› 6da7

X-ray diffraction
1.83Å resolution

Crystal structure of the TtnD decarboxylase from the tautomycetin biosynthesis pathway of Streptomyces griseochromogenes with apo form at 1.83 A resolution (I222)

Released:
DOI: 10.2210/pdb6da7/pdb
PDB entry 6da7 coloured by chain, front view.
PDB entry 6da7 coloured by chain, side view.
PDB entry 6da7 coloured by chain, top view.
Source organism: Streptomyces griseochromogenes
Primary publication:
Biochemical and Structural Characterization of TtnD, a Prenylated FMN-Dependent Decarboxylase from the Tautomycetin Biosynthetic Pathway.
Annaval T, Han L, Rudolf JD, Xie G, Yang D, Chang CY, Ma M, Crnovcic I, Miller MD, Soman J, Xu W, Phillips GN, Shen B
ACS Chem Biol 13 2728-2738 (2018)
PMID: 30152678

Function and Biology Details

Reaction catalysed: 
Pyrrole-2-carboxylate + H(2)O = pyrrole + HCO(3)(-)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111664 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrrole-2-carboxylic acid decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 54.78 KDa
Source organism: Streptomyces griseochromogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C6ZCR8 (Residues: 1-485; Coverage: 100%)
Gene name: ttnD
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand EPE 1 x EPE
Ligand GOL 18 x GOL
Ligand UNL 1 x UNL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I222
Unit cell:
a: 52.352Å b: 114.387Å c: 194.351Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.213
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Bacterial terpenome.
Rudolf et al. (2021)
2 more