RMRGJR72–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Photograph 2.—Bird No. I—Non-infected Hen; Bird No. II—Infected Hen. Pulloruni diseased birds as a rule cannot be differentiated from the non-infected by physical examination. Apparently normal, well-developed birds may be affected with the disease. Infection in such birds can be detected either by the agglutination test or by bacteriological examination. Refer to questions 4 and 33.. Photograph 3—Ovaries Removed from Hens. Specimens I and HI, showing irregular shaped, discolored and pedunculated ova, are
RMRE46R4–. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. BACILLUS COLI COMMUNIS 397 specificity of the agglutination reaction. Thus a serum which will agglutinate its homologous strains in dilutions of one 1:1,000 will often fail to agglutinate other races of Bacillus coli in dilutions of 1:500 or 1 : 600. The normal serum of adult animals and man will often agglutinate this bacillus in dilutions as high as 1 : 10 or 1 : 20—a phenomenon pos- sibly referable to its habitual presence within the body. Corrobo- rating this assumption is the obse
RMRHYNK2–. The beginnings of embryonic development : A symposium organized by the Section on Zoological Sciences of the American Association for the Advancement of Science, cosponsored by the American Society of Zoologists and the Association of Southeastern Biologists, and presented at the Atlanta meeting, December 27, 1955. Embryology. Fig. 1. (a) Agglutination of sperm by interaction of multivalent ferti- lizin with antifertilizin of sperm surface, (b) Multivalent fertilizin split into univalent fragments, (c) Failure of sperm to agglutinate with multivalent fertilizin following saturation with univ
RMRHP72B–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIG. 30. The agglutination cycle when sperm alone are aged and when the sperm are ripe at the time of shedding. This figure shows the marked, progressive, but slower increase in agglutinability with age. FIG. 3&. The curve for ageing sperm, when the sperm is moderately overripe when shed, showing the precocious and large increase in agglutinability, and the very rapid decrease thereafter. FIG. 3c. The agglutination cycle for ageing sperm, when the sperm were more overripe at the time of shedding. This shows a progressive
RMRE22E6–. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. IMMUNITY AND IMMUNIZING AGENTS. 123 tion of the precipitate has been termed precipitin. Similar reactions are observed with milk and egg albumen, when used with their specific immune sera. These reactions have been utilized to secure evidence in crimina cases. The serum of an animal which has been injected with human blood (humanized immune serum) produces a precipitate when mixed with human blood, even in high dilutions. Like agglutination, the reaction is, however, not wholly specific. For
RMREKJPX–. Clinical bacteriology and vaccine therapy for veterinary surgeons. Veterinary bacteriology; Vaccines. Fig. 29.—Agglutination of Bacteria (Jowett) A, Bacteria evenly distributed ; B, Bacteria agglutinated by own ends—a very improbable presumption. Allen is of opinion, however, the bacteria are gathered into clumps in the manner just described, so that they may be caught up in the various tissues, such as the liver, the spleen, and lymphatics, where the immunizing bodies are generated, and their destruction thereby brought about. Widal first suggested using this agglutinating power for diagnos
RMRHP71Y–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIG. 30. The agglutination cycle when sperm alone are aged and when the sperm are ripe at the time of shedding. This figure shows the marked, progressive, but slower increase in agglutinability with age. FIG. 3&. The curve for ageing sperm, when the sperm is moderately overripe when shed, showing the precocious and large increase in agglutinability, and the very rapid decrease thereafter. FIG. 3c. The agglutination cycle for ageing sperm, when the sperm were more overripe at the time of shedding. This shows a progressive
RMRJNC2J–. Bacteriology and the public health. Bacteriology; Public health. ' - * Bacillus typhosus, showing fiagella. x 1000. 6. Widal-Gruber Reaction. Agglutination of /.'. typhosus by blood serum of a typhoid patient. X 400. :v *c ****,'/ *-â >$. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Newman, George, Sir, 1870-1948. Philadelphia : P. Blakiston
RMRJNFNY–. Bacteria, especially as they are related to the economy of nature. Bacteriology. « Bacillus Typhosis (Showing Flagella) Bacillus Typhosus (Widal Reaction) X looo (Agglutination by serum from typhoid patient) X 400. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Newman, George, Sir, 1870- [from old catalog]. New York, G. P. Putnam's sons;
RMRGJPY3–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. PILLORI'M DISEASE 15. Photograph 14.—The Rapid Serum Agglutination Test. The mixtures in the squares on the left show a negative reaction, and the mixtures in the squares on the right show a positive reaction. Refer to question 39.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Massachusetts Agricultural Experiment Station. Amherst,
RMRE46W3–. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. Fig. 59.—Microscopic Agglutination Reaction. bearing on bacteriological species differentiation. Since animals im- munized against a definite species of bacteria acquire in their sera specific agglutinating powers for these bacteria and at best only slight agglutinating powers for other species, immune sera can be used ex- tensively in differentiating between bacterial varieties. Agglutination may be observed microscopically or macroscopically. Bacteria brought into contact with agglut
RMRHP978–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Vol. LXXVIII, No. 2 THE April, 1940 BIOLOGICAL BULLETIN PUBLISHED BY THE MARINE BIOLOGICAL LABORATORY SPERM AGGLUTINATION IN THE KEYHOLE LIMPET, MEGATHURACRENULATA ALBERT TYLER (From the William G. Kerckhoff Laboratories of the Biological Sciences, California Institute of Technology, Pasadena) F. R. Lillie (1913) demonstrated that the sea water in which ripe sea-urchin eggs have been standing acquires the property of causing an agglutination of the spermatozoa of the same species. This iso- agglutination of sperm by egg wate
RMREYJXT–. The pathology and differential diagnosis of infectious diseases of animals. Veterinary medicine -- Diagnosis; Communicable diseases in animals. 554 IMMUNITY power of clumping red blood corpuscles. This clumping or so-called agglutination of the red cells occurs previous to their. Fig. 122. ,/, Co)iip/e- metit; b, iiitcruiediary body; c, receptor; cf, part of the cell.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Moore
RMRGMMA4–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Figure 18. The^Whole Blood Agglutination Test. The'mixtures in the squares on the left show a negative reaction, and the mixtures on the right a'positive reaction. Refer to question 40.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Massachusetts Agricultural Experiment Station. Amherst, : Massachusetts Agricultural Experiment Statio
RMRGKK26–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. -MASS. EXPERIMENT STATION BULLETIN 323. Figure 2. An Electrically Heated and Lighted Apparatus Used for the Whole Blood Agglutination Test. Size 15 by 15 inches, by 14 inches deep. Construction consists of a wooden frame lined with Celotex and metal.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Massachusetts Agricultural Experiment
RMRGJPXC–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Photograph 15.—The Whole Blood Agglutination Test. The mixture in the squares on the left shows a negative reaction, and mixtures on the right show a positive reaction. Refer to question 40.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Massachusetts Agricultural Experiment Station. Amherst, : Massachusetts Agricultural Experiment S
RMRR3089–. [Publications] - Bureau of Government Laboratories. Biology; Medicine; Natural history. AUylulination.—$i>'L>xa observers luive noted the bunching to«rpthcr of Trypanosoma under certain conditions, and have described the phe- nomenon us agglutination. Some, as has already been mentioned, con- sider this to he a .multiplication phase, while others suppose if t«» he the natural position assumed by the parasites ju^t before dying. The process lias not been seen at all by Katie of I he most careful investigators. Lavemn and Mesivil regard the agglutination of: Trypanosoma as a brought ab
RMRGJPXK–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Photograph 14.—The Rapid Serum Agglutination Test. The mixtures in the squares on the left show a negative reaction, and the mixtures in the squares on the right show a positive reaction. Refer to question 39.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Massachusetts Agricultural Experiment Station. Amherst, : Massachusetts Agricu
RMRHP96M–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 162 ALBERT TYLER of initial agglutinates decreases and they fail to fuse into a single clump. Finally a dilution is reached at which no visible agglutination occurs. The time at which agglutination first becomes visible also varies with the strength of the egg water and the sperm suspension as will be shown below.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original
RMRHP8WD–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SPERM AGGLUTINATION 173 As before, the agglutinin titer (the time for visible agglutination de- creases with increase in agglutinin concentration as shown in Table I) of the supernatant sea water increases with the time that the eggs remain in contact with it. There is, however, no corresponding increase in the amount of agglutinin that can be obtained after adding the acid. This remains essentially constant showing again no production of agglutinin by the eggs. While the above evidence locates the agglutinin in the jelly la
RMRGN5T0–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture. MAP OF THE STATE OF MASSACHUSETTS. SHOWING ^REM, IN WlilCll INFECTION HAS BEEN DETECTED UV THE ISOLATION Or Cl.l.n;RES OK liACTERIUM Pll.LORl'M ANI> THE AGGLUTINATION TEST.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Massachusetts Agricultural Experiment Station. Amherst, : Massachusetts Agricultural Experiment Station, 1907-1974
RMRR30W8–. The actinomycetes: their nature, occurrence, activities, and importance. Actinomyces; Actinomycetales. Chapter I —21— Taxonomy reaction corresponded well to the agglutination reaction. The agglutinating re- ceptors were present more abundantly in the spores than in the mycelium. V. Magnus (282) has been able to separate various strains of actinomycetes, on a serological basis into acid producers, alkali producers and neutrals; hemoagglutina- tion phenomena were found to occur among 80 per cent of tiie acid producers. 8. Phage specificity. Certain actinomycetes are subject to attack by specif
RMRGMM9C–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Figure 20. An Electrically Heated and Lighited Apparatus Used for the Whole Blood Agglutination Test. Size 15 by 1.5 inches, by 14 inches deep. Construction consists of a wooden frame lined with Cclotex and metal. Refer to question 40.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Massachusetts Agricultural Experiment Station. Amher
RMRHJR0W–. Biology; the story of living things. â ^/////////,y.//^,^M,//y/////yl4^. Agglutination test for typhoid. The diagram at the left shows free-swimming bacteria, at the right the bacteria have become clumped together by theagglutins produced by the body cells. the antibodies are already formed and are at work. Just as each disease is caused by a specific kind of organism producing a specific type of toxin, so the blood forms a specific type of antibody for each toxin. Another method of receiving immunity has been recently discovered independently by two investigators, Twort and d'Herelle. The l
RMRGJPYH–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. 14 MASS. EXPERIMENT STATION BULLETIN No. 284 liicul)ator (iisiiifoction should he regarded as part of the general sanitary program and not as a disease eradication measure in its(>lf. 35.—How may carriers of this disease be detected.' Infeeted hirtls may be detected by the following tests: Intradermal test; macroscopic agglutination test; rapid serum agglutination test; and whole blood agglutination test. 36.—What is the intradermal, pullorin, or "wattle" test? This test consists of injecting
RMRE53BH–. Special pathology and therapeutics of the diseases of domestic animals. Veterinary medicine. Sero-Diagnosis and Allergic leactiou. 747 Sero-Diagnosis and Allergic Reaction. Recent investiga- tions permit tlie assumption that it will be possible to deter- mine infection with the bacillus of abortion by bacteriological methods long before and after the abortion. If these results are confirmed the com- batting of the disease will appear con- siderably more effective, as it will be possible to prevent the introduction of the virus by apparently healthy ani- mals. 1. Agglutination Test. Kxperime
RMRHYNFA–. The beginnings of embryonic development : A symposium organized by the Section on Zoological Sciences of the American Association for the Advancement of Science, cosponsored by the American Society of Zoologists and the Association of Southeastern Biologists, and presented at the Atlanta meeting, December 27, 1955. Embryology. A. L. COLWIN AND L. H. COLWIN 141. Figs. 16-18. Electron micrographs of spermatozoa of the sea urchin, Strongylocentrotus pulcherrimus. (From Dan, Biol. Bull., 1952.) 16, after agglutination with egg'water. 17, after treatment with alkaline sea water. 18, in sea water
RMREJCJM–. Colchicine in agriculture, medicine, biology, and chemistry. Colchicine; Colchicine. Fig. 2.8—Stages of restitution in exploded metaphases in the regenerating liver of rats injected with colchicine. Feulgen-fast green staining. A. Eight hours after colchicine. Typical exploded metaphase, without spindle. Scattered and shortened chromosomes. B. Sixteen hours. Chromosome agglutination and lengthening. C. Sixteen hours. Some sug- gestion of catachromatic changes. D. Thirty hours. Formation of large micronuclei; these originate by the catachromatic changes of agglutinated groups of chromosomes.
RMRHP58W–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ULTRAVIOLET AND ARBACIA FERTILIZATION. 429 of the onset of agglutination to he a function of sperm activity. The time during which the agglutinated masses remain intact also (See Fig. 3.) decreases with increased dosage. See-.. FIG. 3. Delay in agglutinating reaction following radiation of sperm. Ord:nates, number of seconds elapsed before the onset of agglutination with normal egg-water. Abscissae, length of period of exposure, in seconds. The same conditions are found when egg-water is radiated for a short interval, and th
RMRHP70J–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. <?))( JTI nouri. FIG. 50. The agglutination changes when both the germ cells are ripe at the time of shedding and the same germ cells used at successive ages. The graphs strongly suggest the large influence of the sperm. Note the marked increase in agglutination. FIG. 5c. When eggs were overripe at the time of shedding no marked nor pro- gressive increase occurred, due to insufficient agglutinin.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - c
RMRGMMR3–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. /yV /A/CUBATOff Cycle or Infection. CHICKS //Y B/fOOOEf? /iOUOE Figure 1. Pullorum Disease Infection in a Floclt May Follow This Cycle. The disease can be eradicated either by breaking the cycle of infection through the elimination of the infected birds by means of the macroscopic agglutination test, or by disposing of the entire infected flock and replacing it with pullorum disease-free stock. 8. What is a pullorum disease "carrier"? A "carrier" is an infected bird that did not succum
RMRJD4P5–. The cell and protoplasm ... Cells; Protoplasm; Cytology; Cytoplasm. STRUCTUHAL UNITS IN t'ELLlTLAK PHYSIOLOGY 201 has a minimum at a definite distance of equilibrium. Tlie ]iliysieal si<iiiificaiice of this equilibrium depends, however, on the energy inyolved. For two spherical i)ar- ticles of the dimensions of a large protein molecule, diameter 100 A, this minimum is of the order of 0.1 of the thermal energy, and consequently no e(inilil)rium results, the total etfect of the forces being to pre- vent agglutination. The situation is very different, however, with larger particles and espec
RMRT05EC–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 492 RALPH R. HATHAWAY Species specificity of respiratory activators Although the agglutination of spermatozoa by egg water is highly specific (Lillie, 1919; Metz, 1945; Tyler, 1948), there has been no clear demonstration of specificity among activators of sperm motility and respiration. A specific activator was thought to occur in starfish egg water (Loeb, 1915), but it was later demon- strated that starfish spermatozoa are virtually immotile in both sea water and egg water (Metz, 1945). In the same paper, Loeb also reported
RMRDCJXY–. The farmer's veterinary adviser : a guide to the prevention and treatment of disease in domestic animals . Veterinary medicine. 27G The Farmer's Veterinary Adviser. Fig. 43.. Fig. 43—Hair with spores of Achorion Schonleini, from the horse.—Megnin DIFFUSE BALDNESS (TINEA DECALVANS). PAEASITIO PITYRIASIS Two other forms are seen in the horse, one attacking any part of the body, and recognized by the agglutination of five or six hairs together in a white crust, and the other attacking the heads of old horses, and characterized mainly by the scurfy product. Both are exceedingly inveterate, thoug
RMRE3H41–. Bacteriology and the public health. Bacteriology; Public health. PLATF. 20. -â ^ *JL //** '^ ^^ V^V ⢠) - - -^ ⢠â J:.-CiKvvKK Rkac;tion'. Agglutination uf 7.'. fiijih'isn^ by blood .serum of :i typliuiil liiiLi.-nt. X 400.. ..A u <-*^". Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Newman, George, Sir, 1870-1948. Philadelphia, P. Blakiston's Son & Co.
RMRHM6GA–. The Biological bulletin. Biology; Zoology; Marine biology. ANTIGENS OF SEA URCHIN SPERM SURFACE 103 sperm surface and fertilizin do not have antigenic combining sites in common which are essential for sperm agglutination. On the other hand, the combining sites of the soluble antigens in frozen-thawed extracts evidently are blocked by fertilizin. This is again seen in Figure 3, curve c. In this experiment decreasing amounts of fertilizin were mixed with increasing amounts of the sperm surface extract (antifertilizin). The mixtures were subsequently used to absorb the anti-sperm serum. When th
RMRE46TX–. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. 230 INFECTION AND IMMUNITY individual microorganisms become more and more scarce, finally leaving the medium between clumps entirely clear. While the clumping of a motile organism suggests that motility has something to do with the coming together in clumps, it nevertheless has no relation whatever to agglutination, motile and non-motile organisms alike being subject to the reaction. Macroscopically observed, in small test tubes or capillary tubes, ag- glutination evidences itself by t
RMRHP937–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SPERM AGGLUTINATION 163 clition of the sperm. In the following account an egg water will be designated as strong if, when mixed with an equal volume of a one per cent sperm suspension, it gives an agglutination reaction that is macro- m. f V m. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biolog
RMRDHJ53–. Microbes & toxins. Bacteriology; Toxins; Antitoxins. APPLICATIONS OF BACTERIOLOGY 253. Fig. 71.—Agglutination of the typhoid bacillus by the serum of a typhoid patient: b, clump of bacilli : g.s., blood corpuscles left in the serum. throughout the liquid; the suspension is "homogeneous." If a trace of serum is added from an animal prepared by injections of typhoid bacilli or from a patient suffering from typhoid fever, the bacilli lose their motility and collect into masses: they are said to become agglutinated by the serum. If the serum is added to a broth culture floccules ca
RMRDCJXW–. The farmer's veterinary adviser : a guide to the prevention and treatment of disease in domestic animals . Veterinary medicine. Fig. 43—Hair with spores of Achorion Schonleini, from the horse.—Megnin DIFFUSE BALDNESS (TINEA DECALVANS). PAEASITIO PITYRIASIS Two other forms are seen in the horse, one attacking any part of the body, and recognized by the agglutination of five or six hairs together in a white crust, and the other attacking the heads of old horses, and characterized mainly by the scurfy product. Both are exceedingly inveterate, though not attended with excessive itching, and dema
RMRHRKG2–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. THE PRODUCTION OF GRAFTED EMBRYOS. 85 The blastulae are particularly interesting for they make it possible to determine definitely whether the component members of a cluster are merely agglutinated or fused. Figs. 26, 27 and 36 37 38. 43 FIGS. 36-43. 28 represent half blastomeres in varying degrees of approximation or separation, with their blastocceles entirely separate. Figs. 29 and 30 represent an agglutination of a half and a whole egg,. Please note that these images are extracted from scanned page images that may have b
RMRE1D6E–. A manual of bacteriology. Bacteriology. 324 MANUAL OF BACTERIOLOGY. followed by considerable constitutional disturbance. This method seems likely to be of considerable value. Yersin and others have prepared protective sera on the same general principles used in making other sera for effecting pas- sive immunity. The results so far obtained with these sera are not especially encouraging.*. Fig. 77.—Bacillus aerogenes capsulatus, smear preparation from rabbit's liver.. (X 1000.) An agglutination reaction has been described; but this is not likely to be of great value in diagnosis. The period o
RMRE5587–. The farmer's veterinary adviser, a guide to the prevention and treatment of disease in domestic animals. Veterinary medicine; Veterinary hygiene. Fig. 43—Hair with spores of Achorion Schonleini, from the horse.—Megnin DEFFUSE BAIJ)NESS (TINEA DECALYANS). PAEASITIO PITYEIASIS Two other forms are seen in tlie horse, one attacldng any part of the body, and recognized by the agglutination of five or six hairs together iq a white crust, and the other attacking the heads of old horses, and characterized mainly by the scurfy product. Both are exceedingly inveterate, though not attended with excessi
RMRHP3JN–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 134 VANCE TARTAR AND TZE-TUAN CHEN In the reciprocal cross enucleate fragments of Grl4 were placed with whole animals of McD3. Such a mixture gave the same agglutination as described above. Twelve such fragments which reacted were stained and no trace of a nucleus or part of a nucleus was found in them.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Mar
RMRGKK1G–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Figure 2. An Electrically Heated and Lighted Apparatus Used for the Whole Blood Agglutination Test. Size 15 by 15 inches, by 14 inches deep. Construction consists of a wooden frame lined with Celotex and metal.. Figure 3. Same Apparatus with Cover Removed. The tesf plate si/c 12 by 12 inches) slides into a removable frame which is covered with glass.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of th
RMRGJR7R–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Photograph 2.—Bird No. I—Non-infected Hen; Bird No. II—Infected Hen. Pulloruni diseased birds as a rule cannot be differentiated from the non-infected by physical examination. Apparently normal, well-developed birds may be affected with the disease. Infection in such birds can be detected either by the agglutination test or by bacteriological examination. Refer to questions 4 and 33.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability
RMRG450N–. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. 144 CALIFORNIA FISH AND CAME agglutinated by rabbit £ ictaluri antiserum from Auburn University, and by rabbit antiserum we produced against the type strain ATCC 33202 (Hawke et al. 1981). No agglutination was observed when these isolates were mixed with antiserum to E. tarda. Dr. J. A. Plumb, Department of Fisheries and Allied Aquaculture, Auburn University, confirmed the isolate from fingerling channel catfish as £. ictaluri by bioc
RMRGMMNF–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Figure 3. Bird No. !—Noninfected Hen; Bird No. II—Infected Hen. Pullorum diseased birds as a rule cannot be differentiated from the noninfected by physical examination. Apparently normal, well-developed birds may be affected with the disease. In- fection in such birds can be detected either by the agglutination test or by bacteriological examin- ation. Refer to questions 4 and 33. /&• ' ^. / / /. Please note that these images are extracted from scanned page images that may have been digitally enhanced
RMRHP914–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SPERM AGGLUTINATION 165 can readily be found. However, the origin and behavior of such ag- glutinates indicate that only one kind of agglutinin is present in the original egg water.. m , a b. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1
RMRGMMNW–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Figure 3. Bird No. !—Noninfected Hen; Bird No. II—Infected Hen. Pullorum diseased birds as a rule cannot be differentiated from the noninfected by physical examination. Apparently normal, well-developed birds may be affected with the disease. In- fection in such birds can be detected either by the agglutination test or by bacteriological examin- ation. Refer to questions 4 and 33. /&• ' ^. Please note that these images are extracted from scanned page images that may have been digitally enhanced for re
RMRHMT7H–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 252 GREGOR T. POPA. urchin eggs have been for a short time. The reaction is in- stantaneous. The spermatozoa exhibit great activity and bunch together, for a while, in compact clusters of various sizes. What is the immediate cause of the agglutination residing in the spermatozoa? Is it due to a change in the arrangement of the substances, or to an active elimination of the sticky substance observed in fresh suspensions of spermatozoa?. FIG. 10. Arrangement of agglutination experiment; a, egg-water, b, clusters- c, sperm-susp
RMRHN2HF–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. D 2 £ £ i i Fraction numbei FIGURE 3. Bio-gel A5m elution patterns of Holothuria polii coelomic fluid from animals injected with phosphate buffered saline (a) or formalinized sheep erythrocytes (b). Bars indicate the distribution of hemolytic CH and hemagglutinating • activities against rabbit erythrocytes. The end liter was the reciprocal of the last dilution revealing a clear agglutination, or hemolysis (at least 10%). found only when the pooled fractions were concentrated by ultrafiltration to a fifth of their volume. The
RMRE99E2–. Control series bulletin. Feeds -- Analysis Periodicals; Fertilizers -- Analysis Periodicals. PULLLT NO.Z NON-INFECTED / -^ o -^ Pullet No. 1 was infected during chickhood, and has become a carrier of the dis- ease. It has laid an infective egg on the floor, and this egg was eaten by non-infected pullet No. 2.. Two months later both pullets were detected as carriers of pullorum disease by tihe agglutination test. Early pullet testing will permit elimination of birds such as pullet No. 1 and prevent spread of the disease by means of their eggs.. Please note that these images are extracted from
RMRJNFNW–. Bacteria, especially as they are related to the economy of nature. Bacteriology. « Bacillus Typhosis (Showing Flagella) Bacillus Typhosus (Widal Reaction) X looo (Agglutination by serum from typhoid patient) X 400. Bacillus Coli Communis (From agar culture, 48 hours growth) X 1000 /' ^ • ^ Bacillus Mycoides (Spore formation. From agar culture) X 1000 By permission 0/ the Scientific Press^ Limited. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resem
RMRE0H9E–. Diseases of poultry; their etiology, diagnosis, treatment, and prevention. Poultry. Tuberculosu- 127 recorded 24, 48 and 72 hours after the injection. A positive reaction is indicated in a typical case liy a large swelHng. Fig. 18. â Head of chicken showing positive tuberculin reaction of comb , and right wattle. (After Van Es and Schalk.) about the point of injection. In the wattle this organ often becomes two to three times its original thickness. Agglutination and complement fixation tests have also been used to diagnose this disease.^ The tests so far re- ' Himmelberger, L. G., loc. cit.
RMRHMRGF–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HETEROAGGLUTININS IN LOBSTER-BLOOD 195 centration (from Kjeldahl nitrogen determination) of the solution was 0.7 per cent and its titer (minimum dilution giving definite microscopic agglutination) was 128. A sample of serum containing 5 per cent protein gave at the same time a titer of 256. This preparation showed, then, about 3^/2 times the activity of the whole serum. Electrophoretic examination of the pale precipitate - Another sample of the material freed of hemocyanin was reprecipitated at pH 5, dissolved in dilute ammo
RMRHP2YD–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROPERTIES OF ARBACIA SPERM EXTRACTS 193 a homogeneous suspension which slowly settles (Fig. 1, C). Subse- quent agitation of each tube causes an increase in the density of the egg- masses in the extract, while the eggs in sea water redistribute them- selves through the solution and again settle to the bottom of the tube. We will call this phenomenon the " egg-agglutination reaction," and the inciting substance present in sperm extracts, the egg-agglutinating sub- stance. These terms are used in a purely descriptiv
RMRHP93Y–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIG. 1. Macroscopic appearance of the agglutination reaction in Megathura. Photographed in Syracuse dishes. X %. a, Untreated sperm suspension (ca. 2 per cent) ; b, 15 seconds after addition of egg water; c, 30 seconds; d, 10 minutes. The small agglutinates are typically spherical in shape. The larger ones are considerably flattened. The shape is similar to, although some- what flatter than, that assumed by a drop of mercury in a dish of water. The form of the agglutinates as seen under the microscope varies with the strengt
RMRHP707–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIG. 50. The agglutination changes when both the germ cells are ripe at the time of shedding and the same germ cells used at successive ages. The graphs strongly suggest the large influence of the sperm. Note the marked increase in agglutination. FIG. 5c. When eggs were overripe at the time of shedding no marked nor pro- gressive increase occurred, due to insufficient agglutinin.. + fa -t 20 o - ^o - to - 40 fo — too F.^.Ya. Please note that these images are extracted from scanned page images that may have been digitally e
RMRGMM90–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Figure 20. An Electrically Heated and Lighited Apparatus Used for the Whole Blood Agglutination Test. Size 15 by 1.5 inches, by 14 inches deep. Construction consists of a wooden frame lined with Cclotex and metal. Refer to question 40.. Figure 21. Same Apparatus with Cover Removed. The test plate (size 12 by 12 inches) slides into a removable frame which is covered with glass. Refer to question 40. (20). Please note that these images are extracted from scanned page images that may have been digitally enha
RMRHKYNA–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. STUDIES ON ACROSOME. II 209 other, and the tails in vigorous movement (Fig. 3). This agglutination is irre- versible. Many other spermatozoa are in rapid solitary motion, and some can he found with their heads stuck against the glass surfaces, while their tails move freely. Close examination shows that these spermatozoa are held affixed to the glass by a long (ca. 25/x), very slender filament which extends perpendicularly from the center of the acrosome surface (Figs. 3, 4 and 5a and b, 6). These filaments can- not be seen o
RMRE558B–. The farmer's veterinary adviser, a guide to the prevention and treatment of disease in domestic animals. Veterinary medicine; Veterinary hygiene. 368 The Farmer's Veterinary Adoiser. Fig. d3.. Fig. 43—Hair with spores of Achorion Schonleini, from the horse.—Megnin DEFFUSE BAIJ)NESS (TINEA DECALYANS). PAEASITIO PITYEIASIS Two other forms are seen in tlie horse, one attacldng any part of the body, and recognized by the agglutination of five or six hairs together iq a white crust, and the other attacking the heads of old horses, and characterized mainly by the scurfy product. Both are exceeding
RMRHRNJM–. Biochemical systematics. Biochemical variation; Botany. SEROLOGY AND SYSTEMATICS 85 One point raised previously is of particular interest. It was stated that simple sugars may sometimes inhibit specific plant agglutinins. The first report of inhibition of this type was that of Watkins and Morgan (1952) who, in this case using anti-H agglutinin of eel serum, discovered that agglutination was inhibited by L-fucose. In addition to certain simple sugars, the sugar derivation N-acetyl- galactosamme inhibits anti-A, and anti-B agglutinins from a number of sources (Makela, 1957). This observation a
RMRE99B2–. Control series bulletin. Feeds -- Analysis Periodicals; Fertilizers -- Analysis Periodicals. CHICKS W B/fOOOC/f HOUSE. Figure 1. Infection in a Flock May Follow This Cycle. The cycle of infection can best be broken by eliminating the infected birds from the young and adult stock by means of the agglutination test. In iuunature and adult birds, tlie disease may occasionally manifest itself in an acute form causing slight to heavy losses. Usually in diseased adults, the infection is localized in one or more organs such as the ovary, heart sac and heart wall, oviduct, and cysts, attached or fre
RMRGKK2N–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Figure 1. The Whole Blood Agglutination Test. The mixtures in the squares on the left show a negative reaction; the mixtures on the right show a positive reaction. TECHNIQUE OF TESTING METHODS USED Whole Blood Method. The antigen employed was prepared according to the procedure recommended by U. S. B. A. I., U. S. Patent No. 1,816,026, with a slight modification. The organisms were cultivated on nutrient meat extract agar instead of meat infusion agar. The pullorum antigen strains selected by the Antigen
RMRN9EPJ–. Analysis of development. Embryology; Embryology. Fig. 56. Macroscopic appearance of agglutination reaction in the keyhole limpet Megathura crenulata. Photographed in Syracuse dishes, X Vi- a. Untreated sperm suspension (ca. 2 per cent); b, c, and d, 15 sec- onds, 30 seconds and 10 minutes, respectively, after addition of solution of fertilizin. (After Tyler, '40a.) f^y li v^. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work
RMRE99GE–. Control series bulletin. Feeds -- Analysis Periodicals; Fertilizers -- Analysis Periodicals. 20 (Field Sheet) Owner No. Farm Name P. O. Address Telephone Report to be telephoned Number in flock Breed Total collected Hens Pullets Males Date collected Shipped By Percent pullet production Total new leg bands Season of last test Pail numbers Cartridge numbers Received at laboratory Test fluid Testing Plan Class, of Test Agglutination No. Signed.. Blood Collector. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloratio
RMRHP717–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHANGES IN AGGLUTINATION OF AGEING GERM CELLS. 379. <?))( JTI nouri. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods H
RMRHP73N–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. •>• Lffif io 3 <• 1 a n is ti z? e7 30 • of eati 177 hours FIG. ia. The agglutination cycle when eggs alone were aged, and when such eggs were in good physiologic condition at the time of shedding. Each graph is the average for all females of a series. These graphs show the marked and progressive increase and subsequent decline in values. FIG. ib. The agglutination cycle for ageing eggs when the eggs were overripe at the time of shedding. There is a small or no initial increase, and a slow decrease in values thereaft
RMRHP6XH–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AGGLUTINABILITY OF AGEING SPERM. 397 for female 3, and 93 per cent, for females 4, 5, and 6. The average increase was 71 per cent. In every instance the maxi- mal values did not take place at the initial test but 75 to 90 minutes later. When the experiment was terminated, after 165 minutes, the values were greater than the initial test in 2 batches,. FIG. i. Shows the slow progressive decrease in agglutination values when a suspension of freshly shed sperm is tested at successive intervals with samples of the same egg water.
RMRHP4JH–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AGGLUTINATION PHENOMENON IN CHITON 165 CROZIER, W. J., 1922. An observation on the "cluster-formation" of the sperms of Chiton. Am. Nat., 56: 478-480. FIESER, L. F., 1937. The Chemistry of Natural Products Related to Phenanthrene. Reinhold Publishing Corporation, New York. JUST, E. E., 1915. An experimental analysis of fertilization in Platynereis megalops. Biol. Bull., 28:93-114. JUST, E. E., 1919. The fertilization reaction in Echinarachnius parma. II. The role of fertilizin in straight and cross fertilization. B
RMRN9EPX–. Analysis of development. Embryology; Embryology. 186 Embryogenesis: Preparatory Phases. Fig. 56. Macroscopic appearance of agglutination reaction in the keyhole limpet Megathura crenulata. Photographed in Syracuse dishes, X Vi- a. Untreated sperm suspension (ca. 2 per cent); b, c, and d, 15 sec- onds, 30 seconds and 10 minutes, respectively, after addition of solution of fertilizin. (After Tyler, '40a.) f^y li v^. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may n
RMRHP8YY–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SPERM AGGLUTINATION 167. >/•» t p 'I V ^ v- v.- A b .<--, - ^ **H1'- *^ / V v 'V !. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence,
RMRGBRTG–. Bulletin of the U.S. Department of Agriculture. Agriculture; Agriculture. SJS'''^>mfU Washington, D. C. PROFESSIONAL PAPER February 16, 1917 AN INTRADERMAL TEST FOR BACTERIUM PULLORUM INFECTION IN FOWLS. By Aechibald R. Ward and Bernard A. Gallagher, Pathological Division. CONTENTS. rage. Dissemination of infection in white diarrhea 1 The agglutination test 2 Experimental work 2 Tests of artificially infected birds 2 Field trials of the intradermal tests 10 Page. Comparison of results of agglutina- tion and intradermal tests 12 Significance of swelling as an indi- cation of reaction 13 V
RMRHP8X6–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SPERM AGGLUTINATION 169 proportionality. Starting with a very strong egg water there is, on dilution, at first very little change in rate then a decrease roughly pro- portional to the dilution. With constant egg water concentrations, de- crease in sperm concentration generally causes the rate to pass through a maximum. In addition, concentrated sperm suspensions may fail to give a visible reaction with certain egg water dilutions that do act on more dilute suspensions. TABLE I Time in seconds for agglutination to become macr
RMRHRKF2–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 86 A. J. GOLDFARB. Fig. 31 an agglutination of two eggs. Three agglutinated eggs are shown in Fig. 32 and two and a half eggs in Fig. 33. Clusters. of seven and nine eggs and blastomeres are shown in Figs. 34 and 35.. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original work.. Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole
RMRD6T4R–. Cultural studies of species of Penicillium. Penicillium -- Cultures and culture media; Fungi -- Cultures and culture media. ..;V.;S?f?..fi'.-.';j.i.'. Fig. 15.—PenkUUum roaeum Link (?): o, 6, c, branching of conldlal fructlflcation, showing few cells In ch verticil I < 900); d, e, conidiiferous cell and conidia ( x 900); g, h, j,k, sketches of ripe fructifications showing agglutination of conidia Into slin ,'. slightly apiculate, Bmooth, appearing delicately granular within. Colonies liquefy gelatin cultures rapidlj and give an alkaline reaction to litmus media. Brought from Krai, in Pra
RMRHM77J–. The Biological bulletin. Biology; Zoology; Marine biology. 72 JAMES H. GREGG cells were diluted with about one volume of 0.5% NaCl (approximately 2.5 X 10s cells/ml.) for use in agglutination tests. The cells were placed in agglutination chambers in 10.0-/d. volumes (Gregg and Trygstad, 1958). The antiserum or normal (pre-injection) serum was mixed with the cells in 10.0-/xl. volumes with. Figure la. D. discoideum amoebae + spore antiserum (magnification X36). lb. D. dis- coideum amoebae + spore normal serum (magnification X36). lc. D. discoideum spores + spore antiserum (magnification XlOO)
RMRR24RD–. Advanced biology. Biology; Physiology; Reproduction. 466 TYPHOID FEVER determined by adding blood of a patient who has typhoid, or has recently had it, to some typhoid bacilli on a glass slide. P?^ cover- ^lip. ^,.>i..M::!;'/i^i;i:/, Tignging llrop. The diagram on the left, shows free swimming typhoid bacilli. The one on the right, shows typhoid bacilli clumped in masses by the presence of agglutinins from the blood of a typhoid pa- tient. The agglutination test is usually made in a hanging drop on a glass slide as shown above. When this drop is viewed under the microscope, the germs are
RMRHP6WC–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. .(,(,i ( 11Aisii.i i oi AGEING SPERM. 399 agglutination with ageing sperm occurred not at the initial test but 50 to 125 minutes later. The increase was 35 to 328 per cent. Ageing sperm suspensions gave a similar, progressive and marked increase in agglutination values as did ageing of dry sperm (Goldforb, '29^). Ageing sperm suspensions gave, how- ever, a much quicker increase.. FIG. 2. Shows the slowly increasing agglutination values, when partially over- ripe (6 hours old) dry sperm is used. In Experiment i^A, the age
RMRGMMAX–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. Figure 16. Macroscopic Tube Agglutination Test. Ttie tube on the left shows a negative reaction, and the tube on the right a positive reaction. Refer to question 38. i ! r ⢠I â â .*>w wKtKr ^1 1^ â ^ â Figure 17. The Rapid Serum Agglutination Test. The mixtures'in the squares on the left show a negative reaction, and the mixtures in the squares on the right a pibsitive reaction. Refer to question 39. (18). Please note that these images are extracted from scanned page images that may have been digi
RMRHYNHP–. The beginnings of embryonic development : A symposium organized by the Section on Zoological Sciences of the American Association for the Advancement of Science, cosponsored by the American Society of Zoologists and the Association of Southeastern Biologists, and presented at the Atlanta meeting, December 27, 1955. Embryology. Plate I. Agglutination of Luidia clathrata sperm by versene-egg water. 1, Sperm in sea water. 2, Sperm in 0.0025M versene, pH 8.1. 3, Sperm in egg water. 4, Sperm in versene (0.0025M, pH 8.1)-egg water. 5, Spei-m in versene-egg water. Agglutination appears to be strict
RMRHP74D–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHANGES IN AGGLUTINATION OF AGEING GERM CELLS. 3/7 The evidence demonstrates the cyclical change in agglutinin production by ageing eggs, (2) the cyclical change in agglutina- bility of ageing sperm. (3) When both eggs and sperm aged, r«j. la.. •>• Lffif io 3 <• 1 a n is ti z? e7 30 • of eati 177 hours FIG. ia. The agglutination cycle when eggs alone were aged, and when such eggs were in good physiologic condition at the time of shedding. Each graph is the average for all females of a series. These graphs show the ma
RMRHRKGM–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 84 A. J. GOLDFARB. or extra-ovate as seen in Figs. 15, 16 and 17. In Figs. 17, 18 and 19, the two parts of the egg are equal or almost equal. , It is sometimes difficult to distinguish between an egg with its equal- sized extra-ovate, from one whose two blastomeres have been. 35 34 FIGS. 33-35- partially separated. In either case the volume of the whole egg appears to be increased, sometimes suggesting an agglutination of two whole eggs, as in Figs. 18 and 19. In Fig. 20 two eggs have unmistakably agglutinated. Sometimes thr
RMRJNFDM–. Bacteria : especially as they are related to the economy of nature, to industrial processes and to the public health. Bacteriology; Bacteriology. I ⢠BACILLUS TYPHOSUS (Showing Flagella). BACILLUS TYPHOSUS (Widal Reaction). X iooo. {Agglutination by Serum from Typhoid patient.) X 400.. >' X i / I ⢠% */â¢*"⢠/ BACILLUS COLI COMMUNIS. {From agar culture, 48 hours gro7uth.) X 1000. â ft . /LI % ⢠BACILLUS MYCOIDES. {Spore formation. From aga? culture.) X IOOO. t g* By permission of the Scientific Press, Limited. To face page 64.. Please note that these images are extracted from
RMRGJR8R–. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. W/-£CT£iO OilCKS J iA/fi/C^ MAY SPREAD i/vrecTioH //V MCUBATO/? Cycle, or iNrLcrioN â $s. CHICKS W g/fOO0£/f flOUZEi Figure 1.âPullorum Disease Infection in a Flock May Follow This Cycle. The disease can be eradicated either by breaking the cycle of infection through eliminating the infected birds by means of the macroscopic agglutination test, or by disposing of the entire infected flock and replacing it with pulloruni disease-free stock. '.ssisted by K. I-. Bullis, O. S. Flint, and .Miriam K. Clarke
RMRE99GM–. Control series bulletin. Feeds -- Analysis Periodicals; Fertilizers -- Analysis Periodicals. 17 (Application Card) MASSACHUSETTS AGRICUI/rURAL EXPERIMENT STATION DKPARTMKNT OF VKTKRINARY SCIENCE APPLICATION FOR AGGLUTINATION TEST ON DOMESTIC FOWL Name Tel. No. Date P.O. Address Express Address County Month Test is desired Total birds in flock l)reed and nuiiiher of ) Hens Pullets Males each to l^e tested: ) Do you desire a retest? Siiall your report l)e aixen to tiie County Extension Serice? The Treasurer of the College will not permit additional testing until your account is paid. I promis
RMRHP2XA–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIG. 1. Gross appearance of the egg-agglutination reaction. All photographs natural size. (A) Five drops Arbacla eggs in 3 cc. of 5 per cent sperm extract. Note the drops of agglutinated eggs suspended from the surface of the solution. (B) Ten drops of Arbacia eggs in 3 cc. of 5 per cent sperm extract. The tube was shaken once. The eggs have formed a large dense mass which floats at the surface. (C) Ten drops of Arbacla eggs in 3 cc. heated sea water. The tube was shaken once after adding the eggs. Observe that the ova are h
RMRR303R–. [Publications] - Bureau of Government Laboratories. Biology; Medicine; Natural history. Control Animals IVilhoul Serums. control animals all neg* all dead in 24 hours. control ctftirruzLs all neg; all dead ins 24 hours. Fit frrei/ent Hied afi&r fioe dau/S. Agar* jsl<ate cultuf*es,;fr0pi, all organs were sterile. control animals alt neg; all dead in, 24 hours. conO*ol animals alt neg; all deod in- 24 hours. control aniriLals alt neg; all dead ins 24 hours. Complete agglutination. Ooerlging liquid clear. iprj Aficroscopiealtgf positive 6aciericidal reactions. WJLl Animal alive after 24
RMRHMP39–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 154 IGOR TAMM Lee virus • Virus aErythrocytes MB virus •Virus °Irythrocytes. Win. 20 40 60 80 20 40 60 80 100 120 140 Adsorption I Elution FIGURE 1. Relation of adsorption and elution of virus to agglutination and disagglutination of erythrocytes. With both Lee and MB viruses, adsorption was carried out at 4° C. Elution was permitted to take place at 14° C. with Lee virus, and at 37° C. with MB virus. In both experi- ments the control virus titer was 1024. Chicken erythrocytes, final concentration 5 per cent, were used. e.g.
RMRHP90E–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. m , a b. •v > ir " d FIG. 3. Photomicrographs of sperm of Megathura; all X 300. a and b, Ag- glutinates formed in moderately strong egg water; taken before formation of cen- tral mass; shows tails agglutinated at center and heads clumped in groups (ag- gregated) at periphery, c, Agglutination in weak egg water; from each clump (aggregate) of sperm heads, bundles of tails (not distinctly visible) extend out in several directions and agglutinate with tails from other clumps, d, Aggregation reaction after dilution of sp
RMRHP8Y5–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. >/•» t p 'I V ^ v- v.- A b .<--, - ^ **H1'- *^ / V v 'V !. d FIG. 4. Photomicrographs of sperm of Megathura; X 300; shows types of tail agglutination formed in : a, strong; b, moderately strong; c, weak egg water; from: d and c, " original" head clumps present in sperm suspension. is obtained when strong egg water is added. The free tails come to- gether on one side of the original clump of heads. With moderately strong egg water a great many of the agglutinates, that form from the original sperm clumps, have
RMRGB7H4–. Bulletin of the U.S. Department of Agriculture. Agriculture; Agriculture -- United States. BULLETIN No. 517 Jlr f^^ntrShiitinn frnm e- Riiro»ii nf Animal Induatrv * Contribution from the Bureau of Animal Industry A. D. MELVIN, Chief. s^Ji^'^^^u Washington, D. C. PROFESSIONAL PAPER February 18, 1917 AN INTRADERMAL TEST FOR BACTERIUM PULLORUM INFECTION IN FOWLS. un By Archibald R. Ward and Bek>ard A. Gaxlagheb, Pathological Division. CONTENTS. Dissemination of infection in white diarrhea 1 The agglutination test 2 Experimental work 2 Tests of artificially infected birds 2 Field trials o
RMRHP30B–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 192 JOHN A. FRANK. t PLATE I Microscopic appearance of the egg-agglutination reaction. All eggs photo- graphed in Chinese ink. FIG. 2. Normal .-irbacia egg in heated sea water surrounded by a wide zone of clear, colorless jelly. X 430. FIG. 3. A similar egg after five minutes exposure to a 5 per cent sperm extract. Note that a distinct agglutination membrane has appeared at the periphery of the jelly. X 430. FIG. 4. A similar egg after thirty minutes exposure to a 5 per cent sperm extract. The agglutination membrane has beco
RMRHMR30–. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 5.. r 2 7 FIGURE 6. /5k FIGURE 7. FIGURE 5. Effect of salts on agglutination of washed Chelydra erythrocytes in dextrose solution. Isosmotic salt solutions were added to unbuffered dextrose (0.23 molal) in the pro- portion 1:14. D—dextrose; Na—NaCl + dextrose ; K—KC1 + dextrose; Ca—CaCL + dex- trose; Mg—MgCL + dextrose; R—Ringer (unbuffered). After initial mixing tubes were left undisturbed. 26.5° C. FIGURE 6. Chelydra erythrocytes in 0.23 molal unbuffered dextrose. Washed cells were mixed with dextrose solution and p
RMRKFY9J–. The art of scientific investigation. Research. INDEX. Accidental discoveries, 33 Acid-fast staining, 166 Age, creative, 156 Agglutination, 29 Air sterilisation, 164 Analogy, 94 Anaphylaxis, 28 Aniline dye, 161 Anthrax, 96 Applied research, 126 Attributes for research, 139 Bacon, Francis, 3, 6, 57, 82, 118, Baker, J. R. 74 Balano-posthitis, 165 Bancroft, W. D., 25, 148 Barcroft, Sir Joseph, 11, 153 Bartlett, Sir Frederic, 60 B.C.G. Vaccination, 17 Bennets, H. W., 46, 157 Bernard, Claude, x, 2, 42, 49, 76, 96, 144, 161 Bessemer, 2 Biographies of scientists, 7 Biometrics, 6, 19 Blowfly attack,
RMRGDB1P–. Bulletin of the Southern California Academy of Sciences. Science; Natural history; Natural history. 0.002 0.006 0.010 M cysfeine 20 40 Time in min. 0.014 80- 8 /"v^ % / ^^^^. TR. / ^^X 50' / ^'V 20- / ^ - ^"---—^ 60 90 % TR. 50 20. 20 40 60 time in min. Figure 7. The inhibitory effect of increasing concentrations of cysteine on ag- glutination. Figure 8. The effect of 0.02 M sodium citrate ( ), and phosphate buffer (—) on agglutination. Figure 9. The effect of pH 2.0 ( ), pH 5.0 ( ), and pH 8.3 (—) on agglutination.. Please note that these images are extracted from
RMRGDB35–. Bulletin of the Southern California Academy of Sciences. Science; Natural history; Natural history. 20 time in min 40 60 90 % TR. 50- 20. 20 time in min. 40 60 -). 0.002 M cysteine plus 0.002 Figure 1. The effect of 0.002 M cysteine (— M iodoacetic acid ( ), and phosphate buffer (—) on agglutination. Figure 2. The effect of 0.004 M cysteine (- -), 0.004 M cysteine plus 0.004 M iodoacetic acid ( ), and phosphate buffer (—) on agglutination. Figure 3. The effect of 0.006 M cysteine (- -), 0.006 M cysteine plus 0.006 M iodoacetic acid ( ), and phosphate buffer (—) on agglutination.. Please note
RMRGDB2R–. Bulletin of the Southern California Academy of Sciences. Science; Natural history; Natural history. 92 Bulletin So. Calif. Academy Sciences I Vol. 6j, No. 2, ig68 901 % TR 50- 20-. 20 flme in min. 40 60 90 % TR. 50 20' /' /'' /I 20 time in min. 40 60 90- 6 % TR. 50- r / // / / " — . 20 20 time in min. 40 60 Figure 4. The effect of 0.008 M cysteine ( iodoacetic acid ( ), and phosphate buffer (—) on agglutination. 0.008 M cysteine plus 0.008 M Figure 5. The effect of 0.010 M cysteine (- -), 0.010 M cysteine plus 0.010 M iodoacetic acid ( ), and phosphate buffer (—) on agglutination. Fi
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