1957:
144, figs 46–47. — Zevina 1969: 68. — 1970: 257–259, figs 5–6.
Scalpellum chitinosum Hoek, 1907: 73.
Scalpellum curiosum Hoek, 1907: 49.
Scalpellum japonicum biramosum Pilsbry, 1911: 68, fig. 4. — Weltner 1922: 69, taf. II, fig. 5. — NilssonCantell 1938: 7.
Scalpellum japonicum metapleurum Pilsbry, 1907b: 360.
Annandaleum japonicum biramosum Chan et al., 2009b: 98–99, fig. 80. — Chan et al. 2010: 17–18, figs
2A, 14, 15.
Annandaleum japonicum. — Newman & Ross 1971: 122. — Zevina 1981: 166–167. — Jones et al. 2001:
251. — Young 2001: 465, fig. 8. — 2007: 23, fig. 22.
Material examined:
Tanimbar Island: 4 specimens, MNHN-IU-2019-4932, Stn. CP 53, 08°18’S, 131°41’E,
1026–1053 m depth, 30 October 1991.
Diagnosis:
Capitulum with 14 partly calcified plates; carina with flat roof; apex of tergum curved towards
carina; rostral latus trapezoidal in shape; peduncle short.
Description:
Capitulum yellowish, with 14 plates separated by broad, chitinous spaces. Peduncle short,
curved, scales large, not numerous. Scutum long, narrow; umbo apical, divided into two
segments, occludent segment large, increasing in width from upper to lower part, other
segment very narrow. Tergum divided into occludent and carinal segments, with triangular,
chitinous portion between, umbo apical, distinctly recurved. Cirrus I unequal, anterior ramus
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Chapter 3
oval, posterior ramus slender long; cirrus VI with caudal appendages. Maxillule slightly
notched, two long and one short setae on upper side; mandible with three teeth excluding
inferior angle; labrum cutting edge straight, very fine teeth on cutting edge. Measurements
of the four specimens: height of capitulum 9.56–12.73 mm, width 4.67–7.13 mm, thickness
1.85–3.21 mm; length of peduncle 3.37–4.77 mm, width 1.83–3.27 mm.
Distribution:
Eastern Indian Ocean; Northwest and Western Central Pacific; Malay Archipelago; Japan;
Taiwan; Indonesia; attached to shell of gastropod, gorgonians, rocks; 805–6,810 m depth
(Jones and Hosie 2016). In this study, Annandaleum japonicum was found at Tanimbar Island,
Indonesia.
Type locality:
Pacific, Northwest (off Nagoya, south of Japan); 1,017 m depth (Shalaeva and Boxshall 2014).
Figure 3.14. Annandaleum japonicum (Hoek, 1883) (MNHN-UI-2019-4932). (a) left lateral view showing
the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c) rostral view
showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 2 mm.
Annandaleum laccadivicum (Annandale, 1906)
Figure 3.15
Scalpellum laccadivicum Annandale, 1906b: 393. — 1908: pl. I, figs 3, 4. — 1913: 235; 1916b: 129, pl.
vii, fig. 6. — Calman, 1918b: 124. — Hiro, 1933: 31, text figs 7, 8, pl. I, figs 11–11b. — 1937b: 392.
— Stubbings 1936: 26. — Nilsson-Cantell 1938: 25.
Scalpellum subflavum Annandale, 1906b: 397. — Newman & Ross 1971: 122.
Deep-sea barnacles from the Karubar expedition (1991)
103
Scalpellum polymorphum Hoek, 1907: 80, pl. 7, figs 9–11. — Weltner 1922: 72.
Scalpellum molliculum Pilsbry, 1911: 68, pl. 10, figs 4, 5.
Annandaleum laccadivicum Rosell, 1991: 16, fig 1a. — Zevina 1981: 170, fig. 121. — Jones et al. 2001:
251. — Chan et al. 2009b: 102–103.
Material examined:
Tanimbar Island: 1 specimen, MNHN-IU-2019-4931, Stn. CP 54, 08°21’S, 131°43’E, 836–
869 m depth, 30 October 1991, attached to gastropod shell.
Diagnosis:
Capitulum regularly oval; carina simply bowed; tergum almost triangular; scutum subtriangular;
upper latus large, perfectly calcified part irregularly triangular, constricted above; rostral latus
quadrangular; peduncle at least half as long as capitulum.
Figure 3.15. Annandaleum laccadivicum (Annandale, 1906) (MNHN-IU-2019-4931). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
rostral view showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 5 mm.
Description:
Capitulum compressed; 13 plates completely covered by fine, hairless membrane. Peduncle
half length of capitulum, stout, cylindrical, armed with small, transversely elongated plates.
Scutum subtriangular, lateral margin excavated with tooth above excavation blunt, short,
simple; tergum almost triangular, scutal margin excavated, but not very boldly, occludent
margin slightly, regularly convex outwards. Carina simply bowed, umbo subterminal, in
contact with terga above or just entering between them. Cirrus I unequal, anterior ramus oval,
posterior ramus slender, long; cirri II–VI slender, long, rami almost equal lengths; cirrus VI with
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Chapter 3
long, slender caudal appendages. Maxillule slightly notched, two major setae on upper side;
mandible with four teeth. Measurements of specimen: height of capitulum 18.88 mm, width
11.41 mm, thickness 6.30 mm; length of peduncle 11.45 mm, width 4.89 mm.
Distribution:
Gulf of Oman, Arabian Sea, Sri Lanka, Japan (Chan et al. 2009b). In this study, Annandaleum
laccadivicum was found at Tanimbar Island, Indonesia.
Type locality:
Laccadive Sea, 2,077 m depth; on living shells of Dentalium (Annandale 1906).
Annandaleum lambda (Annandale, 1910)
Figure 3.16
Scalpellum lambda Annandale, 1910b: 115. — 1916a: pl. VII, figs 6, 6a, pl. VIII, figs 12–15.
Scalpellum longius Annandale, 1913: 234.
Annandaleum lambda. — Newman & Ross, 1971: 122. — Jones, 1992: 154, fig. 6.
Material examined:
Tanimbar Island: 10 specimens, MNHN-IU-2019-4915, Stn. CP 52, 08°03’S, 131°48’E,
1244–1266 m depth, 30 October 1991; 1 specimen, MNHN-IU-2019-4913, Stn. CP
53, 08°18’S, 131°41’E, 1026–1053 m depth, 30 October 1991; 1 specimen, MNHNIU-2019-14, Stn. CP 89, 08°39’S, 131°08’E, 1058-1084 m depth, 5 November 1991,
attached to pumice.
Diagnosis:
Capitulum narrow; carinal margin strongly curved; laterally compressed; bearing fourteen
imperfectly formed, thin, smooth, translucent plates, eight formed like a Greek lambda.
Description:
Capitulum with 14 plates. Peduncle shorter than capitulum, cylindrical, rather slender,
expanded at base. Tergum lambda-shaped, with prominent tooth on carinal margin just above
point where apex of carina approaches it. Scutum shaped similarly, but occludent branch much
stouter, with vertical ridge running nearer to lateral than occludent margin. Cirrus I unequal,
anterior ramus oval, posterior ramus slender, long; cirri II–VI slender, long, with rami almost
equal length; cirrus VI with long, slender caudal appendages. Maxillule not notched, two major
setae on upper side; mandible with four teeth including inner angle. Measurements of five
specimens: height of capitulum 16.61–24.27 mm, width 8.73–14.49 mm, thickness 3.17–6.31
mm; length of peduncle 5.54–15.27 mm, width 3.73–6.26 mm.
Deep-sea barnacles from the Karubar expedition (1991)
105
Distribution:
Indo-Pacific from off Zanzibar, Indian Ocean, eastern Australia; Sumbawa, Indonesia; Malay
Arch.; SW of Calatagan Pt, Philippines; S Japan (S of Honda I.), SW Pacific; attached to shells of
bivalves; 234–2077 m depth (Jones and Hosie 2016). In this study, Annandaleum lambda was
found at Tanimbar Island, Indonesia.
Type locality:
Eastern Indian Ocean (13°54’15”N, 94°02’15”E), Investigator Stn. 372; 1,176 m depth (Jones
1992).
Figure 3.16. Annandaleum lambda (Annandale, 1910) (MNHN-IU-2019-4915). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
rostral view showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 6 mm.
Genus Litoscalpellum Newman & Ross, 1971
Litoscalpellum Newman & Ross, 1971: 108. — Zevina 1978b: 1344. — Liu & Ren 1985: 196. — 2007:
228.
Diagnosis:
Capitulum with 14 plates, mostly not reduced; tergum with straight basal margin or shallow
notch; scutum with straight, smooth basal margin; upper latus triangular or elongate,
commonly with slightly hollowed-out basal margin, rarely with deep notch; inframedian latus
narrow, triangular or quadrilateral, umbo apical; caudal appendages present.
Type species:
Litoscalpellum fissicarinatum Newman & Ross, 1971: 108, pl. XC, text-fig. 55.
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Chapter 3
Litoscalpellum juddi (Calman, 1918)
Figure 3.17
Scalpellum juddi Calman, 1918b: 116, text-figs 5–7.
Scalpellum regulus Calman, 1918b: 113, text-fig. 4.
Litoscalpellum juddi Zevina, 1981:135–136, fig. 94.
Material examined:
Tanimbar Island: 5 specimens, MNHN-IU-2019-4896, stn. CC 56, 08°16’S, 131°59’E, 549552 m depth, 31 October 1991; 1 specimen, MNHN-IU-2019-4898, stn. CP 71, 08°38’S,
131°44’E, 477–480 m depth, 2 November 1991; 6 specimens, MNHN-IU-2019-4901, stn.
CP 72, 08°36’S, 131°33’E, 676-699 m depth, 2 November 1991.
Diagnosis:
Capitulum compressed, oval, notched above; surface covered with very short velvety
pubescence, areas of valves defined by grooves, lines of growth strongly marked; carina
flattened.
Figure 3.17. Litoscalpellum juddi (Calman, 1918) (MNHN-UI-2019-4898). (a) left lateral view showing
the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c) rostral view
showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 11 mm.
Description:
Capitulum with 14 plates, cartilaginous thick. Terqum irregularly triangular, apex projecting
above carina, recurved. Scutum triangular, basal width more than half height, apex overlapping
tergum. Carina evenly curved, apex projecting freely for short distance, touching or entering
between terga, its base rounded, widely separated from carinal latera; roof strongly convex.
Upper latus triangular with base convex. Rostrum triangular; inframedian latus small,
triangular, base deeply embedded. Peduncle with large transverse plates, not overlapping
(widely spaced). Cirrus I unequal, anterior ramus oval, posterior ramus slender, long; cirrus VI
Deep-sea barnacles from the Karubar expedition (1991)
107
with caudal appendages. Maxillule with oral edge sinuous, a large seta at its proximal third,
another at its distal end; mandible with four teeth. Measurements of five specimens: height
of capitulum 16.94–39.25 mm, width 11.39–28.28 mm, thickness 5.20–19.89 mm; length of
peduncle 15.02–52.02 mm, width 5.63–16.87 mm.
Distribution:
In this study, Litoscalpellum juddi was found at Tanimbar Island, Indonesia.
Type locality:
11°0’S, 121°30’E (Java-Australia), 720 m depth (Calman 1918b).
Litoscalpellum recurvirostrum (Hoek, 1883)
Figure 3.18
Scalpellum recurvirostrum Hoek, 1883: 77–79, pl. III, figs 11–12, pl. VIII, figs 9–10.
Arcoscalpellum recurvirostrum. — Newman & Ross 1971: 79–80, fig. 39.
Litoscalpellum recurvirostrum. — Zevina 1974: 214. — 1981: 127–128, fig. 88. — Shalaeva & Boxshall
2014: 13, fig. 6.
Material examined:
Kei Islands: 1 specimen, MNHN-IU-2019-4934, Stn. CP 05, 05°49’S, 132°18’E, 296–299
m depth, 22 October 1991; 1 specimen, MNHN-IU-2019-4936, Stn. CP 09, 05°23’S,
132°29’E, 368–389 m depth, 23 October 1991.
Tanimbar Island: 4 specimens, MNHN-IU-2019-4935, Stn. CP 83, 09°23’S, 131°00’E,
285–297 m depth, 4 November 1991; 16 specimens, MNHN-IU-2019-4937, Stn. CP 69,
08°42’S, 131°53’E, 356–368 m depth, 2 November 1991.
Diagnosis:
Capitulum plates separated, covered by fine membrane; carina slightly angularly bent; upper
latus trapeziform; inframedian latus triangular; peduncle with scattered, calcareous scales.
Description:
Capitulum with 14 plates, lines of growth not distinct, plates separated by broad, membranous
interspaces. Scutum elongate quadrangular, broader at base than in upper half; umbo apical,
slightly projecting outwards. Tergum triangular; carina bowed; upper latus trapeziform;
inframedian latus very small, triangular. Peduncle cylindrical with numerous calcareous scales
scattered over surface. Cirrus I with rami unequal, anterior ramus oval, posterior ramus
slender, long, segments very hairy. Maxilla bilobed; maxillule notched, two large setae above
notch; mandible with three teeth, inferior angle pectinated. Measurements of five specimens:
height of capitulum 16.15–26.05 mm, width 9.24–16.59 mm, thickness 4.45–8.18 mm; length
of peduncle 7.25–20.90 mm, width 4.13–8.95 mm.
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Chapter 3
Distribution:
Indian Ocean, Antarctic and Southern (South of the Kerguelen Islands); known depth range
195 to 274 m (Shalaeva and Boxshall 2014). In this study, Litoscalpellum recurvirostrum was
found at Kei Islands and Tanimbar Island, Indonesia.
Type locality:
Between Kerguelen and Heard Islands; 52°4’S, 71°22’E; depth: 270 m; bottom temperature:
1.8°C; bottom: rocks (Hoek 1883).
Figure 3.18. Litoscalpellum recurvirostrum (Hoek, 1883) (MNHN-UI-2019-4934). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
rostral view showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 6 mm.
Litoscalpellum walleni Newman & Ross, 1971
Figure 3.19
Litoscalpellum walleni Newman & Ross, 1971: 116, pl. X E text-fig. 60.
Material examined:
Tanimbar Island: 1 specimen, MNHN-IU-2019-4927, Stn. CP 91, 08°44’S, 131°05’E, 884–
891 m depth, 5 November 1991.
Diagnosis:
Capitulum with 14 plates; inframedian latus triangular, very small, narrow; carina with flat
roof; rostrum minute; scutum lacking prominent apicolateral arm; maxilla subtriangular,
slightly trilobate; maxilulle slightly notched with teeth diverging laterally from cutting edge;
caudal appendage multi-articulate, slightly shorter than first segment of cirrus VI.
Deep-sea barnacles from the Karubar expedition (1991)
109
Description:
Capitulum elongate, higher than broad; plates ornamented with growth lines. Carina bowed,
roof slightly convex, bounded by angles. Tergum triangular; scutum with lateral margin concave;
upper latus roughly quadrangular; carinal latus higher than wide; rostral latus quadrangular,
slightly broader than high; rostrum minute, narrow, essentially rectangular. Cirrus I unequal,
anterior ramus shorter, broader than posterior ramus; both rami densely covered with long
setae. Mandible with four teeth including inferior angle, inferior angle serrate along basal
margin with four spines. Measurements of specimen: height of capitulum 14.56 mm, width
8.09 mm, thickness 2.50 mm; length of peduncle 7.52 mm, width 3.89 mm.
Distribution:
Southeast Pacific Ocean (Newman and Ross 1971). In this study, Litoscalpellum walenni was
found at Tanimbar Island, Indonesia.
Type locality:
Northeast of Peter I Island, southeast Pacific Ocean; 65° 50› S, 88° 56› W; depth: 4,502 m
(Newman and Ross 1971).
Figure 3.19. Litoscalpellum walleni Newman & Ross, 1971 (MNHN-UI-2019-4927). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
rostral view showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 4 mm.
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Chapter 3
Subfamily Arcoscalpellinae Zevina, 1978
Arcoscalpellinae Zevina, 1978b: 1346. —1981: 184. — Liu & Ren 2007: 238.
Diagnosis:
Capitulum with plates packed very closely; carina slightly convex; umbo of scutum and tergum
apical.
Type genus:
Arcoscalpellum Hoek, 1907: 57.
Genus Amigdoscalpellum Zevina, 1978
Arcoscalpellum Hoek, 1907: 85 (in part). — Newman & Ross 1971: 42 (part).
Amigdoscalpellum Zevina, 1978b: 1349. — Foster 1980: 527. — Liu & Ren 1985: 206. — 2007: 251.
Diagnosis:
Capitulum of female or hermaphrodite with 13 or 14 completely calcified plates; scutum and
tergum with umbones apical; carina evenly bent, umbo apical or subapical; middle latera
narrowly triangular, baton-like, small, umbo apical, not reaching upper latera; carinolatera
typically with umbo orientated in middle part of carinal area, not extending beyond rim of
capitulum; caudal appendages usually uni-articulate, but possibly absent or extended to seven
segments; basically females with dwarf males.
Type species:
Scalpellum manum Zevina, 1973: 843, figs 1–7; type locality: Indian Ocean, 12°18’S, 112°43’E.
Amigdoscalpellum costellatum (Withers, 1935)
Figure 3.20
Scalpellum elongatum Hoek 1883: 93, pl. IV, figs 8–9. — Nilsson-Cantell 1928: 8, fig. 4. — 1931: 3, textfig.1. — 1938: 7.
non Scalpellum (Arcoscalpellum) elongatum. — Steenstrup, 1837: 409.
Scalpellum (Scalpellum) elongatum. — Stubbings 1936: 25, text-fig. 10.
Scalpellum (Arcoscalpellum) costellatum Withers, 1935: 279.
Arcoscalpellum buccinum Newman & Ross, 1971: 55, pl. VIII, figs 22–23.
Arcoscalpellum costellatum. — Foster 1978: 56, pl. 7B–C, fig. 33.
Amigdoscalpellum costellatum. — Foster 1980: 527–529, fig. 3J. — Zevina 1981: 270–271, fig. 204.
— Jones et al. 1990: 5 — Jones 2012: 371, 376 — Poltarukha 2013: 52–53, fig. 1. —Shalaeva &
Boxshall 2014: 16, fig. 9.
Material examined:
Tanimbar Island: 2 specimens, MNHN-IU-2019-4922, Stn. CC 21, 05°14’S, 133°00’E, 688–
694 m depth, 25 October 1991, attached to glass rope sponge.
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111
Diagnosis:
Capitulum triangular; plates ornamented with radial ridges, covered by sparsely hirsute
integument; carina with roof deeply grooved; scutum with apex overlapping tergum;
inframedian latus triangular, very small.
Desciption:
Capitulum yellowish, with 13 fully calcified plates. Peduncles short with scales slightly overlapping
in the middle part. Scutum with pit for complemental males, above shallow pit for adductor muscle.
Carina wide in lower part, ribbed in upper part. Upper latus with straight sides; rostrum appearing
externally as inverted triangle. Cirrus I unequal, anterior ramus oval, posterior ramus slender, long;
cirrus VI with very short caudal appendages. Maxillule with notch between two or three stout setae
at upper angle, group of more slender setae on cutting edge; mandible with three teeth excluding
inferior angle; labrum cutting edge slightly concave, numerous pointed teeth on cutting edge.
Measurements of two specimens: height of capitulum 12.44–13.88 mm, width 6.97–7.52 mm,
thickness 2.77–3.47 mm; length of peduncle 2.99–3.15 mm, width 2.94–3.24 mm.
Distribution:
Indian Ocean, Eastern and Western; Pacific, Southwest and Western Central; Atlantic,
Southeast and Eastern central; 110–2,397 m depth (Shalaeva and Boxshall 2014). In this study,
Amigdoscalpellum costellatum was found at Tanimbar Island, Indonesia.
Type locality:
1. Station 135: Island of Tristan da Cunha; depth: 110 m, 137 m, 183 m, 274 m, 1,006 m, 1,829
m, 2,012 m; bottom: rocky, shells. 2. Station 164a: off Sydney, 34º13’S, 151°38’E; depth: 750
m; bottom: grey ooze. 3. Station 169: off East Cape, Auckland, 37°34’S, 179°22’E; depth: 1,280
m; bottom temperature: 4.2°C; bottom: grey ooze (Hoek 1883).
Figure 3.20. Amigdoscalpellum costellatum (Withers, 1935) (MNHN-IU-2019-4922). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
carinal view, (d) rostral view showing the capitulum and peduncle. Scale bars: a–d) 3 mm.
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Chapter 3
Amigdoscalpellum tenue (Hoek, 1883)
Figure 3.21
Scalpellum tenue Hoek, 1883: 119, pl. IV, figs 20–21, pl. X, fig. 6. — Gruvel 1912: 345. — Nilsson-Cantell
1938: 8.
non Scalpellum tenue Annandale, 1906a: 142.
Amigdoscalpellum tenue. — Zevina 1981: 292–293, fig. 220 (2). — Shalaeva & Boxshall 2014: 17, fig. 10.
Material examined:
Kei Islands: 1 specimen, MNHN-IU-2019-4921, Stn. CP 20, 05°15’S, 132°59’E, 769–809 m
depth, 25 October 1991.
Diagnosis:
Capitulum with 13 fully calcified plates; carina slightly bowed, umbo at apex; upper latus
trapeziform; inframedian latus small, narrow; carinal latus not projecting beyond carina;
peduncle short, cylindrical.
Figure 3.21. Amigdoscalpellum tenue (Hoek, 1883) (MNHN-IU-2019-4921). (a) left lateral view showing
the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c) carinal view
showing the capitulum and peduncle, (d) rostral view showing the capitulum and peduncle. Scale bars:
a–d) 2 mm.
Description:
Capitulum yellowish, elongate-oval shape; surface with distinct lines of growth. Carina large,
simply bowed. Scutum with umbo at apex, slightly recurved, projecting slightly over tergum;
tergum triangular, stout, broad, apex recurved, scutal margin almost straight. Upper latus
quadrangular, apex slightly projecting over scutum. Rostral latus quadrangular, scutal and basal
margins parallel. Carinal latus quadrangular, carinal margin almost straight. Cirrus I unequal,
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113
anterior ramus oval, posterior ramus more slender. Maxillule with notch between two or three
stout setae at upper angle, a group of more slender setae on cutting edge; mandible with
three teeth excluding inferior angle; labrum with numerous blunt teeth on straight, cutting
edge. Measurements of specimen: height of capitulum 7.26 mm, width 3.74 mm, thickness
1.40 mm; length of peduncle 3.00 mm, width 1.99 mm.
Distribution:
Indian Ocean, Antarctic and Southern (North East of Prince Edward Island); known depth
2,516 m (Shalaeva and Boxshall 2014). In this study, Amigdoscalpellum tenue was found at Kei
Islands, Indonesia.
Type locality:
46°46’S, 45°31’E; depth: 2,475 m; bottom temperature: 1.5°C; bottom: globigerina ooze (Hoek
1883).
Genus Catherinum Zevina, 1978
Acroscalpellum Hoek, 1907: 85. — Newman & Ross 1971: 42 (part). — Liu & Ren 1985: 202.
Catherinum Zevina, 1978b: 1348.
Diagnosis:
Capitulum with 13 or 14 plates; inframedian latus very narrow, bacilliform, umbo sub-medial;
umbo of upper latus apical or sub-apical; carinal latus broad, umbo sub-basicarinal, not
extending beyond carinal margin; caudal appendages long.
Type species:
Scalpellum recurvitergum Gruvel, 1902; type locality: Atlantic Ocean, SW of Azores.
Catherinum rossi (Rao & Newman, 1972)
Figure 3.22
Arcoscalpellum rossi Lakshmana Rao & Newman, 1972: 82, fig. 7.
Catherinum rossi. — Zevina 1978b: 1348. — Liu & Ren 1985: 203, fig. 12, pl. 2, figs 11–14. — 2007: 250,
fig. 108. — Chan et al. 2009b: 113, figs 92–94.
Material examined:
Tanimbar Island: 1 specimen, MNHN-IU-2019-4933, Stn. DW 02, 05°47’S, 132°13’E, 209–
240 m depth, 22 October 1991.
Diagnosis:
Capitulum smooth, elongate, 14 fully calcified plates; inframedia latus rectangular, not
constricted; rostrum large, ovo-triangular; caudal appendages short.
Description:
Capitulum long, narrow, sparsely covered with hairs, plates separated by narrow, chitinous
interspaces, marked with growth lines. Occludent margin strongly convex; carinal margin
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Chapter 3
irregularly straight; apex slightly retroverted towards carinal side. Carina long, simply bowed;
roof flat; parietes well developed towards distal half of plate. Tergum triangular, occludent
margin short, convex, scutal and basal margins almost straight, carinal margin concave. Scutum
with umbo apical, overlapping occludent margin of tergum. Upper latus triangular; carinal
latus twice as long as broad; inframedian latus rectangular; rostral latus nearly rectangular
in outline; rostrum large, elongate triangular, broad above, pointed below. Cirrus I unequal,
anterior ramus oval, posterior ramus more slender; cirrus VI with caudal appendages. Maxillule
not notched stout spine along the cutting edge; mandible with three teeth excluding inferior
angle. Measurements of specimen: height of capitulum 9.67 mm, width 5.52 mm, thickness
2.58 mm; length of peduncle 2.79 mm, width 2.29 mm.
Distribution:
East China Sea, Pacific Ocean, Taiwan (Chan et al. 2009b). In this study, Catherinum rossi was
found at Tanimbar Island, Indonesia.
Type locality:
Stn. 3, Hess Guyot (174°24.8’ W, 17°53.2’ N), 1,692–1,735 m depth (Sigsbee beam trawl);
Stn. 1, Allison Guyot (179°36.0’ W, 18°31.0’ N), 1,413–1,645 m depth (otter trawl) (Rao and
Newman 1972).
Figure 3.22. Catherinum rossi (Rao & Newman, 1972) (MNHN-IU-2019-4933). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
rostral view showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 3 mm.
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115
Genus Planoscalpellum Zevina, 1978
Arcoscalpellum Hoek, 1907: 85 (part).
Planoscalpellum Zevina, 1978b: 1347.
Diagnosis:
Upper latus with umbo on scutal margin; inframedian latus with umbo apical or sub-basal;
carinal latus with umbo on carinal part, may be protruding.
Type species:
Scalpellum planum Hoek, 1883; type locality: 42°42’S, 134°10’E; depth: 4,755 m; bottom
temperature: 0.2°C; bottom: red clay.
Planoscalpellum distinctum (Hoek, 1883)
Figure 3.23
Scalpellum distinctum Hoek, 1883: 111–112, pl. VI, figs 10–11. — 1907: 83, pl. VII, fig. 12 — NilssonCantell 1927: 750, text-fig. 4.
Planoscalpellum distinctum. — Zevina 1981: 186–187. — Foster & Buckeridge 1995 a: 360–361, fig.
7(A–C) — Shalaeva & Boxshall 2014: 36, fig. 22.
Material examined:
Tanimbar Island: 2 specimens, MNHN-IU-2019-4928, Stn. DW 49, 08°00’S, 132°59’E,
206-210 m depth, 29 October 1991.
Diagnosis:
Capitulum with 13 plates separated from each other by distinct chitinous interspaces; scutum
almost triangular; upper latus hexagonal, large.
Description:
Capitulum flattened, elongate-oval shape; plates with distinct growth lines, separated from
each other by rather broad chitinous interspaces. Scutum triangular, occludent margin
arched, umbo of scutum apical, apex slightly turned upwards. Tergum triangular, occludent
margin not very long. Carina simply bowed. Upper latus irregularly hexagonal, rostral latus
irregularly quadrangular, infra-median latus in the form of wine-glass, carinal latus irregularly
quadrangular. Peduncle short, with very small, numerous, calcareous scales. Cirrus I unequal,
anterior ramus oval, posterior ramus more slender; cirrus VI with short caudal appendages.
Maxillule notched, three stout spines on the upper side; mandible with three teeth, excluding
inferior angle. Measurements of two specimens: height of capitulum 6.80–7.90 mm, width
3.68–4.36 mm, thickness 1.24–1.26 mm; length of peduncle 1.39–2.49 mm, width 1.80–2.04
mm.
Distribution:
Pacific, Western Central; known depth range 1,302 to 2,745 m (Shalaeva and Boxshall 2014). In
this study, Planoscalpellum distinctum was found at Tanimbar Island, Indonesia.
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Chapter 3
Type locality:
2°33’S, 144°4’E; depth: 1,926 m; bottom temperature: 2.1°C; bottom: Globigerina ooze (Hoek
1883).
Figure 3.23. Planoscalpellum distinctum (Hoek, 1883) (MNHN-IU-2019-4928). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
rostral view showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 2 mm.
Genus Teloscalpellum Zevina, 1978
Arcoscalpellum Hoek, 1907: 85 (sectio, part.). — Newman & Ross 1971: 42 (part.); Section V. — Pilsbry,
1907: 47 (part.).
Teloscalpellum Zevina, 1978b: 1350. — Liu & Ren 1985: 212. — 2007: 257.
Diagnosis:
Capitulum with 13 or 14 plates; carinal lateral umbo at basi-carinal angle, angle not extending
beyond carinal margin; inframedian latus triangular or rod-like-shaped, umbo apical or subapical; caudal appendage multi-segmented.
Type species:
Scalpellum spicatum Zevina, 1975.
Teloscalpellum ecaudatum (Calman, 1918)
Figure 3.24
Scalpellum ecaudatum Calman, 1918b: 106, text-fig. 2.
Teloscalpellum ecaudatum. — Zevina, 1981: 365, fig. 282. — Chan 2009: 55, figs 1E, 7.
Material examined:
Kei Islands: 1 specimen, MNHN-IU-2019-4917, Stn. CP 35, 06°08’S, 132°45’E, 390–502 m
depth, 27 October 1991.
Tanimbar Island: 1 specimen, MNHN-IU-2019-4918, Stn. CP 59, 08°20’S, 132°11’E, 399405 m depth, 31 October 1991.
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117
Diagnosis:
Capitulum compressed, elongated; 14 plates, sculptured with radiating ribs; inframedian latus
triangular; rostro-lateral plates very low; penis and caudal appendages absent.
Description:
Capitulum with all plates strongly ribbed, lines of growth marked by fine, inconspicuous
striation. Tergum with occludent margin straight, carinal margin convex, but straight concave
near apex. Scutum with occludent margin convex, other margins straight, apex slightly
overlapping tergum. Carina evenly curved with flat roof bordered by strong ridges; upper latus
quadrangular; rostrum small, triangular, overlapped at sides by rostral latera; inframedian
latus very narrow. Peduncle shorter than capitulum, covered with strong scales. Cirrus I
with unequal rami (anterior ramus: 11 segmented, posterior ramus: 13 segmented). Maxilla
globular with serrulate setae; maxillule not notched; mandible with three major teeth, lower
margin with three to four denticles. Measurements of two specimens: height of capitulum
12.07–18.59 mm, width 6.17–11.47 mm, thickness 2.68–5.41 mm; length of peduncle 6.30–
8.33 mm, width 3.21–5.41 mm.
Distribution:
Java Sea, Indonesia; Philippines (Chan 2009). In this study, Teloscalpellum ecaudatum was
found at Kei Islands and Tanimbar Island, Indonesia.
Type locality:
Java Sea; 7°35’S, 114°30’30” E; 132–315 m depth (Calman 1918b).
Figure 3.24. Teloscalpellum ecaudatum (Calman, 1918) (MNHN-IU-2019-4917). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
carinal view, (d) rostral view showing the capitulum and peduncle. Scale bars: a–d) 5 mm.
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Chapter 3
Genus Trianguloscalpellum Zevina, 1978b
Arcoscalpellum Hoek, 1907: 85 (part). — Pilsbry 1907a: 47. — Newman & Ross 1971: 42 (part).
Trianguloscalpellum Zevina, 1978b: 1349. — Liu & Ren 1985: 205.
Diagnosis:
Capitulum of female or hermaphrodite with 13 or 14 completely calcified plates; tergum and
scutum with umbones apical; middle latera triangular, sometimes quadrangular, with apical
umbo reaching upper latera; carinolatera triangular or subtriangular with apical umbo; caudal
appendages distinctly long.
Type species:
Scalpellum balanoides Hoek, 1883: 129, pl. V, fig. 15, pl. X, fig. 11, pl. XI, figs 1–3; type locality:
5°42’S, 132°25’E; depth: 236 m; bottom: mud; it was found attached to an arm of a Comatula
or Pentacrinus.
Trianguloscalpellum balanoides (Hoek, 1883)
Figure 3.25
Scalpellum balanoides Hoek,1883: 129, pl. V, fig. 15, pl. X, fig. 11, pl. XI, figs 1–3. — Broch 1922: 242,
fig. 10 — Weltner 1922: 63, taf. II, fig. 7, taf. III, fig. 8. — Nilsson-Cantell 1931: 2. — Hiro 1937b: 42,
fig. 33.
Scalpellum gonionotum Pilsbry, 1907b: 360. — Pilsbry 1911: 65, pl. IX, figs 2–4. — Hiro 1937a: 43.
Trianguloscalpellum balanoides. — Zevina 1978b: 1349. — 1981: 294, fig. 221. — Liu & Ren 1985: 210,
fig. 16, pl. XI, figs 15–17. — 2007: 256–257, fig. 111. — Rosell 1991: 20–22, fig. 2c–d. — Huang
1994: 517. — 2001: 318. — Jones et al. 2001: 253. —Young 2001: 464, fig. 7. — Jones 2007: 292.
— Chan et al. 2009b: 116, figs 95–96. — Shalaeva & Boxshall 2014: 40, fig. 26.
Material examined:
Kei Islands: 1 specimen, MNHN-IU-2019-4923, Stn. CP 16, 05°17’S, 132°50’E, 330–350 m
depth, 24 October 1991, attached to crinoid.
Tanimbar Island: 2 specimens, MNHN-IU-2019-4924, Stn. CP 86, 09°26’S, 131°13’E, 223–
225 m depth, 4 November 1991.
Diagnosis:
Capitulum with 13 fully calcified plates, covered by thin membrane; carina and extremely
short, simply bowed without distinct roof; umbo of carina at apex; upper latus triangular;
carinal latus almost as high as carina, umbo apical, carinal latus very large, almost similar size
to tergum.
Description:
Capitulum flat, narrow at base, same breadth as peduncle. Scutum about twice as long as
broad; occludent margin almost straight, umbo at apex. Tergum triangular, the same size
as scutum. Carina very short, simply, not very strongly, bowed, umbo at apex. Upper latus
triangular, carinal margin absent. Rostral latus quadrangular, umbo at apex of angle formed
Deep-sea barnacles from the Karubar expedition (1991)
119
by rostral and scutal margins. Infra-median latus elongate, triangular, umbo apical. Carinal
latus very large, convex, umbo at top of carinal margin. Cirrus I unequal, posterior ramus
more slender than anterior ramus; cirrus VI with caudal appendages. Maxillule distinctly
notched, two stout spines on upper side; mandible with three teeth excluding inferior angle.
Measurements of specimen: height of capitulum 8.20 mm, width 4.40 mm, thickness 1.75
mm; length of peduncle 5.06 mm, width 2.10 mm.
Distribution:
Pacific, Western Central; South and East China Sea; South of Sumatra, Banda Sea, Indonesia;
Vietnam; Philippines; Taiwan; South of Japan; attached to crinoids, hydroids; 220–1,097
m depth (Jones et al. 2001; Chan et al. 2009b; Shalaeva and Boxshall 2014). In this study,
Trianguloscalpellum balanoides was found at Kei Islands and Tanimbar Island, Indonesia.
Type locality:
5°42’S, 132°25’E; depth: 232 m; bottom: mud; attached to arm of a Comatula or Pentacrinus
(Hoek 1883).
Figure 3.25. Trianguloscalpellum balanoides (Hoek, 1883) (MNHN-IU-2019-4923). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
rostral view showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 2 mm.
Trianguloscalpellum hirsutum (Hoek, 1883)
Figure 3.26
Scalpellum hirsutum Hoek, 1883: 88, pl. IV, fig. 19. — Gruvel 1905: 66, fig. 74. — Pilsbry 1907a: 25.
Arcoscalpellum hirsutum. — Newman & Ross 1971: 62–64, fig. 28.
Trianguloscalpellum hirsutum. — Zevina 1981: 309, fig. 233. — Chan et al. 2009b: 119–121, figs 100–
102. — Chan et al. 2010: 13, figs 1F, 9A–H. — Jones 2012: 371, 376. — Shalaeva & Boxshall 2014:
44, fig. 29.
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Chapter 3
Material examined:
Tanimbar Island: 1 specimen, MNHN-IU-2019-4908, Stn. CP 91, 08°44’S, 131°05’E, 884–
891 m depth, 5 November 1991.
Diagnosis:
Capitulum with 14 plates covered by membrane, covered by very long hairs; carina simply
bowed, umbo at apex, roof flat; upper latus triangular; rostrum very narrow stripe distinctly
visible at surface.
Description:
Capitulum small, long hairs covering plates. Scutum elongate, convex, more than twice as
long as broad. Tergum large, elongate rhomboid, umbo at apex. Carina simply bowed, roof
flat. Upper latus almost triangular, carinal-basal margin arched. Rostrum small, linear-shaped.
Rostral latus quadrangular, basal and scutal margins parallel. Infra-median latus triangular,
umbo apical. Carinal latus with irregular shape, umbo near apex. Peduncle short. Cirrus I
with rami unequal, anterior ramus oval, posterior ramus more slender; cirrus VI with caudal
appendages. Maxillule not notched, two stout spines on upper side; mandible with three teeth
excluding inferior angle. Measurements of the specimen: height of capitulum 12.18 mm, width
6.50 mm, thickness 3.74 mm; length of peduncle 4.10 mm, width 3.43 mm.
Distribution:
Borneo, Indonesia; Pacific, Western Central and Southeast; Australia; Taiwan; Antarctica; attached
to gastropod shells, rocks; 1,502–1,965 m depth (Chan et al. 2010; Jones 2012; Shalaeva and Boxshall
2014). In this study, Trianguloscalpellum hirsutum was found at Tanimbar Island, Indonesia.
Type locality:
Pacific, Western Central (Moluccas Sea); 0°48›S, 120°58›E; depth: 252 m; bottom temperature:
2.4°C; bottom: rock (Hoek 1883).
Figure 3.26. Trianguloscalpellum hirsutum (Hoek, 1883) (MNHN-IU-2019-4908). (a) left lateral view
showing the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c)
carinal view, (d) rostral view showing the capitulum and peduncle. Scale bars: a–d) 5 mm.
Deep-sea barnacles from the Karubar expedition (1991)
121
Genus Verum Zevina, 1978
Arcoscalpellum Pilsbry, 1907: 47 (part). — Newman & Ross 1971: 42 (part).
Verum Zevina, 1978b: 1348.
Diagnosis:
Capitulum of female or hermaphrodite, with 13–14 completely calcified plates; tergum with
carinal margin straight or slightly to moderately recurving; scutum with or without short apicolateral arm, umbo apical; carina with apical or subapical umbo, evenly curved or angularly
flexed; upper latera sub-triangular or pentagonal, umbo apical or subapical; middle latera
narrow, triangular or quadrangular, umbo basal or sub-basal; carinolatera short, umbo basal to
sub-basocarinal, usually not or slightly projecting beyond rim of capitulum; caudal appendages
short.
Type species:
Scalpellum zenkevichi Zevina, 1972: 44, fig. 3; type locality: Pacific Ocean, 24°27’S, 70°42’E.
Verum australicum (Hoek, 1883)
Figure 3.27
Scalpellum australicum Hoek, 1883: 118, pl. V, fig. 11. — Weltner 1922: 63. — Nilsson-Cantell 1938: 7.
Verum australicum. — Zevina 1981: 223–224, fig. 160. — Jones et al. 1990: 5. — Jones 1992: 156–160,
figs 7–8. — Jones 2012: 371, 376. — Shalaeva & Boxshall 2014: 52, fig. 36.
Material examined:
Tanimbar Island: 1 specimen, MNHN-IU-2019-4930, Stn. CP 86, 09°26’S, 131°13’E, 223–
225 m depth, 4 November 1991.
Diagnosis:
Capitulum with 13 plates closely locked together, surface smooth with slightly prominent
ridges; carina simply bowed with flat roof; upper latus trapeziform; inframedian latus elongate,
narrow; carinal latus with umbo projecting beyond carina.
Description:
Capitulum elongate-oval, flat with distinct ridges and furrow. Scutum slightly convex, umbo
apical, occludent margin arched. Tergum triangular, umbo apical, distinctly recurved. Rostral
latus irregularly quadrangular; infra median latus narrow, umbo near base; carinal latus
quadrangular. Peduncle slightly conical, short, scales near capitulum larger than those near
base. Cirrus I unequal, anterior ramus oval, posterior ramus more slender; cirri II to VI very
long, rami subequal, segments elongate; cirrus VI with caudal appendages. Maxillule not
notched, large spine on upper side, cutting edge almost straight; mandible with three large
teeth excluding inferior angle. Measurements of specimen: height of capitulum 10.33 mm,
width 6.09 mm, thickness 2.08 mm; length of peduncle 4.62 mm, width 2.24 mm.
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Chapter 3
Distribution:
Indian Ocean, Western; Pacific Western Central and Southeast; Zanzibar; known depth range
463–2,561 m (Shalaeva and Boxshall 2014). In this study, Verum australicum was found at
Tanimbar Island, Indonesia.
Type locality:
Between New Guinea and Australia (12°08’S, 145°10’E): Challenger station 184: depth: 2,561
m: bottom temperature: 1.8°C; substrate grey ooze (Hoek 1883).
Figure 3.27. Verum australicum (Hoek, 1883) (MNHN-IU-2019-4930). (a) left lateral view showing the
capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c) rostral view
showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 3 mm.
Verum carinatum (Hoek, 1883)
Figure 3.28
Scalpellum carinatum Hoek, 1883: 76, pl. III, figs 7–8. — Pilsbry 1907a: 53, fig. 18. — Gruvel 1920: 20.
— Barnard 1925: 3. — Broch 1953: 7. — Weisbord 1977: 243–244, pl. 27, figs 3–4.
Scalpellum imperfectum Pilsbry, 1907a: 75, fig. 30, pl. IV, figs 15–18. — Annandale 1913: 233. — Barnard
1924: 47. — MacDonald 1929: 537. — Broch, 1953: 9. — Stubbings 1961: 11, text-fig. 2. — 1967:
234.
Meroscalpellum imperfectum. — Newman & Ross 1971: 119, text-fig. 62.
Verum carinatum. — Zevina 1981: 225–226, fig. 163. — Shalaeva & Boxshall 2014: 53, fig. 37.
Material examined:
Kei Islands: 1 specimen, MNHN-IU-2019-4920, Stn. DW 18, 05°18’S, 133°01’E, 205–212
m depth, 24 October 1991.
Deep-sea barnacles from the Karubar expedition (1991)
123
Diagnosis:
Capitulum with 14 plates separated by chitinous interspaces, surface smooth; carina bowed,
angularly bent, with flat roof; upper latus irregular pentagonal; inframedian latus large, wineglass-shaped.
Description:
Capitulum elongate, plates covered by thin, chitinous membrane. Scutum elongated, apex
pointed, occludent margin very convex. Tergum flat, triangular, apex very recurved, occludent
margin very arched. Carina with umbo at top of flat roof. Upper latus flat, irregular pentagonal;
rostrum narrow, elongated; rostral lateral convex with rostral margin short; inframedian latus
wine-glass-shaped; carinal latus flat, large. Peduncle short, calcareous scales distinct. Cirrus
I unequal, anterior ramus broader than posterior ramus; cirri II to VI long, rami equal; cirrus
VI with caudal appendages. Maxillule not notched, two large spines on upper side, cutting
edge almost straight; mandible with three large teeth excluding inferior angle. Measurements
of specimen: height of capitulum 9.14 mm, width 4.53 mm, thickness 1.74 mm; length of
peduncle 2.54 mm and width 2.20 mm.
Distribution:
Atlantic, excluding polar areas; Pacific, Southeast. Known depth range 600 to 2,400 m (Shalaeva
and Boxshall 2014). In this study, Verum carinatum was found at Kei Islands, Indonesia.
Type locality:
Station 135, near the Island of Tristan da Cunha; depth: 1,800 m; bottom: rock, shells (Hoek
1883).
Figure 3.28. Verum carinatum (Hoek, 1883) (MNHN-IU-2019-4920). (a) left lateral view showing the
capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c) rostral view
showing the capitulum and peduncle, (d) carinal view. Scale bars: a–d) 3 mm.
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Chapter 3
Verum novaezelandiae (Hoek, 1883)
Figure 3.29
Scalpellum novae-zelandiae Hoek, 1883: 124, pl. V, figs 7, 8. — 1907: 100, pl. VIII, figs 4, 4a; 1913: 14.
— Weltner 1897: 249. — 1922: 71. — Annandale 1905: 83. — 1906b: 389. — 1908: pl. 5, fig. 7. —
1913: 231. — Broch 1927a: 540, fig. 512.
Scalpellum Novae-Zealandiae. — Gruvel 1905: 88, fig. 99.
Scalpellum (Scalpellum) novae-zelandiae. — Calman 1918b: 123.
Arcoscalpellum novaezelandiae. — Foster 1978: 65, pl. 8D, fig. 39.
non Scalpellum Novae-Zelandiae. — Gruvel 1902: 54, pl. 2, figs 12–13, 15. — Gruvel 1912: 346 (= V.
parazelandiae Young, 1998b).
Verum novaezelandiae. — Zevina 1978b: 1348. — Zevina 1981: 228, fig. 165. — Chan et al. 2009b: 17,
figs 1I, 12A–H, 13A–D. — Chan et al. 2010: 17, figs 11–13. — Shalaeva & Boxshall 2014: 53, fig. 39.
Material examined:
Tanimbar Island: 1 specimen, MNHN-IU-2019-4919, Stn. CP 87, 08°47’S, 130°49’E, 1017–
1024 m depth, 5 November 1991.
Diagnosis:
Capitulum with 13 plates covered by very thin, chitinous membrane; carina simply bowed,
flat roof with umbo apical; upper latus quadrangular or rather pentagonal, lower edge being
truncated; inframedian latus elongate, quadrangular; carinal latus large, elongate, umbo near
base; peduncle short.
Description:
Capitulum flatted, elongate, plates separated by narrow, chitinous interspaces. Scutum
quadrangular, umbo apical, occludent margin arched. Tergum triangular with umbo slightly
produced apex. Carina with bowed, flat roof, increasing little in width from upper to lower. Upper
latus irregularly trapeziform; rostral latus with umbo at top of rostral margin; infra-median latus
irregular, quadrangular, elongate, narrow; carina latus quadrangular. Cirrus I unequal, anterior
ramus broader than posterior ramus; cirrus VI with short caudal appendages. Maxillule slightly
notched, two large spines on upper side; mandible with three large teeth excluding inferior
angle; labrum slightly convex, with numerous teeth. Measurements of specimen: height of
capitulum 13.46 mm, width 6.98 mm, thickness 2.99 mm; length of peduncle 6.02 mm, width
3.23 mm.
Distribution:
Indo-west Pacific: Eastern Africa, Gulf of Aden, eastwards to New Zealand; Philippines; Taiwan;
attached to sunken wood, gorgonians, glassy spicule of hexactinellid sponges; 822–4850 m
depth (Shalaeva and Boxshall 2014; Jones and Hosie 2016). In this study, Verum novaezelandiae
was found at Tanimbar Island, Indonesia.
Deep-sea barnacles from the Karubar expedition (1991)
125
Type locality:
37°34’S, 179°22’E; depth: 1,260 m; bottom temperature: 4.2°C; bottom: grey ooze (Hoek
1883).
Figure 3.29. Verum novaezelandiae (Hoek, 1883) (MNHN-IU-2019-4919). (a) left lateral view showing
the capitulum and peduncle, (b) right lateral view showing the capitulum and peduncle, (c) carinal
view, (d) rostral view showing the capitulum and peduncle. Scale bars: a–d) 3 mm.
Order Sessilia Lamarck, 1818
Suborder Verrucomorpha, Pilsbry 1916
Verrucomorpha Pilsbry, 1916: 14. — Newman et al. 1969: 281. — Newman & Ross 1971: 135. —
Newman 1987: 8. — 1996: 501. — Buckeridge 1994: 89. — Young, 1998: 74.
Diagnosis:
Shell asymmetrical with four plates (carina, rostrum, tergum and scutum), base membranous
or calcareous.
Family Verrucidae Darwin, 1854
Verrucidae Darwin, 1854: 495. — Gruvel 1905: 169. — Withers 1935: 323. — Newman et al.
1969: 281. — Newman & Ross 1971: 135. — Buckeridge 1994: 89. — Young 1998: 74.
Diagnosis:
Scutum and tergum without depressor muscles; movable only on one side; other side
immovable, united with rostrum and carina.
Genus Alteriverruca Pilsbry, 1916
Verruca Section D Altiverruca Pilsbry, 1916: 40.
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Chapter 3
Altiverruca Pilsbry, 1916: 40 — Newman et al. 1969: 282. — Newman & Ross 1971: 135. — Ren 1984b:
172. — Buckeridge 1994: 92. — Young 1998: 77.
Diagnosis:
Verrucids with erect form; bases of plates not inflected. Operculum close to vertical, myophore
absent.
Type species:
Verruca hoeki Pilsbry, 1907.
Altiverruca navicula (Hoek, 1913)
Figure 3.30
Verruca navicula Hoek, 1913: 134, figs 4–6. — Nilsson-Cantell 1927: 778, figs a–f.
Altiverruca navicula. — Buckeridge 1994: 100, fig. 5. — Chan et al. 2009b: 280, figs 245–247. — Chan
et al. 2010: 29, figs 2E, 21, 22.
Material examined:
Tanimbar Island: 3 specimens, MNHN-IU-2019-4941, Stn. CP 91, 08°44’S, 131°05’E,
884–891 m depth, 5 November 1991, attached to peduncle of Alcockianum persona
(Annandale, 1916).
Diagnosis:
Shell not depressed; carina and rostrum interlocking with single rib from each plate; movable
plates large, scutum with four articular ribs, tergum with six articular ribs, growth lines very
distinct; caudal appendages long.
Description:
Shell yellowish. Movable scutum elongately triangular, apex distinctly beaked, projecting freely;
surface with numerous articular ridges. Movable tergum large, quadrangular; surface with
strongly developed, curved axial articular ridge. Carina and rostrum irregular quadrangular,
with carina higher, rostrum broader. Fixed tergum with two parts: (1) triangular portion very
narrow at apex, slightly broader in its inferior (2) flat and broad part at a rear portion of
shell. Fixed scutum pointed with distinctly beaked apex; composed of broader, nearly flat,
triangular portion and narrower inflected portion, only widening towards its inferior. Base of
shell elongatedly oval-shaped. Cirrus I with rami very unequal (anterior ramus: 12-segmented,
posterior ramus: 28-segmented); cirrus VI with caudal appendages. Maxilla bilobed, fringed
with setae, except on the notch; maxillule widely notched, horizontally elongated, two large
spines above notch, numerous dense setae at notch; mandible with three teeth excluding
inferior angle; labrum slightly concave, conical teeth on cutting margin.
Distribution:
Pacific Ocean (Chan et al. 2010). In this study, Altiverruca navicula was found at Tanimbar
Island, Indonesia.
Deep-sea barnacles from the Karubar expedition (1991)
127
Type locality:
(1) 9°3.4’S, 119°56.7’E; depth 959 m; bottom: globigerina ooze. (2) 3° 37.7’S, 131°26’.4E; depth
924 m; bottom: fine grey mud (Hoek 1913).
Figure 3.30. Altiverruca navicula (Hoek, 1913) (MNHN-IU-2019-4941). (a) left lateral view, (b) right
lateral view, (c) upper lateral view, (d) lower lateral view. Scale bars: a–d) 7 mm.
Genus Newmaniverruca Young, 1998
Verruca Section B: Verruca, Group of Verruca alba Pilsbry, 1916: 25 (part.).
Verruca Section B: Verruca, Group of Verruca calotheca Pilsbry, 1916: 30 (part.).
Verruca (Verruca): Foster, 1979: 68 (part.). — Zevina 1987: 1812 (part. — Buckeridge 1994: 90 (part.).
Newmaniverruca Young, 1998: 77.
Diagnosis:
Shell with four plates (carina, rostrum, scutum and tergum); operculum parallel to base.
Type species:
Verruca albatrossiana Pilsbry, 1912: 292. — 1916: 47; type locality: East of Luzon, Philippines.
Newmaniverruca albatrossiana (Pilsbry, 1912)
Figure 3.31
Verruca albatrossiana Pilsbry, 1912: 292. — 1916: 47. — Buckeridge, 1994: 91, figs 1a–f. — Buckeridge,
1997: 129.
Verruca grex Hoek, 1913: 142, pl. XI, figs 7–13, pl. XIII, figs 11–13.
Verruca (Euverruca) albatrossiana. — Broch 1931: 45.
Verruca (Verruca) albatrossiana. — Ren 1984: 168, fig. 2, pl. 1 (7–11).
Newmaniverruca albatrossiana. — Young 1998: 77. — Chan 2009: 74, figs 2G, 22A–I.
Material examined:
Kei Islands: 1 specimen, MNHN-IU-2019-4942, Stn. CC 21, 05°14’S, 133°00’E, 688–694 m
depth, 25 October 1991, attached to glass rope sponge.
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Chapter 3
Diagnosis:
Rostrum and fixed scutum with unusual length; rostrum low, fixed scutum larger than fixed
tergum.
Description:
Movable plates parallel to base, wall of parietal vertically ribbed; fixed scutum without internal
pit. Movable scutum with crescentic ridge and longitudinal striations; movable tergum with
articular ribs and diagonal rib. Apices of fixed scutum and tergum contiguous. Carina occupying
carino-rostral wall, apices marginal. Cirrus I with rami unequal and serrulate setae; cirrus VI
with caudal appendages. Maxilla globular, with fringing setae; maxillule notched, two large
setae on upper side; mandible with three teeth excluding inferior angle.
Distribution:
Indo-west Pacific: NW Australia; South China Sea; China; Philippines; attached to cidaroid
spines (e.g. Stereocidaris indica philippinensis Mortensen, 1928), 345–620 m depth (Jones and
Hosie 2016). In this study, Newmaniverruca albatrossiana was found at Kei Islands, Indonesia.
Type locality:
East of Luzon, Philippines (Pilsbry 1912).
Figure 3.31. Newmaniverruca albatrossiana (Pilsbry, 1912) (MNHN-IU-2019-4942). (a) left lateral view,
(b) right lateral view, (c) upper lateral view, (d) lower lateral view. Scale bars: a–d) 3 mm.
Suborder Balanomorpha Pilsbry, 1916
Operculata tribe Symetrica Gruvel, 1905: 189.
Balanomorpha Pilsbry, 1916: 47 (Balanidae sensu Darwin, 1854).
Diagnosis:
Peduncle absent; shells bilaterally symmetrical on either side of rostrocarinal axis; shell
with parietal plates of carina, rostrum and one to three pairs of lateral plates, all separate
from one another, variously fused or totally concrescent; base membranous or calcareous;
hermaphroditic or with dwarf males.
Deep-sea barnacles from the Karubar expedition (1991)
129
Family Bathylasmatidae Newman & Ross, 1971
Bathylasmatidae Newman & Ross, 1971: 138 (part.). — Newman & Ross 1976: 37, 45 (part.).
Bathylasmatinae Newman & Ross, 1976: 37, 45. — Buckeridge 1983: 68. — Newman 1996: 502. —
Buckeridge 1999: 522. — Jones 2000: 231, tables 1–2.
Diagnosis:
Shell with four or six plates; wall solid or permeated by single row of chitin-filled longitudinal
canals; radii absent; one or both rami of cirri I and cirri II sometimes antenniform; labrum
without notch in crest.
Subfamily Hexelasminae Newman & Ross, 1976
Hexelasminae Newman & Ross, 1976: 37, 46.
Diagnosis:
Shell with six plates, infiltrated by chitin-filled tubes; basis calcareous; scuta parallel to basis;
tergum with distinct spur; cirrus II resembling cirrus I more than cirrus III.
Type genus:
Hexalasma Hoek, 1913.
Genus Hexelasma Hoek, 1913
Hexelasma Hoek, 1913: 244 (part.). — Utinomi 1965: 13. — Jones 2000: 240, figs 51, 64, tables 28–30.
Aaptolasma Newman & Ross, 1971: 158.
Diagnosis:
Parietes solid; radii absent; plates with a carina, paired carinolatera and latera and a rostrum
without any sign of fusion; caudal appendages absent.
Type species:
Hexelasma velutinum Hoek, 1913: 246 (part.); type locality: Indonesia, 6°08’S, 121°19’E (Siboga
Station 105).
Hexelasma arafurae Hoek, 1913
Figure 3.32
Hexelasma arafurae Hoek, 1913: 251, pl. 25, figs 12–16. — Utinomi 1965: 11. — Newman & Ross 1971:
155. — 1976: 46. — Foster 1981: figs 6 F–H. — Jones 2000: 246, fig. 51, tables 28–31.
Aaptolasma arafura. — Foster 1978: 79.
Material examined:
Kei Islands: 2 specimens, MNHN-IU-2019-4851, Stn. DW 18, 05°18’S, 133°01’E, 205–212
m depth, 24 October 1991.
Diagnosis:
Shell with very wide orifice; alae broadly-triangular with summits oblique; scutum folded
longitudinally, articular ridge slightly prominent, deep pit for adductor muscle; tergum beaked,
with prominent articular ridge, spur very broadly rounded; inner side of scutum and tergum
orange-coloured.
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Chapter 3
Description:
Shell yellowish, conical, with six plates. Orifice diamond-shaped; scutum triangular elongated
with protruding growth-ridges; tergum smaller than scutum, apex beaked, carinal margin
rounded, growth-ridges less distinct than on scutum. Cirrus I with unequal rami (anterior
ramus: 8-segmented; posterior ramus: 12-segmented), dense long setae on surface areas.
Cirrus II with equal rami, dense long setae. Cirri IV–VI with equal rami with numerous segments;
segments almost without exception furnished with two pairs very long, stiff, needle-like spines
along inner faces. Measurements of specimen: basal length of shell 14.32 mm, orifice length
8.00 mm, carinal height 12.39 mm, orifice width 6.49 mm, basal width 13.76 mm.
Distribution:
Arafura Sea, Indonesia; 205–560 m depth (Jones and Hosie 2016). In this study, Hexelasma
arafurae was found at Kei Islands, Indonesia.
Type locality:
Indonesia; Siboga stat. 262; 5°54’S, 132°49’E; 560 m depth; bottom: solid bluish-grey mud
(Hoek 1913).
Figure 3.32. Hexelasma arafurae Hoek, 1913 (MNHN-IU-2019-4851). (a) view from above, (b) right
lateral view. Scale bars: a, b) 4 mm.
Superfamily Balanoidea Leach, 1817
Balanidae Leach, 1817: 68 — Pilsbry 1916: 48 — Newman et al. 1969: 284 — Newman & Ross 1976:
38, 49 — Newman 1993: 408.
Balaninae — Darwin 1854: 175.
Balanoidea — Newman 1996: 502.
Diagnosis:
Shell wall composed of four or six plates (rostrum, carina and one to three pairs of laterals);
parietes solid or tubiferous, when tubiferous rarely secondarily filled; radii solid or tubiferous.
Deep-sea barnacles from the Karubar expedition (1991)
131
Basis commonly calcareous, solid or permeated by tubes, rarely membranous; when basis
calcareous, internal surfaces of compartments commonly with uniform ribs; when calcareous
commonly forming complex interdigitations with wall. Opercular plates occlude aperture;
articulations between the pairs generally shallow or fused. Cirrus I with rami subequal or
grossly unequal; cirri II and III with rami never antenniform; cirrus III resembling II more than
IV; caudal appendages absent; penis with basidorsal point. Labrum thin, never bullate; crest
with pronounced medial incision; mandible quadri- or quinquedentoid; second and following
teeth with one or more subsidiary cusps; fifth tooth often vestigial; inferior angle commonly
molariform.
Family Archaeobalanidae Newman & Ross, 1976
Archaeobalanidae Newman & Ross, 1976: 38, 49.
Diagnosis:
Shell with four or sixplates; parietes solid, rarely tubiferous; tubes uniformly or irregularly
arranged; radii solid; basis commonly calcareous, rarely tubiferous.
Subfamily Archaeobalaninae Newman & Ross, 1976
Archaeobalaninae Newman & Ross, 1976: 38, 49.
Diagnosis:
Shell with four or six plates; parietes solid or tubiferous; when tubiferous, tubes uniformly
arranged in single row; interlaminate figures simple; basis calcareous or membranous, when
membranous wall solid.
Type genus:
Archaeobalanus Menesini, 1971: 19.
Genus Conopea Say, 1822
Conopea Say, 1822: 323.
Conoplea Gray, 1825: 98, 103.
Balaninus Costa, 1839: 181.
Balanus Section B Darwin, 1854: 216.
Patella-Balanus Hoek, 1913: 160, 162, 221.
Diagnosis:
Shell firm, strong, with six thick compartments; parietes with or without pores; radii solid with
summits parallel to basal margin of parietes and denticulated sutural margins; basis calcareous,
elongated along carino-rostral axis, boat-shaped; orifice smooth, not dentated; scutum with
simple growth ridges; penis with basidorsal point; attached to gorgonians or antipatharians.
Type species:
Conopea elongata Say, 1822; type locality: eastern Florida, USA.
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Chapter 3
Conopea cymbiformis (Darwin, 1854)
Figure 3.33
Balanus cymbiformis Darwin, 1854: 221, pl. 3, figs 5a, b. — Broch 1931: 85, fig. 29a–b. — NilssonCantell 1938: 55, pl. 2, fig. 3. — Utinomi 1962: 219, fig. 2. — Dong et al. 1982: 103.
Balanus proripiens Hoek, 1913: 228, pl. 24, figs 1–3, pl. 24, figs 1–3. — Nilsson-Cantell 1921: 331, fig.
70 c–d.
Pyrgoma jedani Hoek, 1913: 262, pl. 27, figs 3–8.
Conopea cymbiformis. — Newman & Ross 1976: 55.
Material examined:
Kei Islands: 9 specimens, MNHN-IU-2019-4823, Stn. DW 30, 05°39’S, 132°56’E, 111–118
m depth, 26 October 1991; 1 specimen, MNHN-IU-2019-4824, Stn. DW 22, 05°22’S,
133°01’E, 82 m depth, 25 October 1991.
Diagnosis:
Shell irregularly conical; orifice oval with swollen border; basis irregularly cup-formed; scutum
and tergum triangular, not calcified together; spur of tergum feebly developed.
Description:
Shell yellowish with orange rust-brown in proximal areas. Carina, carinolatera and latera with
pale orange-brown and rust red-brown longitudinal stripes, latter may have oblique white
spots. Radii with pale orange-brown and rust red-brown horizontal striation. Opercular plates
with scutum pink-brown, transparent; tergum transparent white. Shell may appear longer
and lower, due to elongation of carina and rostrum or low and comparatively shorter, due to
development of rostrum alone or more upright and comparatively higher, with neither carina
nor rostrum elongated. Cirrus I with unequal rami (anterior ramus: 7-segmented; posterior
ramus: 12-segmented). Cirri II–VI with equal rami, numerous segments. Penis very long,
delicate hairs scattered over surface, a few more disposed near tip. Labrum deeply notched,
two small teeth on each side of notch. Mandibles with five teeth, inferior angle not distinctly
separated from fifth; distance between tips of first and second teeth slightly more than that
between those of second and third teeth; third tooth larger; fourth and fifth smaller than
others. Maxillule with straight edge and numerous large setae. Measurements of specimen:
basal length of shell 7.73 mm, orifice length 4.60 mm, carinal height 8.70 mm, orifice width
3.88 mm, basal width 4.94 mm.
Distribution:
Indo-west Pacific: Indian Ocean; Gulf of Aden, India, east to Fiji and NW to Indonesia, N
Australia, Malay Arch.; China; Philippines; S Japan; Fiji Is; attached to coenosarc of gorgonians
or antipatharians; littoral–453 m depth (Jones and Hosie 2016). In this study, Conopea
cymbiformis was found at Kei Islands, Indonesia.
Deep-sea barnacles from the Karubar expedition (1991)
133
Type locality:
Near Madras, India; attached to a gorgonian (Darwin 1854).
Figure 3.33. Conopea cymbiformis (Darwin, 1854) (MNHN-IU-2019-4823). (a) left lateral view, (b) right
lateral view, (c) view from above, (d) basal view. Scale bars: a–d) 3 mm.
Conopea navicula (Darwin, 1854)
Figure 3.34
Balanus navicula Darwin, 1854: 221, pl. 3, fig. 6a–d. — Hoek 1913: 223, pl. 22, fig. 26, pl. 23, figs 1–3.
— Stubbings 1936: 48. — Utinomi 1962: 74, fig 1. — Dong et al. 1982: 103.
Acasta spinitergum Foster, 1982: 209, fig. 4d.
Conopea navicula. — Newman & Ross 1976: 55.
Material examined:
Tanimbar Island: 12 specimens, MNHN-IU-2019-4821, Stn. CP 82, 09°32’S, 131°02’E,
215–219 m depth, 4 November 1991 attached to gorgonian; 4 specimens, MNHNIU-2019-4822, Stn. DW 49, 08°00’S, 132°59’E, 206–210 m depth, 29 October 1991,
attached to gorgonian.
Diagnosis:
Shell with parietes and basis not porose; carino-lateral compartments very narrow, almost
same width from top to bottom; radii with smooth sutural edges; scutum externally striated
longitudinally.
Description:
Specimens covered with coenosarc of coral, except orifice. Easily recognisable species due to
narrow carino-lateral plate, which is nearly same width at top as bottom; scutum externally
longitudinally striated; parietal plates studded with calcareous points. Parietal plates pearly
white, solid, superficially appearing to possess longitudinal tubes, growth lines horizontal.
Alae moderately developed. Basis calcareous. Size small. Rostrum well developed, concave,
lying at angle of ~ 45°. Laterals very well developed. Carino lateral parietes thin, radii and
134
Chapter 3
alae well developed. Carina tall, about half width of rostrum. External surfaces of all parietes
with very small, calcareous studs, regularly spaced, arranged along horizontal growth
lines. Opercular plates sunk down into orifice. Cirrus I with unequal rami (anterior ramus:
5-segmented; posterior ramus: 7-segmented). Cirrus II with unequal rami (anterior ramus:
6-segmented; posterior ramus: 9-segmented). Cirri III–VI with subequal rami more slender,
longer, with segments more elongate. Penis very long, tapering towards tip, bearing few, very
minute hairs. Maxilulle with straight edge with numerous large setae. Mandibles with five
teeth and inferior angle. Measurements of four specimens: basal length of shell 2.23–4.22
mm, orifice length 1.09–2.02 mm, carinal height 2.04–3.09 mm, orifice width 0.94–1.59 mm,
basal width 1.79–2.80 mm.
Distribution:
Indo-west Pacific, from Gulfs of Aden and Persia, India, Malaysia, Indonesia, Gulf of Siam, to
southern Japan; 45–220 m depth (Jones and Hosie 2016). In this study, Conopea navicula was
found at Tanimbar Island, Indonesia.
Type locality:
Madras, India; attached to gorgonian (Darwin 1854).
Figure 3.34. Conopea navicula (Darwin, 1854) (MNHN-IU-2019-4822). (a) left lateral view, (b) orifice
view. Scale bars: a, b) 1 mm.
Genus Solidobalanus Hoek, 1913
Solido–Balanus Hoek, 1913: 159, 192.
Balanus (Solidobalanus). — Pilsbry 1916: 220.
Solidobalanus. — Newman & Ross 1976: 23, 50.
Deep-sea barnacles from the Karubar expedition (1991)
135
Diagnosis:
Shell parietes solid, six plates; radii solid, well developed, with denticulate sutural edges; basis
calcareous, solid; complemental male, when present, in pit of rostral plate of hermaphrodite.
Type species:
Balanus auricoma Hoek, 1913: 198, pl. XVIII, figs 20–22, pl. XIX, figs 1–7; type locality: Ternate,
Indonesia (Siboga station 136).
Subgenus Solidobalanus Hoek, 1913
Solido–Balanus Hoek, 1913: 159, 192.
Balanus (Solidobalanus). — Pilsbry 1916: 220.
Solidobalanus. — Newman & Ross 1976: 23, 50.
Diagnosis:
Parietes and radii rather thick, smooth, without pores; basis flat, calcareous; rostrum not
very elongated; radii with strongly septate sutural edges; tergal spur narrow; crests for lateral
depressor muscles of scutum absent; adductor ridge weak or absent; tergum flat or with
shallow furrow without infolded sides; conspicuous teeth on anterior margins of fourth cirri
absent.
Type species:
Balanus auricoma Hoek, 1913: 198, pl. XVIII, figs 20–22, pl. XIX, figs 1–7; type locality: Ternate,
Indonesia (Siboga station 136).
Solidobalanus auricoma (Hoek, 1913)
Figure 3.35
Balanus (Solidobalanus) auricoma Hoek, 1913: 198, pl. XVIII, figs 20–22, pl. XIX, figs 1–7. — Broch 1922:
323, fig. 62. — 1931: 71. — Foster 1978: 100, fig. 60. — Rosell 1981: 303.
Solidobalanus (Solidobalanus) auricoma. — Newman & Ross 1976: 50.
Solidobalanus auricoma. — Foster 1981: 364, fig. 2G. — Rossel 1991: 38.
Material examined:
Tanimbar Island: 2 specimens, MNHN-IU-2019-4842, Stn. CP 45, 07°54’S, 132°47’E, 302–
305 m depth, 29 October 1991, shell only; 10 specimens, MNHN-IU-2019-4841, Stn. DW
49, 08°00’S, 132°59’E, 206–210 m depth, 29 October 1991, attached to sea urchin spines;
1 specimen, MNHN-IU-2019-4838, Stn. CP 82, 09°32’S, 131°02’E, 215–219 m depth, 4
November 1991, attached to antipatharian; 7 specimens, MNHN-IU-2019-4847, Stn. CP
85, 09°22’S, 131°14’E, 240–245 m depth, 4 November 1991, attached to spines of sea
urchin; 63 specimens, MNHN-IU-2019-4850, Stn. CP 86, 09°26’S, 131°13’E, 223–225 m
depth, 4 November 1991, attached to spines of sea-urchins.
Kei Islands: 1 specimen, MNHN-IU-2019-4849, Stn. CP 05, 05°49’S, 132°18’E, 296–299
m depth, 22 October 1991, attached to lateral plate of Chirona tenuis; 1 specimen,
MNHN-IU-2019-4844, Stn. CP 12, 05°23’S, 132°37’E, 413–436 m depth, 23 October
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Chapter 3
1991, attached to antipatharian; 6 specimens, MNHN-IU-2019-4848, Stn. CP 16, 05°17’S,
132°50’E, 330–350 m depth, 24 October 1991, attached to dead gorgonian, spines of
sea-urchins, arm of crinoid; 3 specimens, MNHN-IU-2019-4840, Stn. CP 35, 06°08’S,
132°45’E, 390–502 m depth, 27 October 1991; 1 specimen, MNHN-IU-2019-4843, Stn.
CP 36, 06°05’S, 132°44’E, 210–268 m depth, 27 October 1991.
Diagnosis:
Shell flatly-conical, colour reddish basally, whitish apically; radii narrow, summits slightly oblique;
opercular plates with golden hairs along occludent margins; scutum with articular ridge slightly
prominent, adductor ridge absent; tergum narrow, scutal margin distinctly dentated.
Description:
Shell with plates ribbed longitudinally. Shell colour brownish-pink to dull rose-pink, ribs tending
to white, colour often faded with specimens appearing uniform white. Parietes of carinolatera
very narrow, with single, conspicuous, longitudinal ridge. Scutum with occludent margin
straight, surface indistinctly ridged, pit for adductor muscle scarcely visible. Tergum short,
narrow, scutal margin straight, unusually distinctly dentated, carinal margin short, convex,
depressor muscle crests moderately well developed. Opercular plates with long, golden setae
fringing occludent margins, especially distally. Cirri I–II with rami slightly unequal, covered
with setae; cirri III–VI longer, more slender, dense setae on inner face. Mandible with four
teeth, second to fourth with accessory cusps, lower angle molariform with three blunt cusps
in series, lower edge with row of stiff setae. Measurements of five specimens: basal length of
shell 6.11–8.18 mm, orifice length 4.18–5.83 mm, carinal height 4.11–6.30 mm, orifice width
2.58–3.19 mm, basal width 4.51–5.96 mm.
Distribution:
Banda Sea (Moluccas, Indonesia); SW Australia; New Zealand; New Caledonia; Philippines to
southern Japan; Malaysian water; Gulf of Oman, Persia. 27–502 m depth (Jones and Hosie 2016).
In this study, Solidobalanus auricoma was found at Kei Islands and Tanimbar Island, Indonesia.
Figure 3.35. Solidobalanus auricoma (Hoek, 1913) (MNHN-IU-2019-4850). (a) upper lateral view from
above, (b) left lateral view, (c) right lateral view, (d) basal view. Scale bars: a–d) 2 mm.
Deep-sea barnacles from the Karubar expedition (1991)
137
Type locality:
Ternate anchorage; 27 m depth; bottom: mud and stone; numerous specimens on the surface
of pieces of rock (Hoek 1913).
Solidobalanus pseudauricoma (Broch, 1931)
Figure 3.36
Balanus (Solidobalanus) pseudauricoma Broch, 1931. — Utinomi 1949: 97, fig. 4.
Solidobalanus (Solidobalanus) pseudauricoma. — Newman & Ross 1976: 51. — Jones 2007: 294.
Material examined:
Kei Islands: 46 specimens, MNHN-IU-2019-4818, Stn. CP 25, 05°30’S, 132°52’E, 336–346
m depth, 26 October 1991, 30 specimens attached to gorgonian, 16 specimens attached
to coral; 6 specimens, MNHN-IU-2019-4825, Stn. CP 27, 05°33’S, 132°51’E, 304–314
m depth, 27 October 1991; 1 specimen, MNHN-IU-2019-4826, Stn. CP 05, 05°49’S,
132°18’E, 296–299 m depth, 22 October 1991, attached to crinoid; 13 specimens, MNHNIU-2019-4827, Stn. CP 16, 05°17’S, 132°50’E, 330–350 m depth, 24 October 1991; 2
specimens, MNHN-IU-2019-4828, Stn. DW 22, 05°22’S, 133°01’E, 82 m depth, 25 October
1991, 1 specimen attached to gorgonian stem; 8 specimens, MNHN-IU-2019-4830, Stn.
CP 05, 05°49’S, 132°18’E, 296–299 m depth, 22 October 1991, 5 specimens attached to
gorgonian, 1 specimen attached to crinoid.
Tanimbar Island: 11 specimens, MNHN-IU-2019-4839, Stn. CP 86, 09°26’S, 131°13’E,
223–225 m depth, 4 November 1991, several specimens attached to spines of sea urchins
(associated with Solidobalanus auricoma (Hoek, 1913)).
Diagnosis:
Shell with smooth, glossy white plates, coloured stripes absent; internal plates with thick,
solid, finely ribbed longitudinally; base non-porous, radially ribbed.
Description:
Shell plates white, stripes absent. Several specimens with pale pink tinge, one with pale
brownish-pink parietes with small, narrow ellipsoidal whitish spots, latter orientated
longitudinally producing reticulated effect. Radii whitish, pink tinge along distal borders.
Scutal growth lines without longitudinal striations; articular ridge absent; pit for adductor
muscle small, round. Tergum with shallow, wide furrow running from apex to base. Cirrus I
with unequal (anterior ramus: 7-segmented; posterior ramus: 15-segmented). Cirrus II with
rami subequal (anterior ramus: 11-segmented; posterior ramus: 12-segmented). Cirri I and II
with very dense, long setae on surface areas. Cirri III–VI with rami slightly subequal, rounded.
Penis sturdy, not long. Labrum with very shallow notch, three or four irregularly arranged,
blunt teeth on each side. Maxillule with distinct, narrow notch with two large setae on upper
side. Mandibles with five teeth, second and third bifid and fifth is rudimentary. Measurements
of five specimens: basal length of shell 6.23–12.08 mm, orifice length 4.63–9.62 mm, carinal
height 3.48–13.22 mm, orifice width 3.24–6.33 mm, basal width 5.66–9.56 mm.
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Chapter 3
Distribution:
Manado Bay (Indonesia); Japan (Broch 1931–1932). In this study, Solidobalanus pseudauricoma
was found at Kei Islands and Tanimbar Island, Indonesia.
Type locality:
Manado Bay, Indonesia (1°31’N, 124°47’E), 500 m depth; Japan (32°25’N, 128°33’E), 400 m
depth (Broch 1931–1932).
Remarks:
In the type description, Broch (1931–1932) commented that the specimens were white,
without stripes. However, several of the specimens collected by KARUBAR had a pale pink tinge
and one specimen (from station DW22) had pale brownish-pink parietes with small, narrow
ellipsoidal whitish spots, the latter orientated longitudinally, thus producing a reticulated
effect. Radii whitish with pink tinge along distal borders.
Figure 3.36. Solidobalanus pseudauricoma (Broch, 1931) (MNHN-IU-2019-4818). (a) left lateral view,
(b) orifice view, (c) basal view, (d) right lateral view. Scale bars: a–d) 4 mm.
Genus Striatobalanus Hoek, 1913
Balanus Section F (part.) Darwin, 1854: 277.
Chirona Gray, 1825: 37. — Pilsbry 1916: 204.
Balanus (Striato-Balanus) Hoek, 1913: 159, 179.
Chirona (Striatobalanus). — Newman & Ross 1976: 23, 50 (as subgenus).
Striatobalanus. — Jones 2004: 150.
Diagnosis:
Parietes and radii without pores; radii usually narrow; scutum striated longitudinally with
adductor ridge; tergum furrow deep; cirrus I with rami very unequal in length.
Type species:
Balanus amaryllis var. (a), var. (b) Darwin, 1854: 279, pl. 7, figs 6a–c.
Type locality:
Unknown.
Deep-sea barnacles from the Karubar expedition (1991)
139
Striatobalanus amaryllis (Darwin, 1854)
Figure 3.37
Balanus amaryllis var. (a), var. (b) Darwin, 1854: 279, pl. 7, fig. 6a–c. — Hoek 1913: 179, pl. 15, figs
17–21, pl. 16, figs 1–4. — Pilsbry 1916: 217. — Nilsson-Cantell 1925: 329. — Hiro 1936: 624. —
1939c: 243. — Stubbings 1936: 174. — Utinomi 1962: 216. — 1969: 88.
Balanus amaryllis dissimilis Lanchester, 1902: 369, pl. XXXIV, fig. 3–3C, with var. clarovittata Lanchester,
1902: 370.
Balanus amaryllis var. a (= Balanus roseus Lamarck, 1818). — Gruvel 1905: 250, with var. b (niveus). —
Gruvel, 1905: 250 (= Balanus amaryllis var. b Darwin, 1854: 279)).
Balanus (Chirona) amaryllis. — Nilsson-Cantell 1921: 329, pl. 3, fig. 9. — Rosell 1981: 302.
Balanus amaryllis forma euamaryllis. — Broch, 1922: 321.
Balanus amaryllis forma laevis Broch, 1931: 67, fig. 24.
Balanus sp. Dong & Mao, 1956: 290, fig. 8.
Chirona (Striatobalanus) amaryllis. — Newman & Ross 1976: 50.
Chirona amaryllis. — Foster 1981: 350.
Balanus amaryllis. — Dong et al. 1982: 95.
Balanus amaryllis euamaryllis Dong et al. 1982: 96, figs A–C.
Chirona amaryllis: Zevina et al. 1992: 72, fig. 49.
Striatobalanus amaryllis. — Jones 2004: 150. — Chan et al. 2009b: 222, 190–192.
Material examined:
Tanimbar Island: 1 specimen (only shell), MNHN-IU-2019-4814, Stn. CP 65, 09°14’S,
132°27’E, 174–176 m depth, 1 November 1991, attached to broken sea urchin shell.
Diagnosis:
Shell conical; orifice large, pentagonal, toothed; shell colour pinkish, lighter transparent stripes
run longitudinally over surface; radii very narrow, reddish, upper margins very oblique; alae
broader, lighter colour.
Description:
Shell conical; tips of rostrum and carina slightly curved inwards. Orifice large, pentagonal,
toothed. Colour yellowish-white, with slightly darker longitudinal lines on main parts of plates.
Radii with very oblique summits, broadest a little distance from the orifice, narrower towards
basis. Alae broader than radii, summits rounded. Specimen without scutum, tergum and soft
parts. Measurements of specimen: basal length of shell 16.76 mm, orifice length 9.13 mm,
carinal height 9.89 mm, orifice width 7.24 mm, basal width 15.14 mm.
Distribution:
Arafura Sea, Indonesia. Indo-west Pacific: South and East Africa, Indian Ocean; Australia
(N); Singapore; Malay Arch.; Cambodia; Gulf of Thailand; Mouth of Bassac; Vietnam (Bay of
Nhatrang; Lien Chien, Tourane; Codor Is); Hong Kong; S China Sea; China; Philippines; Taiwan;
Japan (S); attached to rocks, gastropod shells, rusting iron, fouling species; sublittoral, 5–500
m depth (Jones and Hosie 2016). In this study, Striatobalanus amaryllis was found at Tanimbar
Island, Indonesia.
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Chapter 3
Type locality:
Unknown.
Figure 3.37. Striatobalanus amaryllis (Darwin, 1854) (MNHN-IU-2019-4814). (a) orifice view, (b) right
lateral view. Scale bars: a, b) 3 mm.
Striatobalanus tenuis (Hoek, 1883)
Figure 3.38
Balanus tenuis Hoek, 1883: 154, pl. 13, figs 29–33. — 1913: 190, pl. 17, figs 14–19, pl. 18, fig. 1. —
Gruvel 1905: 247, fig. 275. — Pilsbry 1916: 216. — Barnard 1924: 74. — Nilsson-Cantell 1925: 34,
fig. 13, pl. 1, figs 5–6. — 1927: 785. — 1938: 46. — Broch 1931: 70. — Hiro 1937b: 439, fig. 24. —
Utinomi 1962: 216. — 1968: 174. — 1969: 88, fig. 6. — Utinomi & Kikuchi 1966: 6.
Balanus albus Hoek, 1913: 185, pl. 16, figs 12–13, pl. 17, figs 1–6. — Stubbings 1936: 41, fig. 18.
Chirona (Striatobalanus) tenuis. — Newman & Ross 1976: 50. — Rosell 1989: 33, pl. 10g. — 1991: 38.
Balanus (Chirona) tenuis. — Ren & Liu 1978: 161, fig. 22, pl. 7, figs 6–10. — Nilsson-Cantell 1921: 302.
Balanus tenuis. — Dong et al. 1982: 97, fig. A–C.
Chirona tenuis. — Zevina et al. 1992: 74, fig. 50.
Striatobalanus tenuis. — Jones 2004: 152. — Liu & Ren 2007: 363, fig. 161. — Chan 2009: 74, fig. 2H
and fig. 23A–G.
Material examined:
Kei Islands: 5 specimens, MNHN-IU-2019-4820, Stn. CC 10, 05°21’S, 132°30’E, 329–389
m depth, 23 October 1991; 1 specimen (shell only), MNHN-IU-2019-4835, Stn. CP 16,
05°17’S, 132°50’E, 330–350 m depth, 24 October 1991.
Tanimbar Island: 1 specimen, MNHN-IU-2019-4832, Stn. DW 49, 08°00’S, 132°59’E, 206–
2010 m depth, 29 October 1991; 1 specimen (shell only), MNHN-IU-2019-4833, Stn. CP 65,
09°14’S, 132°27’E, 174–176 m depth, 1 November 1991; 4 specimens (shell only), MNHNIU-2019-4834, Stn. CP 79, 09°16’S, 131°22’E, 239–250 m, 3 November 1991; 1 specimen
(only shell), MNHN-IU-2019-4813, Stn. CP 85, 09°22’S, 131°14’E, 240–245 m, 4 November
1991, attached to shell of gastropod; 2 specimens, MNHN-IU-2019-4819, Stn. CP 86, 09°26’S,
131°13’E, 223–225 m depth, 4 November 1991, attached to gastropod shells.
Deep-sea barnacles from the Karubar expedition (1991)
141
Diagnosis:
Shell colour whitish to yellowish; surface smooth, glossy; orifice pentagonal shaped, deeply
toothed; radii narrow, summits very oblique, slightly concave; basis solid; scutum with
longitudinal striations; tergum with short, rather broad spur.
Description:
Shell with six plates, conical; orifice large, distinctly toothed; radii well-developed; basis thin,
ribbed, solid. Scutum triangular, not elongated, with longitudinal striations; tergum slightly
beaked, exhibiting traces of longitudinal striations, spur short, slightly narrow, depressor
crests distinctly developed. Cirri I to VI with rami slightly equal in length, transparent and
orange-coloured. Penis rather long, sparse, minute hairs on the surface, tapering towards tip,
distinctly hairy at tip. Maxillule with edge slightly straight, all the setae similar sized. Mandibles
with five teeth, fourth and fifth small. Measurements of five specimens basal length of shell
18.92–28.30 mm, orifice length 13.78–16.29 mm, carinal height 18.02–20.24 mm, orifice
width 10.15–13.70 mm, basal width 20.51–28.03 mm.
Distribution:
Arafura Sea, Indonesia; Indo-west Pacific: S Africa; Persian Gulf; Indian Ocean; Australia (N);
Singapore; Malay Arch.; Vietnam; Hong Kong; S China Sea; E China Sea; Philippines; Taiwan;
Japan (S); W Pacific; attached to crabs, gastropod, bivalve, shells solitary coral, bark of coconut,
gorgonians, antipatharians, stones; 7–551 m depth (Jones and Hosie 2016). In this study,
Striatobalanus tenuis was found at Kei Islands and Tanimbar Island, Indonesia.
Type locality:
West of Mindoro, Philippines; 6°8’N, 121°19’E; depth: 275 m, at coral-bottom (Hoek 1913).
Figure 3.38. Striatobalanus tenuis (Hoek, 1883) (MNHN-IU-2019-4820). (a) orifice view, (b) left lateral
view, (c) right lateral view, (d) basal view. Scale bars: a–d) 10 mm.
Family Pyrgomatidae Gray, 1825
Balanidae Leach, 1817: 68 (part.). — Darwin 1854: 33 (part.).
Balaninae. — Nilsson-Cantell 1921: 306 (part.).
Pyrgomatidae Gray, 1825: 102. — Newman 1996: 503.
Pyrgomatinae. — Ross & Newman 1973: 149.
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Chapter 3
Diagnosis:
Wall of four plates wholly concrescent; parietes solid or tubiferous; when tubiferous, tubes occur
between outer lamina and sheath or between external ribs of wall; interlaminate figures complex,
essentially arborescent; radii solid; basis calcareous, rarely tubiferous, membranous in Pyrgopsella.
Subfamily Megatrematinae Holthuis, 1982
Bosciinae Newman & Ross, 1976: 59.
Megatrematinae Holthuis, 1982: 319.
Diagnosis:
Wall of four plates wholly concrescent; opercular valves normal; tergum with weakly developed
lateral depressor muscle crests or crests lacking; when shell concrescent, sheath with paired sulci.
Type genus:
Megatrema Sowerby, 1823, by original designation (Holthuis 1982).
Tribe Pyrgomini Ross & Pitombo, 2002
Pyrgominini Ross & Pitombo, 2002: 58.
Type genus:
Pyrgomina Baluk & Radwanski, 1967.
Genus Pyrgomina Baluk and Radwanski, 1967
Pyrgomina Baluk and Radwanski, 1967b: 691, pl. 1–2.
Type species:
Pyrgomina seguenzai Baluk and Radwanski, 1967a: 485.
Type locality:
Pliocene (Piacenzian Stage); Gournes, Iraklion nomarchia, Island of Crete, Greece, 35°06’N,
25°47’E; host coral unknown (Ross and Pitombo 2002).
Pyrgomina sp.
Figure 3.39
Material examined:
Tanimbar Island: 1 specimen, MNHN-IU-2019-4817, Stn. CP 86, 09°26’S, 131°13’E, 223–
225 m depth, 4 November 1991, attached to coral.
Diagnosis:
Shell purple-pinkish, tall, conical; oriface oval; base permeated by pores, expanded out of the
coral; tergum and scutum subtriangular.
Description:
Shell stands exserted on the coral, externally furnished with ribs, lower part rounded with
radiating ribs; oriface oval, small and narrow. Scutum triangular, basal margin curved; tergum
Deep-sea barnacles from the Karubar expedition (1991)
143
triangular with narrow spur. Cirrus I with rami unequal (anterior ramus: 10-segmented;
posterior ramus: 15-segmented); posterior ramus of cirrus IV without spines. Maxilla globular;
maxillule not notched, eight large setae on straight cutting edge; mandible with five major
teeth. Measurements of specimen: basal length of shell 7.46 mm, orifice length 2.42 mm,
carinal height 10.45 mm, orifice width 1.41 mm, basal width 6.15 mm.
Distribution:
Pyrgomina sp. was found at Tanimbar Island, Indonesia.
Figure 3.39. Pyrgomina sp. (MNHN-UI-2019-4817). (a) orifice view, (b) left lateral view, (c) right lateral
view. Scale bars: a–c) 2 mm.
Family Balanidae Leach, 1817
Balanidae Leach, 1817: 68. — Gray 1825: 104. — Darwin 1854: 33 (part.). — Pilsbry 1916: 48. —
Nilsson-Cantel 1921: 306. — Newman & Ross, 1976: 59. — Foster 1978: 95. — Buckeridge 1983:
103. — Newman 1996: 503. — Pitombo 2004: 262. — Chan et al. 2009b: 229.
Diagnosis:
Shell with four or six plates; parietes tubiferous; tubes arranged in single uniform row formed
between inner and outer lamina, supplementary tubes may form basally; interlaminate figures
complex, arborescent; radii solid or tubiferous, more-or-less developed; basis calcareous,
commonly tubiferous; caudal appendages absent; penis commonly with basidorsal point.
Subfamily Amphibalaninae Pitombo, 2004
Amphibalaninae Pitombo, 2004: 263.
Diagnosis:
Shell with four or six plates; parietal tubes with one or more rows, commonly transverse
septa; radii with transverse teeth on sutural edge with denticles on lower side only; alae not
cleft; basis with single tubiferous; scutum with conspicuous adductor ridge; tergum with welldeveloped depressor muscle crests, growth lines in tergum spur display an obvious change in
direction; second maxilla with smooth anterior margin of distal lobe, acuminate setae with
enlarged, modified tips.
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Chapter 3
Genus Amphibalanus Pitombo, 2004
Amphibalanus Pitombo, 2004: 263.
Diagnosis:
Shell with four or six plates; parietal tubes with one or more rows, commonly transverse
septa; radii with transverse teeth on sutural edge, with denticles on lower side only; alae not
cleft; basis with single tubiferous; scutum with conspicuous adductor ridge; tergum with welldeveloped depressor muscle crests, growth lines in tergum spur display an obvious change
in direction; second maxilla with smooth anterior margin of distal lobe, acuminate setae
with enlarged, modified tips. Cirrus III with inner face of endopod with pinnate setae, rarely
bifurcate (complex) setae.
Type species:
Balanus amphitrite Darwin, 1854: 240 (part.), pl. 5, figs 2a–d, i–k, m–o; type locality: Natal,
South Africa.
Amphibalanus amphitrite (Darwin, 1854)
Figure 3.40
Balanus amphitrite Darwin, 1854: 240 (part.), pl. 5, figs 2a-d, i-k, m-o. — Weltner 1897: 264. — Hoek
1913: 167. — Pilsbry 1916: 89. — Zevina et al. 1992: 89, fig. 61. — Puspasari et al. 2001: 7.
Balanus amphitrite var. (1) communis Darwin, 1854: 240, pl. 5, fig. 2e, h, l.
Balanus amphitrite communis. — Nilsson-Cantell 1921: 311, fig. 64.
Balanus amphitrite forma hawaiiensis Broch, 1922: 314, fig. 56 (part.).
Balanus amphitrite forma denticulata Broch, 1927b: 133, fig. 14 (part.).
Balanus amphitrite hawaiiensis. — Hiro 1937c: 432, figs 20, 21.
Balanus amphitrite cochinensis Nilsson-Cantell, 1938b: 43, fig. 11a–e.
Balanus amphitrite var. fluminensis Oliveira, 1941: 21, pl. 4, fig. 4, pl. 5, figs 1, 2, pl. 8, figs 1–5.
Balanus amphitrite var. aeratus Oliveira, 1941: 22, pl. 4, fig. 5, pl. 9, figs 1–4.
Balanus amphitrite herzi Rogers, 1949: 28, pl. 1, figs 6, 12–15.
Balanus amphitrite franciscanus Rogers, 1949: 29, pl. 1, figs 5, 7, 16–19.
Balanus amphitrite var. columnarius Tarasov & Zevina, 1957: 179, 184, fig. 68 a–e.
Balanus amphitrite denticulata Henry, 1959: 192, pl. 1, fig. 5, pl. 3, fig. 7, upper row right.
Balanus amphitrite amphitrite. — Harding 1962: 274, pl. 1a–g, pl. 2a–k. — Dong et al. 1982: 90, fig.
A-E. — Rosell 1981: 302.
Balanus amphitrite var. Hawaiiensis. — Stubbings 1963b: 15.
Amphibalanus amphitrite. — Pitombo 2004: 263, 274, figs 2A, B, 7A, B, 8C. — Chan et al. 2009b: 241.
— Chen et al. 2014: 1071. — Pochai et al. 2017: 27, fig. 9. — Pitriana et al. 2020a: 42, fig. 21.
Material examined:
Tanimbar Island: 4 specimens, MNHN-IU-2019-4815, Stn. CP 52, 08°03’S, 131°48’E,
1244–1266 m depth, 30 October 1991.
Deep-sea barnacles from the Karubar expedition (1991)
145
Diagnosis:
Primary parietal tubes with transverse septa; exterior of shell with longitudinal purple striations,
horizontal striations absent; tergum short with wide spur; cirri III–VI with erect teeth below
posterior angles of distal; cirrus III without complex setae.
Description:
Shell six-plated, conical, round; externally smooth, white with groups of well-spaced, dark
purple vertical stripes; horizontal striations on shell surface absent. Interior of paries with
single row of tubes; radii solid, wide; alae with summits moderately oblique; basis porose,
calcareous. Scutum externally striped, occludent margin toothed, lateral depressor muscle pit
small. Tergum with spur wider than long, less than its own width from basi-scutal angle. Cirrus
I with unequal rami (anterior ramus: 10-segmented; posterior ramus: 16-segmented). Cirrus
II with slightly equal rami (anterior ramus: 10-segemented; posterior ramus: 12-segmented).
Cirrus III with rami subequal (anterior ramus: 12-segmented; posterior ramus: 13-segmented.
Cirri IV–VI with rami subequal, longer, more slender. Penis moderately long, tapering distally,
sparse, minute hairs on surface, distinctly hairy distally. Labrum deeply notched, numerous
teeth on each side. Maxilla without notch below upper pair of spines, nine spines between
upper and lower pairs, few short spines on inferior angle; lower pair of spines usually on slight
prominence, which may be sometimes moderately strong or strong. Mandible with five teeth,
inferior angle with second tooth bifid. Measurements of four specimens: basal length of shell
5.27–12.10 mm, orifice length 3.81–7.89 mm, carinal height 3.64–5.65 mm, orifice width
2.72–5.22 mm, basal width 4.62–12.03 mm.
Distribution:
Cosmopolitan in tropical and subtropical waters. Bermuda & SE USA to Brazil; England and
West Europe to South coast of Africa Red, Black and Mediterranean Seas; Suez Canal; SE
Africa; Indian Ocean; Australia; Singapore; Malaysia; Réam (Cambodia); Gulf of Siam; Vietnam;
Condor Island; Tang Trien (South Annam); Cauda Nhatrang; Hongay, Tonkin; S China Sea;
Hong Kong; China; Bohai Sea; Taiwan; Philippines; Japan; South Honsyu, Kyusyu & Ryukyu
Island; Vladivostok; Hawaii; central California to SW Mexico; fouling species; lower littoral to
sublittoral (Jones and Hosie 2016). Recently, also recorded from Ambon Island and Saparua
Island, Indonesia (Pitriana et al. 2020a). In this study, Amphibalanus amphitrite was found at
Tanimbar Island, Indonesia.
Type Locality:
Natal, on a piece of bamboo (Darwin 1854).
Remarks:
Known as an important fouling species of ships and marine installations. The suggestion of
anti-fouling paint on the bases of the specimens examined suggests that these specimens
were probably knocked off the ship during trawling operations, explaining the great depth at
which these specimens were collected, as the normal depth range is 0–9 m.
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Chapter 3
Figure 3.40. Amphibalanus amphitrite (Darwin, 1854) (MNHN-IU-2019-4815). (a) orifice view, (b) right
lateral view, (c) basal view. Scale bars: a–c) 4 mm.
3.5 Discussion
Prior to the Karubar expedition, 24 species of barnacles had been collected from the Kei
Islands and Aru Island by the Siboga expedition (Hoek 1913). Other pertinent reference works
to the barnacles from these islands are Jones et al. (2001) and Jones and Hosie (2016), who
recorded 15 species from the Kei Islands and Aru Island.
In addition to the works of Hoek (1913), Jones et al. (2001) and Jones and Hosie (2016),
Broch (1931–1932) reported on 67 species of barnacles collected by the Danish expedition to
the Kei Islands (1922) and deposited in the Zoological Museum of Copenhagen University. In
his report, only four species, Euscalpellum rostratum (Darwin, 1851), Lepas (Anatifa) anatifera
Linnaeus, 1758, Conchoderma virgatum Spengler, 1789 and Acasta dentifer (Broch, 1922),
were explicitly collected in the Kei Islands. The other barnacle species recorded were collected
at other places along the route of this expedition, such as Lampung Bay, Krakatau, Java Sea,
Sunda Strait, Makassar Strait, Tual, Banda Neira, Ambon and Saparua Bay.
The lists of Hoek (1913), Broch (1931–1932), Jones et al. (2001) and Jones and Hosie
(2016) record a total of 25 species from the Kei Islands, Aru Island and Tanimbar Island. The
results currently recorded herein reveal that 40 species are now recorded from these Islands.
Of the 40 species herein, ten are recorded in previous studies: Megalasma striatum
Hoek, 1883 (listed in Hoek (1913)); Scalpellum stearnsi Pilsbry, 1890 (listed in Hoek (1913));
Annandaleum japonicum (Hoek, 1883) (listed in Hoek (1913)); Annandaleum laccadivicum
(Annandale, 1906) (listed in Hoek (1913)); Regioscalpellum moluccanum (Hoek, 1883) (listed in
Hoek (1913)); Altiverruca navicula (Hoek, 1913) (listed in Hoek (1913) and Buckeridge (1994));
Newmaniverruca albatrossiana (Pilsbry, 1912) (listed in Hoek (1913) and Buckeridge (1994));
Hexelasma arafurae Hoek, 1913 (listed in Hoek (1913) and Jones & Hosie (2016)); Solidobalanus
auricoma (Hoek, 1913) (listed in Hoek (1913) and Jones et al. (2001)); Striatobalanus tenuis
(Hoek, 1883) (listed in Hoek (1913) and Jones and Hosie (2016)).The remaining 30 species can,
therefore, be considered as new records for the Kei Islands and Tanimbar Island.
Deep-sea barnacles from the Karubar expedition (1991)
147
The present study and previous works on the barnacles of the Kei Islands, Aru Island
and Tanimbar Island, especially the works of Hoek (1883, 1907, 1913), Broch (1922, 1931–
1932), Buckeridge (1994, 1997), Jones et al. (2001) and Jones and Hosie (2016), enrich our
knowledge of the barnacle fauna of these islands. This study demonstrates once more the
value of museum collections as a resource in biodiversity science.
The result of this study also strengthens the statement of Hoeksema (2007) that the
Indo-Malayan region (which extends from East Indonesia to the Philippines and the Solomon
Islands) is a centre of maximum marine biodiversity. Darwin (1854) demonstrated that this
area had greater species richness than elsewhere in the world at the time. He named it the East
Indian Archipelago (including the Philippines, Borneo, New Guinea, Sumatra, Java, Malacca
and the eastern coast of India) and categorised it as his third province of barnacles. In this
province, he found 37 barnacle species, the largest number known at that time, compared
with the other provinces.
Regarding the biodiversity of barnacles, the Indo-Malayan region as the centre of
benthic biodiversity has not been replaced by other areas. In recent times, many studies and
expeditions have been conducted in this area, revealing many more species of barnacles. For
example, three expeditions have been undertaken within Philippine waters from 1976 until
1985 through MUSORSTOM Cruises and the collections the U.P. Marine Biological Laboratory
at Puerto Galera, Oriental Mindoro (Rosell 1991; Chan 2009). Overall, the three of scientific
cruises of MUSORSTOM collected 78 species of barnacles, 43 of which are new records and 12
species are new to science (Rosell 1991). Through the Philippine Panglao expedition (2005),
Chan (2009) has also increased the number of barnacles from the Philippines, reporting 20
barnacle species with two new to science.
Similar to the Philippine waters, eastern Indonesian waters also have a high diversity of
barnacles. Recently, it has been revealed that the Moluccan Islands in eastern Indonesia have
97 species of barnacles, 23 of which are new records and two species are still awaiting their
species descriptions (Pitriana et al. 2020a). Furthermore, this number will increase with the
results of the study of the barnacles from Karubar expedition (1991) that have revealed 40
species of barnacles.
The results of the studies of barnacles from the Philippines and eastern Indonesian
waters reconfirm the Indo-Malayan region as the epicentre of marine biodiversity.
3. 6 Acknowledgements
The first author would like to thank Prof. Frank Riedel from the Freie Universität Berlin
for his general support and Bani Wahyudi and Abdul Basit for their support and comments on
picture editing. Thanks to Bernhard Schurian for supporting the morphological work at the
Museum für Naturkunde Berlin. We would like to thank Dr. Martin Husemann, Prof. Benny
Kwok Kan Chan and anonymous reviewer for valuable comments on the manuscript.
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The first author would also like to thank Prof. Keiji Baba, Dr. Enrique Macpherson, Ferran
Palero, PhD, Paula Martin-Lefèvre and Sébastien Soubzmaigne for the great time working in
the Crustacean Laboratory of the Muséum National d’Histoire Naturelle (MNHN), Paris and to
Thomas von Rintelen (MfN Berlin) for providing funds for the research visit to Paris.
The second author would like to acknowledge Dr Alain Crosnier (retired, MNHN) for his
generous support to enable her study of the KARUBAR cirripede collection in Paris.
This study was funded by the Ministry of Research, Technology and Higher Education,
the Republic of Indonesia within the Program for Research and Innovation in Science and
Technology (RISET-Pro), World Bank Loan No. 8245-ID.
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CHAPTER 4
Exploring sponge-inhabiting barnacles of eastern Indonesia
using micro-CT scanning
Pipit Pitriana1, 2, 3, Andreas Wessel1, Tina Aschenbach1, Kristina von Rintelen1
Museum für Naturkunde – Leibniz Institute for Evolution and Biodiversity Science,
Invalidenstrasse 43, 10115 Berlin, Germany
2
Research Centre for Deep-sea, Indonesian Institute of Science (LIPI), Jl. Y. Syaranamual, Poka,
Tlk. Ambon, Kota Ambon, Maluku, Indonesia
3
Institute of Geological Sciences, Freie Universität Berlin, Malteserstrasse 74–100 Building C
and D, 12249 Berlin, Germany
1
submitted to: journal of Treubia
4.1 Abstract
We present a morphological study of Indonesian sponge-inhabiting barnacles using standard
light microscopy in combination with micro-CT scanning and computer-aided 3D-reconstruction of the
external shell morphology. A taxonomic analysis of the material detected four different genera of sponges
inhabited by five different species of balanomorph barnacles, two of which are undescribed. Together
with conventional morphological examination by dissection, we provide modern non-destructive
imaging methods, using micro-CT scanning to enhance our knowledge of the morphological characters
of sponge-inhabiting barnacles from eastern Indonesia. Although there were some methodological
limitations regarding the contrast-enhancing technique, this study demonstrates micro-CT as a useful
non-destructive technique of integrative taxonomy, for the examination of sponge-inhabiting barnacles.
4.2 Introduction
Barnacles (Cirripedia) are sessile crustaceans where the adult forms are structurally and
biologically diverse, and permanently attached to hard substrata or to other living organisms
(Newman & Abbott, 1980; Høeg et al., 2015). It comprises three orders, the Thoracica or ‘true
barnacles’ (including Sessilia and Pedunculata), the Acrothoracica (burrowing barnacles), and
the Rhizocephala (parasitic barnacles) (Høeg et al., 2015). Some members of the suborder
Balanomorpha selectively choose invertebrates or vertebrates as their hosts, ranging from sponges
to whales (Foster, 1978). Many epizoic barnacles burrow into, or are overgrown, by the host’s tissue
(Anderson, 1994). Members of subfamily Acastinae and genus Membranobalanus are examples of
epibiotic barnacles. Acastinae are obligate symbionts of Porifera and Cnidaria, but also be found in
association with gorgonians, alcyonarians, or antipatharians; while Membranobalanus are obligate
symbiont of Porifera (Kolbasov, 1993; Van Syoc et al., 2015; Yu et al., 2017).
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Most species of Acastinae have spines or projections of various lengths on the external
surface of the parietes, penetrating as anchors into the sponge tissue (Kolbasov, 1993). In
addition, according to Van Syoc et al. (2015) the Acastinae with membranous bases may often
be distinguished from Membranobalanus spp. by the presence of these calcareous projections
on the shell wall. These calcareous projections can be used to differentiate members of the
Acastinae and Membranobalanus. However, the spines often break away from the shell wall
when specimens are removed from their host sponge, leaving only “pores” in the shell wall
marking the site of attachment (Van Syoc et al., 2015).
The Acastinae of eastern Indonesia have been studied by Hoek (1913) and Broch (1931–
1932) and both noted calcareous projections on the surface of the shell wall. For Acasta conica,
Hoek (1913: 235) stated “the surface of the parietes is smooth in the lower half, covered in
the upper half with rounded scale-like patches, the exact nature of which I have not been
able to discover”, and likewise Broch (1931–1932: 98) for Acasta foraminifera, “the white
shell is anchored in the sponge tissue by rather large spines; it is, however, almost absolutely
impossible to prepare away the tissue of the sponge without removing also every such spine,
so intimate is the connection with the extremely hard tissue of the sponge”.
Modern non-destructive imaging methods, such as X-ray micro-computed tomography (microCT), are particularly well suited to overcome the problems of traditional challenges associated with
the size and fragility of small invertebrates (Faulwetter et al., 2013; Semple et al., 2018). Studies
on invertebrates utilising 3D-imaging tools now encompass a wide range of applications, including
for comparative, developmental and functional morphological research (Metscher, 2009a; Semple
et al., 2018). However, the widespread application of micro-CT imaging has only been used in a
few studies of barnacles to date. For example, Noever et al. (2015) studied the rhizocephalan root
system of parasitic barnacles in their crusteacan host. Hosie et al. (2019) described a new species of
sponge-inhabiting barnacle (Membranobalanus porphyrophilus) from Australia and presented an
interactive, 3D micro-CT derived volume reconstruction of the designated holotype.
In this study, we present the use of micro-CT scanning and 3D reconstruction as a nondestructive method to visualize and count sponge-inhabiting barnacles inside their host.
Secondly, we provide a conventional morphological study of the same barnacle specimens
extracted from their host, using light microscopy. Our aim is to demonstrate the potentials
use of micro-CT imaging in barnacle research, and to develop an approach for a practicable
combination of different morphological techniques.
4.3 Material and Methods
4.3.1 Material
Sponge and barnacle specimens examined in this study were collected by the first
author between April 2016 and September 2017 at Saparua Island and South East Sulawesi,
Indonesia (Table 1). All sponges were photographed using a digital camera (DSLR Nikon D200).
Exploring sponge-barnacle using micro-CT scans
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Specimens were then fixed and stored in 96% ethanol and transferred into 75% ethanol for
long-term preservation. In total, 14 lots containing four genera of sponge inhabiting barnacles
were collected from seven localities.
4.3.2 Specimen preparation, micro-CT, and 3D reconstruction
Specimen preparation
To prepare the samples for micro-CT scanning, sponge specimens were removed from
the ethanol and put into dry containers. To avoid movement, the sponges were held in place
using cotton. The cotton was soaked in a small amount of ethanol to prevent specimens from
drying and thereby shrinkage, which can occur if wet objects are left in a dry environment for
a certain amount of time (Metscher, 2009b; Buytaert et al., 2014).
Staining method to enhanced micro-CT imaging of soft body of barnacles
We used Lugol’s solution (one part iodine and two parts potassium iodide in an aqueous
solution) to stain the soft body of barnacle specimens. To produce a concentration of 0.3%,
0.1% (w/v) iodine and 0.2% (w/v) potassium iodide were dissolved in double-distilled water
(Heimel et al., 2019). To scan the soft body, one barnacle for each species was removed from
the host sponge. Barnacle specimens were soaked in Lugol’s solution for seven days. After
seven days, the barnacles were thoroughly rinsed in tap water and put into a dry tube for the
scanning process.
Micro computer tomography (micro-CT)
Complete sponge specimens (one sponge for each species) were scanned using X-ray
imaging at the Museum für Naturkunde Berlin, Germany, with the Scanner FF35 (Yxlon,
Hamburg). Raw files were produced with the help of the software VGStudio Max (Volume
Graphics, Heidelberg, Germany). Scanning occurred with the following parameters: 80 kV, 15
W, 16 μm voxel size, 2000 projections/turn helical CT.
3D reconstruction of barnacles inside the sponges
The software Amira 6.0.0 was used for the 3D reconstruction of the raw files. After
creating a new project, the raw files were imported into the workspace. To visualize the dataset,
the “Volren” tool was applied, which is a volume renderer. In the segmentation mode, the
magic wand tool was used to segment the barnacles located inside the sponges. Subsequently,
every segmented barnacle was assigned a different “label”, making it possible to differentiate
between single barnacles by assigning different colours to individual labels. Switching back to
project mode and using the “Volren” volume renderer, the opacity of the surrounding medium
was adjusted, so that the barnacles are clearly visible. For better visualization purposes,
“Specular” was used for shading and the “Ortho” tool was applied to crop the volume and
remove part of the plastic container and surrounding matrix.
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4.3.3 Specimen direct morphological examination
After specimens were scanned using micro-CT, one to three barnacle specimens were
removed from each sponge. Based on the overview scans showing all barnacle specimens
situated in each sponge, we could estimate which parts of the sponge to dissect to obtain a
specific barnacle specimen. Morphological characters of the hard body parts (parietes and
opercular plates) and soft body parts (mouthparts, six pairs of cirri, and penis) were examined
as in Pitriana et al. (2020a). Species were identified based on Lamarck (1818), Darwin (1854),
Krüger (1911), Hoek (1913), Broch (1931), Rosell (1991), and Kolbasov (1993).
Hard body parts were separated from soft body parts using a scalpel. Shell plates were
separated and cleaned with a bleach solution to remove any organic material, rinsed with
fresh water, dried and observed under a stereo microscope (Leica M125) and photographed
with a digital camera (Leica Microsystems M205C).
Mouthparts (labrum, palps, maxillae, maxillules, and mandible) were dissected using a
scalpel, each was mounted on a glass slide and examined under a light microscope (Carl Zeiss
Axioskop 20). The cirri were separated into couples of cirri I-VI and the penis, before being mounted
on glass slides. The anatomy of these soft body parts was studied under the same light microscope.
4. 4 Results
We found species of four different genera of sponges inhabited by five different species
of barnacles, two of them yet undescribed (Table 4. 1). Only the host sponge Axynissa sp. was
inhabited by two barnacle species, the others only by one barnacle species. Four of the sponge
barnacles had a calcareous basis and spines or projections calcareous on the external surface of the
parietes. Only the barnacle species Membranobalanus longirostrum had a membranous basis and
external surface of the parietes without spines or projections calcareous. The number of specimens
found in each host sponge varied between a minimum of six (Acasta sp. 1 in Axynissa sp.) and a
maximum of 23 barnacle specimens (Membranobalanus longirostrum in Agelas sp.) (Table 4. 1).
Table 4.1. Barnacle species and the host sponges from eastern Indonesia
Barnacle species
Host sponge species
Locality
Acasta sp. 1
Axynissa sp.
Pantai Marina
(Southeast Sulawesi)
Acasta sp. 2
Cinacyrella sp.
Desa Pia (Saparua Island)
Acasta sulcata Lamarck,
1818
Spongia sp.
Porto, Kulur, and Ihamahu
(Saparua Island)
Euacasta dofleini (Krüger,
1911)
Membranobalanus
longirostrum (Hoek, 1913)
Axynissa sp.
Agelas sp.
Teluk Haria, Porto, and Desa
Ihamahu (Saparua Island)
Teluk Haria (Saparua Island)
Exploring sponge-barnacle using micro-CT scans
153
Acasta sp. 1
The sponge Axynissa sp. found at South East Sulawesi Island (Table 4.1) was inhabited
by barnacle Acasta sp. 1 (Fig. 4.1a, b). In the micro-CT overview scan of Axynissa sp. we found
six specimens of barnacle inside the sponge. Barnacles were located near the surface layer of
their sponge hosts (Fig. 4.1c). The operculum looming high over the parietes (Fig. 4.1d).
Figure 4.1. Acasta sp. 1. (MZB Cru Cir 183) and its host sponge a) sponge of Agelas sp. b) close-up of
sponge Agelas sp c) position of Acasta sp. 1 in the host sponge d) shell view of Acasta sp. 1 Scale bars:
5 cm (a); 1 cm (b); 1 mm (c–d).
The color of the shell was yellowish with operculum soar almost as high as the parietes;
orifice was wide; plates externally with lots of calcareous spines (Fig. 4.2a–i). The basis was
quadrangular with horizontal growth ridges and the basal rim was crenated (Fig. 4.2e–g).
Furthermore, we disassemble the shell to observe each plate in detail. Scutum with horizontal
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growth ridges and occludental margin was toothed (Fig. 4.2h, i). Tergum apex was beaked,
with horizontal growth ridges was present, and the spur was broad (Fig. 4.2h, i).
Figure 4.2. Hard body of Acasta sp. 1 a) shell, right lateral view b) shell, left lateral view c) carinal view
d) rostral view e) basal view f) external view of plates g) internal view of plates h) external view of
scutum and tergum i) internal view of scutum and tergum. Scale bars: 1 mm (a–e); 0.5 mm (f–g); 0.25
mm (h–i).
We dissected the soft part of the barnacle to observe the mouthparts, six pairs of cirri,
and penis in detail. Cirrus I with rami very unequal in length, posterior margin with serrulate
and plumose setae, distal margins of terminal segments of anterior and posterior rami
with serrulate and bifid setae (Fig. 4.3a). Cirrus II with rami slightly unequal in length; the
posterodistal angle of segments of both rami with serrulate setae; distal margins of terminal
segments of anterior and posterior rami with serrulate and bifid setae (Fig. 4.3b). Cirrus IV
with rami unequal in length, hooks were present on the frontal margin of the segments of the
anterior ramus and basipod (Fig. 4.3c-e). Cirrus V with three small teeth at the frontal edge
Exploring sponge-barnacle using micro-CT scans
155
of proximal segments of the anterior ramus (Fig. 4.3f, g). Cirrus VI was long and slender with
equal rami in length (Fig. 4.3h). Penis was annulate and gradually tapering to the tip (Fig. 4.3i).
Maxillule cutting edge was straight without a notch, upper and lower margin with two big
stout simple setae (Fig. 4.3j); mandible with five teeth, inferior angle with five denticles, lower
margin of lateral angle with simple stout setae (Fig. 4.3k); labrum with V-shaped notch and
three teeth on each side of the crest (Fig. 4.3l).
Figure 4.3. Soft body of Acasta sp. 1 a) cirrus I b) cirrus II c) cirrus IV d) close-up view of cirrus
IV e) close-up view of hooks of cirrus IV f) cirrus V g) close-up view of cirrus V h) cirrus VI i)
penis j) maxillule k) mandible l) labrum. Scale bars: 200 µm (a–c, f, h, i); 100 µm (d, e, g, j–l).
Acasta sp. 2.
The sponge Cinacyrella sp. found at Saparua Island was inhabited by barnacle Acasta
sp. 2 (Fig. 4.4a). In the micro-CT overview scan of Cynacyrella sp. we found nine specimens of
barnacle inside the sponge. The barnacle Acasta sp. 2 lived in a group near the surface layer of
their sponge host with orifice facing the same direction to the surface of the host sponge (Fig.
4.4b, c). The barnacle with hole pattern all over the parietes, except on the operculum (Fig.
4.4d). The operculum plates with slightly horizontal growth ridges (Fig. 4.4e).
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Figure 4.4. Acasta sp. 2. (MZB Cru Cir 184) and its host sponge a) sponge of Cinacyrella sp. b) position
of Acasta sp. 2 in the host sponge c) orifice facing the same direction in the host sponge d) shell view of
Acasta sp. 2 e) internal view of the plates and operculum. Scale bars: 2 cm (a); 1 cm (b, c); 1 mm (d, e).
After the scanning process, we carefully tried to remove the barnacle from the host
and dissected it to verify the species of barnacle. However, we found the barnacle strongly
inherent in the sponge and make it very difficult to separate the barnacle with the sponge host
without breaking it. After trying several times, finally, we could separate barnacle from the
sponge host but with a lot of sponge tissue still attached to the shell of barnacle (Fig. 4.5a).
We found the basis consists of four separate plates (Fig. 4.5b). We immersed the barnacle
in 2% bleach to completely digest the sponge tissue, but we discovered that the bleach also
destroyed the shell of the barnacle (Fig. 4.5c). We immediately rinsed the barnacle with tap
water five times and air-dried it for species identification. The basis plates with horizontal
growth ridges and shallow radial furrows (Fig. 4.5d, e), and were crenate at the basal rim (Fig.
4.5f). Parietes externally with many holes as marks of attachment of the calcareous spines;
internally with longitudinal internal ribs extending from upper to lower part; radii and alae
with horizontal ridges (Fig. 4.5g, h). Scutum was semi-transparent and tinged with reddish
color at the upper part, horizontal growth ridges were present, occludental margin was slightly
toothed (Fig. 4.5i, j). Tergum was semi-transparent with horizontal growth ridges, the apex was
beaked and tinged with reddish color (Fig. 4.5i, j).
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Figure 4.5. Hard body of Acasta sp. 2 a) shell, lateral view b) shell, basal view c) shell view after immersed
in 2% bleach d) external view of the base plates e) internal view of the base plates f) close-up view of
basal rim g) external view of plates h) internal view of plates i) external view of scutum and tergum j)
internal view of scutum and tergum. Scale bars: 1 mm (a–c); 0.5 mm (d, e, g–h); 0.25 mm (f, i–l).
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We dissected the soft part of the barnacle to observe the mouthparts, six pairs of cirri,
and penis in detail. Cirrus I with rami very unequal in length, anterior and posterior margin
with thick serrulate and plumose setae, distal margin of terminal segments of both rami with
serrulate setae (Fig. 4.6a). Cirrus II with rami unequal in length, the posterodistal angle of
segments of both rami with serrulate setae, the distal margin of terminal segments of both
rami with serrulate and bifid setae (Fig. 4.6b). Cirrus III with rami slightly unequal in length,
similar to cirrus II, but slender and longer (Fig. 4.6c). Cirrus IV with hooks on the frontal margin
of the segments of the anterior rami and basipod; and two small teeth at the frontal edge
of proximal segments of the anterior ramus (Fig. 4.6d, e). Penis was annulate with sparse
hair distally, and it gradually tapering to the tip (Fig. 4.6f). Maxillule cutting edge was straight
without a notch, the upper and lower margin with two big stout simple setae (Fig. 4.6g);
mandible with five teeth, inferior angle with two stout denticles, lower margin of lateral angle
with simple setae (Fig. 4.6h); mandibular palp with dense setae on the upper region (Fig. 4.6i);
labrum with V-shaped notch and two small teeth on each side of the crest (Fig. 4.6j).
Figure 4.6. Soft body of Acasta sp. 2 a) cirrus I b) cirrus II c) cirrus III d) cirrus IV e) close-up view of
hooks of cirrus IV f) cirrus V g) maxillule k) mandible i) mandibular palp j) labrum. Scale bars: 200 µm
(a–d, f); 100 µm (e, g–j).
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Acasta sulcata Lamarck, 1818
The sponge Spongia sp. (Fig. 4.7a, b) found at Saparua Island was inhabited by the barnacle
species Acasta sulcata (Fig. 4.7c-e). In the micro-CT overview scan of Spongia sp. we found 13
barnacle specimens inside the sponge. Barnacles were located near the surface layer of their
sponge hosts (Fig. 4.7c), a video of Acasta sulcata in Spongia sp. was published in the MfN Data
Repository Berlin (shown in subchapter 4.7) (Pitriana et al., 2020b). The parietes were externally
smooth with calcareous spines attached in some areas (Fig. 4.7d) and ribbed internally (Fig. 4.7d,
e). The basis was cup-shaped and pointed at the central umbo (Fig. 4.7e).
Figure 4.7. Acasta sulcata Lamarck, 1818 (MZB Cru Cir 185) and its host sponge a) sponge of Spongia sp.,
b) Spongia sp. inhabited by Acasta sulcata, c) position of Acasta sulcata in the host sponge, d) calcareous
projections in the external surface parietes of Acasta sulcata, e) base of Acasta sulcata. Scale bars: 2 cm (a);
1 cm (b, c); 1 mm (d, e).
The color of the shell was whitish to yellowish with reddish patches towards the apex;
orifice was small and toothed (Fig. 4.8a–l). Parietes externally were smooth, with horizontal
growth ridges and calcareous spines; internally, at under sheath, with longitudinal ribs;
radii and alae with horizontal ridges (Fig. 4.8a–e). The basis was cup-shaped with basal rim
crenated; shallow radial furrows extending from center to the crenate basal rim (Fig. 4.8f).
Furthermore, we disassemble the shell to observe each plate in detail (Fig. 4.8g, h). We found
scutum with horizontal growth ridges, basal margin slightly concave in middle, occludental
margin toothed with tooth size increasing gradually from apex to base (Fig. 4.8i, j). Tergum
was semitransparent, apex beaked tinged with reddish color, internally scutal margin also with
reddish patches in the upper part, depressor muscle crests was absent, spur was broad, spur
furrow was shallow, and carinal margin was perpendicular to basal margin (Fig. 4.8k, l).
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Figure 4.8. Hard body of Acasta sulcata a) right side view b) left side view c) rostral view d) carinal
view e) upper view f) lower view g) external view of plates h) internal view of plates i) external view of
scutum j) internal view of scutum k) external view of tergum l) internal view of tergum. Scale bars: 1
mm (a–f); 0.5 mm (g–h); 0.25 mm (i–l).
We dissected the soft part of the barnacle to observe the mouthparts, six pairs of cirri,
and penis in detail. Cirrus I with outer ramus 80% of inner ramus in length; posterior margin
with serrulate and plumose setae; distal margins of the terminal segment of anterior and
posterior rami with serrulate and bifid setae (Fig. 4.9a). Cirrus II with rami unequal in length;
posterodistal angle of segments of both rami with thick serrulate setae; distal margins of the
terminal segment of anterior and posterior rami with serrulate and bifid setae (Fig. 4.9b).
Cirrus IV with rami unequal in length; there were hooks on the frontal margins of the segments
of the anterior rami and basipod (Fig. 4.9c). Cirrus V with two small teeth at the frontal edge
of proximal segments of the anterior ramus (Fig. 4.9d). Cirri V and VI with rami subequal in
length, terminal segments of anterior and posterior rami with serrulate setae. Maxillule cutting
edge was straight without a notch, upper and lower margin with numerous simple setae (Fig.
4.9e); mandible with five teeth, the second to fourth tooth were bifid, inferior angle with six
denticles and stout simple setae, upper margin of lateral angle with stout simple setae (Fig.
4.9f); labrum was bilobed, separated by V-shaped notch with three small teeth on each side
of the crest (Fig. 4.9g).
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Figure 4.9. Soft body of Acasta sulcata a) cirrus I b) cirrus II c) cirrus IV d) cirrus V e) maxillule f)
mandible g) labrum. Scale bars: 200 µm (a–d); 100 µm (e–g).
Euacasta dofleini (Krüger, 1911)
The sponge Axynissa sp. (Fig. 4.10a) found at Saparua Island (Table 4.1) was inhabited by
the barnacle species Euacasta dofleini (Krüger, 1911) (Fig. 4.10a). In the micro-CT overview scan of
Axynissa sp. we found 12 specimens with their opercula facing the same direction to the surface of
their sponge hosts (Fig. 4.10b). The shape of the shell base was quadrangular with four indistinct
and shallow radial furrows extending from the center to the crenate basal rim (Fig. 4.10c, d). The
calcareous spines were attached on the external surface of plates (Fig. 4.10d, e).
Figure 4.10. Euacasta dofleini (Krüger, 1911) (MZB Cru Cir 186) and its host sponge a) sponge of Axynissa
sp. b) position of Euacasta dofleini in the host sponge c) base of Euacasta dofleini d) close-up of the
base e) parietes with calcareous projections on the external surface. Scale bars: 3 cm (a); 1 mm (b–e).
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The color of the shell was yellowish with orifice toothed; wall plates have long and wormshaped calcareous projections on its external surface; the basis was saucer-shaped with basal
rim crenated (Fig. 4.11a–f). Furthermore, we disassemble the shell to observe each plate in
detail. Scutum with radial striae and occludental margin toothed; tergum was beaked and the
apex tinged with pinkish (Fig. 4.11g–j). Carinolaterals have rudimentary parietes, because of a
narrow strip separating radii from ala (Fig. 4.11k, l).
Figure 4.11. Hard body of Euacasta dofleini a) right side view b) left side view c) lateral plates view
d) rostral view e) upper view f) lower view g) external view of scutum h) internal view of scutum i)
external view of tergum j) internal view of tergum k) external view of plates l) internal view of plates.
Scale bars: 1 mm (a–f); 0.25 mm (g–h); 0.50 mm (i–l).
We dissected the soft part of the barnacle to observe the mouthparts, six pairs of cirri,
and penis in detail. Cirrus I with rami unequal in length, posterior margin with serrulate and
plumose setae (Fig. 4.12a). Cirrus II with rami unequal in length, terminal segments of anterior
and posterior rami with bifid setae (Fig. 4.12b). Cirral armature of cirrus IV was well developed
with curved hooks on the frontal margin of the segments of the anterior rami and basipod
(Fig. 4.12c). Cirrus V and VI were long and slender with equal rami in length (Fig. 4.12d, e).
Penis was annulate, without a basidorsal point, and gradually tapering to the tip (Fig. 4.12f).
Maxillule has nine large cuspidate setae with upper and lower pairs were the largest; and
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cutting margin was straight, without a notch (Fig. 4.12g). Mandible with five teeth, the second
was bifid, inferior angle with three denticles and stout setae, lower margin bearing simple
setae (Fig. 4.12h). Labrum bilobed with deep, V-shaped notch, and three teeth on each crest
(Fig. 4.12i).
Figure 4.12. Soft body of Euacasta dofleini a) cirrus I b) cirrus II c) cirrus IV d) cirrus V e) cirrus VI f) penis
g) maxillule h) mandible i) labrum. Scale bars: 200 µm (a–b, d–f ); 100 µm (c, g–i).
Membranobalanus longirostrum (Hoek, 1913)
The sponge Agelas sp. found at Saparua Island was inhabited by barnacle
Membranobalanus longirostrum (Fig. 4.13a, b). In the micro-CT overview scan of Agelas sp.
we found 23 specimens of barnacle inside the sponge. We discovered only hard parts of the
shell can be seen through micro-CT scan (Fig. 4.13c, d).
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Figure 4.13. Membranobalanus longirostrum (Hoek, 1913) (MZB Cru Cir 187) and its host sponge
a) sponge of Agelas sp. b) Membranobalanus longirostrum in the host sponge c) orifice view of
Membranobalanus longirostrum d) rostral view of Membranobalanus longirostrum. Scale bars: 2 cm
(a–b,); 1 mm (c–d).
The shell of the barnacle was thin, very brittle, and smooth without calcareous
spines; orifice was large and irregularly toothed; the basis was membranous (Fig. 4.14a–e).
Furthermore, we disassemble the shell to observe each plate in detail. Scutum with horizontal
growth ridges, occludental margin toothed at the lower part, adductor ridge was absent (Fig.
4.14b, c). Tergum was beaked, with horizontal growth ridges; spur was moderately wide and
close to the basi-scutal angle (Fig. 4.14b, c). The carina and rostrum were bowed; rostrum
was the longest plate, with the basal part extending far beyond the basal margin of the other
compartments (Fig. 4.14d, e).
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165
Figure 4.14. Hard body of Membranobalanus longirostrum a) shell, lateral view b) external view of
scutum and tergum c) internal view of scutum and tergum d) internal view of plates e) external view of
plates. Scale bars: 1 mm (a–f); 0.25 mm (g–h); 0.50 mm (i–l).
We dissected the soft part of the barnacle to observe the mouthparts, six pairs of cirri,
and penis in detail. Cirrus I with outer ramus 80% of inner ramus in length, terminal segments
of anterior and posterior rami with bifid setae (Fig. 4.15a). Cirrus II rami slightly unequal in
length, with plumose and serrulate setae; the distal segment of posterior ramus with serrulate
setae (Fig. 4.15b). Cirrus III with equal rami in length (Fig. 4.15c). Rami of cirrus IV slightly
unequal in length, with triangular spine-like teeth, form transverse comb-like rows and were
disposed on both segments of the pedicel (Fig. 4.15d). Cirrus V and VI were long and slender
(Fig. 4.15e, f). Penis was very long, much longer than the cirri (Fig. 4.15g). Maxillule cutting
margin was straight without a notch, with 11 large cuspidate setae, the lower pairs were the
largest (Fig. 4.15h). Mandible with five teeth, the second was bifid, the third was small; inferior
angle with three denticles and stout setae, lower margin bearing simple setae (Fig. 4.15i).
Labrum with a wide notch but not very deep; the shape was bilobed with two teeth on each
crest (Fig. 4.15j).
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Figure 4.15. Soft body of Membranobalanus longirostrum a) cirrus I b) cirrus II c) cirrus III d) cirrus IV e)
cirrus V f) cirrus VI g) penis h) maxillule i) mandible j) labrum. Scale bars: 200 µm (a–g); 100 µm (h–j).
4.5 Discussion
The results from this morphological study using an innovative approach with micro-CT
scanning and 3D reconstruction supports the results from Anderson (1994) who found out that
epizoic balanoid adaptations are related to the problem of overgrowth and burial, a reason
why the barnacles have to maintain its orifice at the surface of the sponge host. In our study, all
barnacle specimens were facing the surface of their sponge hosts. Ilan et al. (1999) also stated
that a sponge-inhabiting barnacle settles on the location of the opening to avoid overgrowth.
They also found that barnacles Neoacasta laevigata had a clumped distribution on its host
sponge Carteriospongia foliascens. According to Lewis (1992), this clump distribution related
to their adaptation for reproduction, since they are sessile organism so the key factor in the
pattern distribution of barnacles on a substrate is the distance from the nearest neighbor.
Yu et al. (2020) observed that the arrangement of barnacles in sponges are not random,
but mostly accumulate on the inhalant side to make it easier for capturing food. Sponges are
important benthic suspension feeders and are believed to slow the water flow and raise the
water turbulence, resulting in an increased residence time of possible nutrient particles (Gili
Exploring sponge-barnacle using micro-CT scans
167
& Coma, 1998). Barnacle as epibionts that can take advantage of the slowed water currents
caused by sponges (Nagler et al., 2017). All the statements above might explain the reason
why in this study the majority of barnacles found were arranged in groups near the surface of
the host sponge.
In this study, barnacle Acastinae were found inhabiting sponge Spongia sp., Axynissa
sp., and Cynacyrella sp., which have also previously been reported from the studies of sponge
barnacle of America, Red Sea, Indonesia, and worldwide (Van Syoc & Winther, 1999; Ilan
et al., 1999; Wibowo et al., 2011; Sulistiono et al., 2014; Van Syoc et al., 2015). While for
Membranobalanus, according to van Syoc (1988) and Hosie et al. (2019) they were restricted
to inhabiting sponges species of the Clionaidae. Additionally in this study, we found the species
Membranobalanus longirostrum inhabiting the sponge Agelas sp. of the family Agelasidae.
By using micro-CT we can see the pattern of calcareous projections on the surface of the
shell wall by looking at the pattern of circular holes on the shell, as these holes are the site
of attachment of the spines. These calcareous projections or spines are believed to function
as an anchor into the surrounding sponge tissue which is well developed in species that
inhabit crumbly sponges, but are reduced among species hosted by elastic sponges (Kolbasov,
1993). These features also can be used to distinguish Acastinae from members of the genus
Membranobalanus (Van Syoc et al., 2015). Because of this important role, it may be that
the pattern of these circular holes could also be used as an important character for species
identification in future. This study also confirms the advantage of using micro-CT imaging when
we examined Acasta sp. 2 which was anchored so tightly in the sponge tissue that removing
the specimen turned out to be impossible without breaking it.
Invertebrate studies utilising micro-CT scanning provide new ways of looking at the
external and internal morphology of organisms of microscopic size (Friedrich & Beutel,
2008). Although micro-CT scanning has great potential in biodiversity research of barnacles
by enhancing the visualization of external and internal anatomy, this study also had some
methodological limitations. In Membranobalanus longirostrum, the contrast in the 3D images
was too low to see the membranous basis. This problem could have been caused because
the membrane was tightly embedded in the sponge tissue, resulting in a low level of contrast
between the membrane and the surrounding host tissue. Although we applied a staining
method to visualize the membrane and soft body parts, the contrast of the images was
generally too low and we could not fully reconstruct the soft body parts. According to Metscher
(2009a), by using a few simple contrast stains, micro-CT can provide versatile, high-contrast,
quantitative 3D images of animal soft tissues. The methods can be used on a wide variety of
animal specimens fixed and preserved by the most common methods. The most broadly used
contrast stains tested so far are inorganic iodine and phosphotungstic acid (PTA) (Metscher,
2009b). These staining methods including iodine-based dyes such as Lugol’s solution poses a
simple, cost-effective, and nontoxic option for contrast enhancement for soft tissues (Heimel
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et al., 2019). The use of this staining method has a broad range of different concentrations of
iodine and staining durations depends on the type of tissue (Gignac et al., 2016). However,
marine organisms exhibit a large diversity of tissue types, chemical components and material
combinations, making it almost impossible to predict the effect of a certain contrast-enhancing
technique (Faulwetter et al., 2013). In a future project we plan to find a better staining agent
to find the most appropriate staining method to get a higher and sufficinet resolution of the
barnacles’ soft body.
In conclusion, this study demonstrates that micro-CT scanning is a very useful modern
technique on morphological and taxonomical examination of the sponge-inhabiting barnacles.
4.6 Acknowledgements
The first author would like to thank Prof. Frank Riedel from the Freie Universität Berlin for
his general support, Dr. André Beerlink (Xylon Hamburg) for his cooperation and introduction
of Xylon Hamburg and Tri Aryono Hadi (LIPI) for identifying the host sponges. Daniel Josep
Tala (LIPI) supported the sampling of barnacles in Indonesia. Bani Wahyudi and Abdul Basit
helped to improve the illustrations. Thanks to Kristin Mahlow and Prof. Johannes Müller for
supporting the micro-CT work progress at the Museum für Naturkunde Berlin.
This study was funded by the Ministry of Research, Technology and Higher Education,
the Republic of Indonesia within the Program for Research and Innovation in Science and
Technology (RISET-Pro), World Bank Loan No. 8245-ID. Barnacle samples were collected by the
first author with permit numbers as follows: 737/IPK.3/AP/IX/2016 for field work at Saparua
Island dan B-671/IPK.3/KP.01.06/IX72017 for field work at South Sulawesi.
Exploring sponge-barnacle using micro-CT scans
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4.7 Non-invasive 3D visualization of the sponge-inhabiting
barnacle Acasta sulcata (Crustacea: Cirripedia:
Balanomorpha) from the Moluccas, Indonesia. [Dataset].
Pipit Pitriana1,2,3, Kristina von Rintelen1, Andreas Wessel1
Museum für Naturkunde – Leibniz Institute for Evolution and Biodiversity Science,
Invalidenstrasse 43, 10115 Berlin, Germany
2
Research Centre for Deep-sea, Indonesian Institute of Science (LIPI), Jl. Y. Syaranamual, Poka,
Tlk. Ambon, Kota Ambon, Maluku, Indonesia
3
Institute of Geological Sciences, Freie Universität Berlin, Malteserstrasse 74–100 Building C
and D, 12249 Berlin, Germany
___________________________________________________________________
1
Published in: data publisher of Museum für Naturkunde Berlin (MfN) - Leibniz Institute for
Evolution and Biodiversity Science. The final authenticated version is available online at:
https://doi.org/10.7479/87tp-gr35.
___________________________________________________________________
Abstract
We present a digital reconstruction (video) of non-invasive microCT scans of barnacle
specimens (Acasta sulcata) embedded in their sponge host (Spongia sp.) from the Indonesian
island Saparua, Moluccas.
The sponge specimen, which was supposed to host barnacles, was subjected to microtomographic analysis at the Museum für Naturkunde Berlin, using a Phoenix nanotom X-rays
tube at 90 kV and 150 µA, generating 1440 projections. The specimen was fixed with foam
in a sealed plastic tube in an ethanol-saturated atmosphere. Cone beam reconstruction was
performed using the phoenix/x-ray datos/x version 2.3.3 software (GE Sensing and Inspection
Technologies GmbH). Effective voxel size, i.e. resolution in three-dimensional space, is 13.33
µm.
Data were visualized in VG Studio Max, version 3.1. Barnacles are shown as an isosurface
in red color, the surrounding sponge tissue as volume rendering in grey color (length of sponge
host approx. 11 cm; size of barnacles approx. 2.5 mm).
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Acknowledgements
This study was funded by the Ministry of Research, Technology and Higher Education,
the Republic of Indonesia within the Program for Research and Innovation in Science and
Technology (RISET-Pro), World Bank Loan No. 8245-ID. We thank Kristin Mahlow and Johannes
Müller for their support at the MfN Berlin and Prof. Frank Riedel (FU Berlin) for his general
support.
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General conclusion and outlook
5.1 General conclusion
This doctoral thesis was composed of three major works with four questions. The
first question dealt with the number of species of Moluccan barnacles collect from recent
fieldwork, including the species known to science, the number of new records, and the
number of new species. These questions are adressed in manuscripts 1 and 3, which deal
with recent field sampling activities. The resulting checklist of barnacles (Crustacea, Cirripedia)
from the Moluccas, East Indonesia, in the first manuscript lists twenty-five species of barnacles
collected from recent fieldwork, including twenty-three new records in the Moluccas and two
that are still awaiting description. Meanwhile, the checklist in manuscript 3 lists four barnacles
from Saparua Island, including one that is proposed as a new species.
The second quenstion is how many cryptic species are hidden in the collected material
of morphologically similar, can be answered with checklist of biodiversity discovery of
barnacles (Crustacea, Cirripedia) from the Moluccas, East Indonesia. Molecular analysis was
conducted to confirm the molecular taxonomic identity of the barnacles from the Moluccas
with sequences published in GenBank to examine whether they cluster near congeneric or
conspecific accessions. In total, 120 new sequences (COI = 62 sequences, 18S = 58 sequences)
were produced and a total of 172 sequences from GenBank (COI = 84 sequences, 18S = 88
sequences) were included in our analysis. The vast majority of new samples from the Moluccas
matched sequences from the same species that were available in GenBank. Two taxa for
which we have several sequences did not come up with a name in the BOLD system. The
isolated position in our tree leads us to the hypothesis that these taxa are new to science, i.e.
Microeuraphia sp. and Amphibalanus sp.
The third question, regarding the deep-sea barnacles collected from the Moluccas by
the Karubar expedition (1991), is discussed in manuscript 2. This collection is housed in the
Muséum National d’Histoire Naturelle, Paris and revealed 40 species recorded from the Kei, Aru
and Tanimbar Islands, and two of which we propose as new species. Based on comparison with
the literature on barnacles from Kei, Aru and Tanimbar Islands, 23 species can be considered
new records for these islands. Together with the checklists of all species in manuscript 1 and
sponge-dwelling barnacles in manuscript 3, this summary shows that 126 species are recorded
in the Moluccas, including 46 new records and 5 proposed new species.
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The fourth question, relating to the study of sponge-dwelling barnacles of the Moluccas
using a modern non-destructive imaging method, X-ray micro-computed tomography (MicroCT scanning), is discussed in manuscript 3. Through the use of micro-CT scanning, it can be
seen that barnacles generally live in groups near the surface of the host sponge. Using a microCT scan, the pattern of calcareous projections on the surface of the shell wall can also be seen
by looking at the pattern of circular holes, as these holes are the sites of spinal attachment.
This study also confirms the advantage of using micro-CT scan for the examination of sponge
barnacles, whose deep roots in sponge tissues make it almost impossible to pull out barnacles
without breaking them.
Based on these results, this doctoral thesis concludes that 126 species are recorded
from the Moluccas, including 46 new records, and 5 proposed new species. This supports the
assumption that the Indo-Malayan region (including the Moluccas Islands) is a centre of high
benthic biodiversity. However, a comparison of the number of species previously recorded
from the Moluccas with those recorded in this study indicates that species diversity for each
island has been heavily underestimated. Molecular results also indicate that the barnacle fauna
of the region is understudied. Nonetheless, a modern non-destructive imaging method, X-ray
micro-computed tomography (Micro-CT scanning) is proven to be a very useful technique for
studying the morphology and taxonomy of barnacles from the Moluccas. When paired with
classic morphological examination, this technique has the potential to be an extremely useful
tool for understanding the integrative taxonomy of barnacles and helping identify further
species of barnacles.
5.2 Outlook
This doctoral thesis has demonstrated that, despite being part of the global epicentre
of marine biodiversity, knowledge of the barnacle fauna of the Moluccas is still limited. For
example, seven species were previously known from around Ambon Island, compared to the
twenty-four species found in this study. For smaller islands, such as Saparua and Pombo, no
barnacle species were previously recorded. Given the size of the Moluccan Archipelago, which
consists of more than 1,000 islands—many of which, including relatively large islands such as
Haruku, Buru, Yamdena or Wetar, have never been sampled—it can be expected that more
intensive sampling of these islands would yield a much higher number of species.
The molecular results also indicate that the barnacles of the region are understudied.
The generic assignment of some described species is also challenged. For example,
Amphibalanus zhujiangensis was found to be more closely related to Megabalanus than to
other Amphibalanus species, suggesting the need to conduct in-depth research on this species
to clarify its taxonomy. These points all underline again the necessity of a more comprehensive
approach to sampling in the region, as well as the need to explore more molecular markers for
a truly integrative taxonomy of barnacles, not just in the Moluccas.
General conclusion and outlook
Considering that the Moluccas have long been a destination for scientific exploration,
having hosted at least fifty scientific expeditions, it is challenging to obtain the specimen data
that is deposited in museums around the world. In addition, for most genera of deep-sea
barnacles, taxonomic information is limited and reliable taxonomic keys are rare. Many species
of barnacles were described based on a single (adult) specimen. Data on morphological
changes during larval and post-larval development are mostly lacking. Limited sample sizes, for
example from previous research expeditions, do not allow for the assessment of intraspecific
variability. The only chance to contribute more taxonomic data on barnacles from such remote
areas as the Moluccas is to re-examine the type and other specimens deposited in museum
collections. These reasons demonstrate once more the importance of museum collections as
a study resource in biodiversity science.
The deep-sea barnacle specimens from the Moluccas deposited in museum collections
could be compared with recently collected specimens. Although it is difficult to collect or
examine evidence from deep-sea areas, and although these areas seem so far away that it
is unimaginable that human activity would affect them, deep-sea barnacles might provide
clues how climate change is affecting the deep ocean. They may also provide data regarding
whether environmental changes such as global warming could potentially affect the deep
oceans, which remain among the most mysterious parts of the world.
Barnacle studies utilising micro-CT scanning provide us new ways of looking at the
external and internal morphology of organisms in the millimetre–micrometre size range.
However, in this study we experienced a problem with unsatisfactorily low-contrast image
of soft body. Even after staining, soft body details could not be seen clearly. As such, various
methods must be attempted to determine the staining method that is best suited to examining
soft body through micro-CT scanning.
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193
CHAPTER 7
APPENDIX
7. 1 Supplementary material for An annotated checklist and
integrative biodiversity discovery of barnacles (Crustacea:
Cirripedia) from the Moluccas, East Indonesia
Table 7.1. Table of sample information for DNA samples sequenced for this study.
No.
Species name
Island
Internal code
Specimen voucher
1
Amphibalanus
zhujiangensis
A. zhujiangensis
A. zhujiangensis
A. zhujiangensis
A. zhujiangensis
A. zhujiangensis
A. zhujiangensis
A. zhujiangensis
Amphibalanus
variegatus
A. variegatus
A. variegatus
A. variegatus
A. variegatus
Amphibalanus
amphitrite
A. amphitrite
Amphibalanus sp.
A. sp.
A. sp.
A. sp.
A. sp.
A. sp.
Acasta sp.
Tetraclita
singaporensis
T. singaporensis
T. singaporensis
T. singaporensis
Saparua
Bcl1716PM775
MZB Cru Cir 019-1
GenBank number
COI
18S RNA
MK995334 MK981347
Sulawesi
Saparua
Seram
Sulawesi
Ambon
Sulawesi
Sulawesi
Saparua
Bcl4517PM1404
Bcl9016PM1222
Bcl3317PM1307
Bcl4517PM1406
Bcl2316PM803
Bcl4517PM1405
Bcl4517PM1407
Bcl5316PM1004
Bcl4517PM1404
MZB Cru Cir 021
MZB Cru Cir 010-3
Bcl4517PM1406
MZB Cru Cir 016-1
Bcl 4517PM1405
Bcl4517PM1407
MZB Cru Cir 013-1
MK995335
MK995336
MK995337
MK995338
MK995339
MK995340
MK995341
MK995342
Saparua
X3PM922
Saparua
X2PM732
Saparua Bcl4416PM953
Sulawesi Bcl7717PM1874
Sulawesi Bcl6317PM1730
MZB Cru Cir 015-2
MZB Cru Cir 015-1
MZB Cru Cir 013-2
Bcl7717PM1874
Bcl6317PM1730
MK995343
MK995344
MK995345 MK981355
MK995346
MK995347
-
Sulawesi
Ambon
Seram
Ambon
Sulawesi
Seram
Sulawesi
Sulawesi
Sulawesi
Bcl6317PM1780
MZB Cru Cir 136
MZB Cru Cir 137-2
MZB Cru Cir 135
Bcl5217PM1550
MZB Cru Cir 137-1
Bcl7517PM1857
Y0117PM1750
Bcl5417PM1640
MK995348
MK995349
MK995350
MK995351
MK995352
MK995353
MK995354
MK995355
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
Bcl6317PM1780
Bcl1117PM125
Bcl4117PM1379
Bcl4216PM937
Bcl5217PM1550
Bcl4117PM1378
Bcl7517PM1857
Y0117PM1750
Bcl5417PM1640
Sulawesi Bcl5717PM1663
Sulawesi Bcl5917PM1699
Sulawesi Bcl7617PM1867
Bcl5717PM1663
Bcl5917PM1699
Bcl7617PM1867
MK981348
MK981349
MK981350
MK981351
MK981352
MK981353
MK981354
-
MK981356
MK981357
MK981358
MK981359
MK981360
MK981361
MK981362
MK981363
MK995356 MK981364
MK995357 MK981365
MK995358 MK981366
Chapter 7
194
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
T. singaporensis
Tetraclita
squamosa
T. squamosa
T. squamosa
T. squamosa
T. squamosa
T. squamosa
T. squamosa
Tetraclita
kuroshioensis
T. kuroshioensis
T. kuroshioensis
T. kuroshioensis
T. kuroshioensis
Newmanella
spinosus
Yamaguchiella
coerulescens
Neonrosella
vitiata
Tesseropora rosea
Dosima
fascicularis
Heteralepas
japonica
Lepas anserifera
L. anserifera
L. anserifera
Nesochthamalus
intertextus
Capitulum mitella
Chthamalus moro
C. moro
C. moro
C. moro
C. moro
C. moro
C. moro
C. moro
C. moro
C. moro
C. moro
C. moro
Sulawesi Bcl7417PM1847
Saparua Bcl8416PM1199
Bcl7417PM1847
MZB Cru Cir 093-1
MK995359 MK981367
MK995360 MK981368
Ambon
Bcl1517PM291
Saparua Bcl4916PM986
Ambon
Bcl1717PM347
Ambon
Bcl0917PM120
Ambon
Bcl3017PM720
Sulawesi Bcl6517PM1769
Ambon
Bcl0317PM13
MZB Cru Cir 081-1
MZB Cru Cir 095-1
MZB Cru Cir 096-1
MZB Cru Cir 092-1
MZB Cru Cir 099-1
Bcl6517PM1769
MZB Cru Cir 097
MK995361
MK995362
MK995363
MK981369
MK981370
MK981371
MK981372
MK981373
MK981374
MK981375
Ambon
Sulawesi
Sulawesi
Saparua
Sulawesi
Bcl0717PM39
Bcl6417PM1740
Bcl4417PM1403
Bcl7916PM1180
Bcl4817PM1441
MZB Cru Cir 098-1
Bcl6417PM1740
Bcl4417PM1403
MZB Cru Cir 100
Bcl4817PM1441
MK995364
MK995365
MK995366
MK995367
MK995368
MK981376
MK981377
MK981378
MK981379
-
Ambon
Bcl1216PM494
MZB Cru Cir 126-1
-
MK981381
Ambon
X1PM723
MZB Cru Cir 132-1
-
MK981384
Ambon
Ambon
Bcl0216PM27
Bcl2417PM500
MZB Cru Cir 075-1
MZB Cru Cir 048-1
MK995370
MK995371 MK981385
Deep
sea
Ambon
Seram
Seram
Ambon
Bcl9416PM1911
MZB Cru Cir 050-1
MK995372 MK981386
Bcl2317PM495
Bcl3417PM1322
Bcl3517PM1323
Bcl2217PM429
MZB Cru Cir 059-1
MZB Cru Cir 061-1
MZB Cru Cir 062-1
MZB Cru Cir 070-1
MK995373
MK995374 MK981387
MK995375 MK981388
MK995376 MK981389
Ambon
Ambon
Saparua
Saparua
Ambon
Pombo
Seram
Ambon
Saparua
Pombo
Saparua
Ambon
Seram
Bcl0617PM34
Bcl0417PM14
Bcl4116PM902
Bcl3316PM872
Bcl1417PM289
Bcl8816PM1217
Bcl4317PM1388
Bcl1217PM243
Bcl5916PM1052
Bcl8816PM1218
Bcl2116PM787
Bcl2717PM613
Bcl4317PM1389
MZB Cru Cir 032-1
MZB Cru Cir 039-1
MZB Cru Cir 046-1
MZB Cru Cir 045-1
MZB Cru Cir 036-1
MZB Cru Cir 043-1
MZB Cru Cir 047-1
MZB Cru Cir 040-1
MZB Cru Cir 044-2
MZB Cru Cir 043-2
MZB Cru Cir 044-1
MZB Cru Cir 041-1
MZB Cru Cir 047-2
MK995377
MK995378
MK995379
MK995380
MK995381
MK995382
MK995383
MK995384
MK995385
MK995386
MK995387
MK995388
MK981390
MK981391
MK981392
MK981393
MK981394
MK981395
MK981396
MK981397
MK981398
MK981399
MK981400
Appendix
63
64
65
66
67
68
69
70
Microeuraphia sp.
M. sp.
M. sp.
M. sp.
M. sp.
M. sp.
M. sp.
M. sp.
Seram
Seram
Sulawesi
Sulawesi
Sulawesi
Sulawesi
Sulawesi
Sulawesi
Bcl3917PM1358
Bcl3917PM1359
Bcl4917PM1510
Bcl5517PM1641
X4PM1494
Bcl5317PM1625
Bcl6217PM1725
X5PM1581
MZB Cru Cir 138-1
MZB Cru Cir 138-2
Bcl4917PM1510
Bcl5517PM1641
X4PM1494
Bcl5317PM1625
Bcl6217PM1725
X5PM1581
MK995389
MK995390
MK995391
MK995392
MK995393
MK995394
MK995395
MK995396
195
MK981401
MK981402
MK981403
MK981404
MK981405
MK981406
MK981407
Table 7.2. Measurements for Heteralepas japonica (N = 25).
Parameter
A
B
C
D
E
F
G
A/B
A/C
A/D
A/E
A/F
A/G
B/C
B/D
B/E
B/F
B/G
C/D
C/E
C/F
C/G
D/E
D/F
D/G
E/F
E/G
F/G
Range (mm)
11.9-18.6
8.7-15.3
7.7-27.6
3.2-9.4
2.0-3.0
7.0-13.3
5.1-10.4
1.1-1.5
0.6-1.7
1.6-3.9
4.1-9.2
1.4-2.1
1.6-2.5
0.5-1.5
1.4-3.6
2.9-6.9
1.1-1.8
1.3-2.1
1.3-4.8
3.8-12.5
0.9-3.1
1.3-3.9
1.7-4.1
0.4-1.1
0.6-1.3
0.2-0.4
0.2-0.5
0.9-1.8
Mean ± SD (mm)
15.1 ± 0.9
12.2 ± 2.5
16.3 ± 2.7
6.5 ± 0.8
2.5 ± 0.0
9.1 ± 0.2
7.3 ± 1.0
1.2 ± 0.2
1.0 ± 0.4
2.5 ± 0.8
6.3 ± 0.5
1.7 ± 0.1
2.1 ± 0.4
0.7 ± 0.5
2.0 ± 1.1
5.1 ± 1.2
1.4 ± 0.3
1.7 ± 0.6
2.7 ± 0.2
6.5 ± 1.3
1.8 ± 0.4
2.2 ± 0.2
2.7 ± 0.4
0.7 ± 0.1
0.9 ± 0.0
0.3 ± 0.0
0.4 ± 0.0
1.3 ± 0.2
Median (mm)
14.5
12.2
11.1
4.4
2.0
7.6
7.2
1.2
1.4
3.3
7.2
1.9
2.0
1.2
2.8
6.1
1.6
1.7
2.5
5.5
1.5
1.5
2.2
0.6
0.6
0.3
0.3
1.1
Key to parameters: A = capitulum height; B = capitulum width; C = peduncle length; D = orifice
height; E = number of crests; F = capitulum thickness; G = peduncle width.
196
Chapter 7
Table 7.3. Measurements for Dosima fascicularis (N = 6).
Parameter
TH
CH
DBC
CS
LS
WS
LT
WT
TH/CH
TH/DBC
TH/CS
TH/LS
TH/WS
TH/LT
TH/WT
CH/DBC
CH/CS
CH/LS
CH/WS
CH/LT
CH/WT
DBC/CS
DBC/LS
DBC/WS
DBC/LT
DBC/WT
CS/LS
CS/WS
CS/LT
CS/WT
LS/WS
LS/LT
LS/WT
WS/LT
WS/WT
LT/WT
Range (mm)
11.7-19.5
10.5-17.1
4.0-6.8
6.5-10.2
8.1-11.1
5.4-7.3
6.2-11.1
2.7-4.4
1.1-1.2
2.6-3.2
1.8-2.2
1.4-1.9
2.2-2.6
1.7-2.1
2.9-5.7
2.1-2.7
1.6-1.7
1.3-1.5
1.9-2.1
1.5-1.7
2.6-4.2
0.6-0.8
0.5-0.7
0.7-0.9
0.6-0.7
1.0-2.0
0.8-0.9
1.2-1.2
0.9-1.1
1.6-2.7
1.3-1.5
1.0-1.3
2.1-3.1
0.7-0.9
1.3-2.3
1.5-2.8
Mean ± SD (mm)
16.0 ± 0.8
13.7 ± 2.5
5.5 ± 0.2
8.3 ± 1.2
9.6 ± 0.8
6.9 ± 1.0
8.6 ± 1.6
3.6 ± 0.8
1.2 ± 0.2
2.9 ± 0.0
1.9 ± 0.2
1.7 ± 0.1
2.3 ± 0.2
1.9 ± 0.2
4.5 ± 0.9
2.5 ± 0.3
1.6 ± 0.2
1.4 ± 0.1
2.0 ± 0.1
1.6 ± 0.0
3.8 ± 0.2
0.7 ± 0.1
0.6 ± 0.0
0.8 ± 0.1
0.6 ± 0.1
1.5 ± 0.3
0.9 ± 0.0
1.2 ± 0.0
1.0 ± 0.0
2.3 ± 0.2
1.4 ± 0.1
1.1 ± 0.1
2.7 ± 0.4
0.8 ± 0.0
1.9 ± 0.2
2.4 ± 0.2
Median (mm)
19.0
15.3
6.6
9.4
10.6
8.0
10.0
3.8
1.3
2.9
2.0
1.8
2.9
1.9
5.1
2.3
1.6
1.5
1.9
1.5
4.1
0.7
0.6
0.8
0.7
1.8
0.9
1.2
0.9
2.5
1.3
1.1
2.8
0.8
2.1
2.6
Key to parameters: TH = total height; CH = capitulum height; DBC = diameter of the base of
capitulum; LS = scutum length; WS = scutum width; LT = tergum length; WT = tergum width.
Appendix
197
Table 7.4. Measurements for Lepas anserifera (N = 25).
Parameter
TH
CH
DBC
CS
LS
WS
LT
WT
TH/CH
TH/DBC
TH/CS
TH/LS
TH/WS
TH/LT
TH/WT
CH/DBC
CH/CS
CH/LS
CH/WS
CH/LT
CH/WT
DBC/CS
DBC/LS
DBC/WS
DBC/LT
DBC/WT
CS/LS
CS/WS
CS/LT
CS/WT
LS/WS
LS/LT
LS/WT
WS/LT
WS/WT
LT/WT
Range (mm)
14.2-31.9
8.1-14.8
2.0-7.2
5.2-12.4
6.3-12.3
4.7-11.3
4.3-9.6
1.6-3.2
1.3-2.5
2.7-7.2
1.3-3.8
1.3-3.1
1.5-4.1
2.1-5.5
5.8-13.8
1.7-4.7
1.0-1.7
1.0-1.4
1.1-2.0
1.3-2.2
3.9-5.9
0.3-0.8
0.3-0.7
0.4-0.9
0.3-0.9
0.9-3.3
0.7-1.0
1.0-1.2
0.9-1.8
2.4-5.5
1.1-1.8
1.1-2.0
3.6-5.6
0.8-1.6
2.1-5.1
2.4-4.3
Mean ± SD (mm)
21.1 ± 4.0
12.0 ± 1.3
4.0 ± 0.6
8.1 ± 1.5
9.6 ± 1.3
7.3 ± 1.2
7.7 ± 0.6
2.5 ± 0.2
1.8 ± 0.1
5.5 ± 0.2
2.6 ± 0.0
2.2 ± 0.1
2.9 ± 0.0
2.8 ± 0.3
8.5 ± 1.0
3.2 ± 0.1
1.5 ± 0.1
1.3 ± 0.1
1.7 ± 0.1
1.6 ± 0.0
4.8 ± 0.2
0.5 ± 0.0
0.4 ± 0.0
0.6 ± 0.0
0.5 ± 0.0
1.6 ± 0.1
0.9 ± 0.0
1.1 ± 0.0
1.1 ± 0.1
3.3 ± 0.4
1.3 ± 0.0
1.3 ± 0.1
3.9 ± 0.3
1.0 ± 0.1
2.9 ± 0.3
3.1 ± 0.1
Median (mm)
22.2
13.0
4.0
8.5
9.6
7.7
7.9
2.7
1.7
5.6
2.6
2.3
2.9
2.8
8.1
3.3
1.5
1.4
1.7
1.7
4.7
0.5
0.4
0.5
0.5
1.4
0.9
1.1
1.1
3.1
1.3
1.2
3.5
1.0
2.8
2.9
Key to parameters: TH = total height; CH = capitulum height; DBC = diameter of the base of
capitulum; LS = scutum length; WS = scutum width; LT = tergum length; WT = tergum width.
198
Chapter 7
Table 7.5. Measurements for Capitulum mitella (N = 25).
Parameter
TH
CH
RC
RT
DBC
TH/CH
TH/RC
TH/RT
TH/DBC
CH/RC
CH/RT
CH/DBC
RC/RT
RC/DBC
RT/DBC
Range (mm)
10.7-7.1
7.0- 4.6
5.8-27.3
5.5-24.2
4.5-20.4
1.2-2.2
1.0-2.2
1.3-2.5
1.5-2.9
0.7-1.2
0.8-1.4
0.9-1.9
0.9-1.3
1.3-1.7
1.0-1.5
Mean ± SD (mm)
24.7 ± 10.7
14.9 ± 5.4
15.6 ± 6.3
13.7 ± 5.7
11.0 ± 10.8
1.6 ± 1.6
1.6 ± 0.3
1.8 ± 0.3
2.3 ± 0.4
1.0 ± 0.1
1.1 ± 0.1
1.4 ± 1.4
1.2 ± 1.2
1.4 ± 0.1
1.3 ± 0.1
Median (mm)
26.7
16.6
17.1
15.2
10.6
1.6
1.6
1.8
2.3
1.0
1.1
1.4
1.2
1.4
1.2
Key to parameters: TH = total height; CH = capitulum height; RC = distance from rostrum to
carina; RT = rostrum height; DBC = capitulum diameter.
Table 7.6. Measurements for Pseudoctomeris sulcata (N = 2).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
16.2-16.7
5.5-7.9
7.4-7.7
4.3-6.1
11.5-15.0
2.0-3.1
2.1-2.2
2.7-3.9
1.1-1.5
0.7-1.0
1.3-1.3
0.5-0.5
0.6-0.8
1.5-1.9
2.5-2.7
Mean ± SD (mm)
16.4 ± 0.3
6.7 ± 1.8
7.6 ± 0.2
5.2 ± 1.3
13.3 ± 2.5
2.5 ± 0.7
2.2 ± 0.1
3.3 ± 0.9
1.3 ± 0.3
0.9 ± 0.2
1.3 ± 0.0
0.5 ± 0.0
0.7 ± 0.2
1.8 ± 0.3
2.6 ± 0.2
Median (mm)
16.4
6.7
7.6
5.2
13.3
2.6
2.2
3.3
1.3
0.9
1.3
0.5
0.7
1.7
2.6
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Appendix
199
Table 7.7. Measurements for Hexechamaesipho pilsbryi (N = 10).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
8.9-17.0
4.2-6.9
1.0-3.7
3.6-5.6
10.3-16.4
1.7-4.5
0.8-1.3
1.4-6.6
0.8-1.4
1.4-6.6
1.0-1.7
0.3-0.5
1.4-3.9
3.1-16.6
2.2-4.3
Mean ± SD (mm)
14.1 ± 3.4
5.7 ± 0.5
1.7 ± 0.1
4.3 ± 0.0
13.4 ± 0.3
2.3 ± 0.4
8.4 ± 2.6
3.3 ± 0.9
1.1 ± 0.3
3.4 ± 0.5
1.3 ± 0.1
0.4 ± 0.1
2.6 ± 0.2
8.0 ± 0.3
3.1 ± 0.1
Median (mm)
14.6
5.4
1.7
3.7
12.6
2.7
8.7
3.9
1.2
3.2
1.5
0.4
2.2
7.4
3.4
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Table 7.8. Measurements for Nesochthamalus intertextus (N = 10).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
8.9-12.1
3.0-4.9
1.3-3.1
2.5-3.9
6.9-10.3
2.2-3.5
3.5-8.1
2.8-3.9
0.9-1.4
1.3-2.3
1.0-1.4
0.4-0.6
1.2-2.1
2.6-6.0
2.2-3.8
Mean ± SD (mm)
10.5 ± 0.2
3.8 ± 0.5
2.2 ± 0.3
3.3 ± 0.3
9.0 ± 0.4
2.8 ± 0.3
5.0 ± 0.6
3.2 ± 0.3
1.2 ± 0.1
1.8 ± 0.0
1.2 ± 0.2
0.4 ± 0.1
1.5 ± 0.4
4.3 ± 0.8
2.8 ± 0.1
Median (mm)
11.0
4.6
2.2
3.7
10.0
2.4
5.1
3.0
1.1
2.1
1.2
0.5
1.7
4.7
2.7
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
200
Chapter 7
Table 7.9. Measurements for Euraphia hembeli (N = 1).
Parameter
Value (mm)
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
37.4
18.3
14.3
15.2
28.6
2.1
2.6
2.5
1.3
1.3
1.2
0.6
1.1
2.0
1.9
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Table 7.10. Measurements for Microeuraphia sp. (N = 2).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
3.6-9.9
1.5-4.5
1.2-2.2
0.7-3.6
3.0-9.1
2.2-2.5
3.1-4.3
2.8-5.0
1.1-1.2
1.2-2.0
1.3-2.0
0.4-0.5
0.6-1.6
2.6-4.0
2.1-4.0
Mean ± SD (mm)
6.8 ± 4.4
3.0 ± 2.2
1.7 ± 0.8
2.1 ± 2.0
6.0 ± 4.3
2.3 ± 0.2
3.7 ± 0.9
3.9 ± 1.5
1.1 ±0.1
1.6 ± 0.5
1.6 ± 0.5
0.5 ± 0.0
1.1 ± 0.7
3.3 ± 1.0
3.3 ± 1.2
Median (mm)
6.8
3.0
1.7
2.1
6.0
2.3
3.7
3.9
1.1
1.6
1.6
0.5
1.1
3.3
3.3
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Appendix
201
Table 7.11. Measurements for Chthamalus moro (N = 25).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
2.4-5.1
1.0-3.4
0.8-1.7
0.7-1.7
1.4-4.1
1.4-3.3
2.0-4.4
1.7-4.3
1.1-1.7
0.8-2.4
0.8-2.9
0.4-1.2
0.6-1.4
1.2-3.2
1.0-3.5
Mean ± SD (mm)
3.6 ± 0.8
1.7 ± 1.5
1.3 ± 0.0
1.2 ± 0.1
2.8 ± 0.5
2.3 ± 1.0
2.9 ± 0.5
3.2 ± 0.4
1.3 ± 0.0
1.4 ± 0.9
1.5 ± 1.1
0.6 ± 0.3
0.9 ± 0.1
2.3 ± 0.3
2.5 ± 0.2
Median (mm)
4.5
2.4
1.7
1.1
3.7
2.2
2.7
4.1
1.2
1.4
2.1
0.6
0.7
2.2
3.4
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Table 7.12. Measurements for Tetraclitella divisa ( N = 1).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Value (mm)
11.1
4.9
2.4
3.5
9.7
2.3
4.7
3.1
1.2
2.1
1.4
0.5
1.5
4.1
2.7
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
202
Chapter 7
Table 7.13. Measurements for Tetraclitella karandei (N = 3).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
10.1-17.4
3.8-5.6
0.4-0.7
2.7-5.2
8.2-18.2
2.7-3.6
13.6-48.3
3.3-4.3
1.0-1.2
5.1-15.4
1.1-1.4
0.3-0.5
3.7-14.5
11.1-50.5
3.0-3.9
Mean ± SD (mm)
14.2 ± 3.7
4.5 ± 3.7
0.6 ± 0.1
3.8 ± 0.6
13.4 ± 4.0
3.1 ± 0.7
29.3 ± 8.7
3.8 ± 0.4
1.1 ± 0.1
9.2 ± 1.5
1.2 ± 0.2
0.4 ± 0.1
8.1 ± 1.7
28.4 ± 8.8
3.5 ± 0.6
Median (mm)
12.7
4.0
0.7
3.2
11.1
3.1
19.7
4.0
1.2
6.2
1.3
0.4
4.9
17.3
3.5
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Table 7.14. Measurements for Tesseropora rosea ( N = 15).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
9.7-25.6
2.9-7.8
4.4-13.0
2.3-6.9
9.7-24.5
1.5-4.9
1.4-3.6
1.8-5.4
0.8-1.4
0.3-1.5
1.1-1.3
0.2-0.8
0.3-1.4
1.2-4.2
1.7-5.3
Mean ± SD (mm)
16.6 ± 7.5
6.1 ± 0.1
8.4 ± 2.8
5.3 ± 0.5
15.2 ± 10.5
2.8 ± 1.2
2.0 ± 0.2
3.3 ± 1.9
1.1 ± 0.3
0.8 ± 0.2
1.2 ± 0.2
0.4 ± 0.3
0.7 ± 0.3
1.9 ± 0.6
3.0 ± 2.5
Median (mm)
19.1
6.0
9.0
5.0
17.1
3.2
2.1
3.9
1.2
0.7
1.2
0.4
0.6
1.8
3.5
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Appendix
203
Table 7.15. Measurements for Tetraclita kuroshioensis (N = 5).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
12.1-21.6
3.2-5.3
7.3-10.4
2.4-4.2
18.1-21.8
3.3-5.4
1.4-2.8
4.4-8.4
0.8-1.1
0.4- 0.5
1.2-1.6
0.2-0.3
0.3-0.4
1.3-2.5
3.9-7.5
Mean ± SD (mm)
17.2 ± 5.8
3.9 ± 0.2
8.4 ± 1.1
2.9 ± 0.3
17.7 ± 5.0
4.5 ± 1.3
2.1 ± 1.0
6.0 ± 2.9
1.0 ± 0.0
0.5 ± 0.1
1.3 ± 0.2
0.2 ± 0.1
0.4 ± 0.0
2.1 ± 0.9
6.1 ± 2.5
Median (mm)
16.2
3.8
8.0
2.6
14.5
4.3
2.1
6.4
1.1
0.5
1.5
0.3
0.3
1.9
5.7
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Table 7.16. Measurements for Tetraclita squamosa (N = 5).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
20.1-30.2
4.4-7.9
11.9-14.2
3.4-7.2
19.3-28.3
3.8-4.6
1.6-2.4
4.2-5.9
0.9-1.2
0.4-0.6
1.0-1.3
0.2-0.3
0.3-0.5
1.6-2.1
3.9-5.7
Mean ± SD (mm)
25.4 ± 3.4
6.1 ± 1.7
12.9 ± 0.3
5.3 ± 1.4
24.8 ± 0.2
4.2 ± 0.6
2.0 ± 0.2
4.9 ± 0.6
1.0 ± 0.1
0.5 ± 0.1
1.2 ± 0.0
0.3 ± 0.1
0.4 ± 0.1
1.9 ± 0.0
4.9 ± 1.1
Median (mm)
27.6
6.7
12.5
5.7
26.0
4.2
2.2
4.9
1.1
0.5
1.2
0.3
0.5
2.1
4.7
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
204
Chapter 7
Table 7.17. Measurements for Yamaguchiella coerulescens (N = 25).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
8.3 – 29.0
4.2 – 11.5
5.7 – 17.7
3.2 – 11.8
8.5 – 27.8
1.8 – 3.5
1.4 – 2.7
1.9 – 3.7
0.8 – 1.3
0.5 – 1.0
0.9 – 1.3
0.3 – 0.5
0.5 – 0.9
1.4 – 2.9
2.1 – 3.8
Mean ± SD (mm)
19.5 ± 12.6
7.1 ± 3.3
11.0 ± 5.2
7.1 ± 4.1
19.7 ±12.0
2.8 ± 0.7
1.8 ±0.4
2.8 ± 0.5
0.1 ± 0.1
0.7 ± 0.1
1.0 ± 0.1
0.4 ± 0.1
0.7 ± 0.0
1.8 ± 0.2
2.8 ± 0.3
Median (mm)
17.2
6.8
9.3
7.4
18.6
2.3
1.7
2.2
0.9
0.8
0.9
0.4
0.8
1.9
2.4
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Table 7.18. Measurements for Neonrosella vitiata (N = 4).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
15.4-22.8
5.2-6.9
5.3-6.9
5.0-5.4
13.8-22.8
2.8-3.7
2.9-4.0
3.1-4.3
0.9-1.1
1.0-1.1
1.1-1.3
0.3-0.4
0.8-0.9
2.6-4.0
2.8-4.3
Mean ± SD (mm)
18.9 ± 5.3
5.9 ± 0.5
5.8 ± 0.3
5.1 ± 0.2
18.8 ± 6.3
3.2 ± 0.6
3.3 ± 0.8
3.7 ± 0.9
1.0 ± 0.1
1.0 ± 0.0
1.2 ± 0.0
0.3 ± 0.1
0.9 ± 0.0
3.2 ± 1.0
3.7 ± 1.1
Median (mm)
19.1
5.8
5.5
5.2
18.3
3.3
3.4
3.7
1.1
1.0
1.1
0.3
0.9
3.3
3.5
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Appendix
205
Table 7.19. Measurements for Newmanella spinosus (N = 5).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
17.4-21.0
5.3-7.2
6.8-8.9
5.0-6.7
15.9-20.5
2.7-3.3
2.0-2.8
2.9-3.6
1.0-1.1
0.6-0.9
1.0-1.2
0.3-0.4
0.6-0.8
1.8-2.9
2.9-3.7
Mean ± SD (mm)
19.3 ± 1.1
6.5 ± 0.7
8.1 ± 1.5
5.9 ± 0.2
18.8 ± 2.5
3.0 ± 0.2
2.4 ± 0.6
3.3 ± 0.1
1.0 ± 0.1
0.8 ± 0.2
1.1 ± 0.1
0.4 ± 0.0
0.7 ± 0.2
2.4 ± 0.8
3.2 ± 0.4
Median (mm)
18.2
5.8
7.8
5.1
17.6
3.2
2.4
3.5
1.0
0.8
1.1
0.3
0.7
2.3
3.4
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Table 7.20. Measurements for Amphibalanus amphitrite (N = 15).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
3.1-17.8
1.5-8.1
2.1-10.8
1.5-5.4
2.8-17.6
1.4-3.3
1.2-5.8
1.7-4.9
0.8-2.7
0.6-3.2
0.9-1.6
0.3-1.1
0.4-2.0
0.6-4.7
1.4-3.5
Mean ± SD (mm)
10.3 ± 1.2
5.1 ± 1.5
5.4 ± 0.4
3.8 ± 1.2
8.6 ± 1.6
2.0 ± 0.7
2.0 ± 0.1
2.2 ± 1.2
1.2 ± 0.2
1.0 ± 0.3
1.3 ± 0.1
0.6 ± 0.1
0.8 ± 0.2
1.7 ± 0.2
2.3 ± 0.5
Median (mm)
9.2
4.0
4.7
2.9
6.5
2.6
2.0
3.7
1.5
0.8
1.4
0.6
0.6
1.4
2.5
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
206
Chapter 7
Table 7.21. Measurements for Amphibalanus reticulatus (N = 5).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
7.7-16.3
3.5-8.9
3.2-10.5
2.5-7.1
2.9-15.5
1.5-2.5
1.3-3.2
1.8-3.5
0.9-4.9
0.8-1.4
1.1-1.9
0.5-3.0
0.5-1.0
0.3-2.5
0.4-3.0
Mean ± SD (mm)
12.1 ± 2.7
6.8 ± 1.2
6.9 ± 0.9
4.9 ± 0.8
10.0 ± 2.4
1.8 ± 0.1
1.8 ± 0.2
2.5 ± 0.1
1.4 ± 0.0
1.0 ± 0.0
1.4 ± 0.1
0.8 ± 0.0
0.7 ± 0.0
1.5 ± 0.2
2.1 ± 0.1
Median (mm)
10.7
5.7
5.2
3.5
9.6
1.9
2.0
3.1
1.1
1.1
1.7
0.7
0.7
1.8
2.7
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Table 7.22. Measurements for Amphibalanus variegatus (N = 10).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
Mean ± SD (mm) Median (mm)
8.3-11.8
10.3 ± 1.1
9.3
4.8-8.8
6.4 ± 0.4
5.2
4.3-8.4
5.7 ± 0.8
5.1
3.4-5.3
4.2 ± 0.3
3.9
6.9-10.4
9.1 ± 1.4
8.3
1.3-2.0
1.7 ± 0.1
1.8
1.4-2.1
1.9 ± 0.1
1.8
2.2-2.8
2.5 ± 0.1
2.4
1.0-1.2
1.1 ± 0.1
1.1
0.9-1.4
1.1 ± 0.1
1.0
1.3-1.8
1.5 ± 0.0
1.3
0.6-0.9
0.7 ± 0.1
0.6
0.6-0.9
0.8 ± 0.1
0.8
1.2-1.9
1.7 ± 0.0
1.6
1.9-2.4
2.2 ± 0.2
2.1
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Appendix
207
Table 7.23. Measurements for Amphibalanus zhujiangensis (N = 11).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
Mean ± SD (mm) Median (mm)
5.8-21.6
12.6 ± 7.9
13.7
3.0-10.0
6.5 ± 3.1
6.1
2.8-16.5
8.7 ± 4.1
6.9
2.6-7.6
4.9 ± 1.8
4.8
4.8-19.2
10.5 ± 3.7
8.8
1.2-2.4
1.9 ± 0.2
2.2
0.9-2.1
1.5 ± 0.1
2.0
2.0-3.4
2.5 ± 0.7
2.6
1.0-1.7
1.2 ± 0.3
1.4
0.5-1.1
0.8 ± 0.1
0.9
1.0-1.7
1.3 ± 0.2
1.2
0.5-0.9
0.7 ± 0.1
0.7
0.4-1.1
0.6 ± 0.2
0.8
0.7-1.8
1.3 ± 0.4
1.5
1.6-3.1
2.1 ± 0.1
1.8
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Table 7.24. Measurements for Amphibalanus sp. (N = 4).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
Mean ± SD (mm) Median (mm)
7.4-12.2
10.8 ± 2.3
11.9
3.6-8.3
6.0 ± 1.9
6.9
5.5-9.4
7.1 ± 1.6
6.8
2.6-5.9
4.3 ± 1.4
4.3
6.3-11.8
9.4 ± 2.3
9.8
1.4-2.1
1.9 ± 0.3
2.0
1.2-1.8
1.5 ± 0.3
1.5
1.9-3.2
2.6 ± 0.5
2.7
1.0-1.3
1.2 ± 0.1
1.2
0.7-0.9
0.8 ± 0.1
0.9
1.3-1.6
1.4 ± 0.1
1.4
0.6-0.7
0.6 ± 0.1
0.6
0.5-0.7
0.6 ± 0.1
0.6
1.1-1.5
1.3 ± 0.1
1.3
2.0-2.6
2.3 ± 0.3
2.2
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
208
Chapter 7
Table 7.25. Measurements for Megabalanus tintinnabulum (N = 4).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
Mean ± SD (mm) Median (mm)
26.0-49.2
37.3 ± 1.1
1.1
13.7-16.3
15.2 ± 0.1
0.1
20.1-49.4
39.3 ± 16.5
16.5
10.4-15.9
13.1 ± 3.7
3.7
29.0-43.1
37.4 ± 9.9
10.0
1.6-3.1
2.5 ± 0.1
0.1
0.6-1.9
1.1 ± 0.8
0.8
2.3-3.7
2.9 ± 0.9
0.9
0.8-1.3
1.0 ± 0.3
0.3
0.3-0.8
0.4 ± 0.3
0.3
0.9-1.4
1.2 ± 0.4
0.4
0.4-0.5
0.4 ± 0.1
0.1
0.3-0.5
0.4 ± 0.1
0.1
0.8-1.4
1.0 ± 0.3
0.3
2.6-3.1
2.9 ± 0.0
0.0
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Table 7.26. Measurements for Megabalanus zebra (N = 8).
Parameter
LB
LO
H
WO
WB
LB/LO
LB/H
LB/WO
LB/WB
LO/H
LO/WO
LO/WB
WO/H
WB/H
WB/WO
Range (mm)
Mean ± SD (mm) Median (mm)
5.4-19.0
14.3 ± 5.0
8.9
3.1-7.6
6.2 ± 2.8
5.1
4.4-13.1
9.2 ± 5.4
8.2
1.7-6.2
4.4 ± 2.4
3.4
5.0-20.0
13.6 ± 5.6
8.9
1.7-3.0
2.3 ± 0.0
1.7
1.0-2.3
1.6 ± 0.1
1.1
2.5-3.9
3.2 ± 0.5
2.8
0.9-1.2
1.1 ± 0.1
1.0
0.6-1.1
0.7 ± 0.1
0.7
1.2-1.8
1.5 ± 0.3
1.6
0.4-0.6
0.5 ± 0.1
0.6
0.4-0.7
0.5 ± 0.0
0.4
1.1-2.3
1.5 ± 0.0
1.1
2.5-3.8
3.1 ± 0.3
2.7
Key to parameters: LB = basal length; LO = orifice length; H = carinal height; WO = orifice width;
WB = basal width.
Appendix
Table 7.27. Kimura 2-parameter (K2P) distances of COI sequences between species
!Title: Microeuraphia sp1;
!Format DataType=Distance DataFormat=LowerLeft NTaxa=29;
!Description
Analysis ====================
Analysis
Scope
Estimate Variance
= ====================
= Pairs of taxa
= ====================
Variance Estimation Method = Bootstrap method
No. of Bootstrap Replications = 1000
Substitution Model
= ====================
Substitutions Type
= Nucleotide
Model/Method
= Kimura 2-parameter model
Substitutions to Include
= d: Transitions + Transversions
Rates and Patterns
Rates among Sites
Pattern among Lineages
Data Subset to Use
= ====================
= Uniform Rates
= Same (Homogeneous)
= ====================
Gaps/Missing Data Treatment = Pairwise deletion
Select Codon Positions
= 1st,2nd,3rd,Non-Coding
No. of Sites:641
d:Estimate
S.E:Standard error
[ 1] #Microeuraphia_sp1_Bcl3917PM1359_{Microeuraphia_sp1}
[ 2] #Microeuraphia_sp1_Bcl3917PM1358_{Microeuraphia_sp1}
[ 3] #Microeuraphia_sp2_JX083873.1_{Microeuraphia_sp2}
[ 4] #Microeuraphia_sp2_Bcl4917PM1510_{Microeuraphia_sp2}
[ 5] #Microeuraphia_sp2_Bcl5517PM1641_{Microeuraphia_sp2}
[ 6] #Microeuraphia_sp2_X4PM1494_{Microeuraphia_sp2}
[ 7] #Microeuraphia_sp2_Bcl6217PM1725_{Microeuraphia_sp2}
209
210
Chapter 7
[ 8] #Microeuraphia_sp2_Bcl5317PM1625_{Microeuraphia_sp2}
[ 9] #Microeuraphia_sp2_X5PM1581_{Microeuraphia_sp2}
[10] #Chthamalus_moro_Bcl2116PM787_{Chthamalus_moro}
[11] #Chthamalus_moro_Bcl4317PM1388_{Chthamalus_moro}
[12] #Chthamalus_moro_Bcl4116PM902_{Chthamalus_moro}
[13] #Chthamalus_moro_Bcl0417PM14_{Chthamalus_moro}
[14] #Chthamalus_moro_Bcl1417PM289_{Chthamalus_moro}
[15] #Chthamalus_moro_Bcl3316PM872_{Chthamalus_moro}
[16] #Chthamalus_moro_Bcl8816PM1217_{Chthamalus_moro}
[17] #Chthamalus_moro_Bcl1217PM243_{Chthamalus_moro}
[18] #Chthamalus_moro_KJ010437.1_{Chthamalus_moro}
[19] #Chthamalus_moro_KJ010460.1_{Chthamalus_moro}
[20] #Chthamalus_moro_Bcl2717PM613_{Chthamalus_moro}
[21] #Chthamalus_moro_Bcl4317PM1389_{Chthamalus_moro}
[22] #Chthamalus_moro_Bcl5916PM1052_{Chthamalus_moro}
[23] #Chthamalus_moro_Bcl8816PM1218_{Chthamalus_moro}
[24] #Chthamalus_malayensis_EU304446.1_{Chthamalus_malayensis}
[25] #Chthamalus_malayensis_EU304427.1_{Chthamalus_malayensis}
[26] #Pseudoctomeris_sulcata_KC138504.1_{Pseudoctomeris_sulcata}
[27] #Pseudoctomeris_sulcata_KC138503.1_{Pseudoctomeris_sulcata}
[28] #Hexechamaesipho_pilbryi_KC896285.1_{Hexechamaesipho_pilsbry}
[29] #Hexechamaesipho_pilbryi_KC896196.1_{Hexechamaesipho_pilsbry}
[
18
1
19
2
3
4
5
6
20 21 22 23
7
24
[ 1]
[ 2] 0.01741
[ 3] 0.11657 0.11824
[ 4] 0.10524 0.11066 0.10892
[ 5] 0.10143 0.10682 0.10321 0.00628
8
25
9 10 11 12 13
26 27 28 29
14
15
16
17
Appendix
211
[ 6] 0.09957 0.10494 0.10135 0.00786 0.00156
[ 7] 0.10847 0.11391 0.11219 0.01262 0.00942 0.00942
[ 8] 0.11078 0.11625 0.11070 0.01262 0.00944 0.00944 0.00628
[ 9] 0.10506 0.11047 0.10873 0.01103 0.00785 0.00785 0.00784 0.00470
[10] 0.18576 0.18549 0.20097 0.16661 0.16669 0.16669 0.17069 0.17088 0.17088
[11] 0.17655 0.17837 0.19770 0.16001 0.16008 0.16008 0.16590 0.16606 0.16414 0.02269
[12] 0.18663 0.18845 0.19734 0.16398 0.16377 0.16377 0.16962 0.17010 0.16787 0.02436
0.01266
[13] 0.18037 0.18635 0.19521 0.16199 0.16178 0.16178 0.16761 0.16808 0.16586 0.02436
0.01106 0.00470
[14] 0.18037 0.18219 0.19734 0.16001 0.15980 0.15980 0.16561 0.16606 0.16386 0.02269
0.01266 0.01264 0.01104
[15] 0.18453 0.18635 0.19734 0.16799 0.16778 0.16778 0.17366 0.17416 0.17192 0.02269
0.00946 0.00945 0.00786 0.00945
[16] 0.18245 0.18427 0.19521 0.16199 0.16178 0.16178 0.16962 0.17010 0.16787 0.01939
0.00946 0.00945 0.00786 0.00628 0.00628
[17] 0.19313 0.19282 0.19322 0.16808 0.16787 0.16787 0.17579 0.17427 0.17203 0.01772
0.03056 0.03383 0.03217 0.02886 0.03051 0.02721
[18] 0.18419 0.18152 0.19693 0.16432 0.16648 0.16648 0.17357 0.17171 0.16902 0.00774
0.02317 0.02908 0.02709 0.02510 0.02510 0.02116 0.02112
[19] 0.17917 0.17653 0.19437 0.16192 0.16166 0.16166 0.16871 0.16927 0.16417 0.00774
0.02317 0.02709 0.02510 0.02313 0.02313 0.01920 0.01916 0.00951
[20] 0.18663 0.18635 0.19948 0.16598 0.16577 0.16577 0.17164 0.17212 0.16989 0.00638
0.01911 0.02233 0.02070 0.02233 0.01908 0.01908 0.01744 0.00951 0.00759
[21] 0.18663 0.18635 0.19734 0.16598 0.16577 0.16577 0.17164 0.17212 0.16989 0.00478
0.02074 0.02397 0.02233 0.02070 0.02070 0.01746 0.01583 0.00759 0.00568 0.00470
[22] 0.18453 0.18427 0.19734 0.16799 0.16778 0.16778 0.17366 0.17416 0.17192 0.01284
0.02565 0.02890 0.02725 0.02561 0.02561 0.02233 0.01103 0.01530 0.01336 0.01264 0.01104
[23] 0.18873 0.19056 0.20163 0.16799 0.16778 0.16778 0.17366 0.17416 0.17192 0.01122
0.02074 0.02397 0.02233 0.02070 0.02070 0.01746 0.01262 0.01530 0.01336 0.01104 0.00945
0.00786
[24] 0.16067 0.15864 0.14139 0.14065 0.14068 0.13873 0.14844 0.14653 0.14653 0.14783
0.15481 0.15481 0.15481 0.15888 0.16092 0.15481 0.15489 0.14955 0.14718 0.15078 0.14878
0.15279 0.15481
212
Chapter 7
[25] 0.19383 0.19145 0.17812 0.18701 0.18498 0.18710 0.19108 0.19126 0.18701 0.17194
0.18325 0.17903 0.17903 0.17903 0.18537 0.17903 0.18953 0.17079 0.17079 0.17693 0.17693
0.17693 0.18325 0.12208
[26] 0.22366 0.22552 0.22039 0.21550 0.20647 0.20429 0.21455 0.21754 0.21511 0.21094
0.22280 0.22018 0.21798 0.22018 0.22018 0.22239 0.22478 0.21882 0.22416 0.21360 0.21798
0.21360 0.22239 0.22431 0.20447
[27] 0.22798 0.22984 0.22470 0.21310 0.20849 0.20630 0.21659 0.21958 0.21714 0.20451
0.21633 0.21375 0.21157 0.21375 0.21375 0.21593 0.21829 0.20849 0.21372 0.20724 0.21157
0.20724 0.21593 0.22220 0.20434 0.01266
[28] 0.21973 0.22442 0.20993 0.21598 0.21341 0.21109 0.22207 0.22285 0.22025 0.21070
0.21634 0.21827 0.21592 0.21358 0.22299 0.21592 0.22320 0.21187 0.20923 0.21827 0.21827
0.22062 0.22299 0.19075 0.20642 0.20163 0.19951
[29] 0.21341 0.21076 0.21460 0.22582 0.22320 0.22082 0.23194 0.23283 0.23017 0.20402
0.20738 0.21395 0.21162 0.20929 0.20929 0.21162 0.21887 0.20944 0.20680 0.20929 0.20929
0.21162 0.21395 0.18828 0.20182 0.20911 0.20698 0.05390
Estimates of Evolutionary Divergence between Sequences
The number of base substitutions per site from between sequences are shown. Standard error
estimate(s) are shown above the diagonal and were obtained by a bootstrap procedure (1000
replicates). Analyses were conducted using the Kimura 2-parameter model [1]. This analysis
involved 30 nucleotide sequences. Codon positions included were 1st+2nd+3rd+Noncoding.
All ambiguous positions were removed for each sequence pair (pairwise deletion option).
There was a total of 641 positions in the final dataset. Evolutionary analyses were conducted
in MEGA X [2] The presence of n/c in the results denotes cases in which it was not possible to
estimate evolutionary distances.
Table 7.28. Kimura 2-parameter (K2P) distances of COI sequences between species
Title: Amphibalanus sp. with Balanidae COI
Description
Analysis ====================
Analysis
= ====================
Scope
= Pairs of taxa
Estimate Variance
= ====================
Variance Estimation Method
No. of Bootstrap Replications
= Bootstrap method
= 1000
Appendix
Substitution Model
= ====================
Substitutions Type
= Nucleotide
Model/Method
= Kimura 2-parameter model
Substitutions to Include
= d: Transitions + Transversions
Rates and Patterns
Rates among Sites
Pattern among Lineages
Data Subset to Use
Gaps/Missing Data Treatment
Select Codon Positions
= ====================
= Uniform Rates
= Same (Homogeneous)
= ====================
= Pairwise deletion
= 1st,2nd,3rd,Non-Coding
No. of Sites:641
d:Estimate
S.E:Standard error
[ 1] #Amphibalanus_sp_Bcl4117PM1378_{Amphibalanus_sp}
[ 2] #Amphibalanus_sp_Bcl4117PM1379_{Amphibalanus_sp}
[ 3] #Amphibalanus_sp_Bcl4216PM937_{Amphibalanus_sp}
[ 4] #Amphibalanus_sp_Bcl5217PM1550_{Amphibalanus_sp}
[ 5] #Amphibalanus_sp_Bcl1117PM125_{Amphibalanus_sp}
[ 6] #Amphibalanus_amphitrite_Bcl6717PM1780_{Amphibalanus_amphitrite}
[ 7] #Amphibalanus_amphitrite_KM211494.1_{Amphibalanus_amphitrite}
[ 8] #Amphibalanus_amphitrite_JQ035517.1_{Amphibalanus_amphitrite}
[ 9] #Amphibalanus_amphitrite_Bcl6317PM1730_{Amphibalanus_amphitrite}
[10] #Amphibalanus_variegatus_X3PM922_{Amphibalanus_variegatus}
[11] #Amphibalanus_variegatus_Bcl7717PM1874_{Amphibalanus_variegatus}
[12] #Amphibalanus_variegatus_Bcl4416PM953_{Amphibalanus_variegatus}
[13] #Amphibalanus_variegatus_X2PM732_{Amphibalanus_variegatus}
[14] #Amphibalanus_variegatus_Bcl5316PM1004_{Amphibalanus_variegatus}
[15] #Amphibalanus_variegatus_KC138446.1_{Amphibalanus_variegatus}
[16] #Amphibalanus_variegatus_JQ035521.1_{Amphibalanus_variegatus}
[17] #Amphibalanus_zhujiangensis_Bcl4517PM1405_{Amphibalanus_zhujiangensis}
[18] #Amphibalanus_zhujiangensis_Bcl2316PM803_{Amphibalanus_zhujiangensis}
[19] #Amphibalanus_zhujiangensis_Bcl4517PM1407_{Amphibalanus_zhujiangensis}
213
214
Chapter 7
[20] #Amphibalanus_zhujiangensis_KC138448.1_{Amphibalanus_zhujiangensis}
[21] #Amphibalanus_zhujiangensis_Bcl4517PM1406_{Amphibalanus_zhujiangensis}
[22] #Amphibalanus_zhujiangensis_Bcl1716PM775_{Amphibalanus_zhujiangensis}
[23] #Amphibalanus_zhujiangensis_Bcl3317PM1307_{Amphibalanus_zhujiangensis}
[24] #Amphibalanus_zhujiangensis_Bcl9016PM1222_{Amphibalanus_zhujiangensis}
[25] #Amphibalanus_zhujiangensis_Bcl4517PM1404_{Amphibalanus_zhujiangensis}
[26] #Megabalanus_tintinnabulum_KC138488.1_{Megabalanus_tintinnabulum}
[27] #Megabalanus_tintinnabulum_KC138487.1_{Megabalanus_tintinnabulum}
[28] #Megabalanus_zebra_KX538962.1_{Megabalnus_zebra}
[29] #Megabalanus_zebra_KC138491.1_{Megabalnus_zebra}
[30] #Balanus_trigonus_KU204234.1_{Balanus_trigonus}
[31] #Balanus_trigonus_KU204228.1_{Balanus_trigonus}
[
11
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2
13
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3
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[ 1]
[0.0027203239 ][0.0027203239 ][0.0027203239 ][0.0021470889 ][0.0163870832
][0.0169572036 ][0.0164800858 ][0.0164581631 ][0.0150734784 ][0.0148853128 ]
[0.0149658258 ][0.0149862603 ][0.0149658258 ][0.0156201472 ][0.0150525082 ]
[0.0193402819 ][0.0194672878 ][0.0194878611 ][0.0190547212 ][0.0193368273 ]
[0.0192186041 ][0.0194476690 ][0.0192186041 ][0.0192186041 ][0.0193728797 ]
[0.0197399521 ][0.0177318654 ][0.0172050236 ][0.0177973206 ][0.0179709810 ]
[ 2] 0.0046966974
[0.0000000000 ][0.0000000000 ][0.0016551544 ][0.0164387962
][0.0169862196 ][0.0164481263 ][0.0164158272 ][0.0151328766 ][0.0149621674 ]
[0.0150276614 ][0.0150461009 ][0.0150276614 ][0.0156716558 ][0.0150885071 ]
[0.0193328954 ][0.0194806599 ][0.0194821695 ][0.0190560821 ][0.0193209599 ]
[0.0192014184 ][0.0194247013 ][0.0192014184 ][0.0192014184 ][0.0194291114 ]
[0.0197770490 ][0.0177400219 ][0.0173374427 ][0.0178503279 ][0.0180220074 ]
[ 3] 0.0046966974 0.0000000000
[0.0000000000 ][0.0016551544 ][0.0164387962
][0.0169862196 ][0.0164481263 ][0.0164158272 ][0.0151328766 ][0.0149621674 ]
[0.0150276614 ][0.0150461009 ][0.0150276614 ][0.0156716558 ][0.0150885071 ]
[0.0193328954 ][0.0194806599 ][0.0194821695 ][0.0190560821 ][0.0193209599 ]
[0.0192014184 ][0.0194247013 ][0.0192014184 ][0.0192014184 ][0.0194291114 ]
[0.0197770490 ][0.0177400219 ][0.0173374427 ][0.0178503279 ][0.0180220074 ]
Appendix
215
[ 4] 0.0046966974 0.0000000000 0.0000000000
[0.0016551544 ][0.0164387962
][0.0169862196 ][0.0164481263 ][0.0164158272 ][0.0151328766 ][0.0149621674 ]
[0.0150276614 ][0.0150461009 ][0.0150276614 ][0.0156716558 ][0.0150885071 ]
[0.0193328954 ][0.0194806599 ][0.0194821695 ][0.0190560821 ][0.0193209599 ]
[0.0192014184 ][0.0194247013 ][0.0192014184 ][0.0192014184 ][0.0194291114 ]
[0.0197770490 ][0.0177400219 ][0.0173374427 ][0.0178503279 ][0.0180220074 ]
[ 5] 0.0031268374 0.0015625013 0.0015625013 0.0015625013
[0.0164637380
][0.0169629271 ][0.0164430821 ][0.0164164018 ][0.0150978453 ][0.0149141680
[0.0149908357 ][0.0150103882 ][0.0149908357 ][0.0156408747 ][0.0150796726
[0.0193032739 ][0.0194372170 ][0.0194505723 ][0.0190154763 ][0.0192937268
[0.0191685932 ][0.0193957002 ][0.0191685932 ][0.0191685932 ][0.0193721487
[0.0197394879 ][0.0176753478 ][0.0172907304 ][0.0177869289 ][0.0179527814 ]
]
]
]
]
[ 6] 0.1481065093 0.1499921937 0.1499921937 0.1499921937 0.1480542068
[0.0094555782 ][0.0086465512 ][0.0078733224 ][0.0166872899 ][0.0164196824 ]
[0.0163928960 ][0.0165801745 ][0.0165508226 ][0.0164086139 ][0.0159750893 ]
[0.0195555574 ][0.0197214971 ][0.0197999103 ][0.0195089180 ][0.0196034112 ]
[0.0198198088 ][0.0197999103 ][0.0196034112 ][0.0196034112 ][0.0197458871 ]
[0.0201601852 ][0.0198404902 ][0.0198949386 ][0.0175754589 ][0.0173187862 ]
[ 7] 0.1540002534 0.1539343061 0.1539343061 0.1539343061 0.1518893134
0.0476872705
[0.0040746599 ][0.0045095804 ][0.0173190035 ][0.0171670193
][0.0170978367 ][0.0172487556 ][0.0172487556 ][0.0181083253 ][0.0176084539 ]
[0.0205799638 ][0.0205903308 ][0.0206962059 ][0.0203055526 ][0.0204793653 ]
[0.0206655608 ][0.0206962059 ][0.0204793653 ][0.0204793653 ][0.0204954790 ]
[0.0211430233 ][0.0196465331 ][0.0197028965 ][0.0187935937 ][0.0186730238 ]
[ 8] 0.1506313451 0.1505695796 0.1505695796 0.1505695796 0.1486202594
0.0421422092 0.0098870053
[0.0027910836 ][0.0164774660 ][0.0163106626
][0.0162759696 ][0.0164360045 ][0.0164127935 ][0.0170803427 ][0.0166382471 ]
[0.0200469158 ][0.0200961733 ][0.0201848617 ][0.0198396763 ][0.0199964494 ]
[0.0202036648 ][0.0201848617 ][0.0199964494 ][0.0199964494 ][0.0200061706 ]
[0.0205834516 ][0.0197164000 ][0.0192197126 ][0.0180767109 ][0.0180859806 ]
[ 9] 0.1501760779 0.1501095597 0.1501095597 0.1501095597 0.1481640320
0.0370236881 0.0115707643 0.0047114497
[0.0164340532 ][0.0161942379
][0.0161660278 ][0.0163294162 ][0.0163047465 ][0.0165756112 ][0.0161373645 ]
[0.0200310676 ][0.0200749267 ][0.0201536609 ][0.0198916473 ][0.0199612211 ]
[0.0201643822 ][0.0201536609 ][0.0199612211 ][0.0199612211 ][0.0194711483 ]
[0.0200600794 ][0.0194321009 ][0.0190463534 ][0.0178133503 ][0.0176562801 ]
216
Chapter 7
[10] 0.1320893711 0.1339470093 0.1339470093 0.1339470093 0.1320386100
0.1454147087 0.1526468513 0.1434116583 0.1415311940
[0.0034725341
][0.0015096842 ][0.0000000000 ][0.0000000000 ][0.0055528015 ][0.0054583057 ]
[0.0194580020 ][0.0196559067 ][0.0195424522 ][0.0190785481 ][0.0193308308 ]
[0.0191754862 ][0.0193691996 ][0.0191754862 ][0.0191754862 ][0.0172386246 ]
[0.0174469355 ][0.0196200705 ][0.0175034845 ][0.0162798411 ][0.0165089449 ]
[11] 0.1294040438 0.1312415920 0.1312415920 0.1312415920 0.1293476504 0.1424806507
0.1500426640 0.1409893246 0.1386418603 0.0078940078
[0.0030738622
][0.0034565516 ][0.0034511954 ][0.0057197866 ][0.0057835924 ][0.0192876854 ]
[0.0195525360 ][0.0194038630 ][0.0190127787 ][0.0191897770 ][0.0190329633 ]
[0.0192205475 ][0.0190329633 ][0.0190329633 ][0.0174788509 ][0.0176988018 ]
[0.0188068238 ][0.0175412856 ][0.0162419618 ][0.0161106137 ]
[12] 0.1311865825 0.1330305084 0.1330305084 0.1330305084 0.1311365668
0.1424806507 0.1500426640 0.1409893246 0.1386418603 0.0015723283 0.0062671794
[0.0015024289 ][0.0015003460 ][0.0053290060 ][0.0052307562 ][0.0190534077 ]
[0.0192637659 ][0.0191486927 ][0.0187893214 ][0.0189473717 ][0.0187980671 ]
[0.0189911976 ][0.0187980671 ][0.0187980671 ][0.0173853952 ][0.0175926718 ]
[0.0191776279 ][0.0175962334 ][0.0163314003 ][0.0162034047 ]
[13] 0.1314111174 0.1332584523 0.1332584523 0.1332584523 0.1313609168
0.1446607380 0.1520918472 0.1431634535 0.1407997149 0.0000000000 0.0078567944
0.0015649465
[0.0000000000 ][0.0055424034 ][0.0054493397 ][0.0193080947
][0.0195059609 ][0.0193907698 ][0.0190336370 ][0.0191927165 ][0.0190371555 ]
[0.0192282884 ][0.0190371555 ][0.0190371555 ][0.0172228685 ][0.0174361602 ]
[0.0194705398 ][0.0174781079 ][0.0161780995 ][0.0164093396 ]
[14] 0.1311865825 0.1330305084 0.1330305084 0.1330305084 0.1311365668
0.1444111551 0.1520918472 0.1429161093 0.1405575714 0.0000000000 0.0078444677
0.0015625013 0.0000000000
[0.0055345819 ][0.0054407380 ][0.0192799757
][0.0194774941 ][0.0193636825 ][0.0190058227 ][0.0191669382 ][0.0190111561 ]
[0.0192022511 ][0.0190111561 ][0.0190111561 ][0.0171889803 ][0.0173995404 ]
[0.0194705398 ][0.0174477435 ][0.0161499297 ][0.0163799183 ]
[15] 0.1393695352 0.1412336481 0.1412336481 0.1412336481 0.1392994097
0.1411012193 0.1582902736 0.1468567159 0.1411012193 0.0175475102 0.0190665065
0.0158761879 0.0175195597 0.0174916982
[0.0030743056 ][0.0190327479
][0.0192450920 ][0.0190979381 ][0.0187311393 ][0.0189354614 ][0.0187991886 ]
[0.0189802020 ][0.0187991886 ][0.0187991886 ][0.0175879378 ][0.0177665301 ]
[0.0185879866 ][0.0181830432 ][0.0165038111 ][0.0163001310 ]
Appendix
217
[16] 0.1317522722 0.1336002405 0.1336002405 0.1336002405 0.1316918412
0.1373112915 0.1521708290 0.1411012193 0.1353955803 0.0175251242 0.0190492388
0.0158565501 0.0174972452 0.0174694547 0.0062912298
[0.0188867678
][0.0191192123 ][0.0189650208 ][0.0185984057 ][0.0187848052 ][0.0186586884 ]
[0.0188550660 ][0.0186586884 ][0.0186586884 ][0.0175932934 ][0.0176645435 ]
[0.0185402687 ][0.0179568123 ][0.0161893364 ][0.0159697234 ]
[17] 0.1818428275 0.1817388880 0.1817388880 0.1817388880 0.1796854970 0.1958726603
0.2026672331 0.2009364512 0.2003390586 0.1832552970 0.1819523093 0.1798904508
0.1822763051 0.1819523093 0.1804250026 0.1764158181
[0.0040851228
][0.0035857935 ][0.0035597855 ][0.0038230480 ][0.0039098076 ][0.0039071329 ]
[0.0035506240 ][0.0035506240 ][0.0195997074 ][0.0195510828 ][0.0194240002 ]
[0.0183544702 ][0.0196728011 ][0.0192437511 ]
[18] 0.1839089462 0.1838007466 0.1838007466 0.1838007466 0.1817388880
0.2000920946 0.2026672331 0.2009364512 0.2003390586 0.1853429827 0.1840227057
0.1819523093 0.1843509969 0.1840227057 0.1824911212 0.1784692091 0.0094491001
[0.0035707469 ][0.0035525551 ][0.0038279762 ][0.0039251395 ][0.0039225783 ]
[0.0035607254 ][0.0035607254 ][0.0192823004 ][0.0192125343 ][0.0194462417 ]
[0.0180351736 ][0.0195758351 ][0.0192164712 ]
[19] 0.1818428275 0.1817388880 0.1817388880 0.1817388880 0.1796854970
0.2000920946 0.2026672331 0.2009364512 0.2003390586 0.1811762919 0.1798904508
0.1778370598 0.1802101864 0.1798904508 0.1783673865 0.1743708255 0.0078617972
0.0078617972
[0.0031606720 ][0.0034766762 ][0.0035136677 ][0.0027265842
][0.0031599672 ][0.0031599672 ][0.0194732433 ][0.0194144965 ][0.0191465966 ]
[0.0183114693 ][0.0196521468 ][0.0192756694 ]
[20] 0.1763182033 0.1762261216 0.1762261216 0.1762261216 0.1741894589
0.1965775720 0.1982128607 0.1966901412 0.1968084919 0.1770440370 0.1764158181
0.1743708255 0.1767293661 0.1764158181 0.1742773842 0.1703057625 0.0078865988
0.0078865988 0.0062992959
[0.0027359803 ][0.0028424893 ][0.0028259279
][0.0022944165 ][0.0022944165 ][0.0191311237 ][0.0190851324 ][0.0185852402 ]
[0.0178969899 ][0.0195837810 ][0.0191799545 ]
[21] 0.1818428275 0.1817388880 0.1817388880 0.1817388880 0.1796854970
0.1979779265 0.2004350866 0.1988087885 0.1982159132 0.1791058956 0.1778370598
0.1757920672 0.1781525704 0.1778370598 0.1763182033 0.1723341628 0.0094491001
0.0094491001 0.0078617972 0.0047170161
[0.0021691738 ][0.0021744601
][0.0014562994 ][0.0014562994 ][0.0193744726 ][0.0193340828 ][0.0188955785 ]
[0.0181880834 ][0.0194529169 ][0.0190603609 ]
218
Chapter 7
[22] 0.1797852114 0.1796854970 0.1796854970 0.1796854970 0.1776405044
0.2000920946 0.2026672331 0.2009364512 0.2003390586 0.1770440370 0.1757920672
0.1737554045 0.1761033872 0.1757920672 0.1742773842 0.1703057625 0.0094491001
0.0094491001 0.0078617972 0.0047170161 0.0031299007
[0.0022126512
][0.0015891449 ][0.0015891449 ][0.0193138669 ][0.0192624396 ][0.0189971992 ]
[0.0181461927 ][0.0193437138 ][0.0189529137 ]
[23] 0.1818428275 0.1817388880 0.1817388880 0.1817388880 0.1796854970 0.2000920946
0.2026672331 0.2009364512 0.2003390586 0.1791058956 0.1778370598 0.1757920672
0.1781525704 0.1778370598 0.1763182033 0.1723341628 0.0094491001 0.0094491001
0.0047022290 0.0047170161 0.0031299007 0.0031299007
[0.0016375586
][0.0016375586 ][0.0194732433 ][0.0194144965 ][0.0192293861 ][0.0183744474 ]
[0.0196206270 ][0.0192451575 ]
[24] 0.1797852114 0.1796854970 0.1796854970 0.1796854970 0.1776405044
0.1979779265 0.2004350866 0.1988087885 0.1982159132 0.1770440370 0.1757920672
0.1737554045 0.1761033872 0.1757920672 0.1742773842 0.1703057625 0.0078617972
0.0078617972 0.0062795174 0.0031397277 0.0015625013 0.0015625013 0.0015625013
[0.0000000000 ][0.0193138669 ][0.0192624396 ][0.0189971992 ][0.0181461927 ]
[0.0193437138 ][0.0189529137 ]
[25] 0.1797852114 0.1796854970 0.1796854970 0.1796854970 0.1776405044
0.1979779265 0.2004350866 0.1988087885 0.1982159132 0.1770440370 0.1757920672
0.1737554045 0.1761033872 0.1757920672 0.1742773842 0.1703057625 0.0078617972
0.0078617972 0.0062795174 0.0031397277 0.0015625013 0.0015625013 0.0015625013
0.0000000000
[0.0193138669 ][0.0192624396 ][0.0189971992 ][0.0181461927 ]
[0.0193437138 ][0.0189529137 ]
[26] 0.1811443398 0.1830983642 0.1830983642 0.1830983642 0.1810106785
0.1970624698 0.1963987291 0.1993348192 0.1930840283 0.1571649812 0.1587847912
0.1586036706 0.1568898057 0.1566155964 0.1625130923 0.1624278429 0.1841793867
0.1799510317 0.1841793867 0.1799510317 0.1841793867 0.1820607395 0.1841793867
0.1820607395 0.1820607395
[0.0032700255 ][0.0187442918 ][0.0112497462 ]
[0.0203508620 ][0.0203120752 ]
[27] 0.1832363931 0.1851948033 0.1851948033 0.1851948033 0.1830983642
0.1991946692 0.2031102393 0.2058004266 0.1994807040 0.1591609919 0.1607888019
0.1605996812 0.1588818402 0.1586036706 0.1645211271 0.1624278429 0.1820607395
0.1778501883 0.1820607395 0.1778501883 0.1820607395 0.1799510317 0.1820607395
0.1799510317 0.1799510317 0.0062992959
[0.0192920869 ][0.0112824294 ]
[0.0201076136 ][0.0200796057 ]
Appendix
219
[28] 0.1514179949 0.1533350821 0.1533350821 0.1533350821 0.1513310714
0.1913834748 0.1939194566 0.1943235990 0.1915982140 0.1788101577 0.1694309108
0.1754321725 0.1775198583 0.1775198583 0.1697317196 0.1698291312 0.1784738060
0.1806014687 0.1784738060 0.1727681533 0.1742454510 0.1763551587 0.1784738060
0.1763551587 0.1763551587 0.1617377892 0.1680669876
[0.0185028930 ]
[0.0202375222 ][0.0200911423 ]
[29] 0.1548007064 0.1566995534 0.1566995534 0.1566995534 0.1547154239 0.1927042520
0.1852943740 0.1844567601 0.1824911212 0.1572495808 0.1568832618 0.1586915880
0.1569740831 0.1566995534 0.1646157670 0.1625130923 0.1673404115 0.1612303558
0.1673404115 0.1612303558 0.1673404115 0.1652954189 0.1673404115 0.1652954189
0.1652954189 0.0731271784 0.0731271784 0.1615694039
[0.0202809524 ]
[0.0203104232 ]
[30] 0.1642434099 0.1661845656 0.1661845656 0.1661845656
0.1639981665 0.1750834737 0.1689184426 0.1663727911 0.1491672134
0.1500807670 0.1483832908 0.1481238174 0.1505648833 0.1486436780
0.1880650075 0.1880650075 0.1866531274 0.1859816711 0.1839069793
0.1839069793 0.1839069793 0.2003876052 0.1982136887 0.2017444704
[0.0035837854 ]
0.1641561653
0.1482185610
0.1901570607
0.1859816711
0.1961974277
[31] 0.1662759330 0.1682212283 0.1682212283 0.1682212283
0.1619860914 0.1750834737 0.1709760587 0.1643361284 0.1511396026
0.1481238174 0.1503440776 0.1500807670 0.1486040965 0.1466867283
0.1839069793 0.1839069793 0.1824950993 0.1818408607 0.1797832446
0.1797832446 0.1797832446 0.1982136887 0.1960491831 0.1995985443
0.0079619517
0.1661845656
0.1462616114
0.1859816711
0.1818408607
0.1983666285
Estimates of Evolutionary Divergence between Sequences
The number of base substitutions per site from between sequences are shown. Standard
error estimate(s) are shown above the diagonal. Analyses were conducted using the Kimura
2-parameter model [1]. This analysis involved 31 nucleotide sequences. Codon positions
included were 1st+2nd+3rd+Noncoding. All ambiguous positions were removed for each
sequence pair (pairwise deletion option). There was a total of 641 positions in the final dataset.
Evolutionary analyses were conducted in MEGA X [2]
1.
2.
Kimura M. (1980). A simple method for estimating evolutionary rate of base substitutions
through comparative studies of nucleotide sequences. Journal of Molecular Evolution
16:111-120.
Kumar S., Stecher G., Li M., Knyaz C., and Tamura K. (2018). MEGA X: Molecular
Evolutionary Genetics Analysis across computing platforms. Molecular Biology and
Evolution 35:1547-1549.
220
Chapter 7
Figure 7.1. The occurrence of Heteralepas japonica in the Moluccas (red dots).
Figure 7.2. The occurrence of Amphibalanus reticulatus, Dosima fascicularis, Euraphia hembeli,
Hexechamaesipho pilsbryi, Megabalanus zebra, Nesochthamalus intertextus, Newmanella
spinosus, Pseudoctomeris sulcata, Tetraclitella divisa and Tetraclitella karandei in the Moluccas
(the red dot indicates that the species occurs on the coastline of the island).
Appendix
221
Figure 7.3. The occurrence of Chthamalus moro and Lepas anserifera in the Moluccas (the red
dots indicate that the species occurs on the coastline of the islands).
Figure 7.4. The occurrence of Amphibalanus amphitrite, Amphibalanus variegatus, Capitulum
mitella, Megabalanus tintinnabulum, Tesseropora rosea, Tetraclita kuroshioensis and Tetraclita
squamosa in the Moluccas (the red dots indicate that the species occurs on the coastline of
the islands).
222
Chapter 7
Figure 7.5. The occurrence of Microeuraphia sp. in the Moluccas (the red dot indicates that
the species occurs on the coastline of the island).
Figure 7.6. The occurrence of Yamaguchiella coerulescens in the Moluccas (the red dots
indicate that the species occurs on the coastline of the islands).
Appendix
223
Figure 7.7. The occurrence of Neonrosella vitiata in the Moluccas (the red dots indicate that
the species occurs on the coastline of the islands).
Figure 7.8. The occurrence of Amphibalanus zhujiangensis in the Moluccas (the red dots
indicate that the species occurs on the coastline of the islands).
224
Chapter 7
Figure 7.9. The occurrence of Amphibalanus sp. in the Moluccas (the red dots indicate that the
species occurs on the coastline of the islands).
Appendix
225
Figure 7.10. Bayesian phylogeny of concatenated COI and 18S gene sequences. Values next to
nodes are support values.
226
Chapter 7
Figure 7.11. ML phylogeny of concatenated COI and 18S gene sequences. Values next to nodes
are support values.
Appendix
227
Figure 7.12. ML phylogeny of 18S gene sequences. Tips coloured in red are the new samples
sequenced for this paper. Values next to nodes are support values.
228
Chapter 7
Figure 7.13. ML phylogeny of COI gene sequences. Tips coloured in red are the new samples
sequenced for this paper. Values next to nodes are support values.
Appendix
229
7. 2 Supplementary material for New insights gained from museum
collections: Deep-sea barnacles (Crustacea, Cirripedia,
Thoracica) in the Muséum National d’Histoire Naturelle, Paris,
collected during the Karubar expedition in 1991
Table 7.29. Checklist of barnacle species collected during the Karubar expedition (1991) and
deposited in MNHN, Paris.
ORDER
Suborder
LEPADIFORMES
Heteralepadomorpha
Lepadomorpha
SCALPELLIFORMES
Family
Subfamily
Species
Depth
in
meter
Heteralepadidae
Heteralepas
japonica (Aurivillius,
1892)
271–
273
Paralepas minuta
(Philippi, 1836)
240–
346
Paralepas morula
(Hoek, 1907)
Dianajonesia
amygdalum
(Aurivillius, 1894)
Dianajonesia
excavatum (Hoek,
1907)
Dichelaspis
orthogonia Darwin,
1852
Glyptelasma gracile
(Hoek, 1883)
Megalasma
striatum Hoek, 1883
Diotascalpellum
rubrum (Hoek,
1883)
Regioscalpellum
moluccanum (Hoek,
1883)
836–
869
Poecilasmatidae
Scalpellidae
Scalpellinae
Scalpellum sp.
Scalpellidae
Meroscalpellinae
Scalpellum stearnsi
Pilsbry, 1890
Alcockianum
persona
(Annandale, 1916)
250–
239
Substrate
Attached to dead
stem of gorgonian
and spine of sea
urchin
Attached to spine of
sea urchin
Attached to crab leg
295–
284
230–
314
603–
869
206–
459
Attached to spines
of sea urchins
Attached to spines
of sea urchin
330–
389
Attached to
gorgonian
206–
1266
210–
206
287–
480
Attached to glass
rope sponge
477–
620
Attached to glass
rope sponge
230
Chapter 7
Scalpellidae
Arcoscalpellinae
SESSILIA
Verrucomorpha
Verrucidae
Annandaleum
japonicum (Hoek,
1883)
Annandaleum
laccadivicum
(Annandale, 1906)
Annandaleum
lambda (Annandale,
1910)
Litoscalpellum juddi
(Calman, 1918)
Litoscalpellum
recurvirostrum
(Hoek, 1883)
Litoscalpellum
walleni Newman &
Ross, 1971
Amigdoscalpellum
costellatum
(Withers, 1935)
Amigdoscalpellum
tenue (Hoek, 1883)
Catherinum rossi
(Rao & Newman,
1972)
Planoscalpellum
distinctum (Hoek,
1883)
Teloscalpellum
ecaudatum
(Calman, 1918)
Trianguloscalpellum
balanoides (Hoek,
1883)
Trianguloscalpellum
hirsutum (Hoek,
1883)
Verum australicum
(Hoek, 1883)
Verum carinatum
(Hoek, 1883)
Verum
novaezelandiae
(Hoek, 1883)
Altiverruca navicula
(Hoek, 1913)
1026–
1053
836–
869
Attached to
gastropod shell
1058–
1266
Attached to pumice
477–
699
285–
299
884–
891
688–
694
Attached to glass
rope glass
769–
809
209–
240
210–
206
390–
502
223–
349
Attached to crinoid
884–
891
1084–
1058
205–
212
1017–
1024
884–
891
Attached to
peduncle of
Alcockianum
persona (Annandale,
1916)
Appendix
Balanomorpha
Pachylasmatidae
Hexelasmatinae
Archaeobalanidae
Archaeobalaninae
Newmaniverruca
albatrossiana
(Pilsbry, 1912)
Hexelasma arafurae
Hoek, 1913
Conopea
cymbiformis
(Darwin, 1854)
Conopea navicula
(Darwin, 1854)
Solidobalanus
auricoma (Hoek,
1913)
Solidobalanus
pseudauricoma
(Broch, 1931)
Striatobalanus
amaryllis (Darwin,
1854)
Striatobalanus
tenuis (Hoek, 1883)
Pyrgomatidae
Megatrematinae
Pyrgomina sp.
Balanidae
Amphibalaninae
Amphibalanus
amphitrite (Darwin,
1854)
688–
694
231
Attached to glass
rope sponge
205–
212
111–
850
206–
220
Attached to
gorgonian
Attached to lateral
plate of Chirona
tenuis, spines
206–
of sea urchin,
502
antipatharian, arm
of crinoid, and dead
gorgonian
Attached to
gorgonian, coral,
82–349
crinoid, and spines
of sea urchins
174–
176
Attached to broken
sea urchin shell
174–
389
225–
223
Attached to shell of
gastropod shell
Attached to coral
1244–
1266
Table 7.30. Measurements for Heteralepas japonica (Aurivillius, 1892) (N = 1).
No
Sample
number
1
MNHNIU-2019-4877
a
b
18.50
12.82
Parameter
c
d
15.26
8.46
e
5.97
f
5.55
Key to parameters: a = capitulum height; b = capitulum width; c = peduncle length; d = orifice
height; e = capitulum thickness; f = peduncle width.
Table 7.31. Measurements for Paralepas minuta (Philippi, 1836) (N = 1).
No
Sample
number
1
MNHNIU-2019-4882
a
b
8.34
6.67
Parameter
c
d
3.84
3.31
e
f
4.53
2.60
Key to parameters: a = capitulum height; b = capitulum width; c = peduncle length; d = orifice
height; e = capitulum thickness; f = peduncle width.
Chapter 7
232
Table 7.32. Measurements for Paralepas morula (Hoek, 1907) (N = 1).
No
Sample
number
1
MNHNIU-2019-4878
a
b
5.14
3.69
Parameter
c
d
5.08
2.02
e
f
1.81
1.31
Key to parameters: a = capitulum height; b = capitulum width; c = peduncle length; d = orifice
height; e = capitulum thickness; f = peduncle width.
Table 7.33. Measurements for Dianajonesia amygdalum (Aurivillius, 1894) (N = 1).
No
Sample
number
1
MNHNIU-2019-4861
TH
CH
DBC
10.40
7.03
0.94
Parameter
CS
LS
4.49
5.85
WS
LT
WT
4.11
3.13
1.00
Key to parameters: TH = total height; CH = capitulum height; DBC = diameter of the base of
capitulum; LS = scutum length; WS = scutum width; LT = tergum length; WT = tergum width.
Table 7.34. Measurements for Dianajonesia excavatum (Hoek, 1907) (N = 1).
No
Sample
number
1
MNHNIU-2019-4859
TH
CH
DBC
12.69
7.12
1.06
Parameter
CS
LS
3.95
6.09
WS
LT
WT
3.08
3.47
1.30
Key to parameters: TH = total height; CH = capitulum height; DBC = diameter of the base of
capitulum; LS = scutum length; WS = scutum width; LT = tergum length; WT = tergum width.
Table 7.35. Measurements for Dichelaspis orthogonia Darwin, 1852 (N = 1).
No
Sample
number
1
MNHNIU-2019-4864
TH
CH
DBC
10.47
8.69
1.89
Parameter
CS
LS
4.75
7.16
WS
LT
WT
3.5
6.53
0.80
Key to parameters: TH = total height; CH = capitulum height; DBC = diameter of the base of
capitulum; LS = scutum length; WS = scutum width; LT = tergum length; WT = tergum width.
Table 7.36. Measurements for Glyptelasma gracile (Hoek, 1883) (N = 1).
No
Sample
number
1
MNHNIU-2019-4876
TH
CH
DBC
13.99
10.44
1.45
Parameter
CS
LS
5.16
9.01
WS
LT
WT
4.79
5.07
1.87
Key to parameters: TH = total height; CH = capitulum height; DBC = diameter of the base of
capitulum; LS = scutum length; WS = scutum width; LT = tergum length; WT = tergum width.
Appendix
233
Table 7.37. Measurements for Megalasma striatum Hoek, 1883 (N = 1).
No
Sample
number
1
MNHNIU-209-4870
TH
CH
DBC
9.36
9.36
2.14
Parameter
CS
LS
4.82
7.28
WS
LT
WT
3.81
4.29
1.58
Key to parameters: TH = total height; CH = capitulum height; DBC = diameter of the base of
capitulum; LS = scutum length; WS = scutum width; LT = tergum length; WT = tergum width.
Table 7.38. Measurements for Diotascalpellum rubrum (Hoek, 1883) (N = 2).
No
Sample number
1
MNHNIU-2019-4925
MNHNIU-2019-4926
2
a
b
20.57
10.55
12.51
7.39
Parameter
c
d
e
6.51
5.10
6.62
4.09
7.76
4.36
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.39. Measurements for Regioscalpellum moluccanum (Hoek, 1883) (N = 5).
No
Sample number
1
MNHNIU-2019-4909-1
MNHNIU-2019-4909-2
MNHNIU-2019-4909-3
MNHNIU-2019-4910
MNHNIU-2019-4912
2
3
4
5
a
b
12.92
10.20
8.75
Parameter
c
d
e
4.91
9.11
6.04
5.52
3.11
5.89
3.45
7.88
4.81
2.91
8.43
3.30
8.27
5.40
2.15
3.38
2.09
14.89
10.65
5.24
9.13
5.39
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.40. Measurements for Scalpellum sp. (N = 1).
No
Sample number
1
MNHNIU-2019-4929
a
b
15.90
10.29
Parameter
c
4.58
d
e
6.97
5.67
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Chapter 7
234
Table 7.41. Measurements for Scalpellum stearnsi Pilsbry, 1890 (N = 5).
No
Sample number
1
MNHNIU-2019-4888
MNHNIU-2019-4889
MNHNIU-2019-4892
MNHNIU-2019-4893
MNHNIU-2019-4894
2
3
4
5
a
b
31.69
28.20
44.75
Parameter
c
d
e
11.19
28.29
11.39
35.46
11.93
37.19
14.94
40.43
31.57
17.54
53.18
15.47
39.34
31.15
14.21
33.23
14.42
52.00
40.11
18.56
86.59
17.94
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.42. Measurements for Alcockianum persona (Annandale, 1916) (N = 5).
No
Sample number
1
MNHNIU-2019-4903
MNHNIU-2019-4904-1
MNHNIU-2019-4904-2
MNHNIU-2019-4906-1
MNHNIU-2019-4906-2
2
3
4
5
a
b
21.06
17.72
35.06
Parameter
c
d
e
11.35
14.41
9.54
28.06
18.20
28.67
10.79
27.01
20.04
11.22
23.61
9.72
25.44
19.76
10.19
21.49
8.06
17.71
12.91
4.96
13.42
6.66
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.43. Measurements for Annandaleum japonicum (Hoek, 1883) (N = 4).
No
Sample number
1
MNHNIU-2019-4932-1
MNHNIU-2019-4932-2
MNHNIU-2019-4932-3
MNHNIU-2019-4932-4
2
3
4
a
b
9.56
4.67
11.66
Parameter
c
d
e
1.85
3.71
1.83
5.82
2.53
3.84
2.01
12.04
6.00
2.82
3.37
3.27
12.73
7.13
3.21
4.77
2.75
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Appendix
235
Table 7.44. Measurements for Annandaleum laccadivicum (Annandale, 1906) (N = 1).
No
Sample number
1
MNHNIU-2019-4931
a
b
18.88
11.41
Parameter
c
6.30
d
e
11.45
4.89
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.45. Measurements for Annandaleum lambda (Annandale, 1910) (N = 5).
No
Sample number
1
MNHNIU-2019-4913
MNHNIU-2019-4914
MNHNIU-2019-4915-1
MNHNIU-2019-4915-2
MNHNIU-2019-4915-3
2
3
4
5
a
b
21.76
13.04
16.61
Parameter
c
d
e
5.59
7.74
5.47
8.73
3.17
6.08
3.73
22.94
13.81
6.31
15.27
6.26
24.27
14.49
6.70
11.06
5.81
20.00
12.27
4.80
5.54
4.63
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.46. Measurements for Litoscalpellum juddi (Calman, 1918) (N = 5).
No
Sample number
1
MNHNIU-2019-4896-1
MNHNIU-2019-4896-2
MNHNIU-2019-4896-3
MNHNIU-2019-4896-4
MNHNIU-2019-4898
2
3
4
5
a
b
16.94
11.39
21.21
Parameter
c
d
e
5.20
16.87
5.63
13.78
7.08
17.06
6.33
39.25
28.28
19.89
35.99
16.87
24.17
13.69
8.09
15.02
7.17
30.32
21.09
7.25
52.02
11.42
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Chapter 7
236
Table 7.47. Measurements for Litoscalpellum recurvirostrum (Hoek, 1883) (N = 5).
No
Sample number
1
MNHNIU-2019-4934
MNHNIU-2019-4935-1
MNHNIU-2019-4935-2
MNHNIU-2019-4937-1
MNHNIU-2019-4937-2
2
3
4
5
a
b
16.15
10.39
16.78
Parameter
c
d
e
5.07
8.37
4.96
10.49
5.25
10.02
5.10
16.39
9.24
4.45
7.25
4.13
26.05
16.29
8.18
20.90
8.95
24.26
16.59
7.59
19.14
8.70
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.48. Measurements for Litoscalpellum walleni Newman & Ross, 1971 (N = 1).
No
Sample number
1
MNHNIU-2019-4927
a
b
14.56
8.09
Parameter
c
2.50
d
e
7.52
3.89
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.49. Measurements for Amigdoscalpellum costellatum (Withers, 1935) (N = 2).
No
Sample number
1
MNHNIU-2019-4922-1
MNHNIU-2019-4922-2
2
a
b
12.44
7.52
13.88
6.97
Parameter
c
d
e
3.47
3.15
2.94
2.77
2.99
3.24
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.50. Measurements for Amigdoscalpellum tenue (Hoek, 1883) (N = 1).
No
Sample number
1
MNHNIU-2019-4921
a
b
7.26
3.74
Parameter
c
1.40
d
e
3.00
1.99
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Appendix
237
Table 7.51. Measurements for Catherinum rossi (Rao & Newman, 1972) (N = 1).
No
Sample number
1
MNHNIU-2019-4933
a
b
9.67
5.52
Parameter
c
2.58
d
e
2.79
2.29
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.52. Measurements for Planoscalpellum distinctum (Hoek, 1883) (N = 2).
No
Sample number
1
MNHNIU-2019-4928-1
MNHNIU-2019-4928-2
2
a
b
6.80
3.68
7.90
4.36
Parameter
c
d
e
1.26
1.39
1.80
1.24
2.49
2.04
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.53. Measurements for Teloscalpellum ecaudatum (Calman, 1918) (N = 2).
No
Sample number
1
MNHNIU-2019-4917
MNHNIU-2019-4918
2
a
b
12.07
6.17
18.59
11.47
Parameter
c
d
e
2.68
8.33
3.21
5.41
6.30
5.41
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.54. Measurements for Trianguloscalpellum balanoides (Hoek, 1883) (N = 1).
No
Sample number
1
MNHNIU-2019-4923
a
b
8.20
4.40
Parameter
c
1.75
d
e
5.06
2.10
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.55. Measurements for Trianguloscalpellum hirsutum (Hoek, 1883) (N = 1).
No
Sample number
1
MNHNIU-2019-4908
a
b
12.18
6.50
Parameter
c
3.74
d
e
4.10
3.43
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Chapter 7
238
Table 7.56. Measurements for Verum australicum (Hoek, 1883) (N = 1).
No
Sample number
1
MNHNIU-2019-4930
a
b
10.33
6.09
Parameter
c
2.08
d
e
4.62
2.24
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.57. Measurements for Verum carinatum (Hoek, 1883) (N = 1).
No
Sample number
1
MNHNIU-2019-4920
a
b
9.14
4.53
Parameter
c
1.74
d
e
2.54
2.20
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.58. Measurements for Verum novaezelandiae (Hoek, 1883) (N = 1).
No
Sample number
1
MNHNIU-2019-4919
a
b
13.46
6.98
Parameter
c
2.99
d
e
6.02
3.23
Key to parameters: a = capitulum height; b = capitulum width; c = capitulum thickness; d =
peduncle length; e = diameter of peduncle.
Table 7.59. Measurements for Hexelasma arafurae Hoek, 1913 (N = 1).
No
Sample number
1
MNHNIU-2019-4951
LB
LO
14.32
8.00
Parameter
H
12.39
WO
WB
6.49
13.76
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Table 7.60. Measurements for Conopea cymbiformis (Darwin, 1854) (N = 1).
No
Sample number
1
MNHNIU-2019-4824
LB
LO
7.73
4.60
Parameter
H
8.70
WO
WB
3.88
4.94
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Appendix
239
Table 7.61. Measurements for Conopea navicula (Darwin, 1854) (N = 4).
No
Sample number
1
MNHNIU-2019-4822-1
MNHNIU-2019-4822-2
MNHNIU-2019-4822-3
MNHNIU-2019-4822-4
2
3
4
LB
LO
3.75
2.02
3.40
Parameter
H
WO
WB
2.38
1.59
2.62
1.61
2.75
0.94
2.61
2.23
1.09
2.04
1.22
1.79
4.22
1.57
3.09
0.94
2.80
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Table 7.62. Measurements for Solidobalanus auricoma (Hoek, 1913) (N = 5).
No
1
2
3
4
5
Sample number
MNHNIU-2019-4850-1
MNHNIU-2019-4850-2
MNHNIU-2019-4850-3
MNHNIU-2019-4850-4
MNHNIU-2019-4850-5
LB
LO
8.18
5.83
6.75
Parameter
H
WO
WB
5.61
3.14
5.24
4.18
4.39
2.58
4.92
6.11
4.51
5.24
2.59
4.93
6.36
4.74
4.11
2.72
4.51
7.29
4.62
6.30
3.19
5.96
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Table 7.63. Measurements for Solidobalanus pseudauricoma (Broch, 1931) (N = 5).
No
Sample number
1
MNHNIU-2019-4818-1
MNHNIU-2019-4818-2
MNHNIU-2019-4818-3
MNHNIU-2019-4818-4
MNHNIU-2019-4818-5
2
3
4
5
LB
LO
11.25
9.62
11.37
Parameter
H
WO
WB
11.28
6.33
9.13
7.52
13.22
5.22
7.80
12.08
9.29
11.42
5.88
9.56
6.50
5.34
3.86
3.66
5.98
6.23
4.63
3.48
3.24
5.66
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Chapter 7
240
Table 7.64. Measurements for Striatobalanus amaryllis (Darwin, 1854) (N = 1).
No
Sample number
1
MNHNIU-2019-4814
LB
LO
16.76
9.13
Parameter
H
9.89
WO
WB
7.84
15.14
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Table 7.65. Measurements for Striatobalanus tenuis (Hoek, 1883) (N = 5).
No
1
2
3
4
5
Sample number
MNHNIU-2019-4820-1
MNHNIU-2019-4820-2
MNHNIU-2019-4820-3
MNHNIU-2019-4820-4
MNHNIU-2019-4833
LB
LO
25.78
14.37
18.92
Parameter
H
WO
WB
18.02
11.80
28.03
13.78
18.31
10.15
20.51
28.30
15.11
18.68
11.85
27.39
24.84
16.29
19.06
12.10
24.32
27.72
15.93
20.24
13.70
25.10
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Table 7.66. Measurements for Pyrgomina sp. (N = 1).
No
Sample number
1
MNHNIU-2019-4817
LB
LO
7.46
2.42
Parameter
H
10.45
WO
WB
1.41
6.15
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Table 7.67. Measurements for Amphibalanus amphitrite (Darwin, 1854) (N = 4).
No
Sample number
1
MNHNIU-2019-4815-1
MNHNIU-2019-4815-2
MNHNIU-2019-4815-3
MNHNIU-2019-4815-4
2
3
4
LB
LO
12.10
7.89
6.25
Parameter
H
WO
WB
5.65
5.22
12.03
4.36
3.82
2.72
6.32
5.27
3.81
3.64
3.01
4.62
6.02
4.15
5.13
3.04
4.92
Key to parameters: LB = basal lenght; LO = orifice lenght; H = carinal heigth; WO = orifice width;
WB = basal width.
Appendix
241
7.3 List of publications, presentations, seminars, symposiums,
trainings, courses, and interviews
International peer-reviewed articles
Pitriana, P., Valente, L., von Rintelen, T., Jones, D.S., Prabowo, R.E. & von Rintelen, K. 2020. An annotated
checklist and integrative biodiversity discovery of barnacles (Crustacea, Cirripedia) from the
Moluccas, East Indonesia. ZooKeys, 945: 17–83. https://doi.org/10.3897/zookeys.945.39044.
Pitriana, P., Jones, D.S., Corbari, L. & von Rintelen, K. 2020. New insights gained from museum collections:
Deep-sea barnacles (Crustacea, Cirripedia, Thoracica) in the Muséum National d’Histoire Naturelle,
Paris, collected during the Karubar expedition in 1991. Zoosystematics and Evolution, 96(2): 649–
698. https://doi.org/10.3897/zse.96.55733.
Pitriana, P., Wessel, A., Aschenbach, T. & von Rintelen, K. 2020. Exploring sponge-inhabiting barnacles
of eastern Indonesia using micro-CT scanning. Journal of Treubia, submitted.
Pitriana, P., von Rintelen, K., Wessel, A. 2020. Non-invasive 3D visualization of the sponge-inhabiting
barnacle Acasta sulcata (Crustacea, Cirripedia, Balanomorpha) from the Moluccas, Indonesia.
[Dataset]. Data Publisher of Museum für Naturkunde Berlin (MfN) - Leibniz Institute for Evolution
and Biodiversity Science. https://doi.org/10.7479/87tp-gr35.
Talks, abstracts, and posters presentations
Pitriana, P. 2017. Barnacles on the coast of Ambon Island: Pasca Siboga Expedition 1899. Poster
presentation and Abstract Proceeding in SAGE 2017 – the 3rd Southeast Asian Gateway Evolution
Meeting: p.169.
Pitriana, P. 2018. Barnacles on the coast of Ambon Island: Pasca Siboga Expedition 1899. Talk
presentation in 19th Annual Meeting of the Gesellschaft für Biologische Systematik (GfBS), Vienna,
Austria: 11th-14th February 2018.
Pitriana, P. 2018. Integrative taxonomy, phylogeny and biogeography of barnacles (Crustacea: Cirripedia)
from the Spice Islands, Indonesia. Talk presentation in Evolutionary Biology Seminar Series at
Museum für Naturkunde Berlin, 1st November 2018.
Pitriana, P. 2020. Past, present and future: Integrative taxonomy of barnacles (Crustacea: Cirripedia)
from eastern Indonesia. Talk presentation in 21st Annual Meeting of the Gesellschaft für
Biologische Systematik (GfBS), Hamburg, Germany: 12nd-15th February 2020.
Pitriana, P. 2020. Biodiversity discovery of barnacles Acasta sulcata (Crustacea: Cirripedia) from the
Moluccas, eastern Indonesia. Talk presentation in the Webinar of East Indonesian seas as a global
epicentre of marine biodiversity, 6th August 2020. The video availabe online at https://www.
youtube.com/watch?v=6C_teRcVLmg.
Participation in courses, trainings, workshops, seminars, symposiums,
and interviews
•
•
•
Göttingen SPIRIT Summer School “Molecular Genetics for Zoologists”, in Göttingen, Germany (13
– 18 August 2017).
IOC-WESTPAC Training Course on “Molecular Taxonomy based on Single DNA Barcoding and
Metabarcoding”, in Jakarta and Pari Island, Indonesia (25 September – 5 October 2017).
59th Phylogenetisches Symposium “Phylogeny in The Post-Genomic Era”, in Berlin, Germany (17 –
19 November 2017).
242
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Chapter 7
Course of Molecular Phylogenies for Biodiversity, in Berlin, Germany (9 – 13 April 2018).
Intensive course and training of “Molecular Diagnostics for Species Identification and Evolutionary
Analysis”, in The Natural History Museum London, UK (13 – 19 May 2018).
Workshop of “Good Scientific Practice”, in MfN Berlin, Germany (3 – 4 July 2018).
Workshop of “Introduction and practice of Amira/3D”, in MfN Berlin, Germany (4 – 5 September
2018).
“Data Management Training” in Botanic Garden & Botanical Museum (BGBM), Berlin, Germany
(25 – 26 September 2018).
Course of “The First Aid for Fieldworkers” in Wittenberg, Germany (2 – 4 November 2018).
“Global Summit of Research Museums” in Berlin, Germany (4 – 6 November 2018).
Distributed European School of Taxonomy (DEST): “Integrative taxonomy course in the big data
era” at the Muséum National d’Histoire Naturelle, Paris, France (18 – 22 April 2019).
Workshop “Imaging with Adobe Photoshop - Professional Image Editing and Retauching” in Berlin,
Germany (27 – 28 May 2019).
Symposium of “Imaging technology: Advances in CT Technology for Lifescience and Natural History
Science Museum Collections”, in Berlin, Germany (12 – 14 June 2019).
Workshop “Scientific writing” in Berlin, Germany (7 – 8 August 2019).
Basic Collection Techniques Workshop at the Museum fur Naturkunde Berlin (25 – 26 November
2019).
Workshop of Centre for Integartive Biodiversity Discovery at the Museum fur Naturkunde Berlin,
Germany (27 – 28 November 2019).
Interviewed by Science Newsletter of MfN Berlin in December 2019. “The barnacles of eastern
Indonesia”, the article available online at
https://www.museumfuernaturkunde.berlin/en/science/barnacles-eastern-indonesia.
Interviewed by Deutsche Welle (DW) Indonesia. 2019. “Teritip: Terkenal Sebagai Hama Tapi
Menyimpan Banyak Potensi”, the article is in Bahasa and available online at https://www.dw.com/
id/teritip-terkenal-sebagai-hama-tapi-menyimpan-banyak-potensi/a-52038998.
Interviewed by Deutsche Welle (DW) Indonesia. 2019. “Menguak Potensi Hewan Laut Teritip
Melalui Taksonomi Integratif”, the article is in Bahasa and available online at https://www.dw.com/
id/menguak-potensi-hewan-laut-teritip-melalui-taksonomi-integratif/g-52038787.
Filmed by Deutsche Welle (DW) Indonesia. 2020. “Teritip Laut Harta Terpendam Kaya Manfaat”,
the film is in Bahasa and available online at https://www.youtube.com/watch?v=QB8_3L7Ihu4.
Appendix
243
7. 4 Curriculum vitae
For reasons of data protection, the curriculum vitae is not published in the electronic version.
244
Chapter 7
For reasons of data protection, the curriculum vitae is not published in the electronic version.
Appendix
245
7. 5 Declaration of originality
Berlin, September 2020
Hereby I declare that I wrote this doctoral thesis by myself without sources other than those
indicated in the text and related reference list. The work has not been previously submitted to
the Freie Universität Berlin or any other university.
_____________________________
Pipit Pitriana
246
Chapter 7
7. 6 Statement from proof reader
For reasons of data protection, the statement from proof reader is not published in the
electronic version.