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PHEROMONES
INTRODUCTION
Semiochemicals
Pheromones Allelochemicals
Semiochemicals (GK. Semeon-mark or signal)
Pheromones ( GK. Pherein-to carry and hormon-to exite)
Allelochemicals (GK. Allelon of one another)
PHEROMONES
 Also called as ecto hormones.
 Chemical messengers that are transported outside the
body that have the potential to evoke physiological or
behavioral changes in another individual of the same
species.
 Derived from greek words pherein means to transfer and
hormon means to excite.
WHAT ARE THE MESSAGES FOR ?
 Finding a male
 Taking advantage of food resources –Aggregation
 Defence against predation
 Social behavior
THE FIRST ISOLATED PHEROMONE - BOMBYKOL
OH
Produced by the female silk worm moth Bombyx mori.
Structure elucidation by Butendant and co workers in1959.
CRYSTAL STRUCTURE OF PBP-BOMBYKOL
The 15.9 kDa PBP has approximate
dimensions of 40 x 35 x 30 Å.
BMPBP BINDING POCKET
 Bombykol is found in the large flask shaped cavity with a
tiny opening at the surface.
 The only part of bombykol that is not surrounded by
alpha helices is the hydroxyl end.
BOMBYKOL-BINDING AND RELEASE
 BmPBP undergoes a pH dependant conformational
transition.
 BmPBP does not bind ligands below pH-5.
 It undergoes a conformational change when mixed with
model proteins.
CLASSIFICATION
 Aggregation pheromones
 Alarm pheromones
 Signal pheromones
 Trail pheromones
 Territorial pheromones
 Information pheromones
 Releaser pheromones
 Sex pheromones
TYPES
i. Aggregation
A group of individuals
at one location is referred as
aggregation .
O
O
O O
1
4
5
7
1
4
5
7
striped ambrosia beetle
Aggregation of bug nymphs
Aggregation pheromone of female ambrosia
beetle Lineatin
CONTINUED…
ii. Alarm - Some species release a
volatile substance when attacked
by a predator that can trigger flight
or aggression in members of the
same species. For ex. Minnows
and Catfish release alarm pheromone
called Schreckstoff.
iii. Signal – It cause short term changes such as the
neurotransmitter release that activates a response.
CONTINUED...
iv. Trail – Trail pheromones are
common in social insects. For ex:
ants make their paths with these
pheromones which are volatile
hydrocarbons.
v. Territorial – These mark the
boundaries of an organisms territory in
cats and dogs, these hormones are
present in the urine which they deposit
on landmarks serving to make the
perimeter of the claimed territory.
CONTINUED…
vi. Information – These pheromones are indicative of an
animals identity a territory.
vii. Releaser – These cause an alteration in the behavior of
the recipient.
For ex: some organisms use powerful attractant
molecules to attract mates from a distance of two miles
or more.
CONTINUED…
viii. Sex pheromones
• In animals sex pheromones indicate the availability of
the female for breeding.
• Among EK organisms it promote sexual interaction in
numerous species.
• Many insects species such as the ant release sex
hormones to attract a mate.
(+)-grandisol
HO
sex hormone of male cotton bell weevil
Male insect antennae:
specialized to detect
female pheromones
Pheromone receptor (human)
bombykol
bombykal
=come to me
=go away
Structurally related pheromone
displaying distinguish behavioral
changes
WHERE DO PHEROMONES COME FROM?
 Urine
 Sweat
 Tears
WHO USES THEM ?
 Insects – Moth
 Other Invertebrates – spider ,ants
 Vertebrates – Mice ,Goldfish
 Humans
 Other animals like cats ,dogs etc.
FORAGING - IMPORTANT RED FIRE ANT
( SOLENOPIS INVICITA)
 Uses a complex trait pheromone in order to find food.
 Releases pheromone to mark the trail back to the nest.
 Other ants find the trial and follow it to the food source.
 Recruited ants leave a trail signal of their own.
PREY CAPTURE
 Some animals mimic
pheromone to trick prey.
 Bolas Spider mimics the
mating pheromone of the
moth.
 When the male moth
searches for a suitable
female.
MATING
 Goldfish have no defined territories.
 Males fight in ages to get close enough
to fertilize the females eggs.
 Female gold fish release two sets of pheromones:
preovulatoy and postovulatory.
 Male Goldfish can discriminate among the steroid
components of preovulatory pheromones.
SOCIAL BEHAVIOR
Mice use pheromones for social and reproductive
behavior.
MUPS
o Pheromones are excreted in urine from mice found to Major Urinary
Proteins.
o MUPs are a group of semiochemical binding proteins that carry the
mouse pheromones.
o All parts of the MUP are considered to have pheromonal affects.
o The MUP is made of a small protein and a ligand with room in the
middle for the pheromone molecule.
VNO (VOMERONASAL ORGAN )
 VNO contain receptor sites to detect pheromones.
 Found in the nasal cavities of mice and many other
animals.
 VNO is anatomically and functionally distant from the
olfactory system.
SIGNIFICANCE
 A pheromone trap can be used to trap pest for
monitoring purposes, to control the population by
creating confusion, to disrupt mating.
 Avoidance of inbreeding.
 Traps containing pheromones are used by farmers
to detect and monitor insect population in orchards.
DO HUMAN HAVE PHEROMONES?
Human pheromones are derived from axillary sweat and
tears reportedly control ovulation, mood, hormonal
secretion and attraction to genetically dissimilar mates .
As of now existence of human pheromone remain
controversial.
CONCLUSION
 Pheromones have a role in supporting reproductive, thus
increasing the chances for successful mating.
 Pheromones are not the only factors. Other sensory
factors, memory and learning co-integrate to ensure the
success of reproduction.
 Pheromones work in accordance with the type of social
organization.
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Pheromones.pptx

  • 2. INTRODUCTION Semiochemicals Pheromones Allelochemicals Semiochemicals (GK. Semeon-mark or signal) Pheromones ( GK. Pherein-to carry and hormon-to exite) Allelochemicals (GK. Allelon of one another)
  • 3. PHEROMONES  Also called as ecto hormones.  Chemical messengers that are transported outside the body that have the potential to evoke physiological or behavioral changes in another individual of the same species.  Derived from greek words pherein means to transfer and hormon means to excite.
  • 4. WHAT ARE THE MESSAGES FOR ?  Finding a male  Taking advantage of food resources –Aggregation  Defence against predation  Social behavior
  • 5. THE FIRST ISOLATED PHEROMONE - BOMBYKOL OH Produced by the female silk worm moth Bombyx mori. Structure elucidation by Butendant and co workers in1959.
  • 6. CRYSTAL STRUCTURE OF PBP-BOMBYKOL The 15.9 kDa PBP has approximate dimensions of 40 x 35 x 30 Å.
  • 7. BMPBP BINDING POCKET  Bombykol is found in the large flask shaped cavity with a tiny opening at the surface.  The only part of bombykol that is not surrounded by alpha helices is the hydroxyl end.
  • 8. BOMBYKOL-BINDING AND RELEASE  BmPBP undergoes a pH dependant conformational transition.  BmPBP does not bind ligands below pH-5.  It undergoes a conformational change when mixed with model proteins.
  • 9. CLASSIFICATION  Aggregation pheromones  Alarm pheromones  Signal pheromones  Trail pheromones  Territorial pheromones  Information pheromones  Releaser pheromones  Sex pheromones
  • 10. TYPES i. Aggregation A group of individuals at one location is referred as aggregation . O O O O 1 4 5 7 1 4 5 7 striped ambrosia beetle Aggregation of bug nymphs Aggregation pheromone of female ambrosia beetle Lineatin
  • 11. CONTINUED… ii. Alarm - Some species release a volatile substance when attacked by a predator that can trigger flight or aggression in members of the same species. For ex. Minnows and Catfish release alarm pheromone called Schreckstoff. iii. Signal – It cause short term changes such as the neurotransmitter release that activates a response.
  • 12. CONTINUED... iv. Trail – Trail pheromones are common in social insects. For ex: ants make their paths with these pheromones which are volatile hydrocarbons. v. Territorial – These mark the boundaries of an organisms territory in cats and dogs, these hormones are present in the urine which they deposit on landmarks serving to make the perimeter of the claimed territory.
  • 13. CONTINUED… vi. Information – These pheromones are indicative of an animals identity a territory. vii. Releaser – These cause an alteration in the behavior of the recipient. For ex: some organisms use powerful attractant molecules to attract mates from a distance of two miles or more.
  • 14. CONTINUED… viii. Sex pheromones • In animals sex pheromones indicate the availability of the female for breeding. • Among EK organisms it promote sexual interaction in numerous species. • Many insects species such as the ant release sex hormones to attract a mate. (+)-grandisol HO sex hormone of male cotton bell weevil
  • 15. Male insect antennae: specialized to detect female pheromones
  • 17. bombykol bombykal =come to me =go away Structurally related pheromone displaying distinguish behavioral changes
  • 18. WHERE DO PHEROMONES COME FROM?  Urine  Sweat  Tears
  • 19. WHO USES THEM ?  Insects – Moth  Other Invertebrates – spider ,ants  Vertebrates – Mice ,Goldfish  Humans  Other animals like cats ,dogs etc.
  • 20. FORAGING - IMPORTANT RED FIRE ANT ( SOLENOPIS INVICITA)  Uses a complex trait pheromone in order to find food.  Releases pheromone to mark the trail back to the nest.  Other ants find the trial and follow it to the food source.  Recruited ants leave a trail signal of their own.
  • 21. PREY CAPTURE  Some animals mimic pheromone to trick prey.  Bolas Spider mimics the mating pheromone of the moth.  When the male moth searches for a suitable female.
  • 22. MATING  Goldfish have no defined territories.  Males fight in ages to get close enough to fertilize the females eggs.  Female gold fish release two sets of pheromones: preovulatoy and postovulatory.  Male Goldfish can discriminate among the steroid components of preovulatory pheromones.
  • 23. SOCIAL BEHAVIOR Mice use pheromones for social and reproductive behavior.
  • 24. MUPS o Pheromones are excreted in urine from mice found to Major Urinary Proteins. o MUPs are a group of semiochemical binding proteins that carry the mouse pheromones. o All parts of the MUP are considered to have pheromonal affects. o The MUP is made of a small protein and a ligand with room in the middle for the pheromone molecule.
  • 25. VNO (VOMERONASAL ORGAN )  VNO contain receptor sites to detect pheromones.  Found in the nasal cavities of mice and many other animals.  VNO is anatomically and functionally distant from the olfactory system.
  • 26. SIGNIFICANCE  A pheromone trap can be used to trap pest for monitoring purposes, to control the population by creating confusion, to disrupt mating.  Avoidance of inbreeding.  Traps containing pheromones are used by farmers to detect and monitor insect population in orchards.
  • 27. DO HUMAN HAVE PHEROMONES? Human pheromones are derived from axillary sweat and tears reportedly control ovulation, mood, hormonal secretion and attraction to genetically dissimilar mates . As of now existence of human pheromone remain controversial.
  • 28. CONCLUSION  Pheromones have a role in supporting reproductive, thus increasing the chances for successful mating.  Pheromones are not the only factors. Other sensory factors, memory and learning co-integrate to ensure the success of reproduction.  Pheromones work in accordance with the type of social organization.
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