Hostname: page-component-848d4c4894-x24gv Total loading time: 0 Render date: 2024-05-11T14:20:11.427Z Has data issue: false hasContentIssue false
  • English
  • Français

Significance of Achromobacter nematophilus Poinar and Thomas (Achromobacteraceae: Eubacteriales) in the development of the nematode, DD-136 (Neoaplectana sp. Steinernematidae)

Published online by Cambridge University Press:  06 April 2009

George O. Poinar Jr  and
Gerard M. Thomas
George O. Poinar Jr
Affiliation:
Division of Invertebrate Pathology, University of California, Berkeley, California
Gerard M. Thomas
Affiliation:
Division of Invertebrate Pathology, University of California, Berkeley, California
Get access Rights & Permissions [Opens in a new window]

Extract

By culturing Galleria mellonella, Neoaplectana sp. (DD-136) and Achromobacter nematophilus separately under axenic conditions in the laboratory, it was possible to study the relationship between the bacterium and nematode during nematode parasitism of the insect.

The infective-stage juveniles of the nematode were able to penetrate and kill the insect host without the presence of A. nematophilus or any other bacterium. However, without accompanying bacteria the nematode was unable to reproduce. Only when A. nematophilus or a possible replacement, such as Pseudomonas aeruginosa, was added to the blood did reproduction occur.

The relationship between A. nematophilus and the nematode is considered a mutualistic one, since the bacterium lives and is protected inside the intestine of the free-living stage of the nematode and is transported and released by the nematode to the haemolymph of a host insect. The nematode, in turn, is dependent on the bacterium for reproduction.

Type
Research Article
Information
Parasitology , Volume 56 , Issue 2 , May 1966 , pp. 385 - 390
Copyright
Copyright © Cambridge University Press 1966

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anonymous (1955). Nematode-borne disease that attacks insects is discovered by USDA scientist. U.S.D.A. Press Release no. 3216.Google Scholar
Dougherty, E. C. & Calhoun, H. G. (1948). Techniques for temporarily freeing soil nematodes from bacteria by the use of antibiotics and merthiolate. Anat. Rec. 100, 395.Google ScholarPubMed
Dutky, S. R. (1959). Insect microbiology. Adv. appl. Microbiol. 1, 175–200.CrossRefGoogle ScholarPubMed
Dutky, S. R. & Hough, W. S. (1955). Note on a parasitic nematode from codling moth larvae, Carpocapsa pomonella. Proc. ent. Soc. Wash. 57, 244.Google Scholar
Glaser, R. W. & Stoll, N. R. (1940). Exsheathing and sterilizing infective nematode larvae. J. Parasit. 26, 87–94.CrossRefGoogle Scholar
Glaser, R. W., McCoy, E. E. & Girth, H. B. (1942). The biology and culture of Neoaplectana chresima, a new nematode parasitic in insects. J. Parasit. 28, 123–6.CrossRefGoogle Scholar
Poinar, G. O. Jr. (1966). The presence of Achromobacter nematophilus Poinar and Thomas in the infective stage of a Neoplectana sp. (Steinernematidae: Nematoda). Nematologica (In the Press.)Google Scholar
Poinar, G. O. Jr. & Thomas, G. M. (1965). A new bacterium, Achromobacter nematophilus sp.nov. (Achromobacteraceae: Eubacteriales) associated with a nematode. Int. Bull. bact. Nomencl. Taxon. 15, (4), 249–52.Google Scholar